depletion  (Sino Biological)


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    Name:
    SARS CoV 2 2019 nCoV Spike RBD His Recombinant Protein COVID 19 Spike RBD Research
    Description:
    A DNA sequence encoding the SARS CoV 2 2019 nCoV Spike Protein RBD YP 009724390 1 Arg319 Phe541 was expressed with a polyhistidine tag at the C terminus
    Catalog Number:
    40592-V08B
    Price:
    None
    Category:
    recombinant protein
    Product Aliases:
    coronavirus spike Protein 2019-nCoV, cov spike Protein 2019-nCoV, ncov RBD Protein 2019-nCoV, ncov s1 Protein 2019-nCoV, ncov s2 Protein 2019-nCoV, ncov spike Protein 2019-nCoV, NCP-CoV RBD Protein 2019-nCoV, NCP-CoV s1 Protein 2019-nCoV, NCP-CoV s2 Protein 2019-nCoV, NCP-CoV Spike Protein 2019-nCoV, novel coronavirus RBD Protein 2019-nCoV, novel coronavirus s1 Protein 2019-nCoV, novel coronavirus s2 Protein 2019-nCoV, novel coronavirus spike Protein 2019-nCoV, RBD Protein 2019-nCoV, S1 Protein 2019-nCoV, S2 Protein 2019-nCoV, Spike RBD Protein 2019-nCoV
    Host:
    Baculovirus-Insect Cells
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    Structured Review

    Sino Biological depletion
    A DNA sequence encoding the SARS CoV 2 2019 nCoV Spike Protein RBD YP 009724390 1 Arg319 Phe541 was expressed with a polyhistidine tag at the C terminus
    https://www.bioz.com/result/depletion/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    depletion - by Bioz Stars, 2021-04
    94/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Disease severity dictates SARS-CoV-2-specific neutralizing antibody responses in COVID-19
    Article Snippet: .. Enzyme-linked immunosorbent assay As previously described, 50 ng of SARS-CoV-2 S1 protein (Sino Biological, 40591-V08H) or SARS-CoV-2 RBD protein (Sino Biological, 40592-V08B) or SARS-CoV-2 S2 protein (Sino Biological, 40590-V08B) in 100 μl phosphate-buffered saline (PBS) per well was coated on ELISA plates (Costar, 42592) overnight at 4 °C. .. The ELISA plates were blocked for 1 h with 100 μl blocking buffer (5% fetal bovine serum (FBS) and 0.1% Tween 20 in PBS) and then incubated with diluted patient or healthy control sera in 100 μl blocking buffer for 1 h. After washing with PBST buffer (0.1% Tween 20 in PBS), the ELISA plates were incubated with anti-human IgG horseradish peroxidase (HRP) antibody (Bioss Biotech, 0297D) for 45 min, followed by PBST washing and addition of 3,3′,5,5′-tetramethylbenzidine (TMB) buffer (Beyotime, P0209).

    Article Title: Disease severity dictates SARS-CoV-2-specific neutralizing antibody responses in COVID-19
    Article Snippet: No reuse allowed without permission. (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. strictly(RBD(dependent(and(deletion(of(RBD_specific(antibodies( led( to( the( failure( in( neutralization(in(S1_specific(sera. (These(results(highlighted(the(importance(of(S1_RBD( itself,(but(not(other(parts(of(S1(protein,(in(inducing(competent(NAbs. ( ( ( In(conclusion,(we(have(demonstrated(the(positive(correlation(between(the(magnitude( of(NAb(responses(and(disease(severity(in(patients(recovered(from(COVID_19. (We(have( also( found( that( disease( severity( also( influences( the( neutralization( heterogeneity( of( SARS_CoV2_specific(antibodies. (Our(results(highlight(the(needs(to(include(mild_illness( and(asymptomatic(patients(for( future(vaccination,(and(also(suggest( the(collection(of( plasma(from(COVID_19(recovered(patients(should(be(restricted(to(those(with(moderate( to( severe( symptoms( for( passive( antibody( therapy. (Our( data( also( provide( important( rationale( for( exclusively( using( SARS_CoV_2( RBD( as( S1_immunogen( in( COVID_19( vaccine(regime. ( . .. MaterialsHuman(The(59(COVID_19( recovered(patients(enrolled( in( the(study(were( provided(written(informed(consent(and(from(different(sources. (The(sera(of(the(severe,( moderate(and(mild(patients(were(obtained(from(Guangzhou(Eighth(People’s(Hospital. ( The(sera(of(the(asymptomatic(patients(were(obtained(from(Chongqing(Public(Health( Medical(Center. (Healthy(control(subjects(were(4(adult(participants( in( the(study. (The( study(received(IRB(approvals(at(Guangzhou(Eighth(People’s(Hospital((KE202001134)( and(Chongqing(Public(Health(Medical(Center((2020_023_01_KY). ( ELISA. (As(previously(described15,(50(ng(of(SARS_CoV_2(S1(protein((Sino(Biological,( 40591_V08H)(or(SARS_CoV_2(RBD(protein((Sino(Biological,(40592_V08B)(or(SARS_ CoV_2(S2(protein((Sino(Biological,(40590_V08B)(in(100(μl(PBS(per(well(was(coated(on( ELISA(plates((Costar,(42592)(overnight(at(4oC. (The(ELISA(plates(were(blocked(for(1( hour( with( 100( μl( blocking( buffer( (5%( FBS( and( 0.1%( Tween( 20( in( PBS)( and( then( incubated(with(diluted(patient(or(healthy(control(sera(in(100(μl(blocking(buffer(for(1(hour. ( All rights reserved. ..

    Multiplex Assay:

    Article Title: SARS-CoV-2-specific antibody detection for sero-epidemiology: a multiplex analysis approach accounting for accurate seroprevalence.
    Article Snippet: Assay ProcedureThe steps in assay validation were similar to recently developed bead-based multiplex immunoassays for CMV, EBV, and RSV, with minor modifications as described below [16, 17]. .. For the multiplex bead-based immune assay the following antigens obtained from Sino Biological were used: SARS-CoV-2 monomeric spike S1 (40591-V08H), RBD (40592-V08B), and nucleoprotein (N) (40588-V08B). ..

    Article Title: SARS-CoV-2–Specific Antibody Detection for Seroepidemiology: A Multiplex Analysis Approach Accounting for Accurate Seroprevalence
    Article Snippet: Assay Procedure The steps in assay validation were similar to recently developed bead-based multiplex immunoassays for CMV, EBV, and RSV, with minor modifications as described below [ , ]. .. For the multiplex bead-based immune assay the following antigens obtained from Sino Biological were used: SARS-CoV-2 monomeric spike S1 (40591-V08H), RBD (40592-V08B), and nucleoprotein (N) (40588-V08B). ..

    Recombinant:

    Article Title: Synthesis of polystyrene-based fluorescent quantum dots nanolabel and its performance in H5N1 virus and SARS-CoV-2 antibody sensing
    Article Snippet: Fluoro-max fluorescent beads with europium chelate was provided from Thermo Fisher Scientific Inc. COOH-coated polystyrene (PS) microbeads (0.2 and 4 μm) were obtained from Huge Biotechnology Co., Ltd (China). .. SARS-CoV-2 (2019-nCoV) Spike RBD-His Recombinant Protein were obtained from Sino Biological (Beijing). .. Influenza A virus H5N1 HA (Hemagglutinin) antibody were purchased from Gene Tex.

    Incubation:

    Article Title: Disease severity dictates SARS-CoV-2-specific neutralizing antibody responses in COVID-19
    Article Snippet: No reuse allowed without permission. (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. after(the(mixture(incubation,(the(luciferase(activity(of(SARS_CoV_2(typed(pseudovirus_ infected(ACE2/293T(cells(were(measured(by(a(luciferase(reporter(assay(kit((Promega,( E1910). ( SARS0CoV02(sera(were(diluted( in(DMEM((40( fold_dilution)( and( mixed( with( an( equal( volume( of( 80_100( PFU( SARS_CoV_2( (EPI_ISL_444969)(for(1(h(at(37°C. (Serum_virus(mixture(were(then(added(to(the(Vero( E6(cell(monolayers(in(48_well(plates(and(incubated(at(37°C(in(5%(CO2(for(1(h.(After( removing(the(inocula,(plates(were(overlaid(with(culture(medium(and(cultured(at(37°C( for(48(h.(Subsequently,(viral(RNA(from(the(cultural(supernatants(was(extracted(and(the( viral(RNA(copies(were(determined(by(quantitative(PCR(according(to(the(viral(detection( kit’s(protocol((DAAN(Gene(Co.,(Ltd.(of(Sun(Yat_Sen(University). (All(experiments(related( to( authentic( viruses( were( performed( in( the( certified( BSL_3( facility( of( Sun( Yat_sen( University.(The(SARS_CoV_2(viral(RNA(fold(reduction(=(2(CT(value(of(sample(_(CT(value(of(mock). .. Depletion(Firstly,(SARS_CoV_2(S1( protein((Sino(Biological,(40591_V08H)(or(SARS_CoV_2(RBD(protein((Sino(Biological,( 40592_V08B)(or(SARS_CoV_2(S2(protein(was(conjugated(with(biotin(by(following(the( manufacture’s( protocol( (Thermo(Fisher(Scientific,(A39257). (Then,( biotin_conjugated( proteins(were(incubated(with(BeaverBeads(Mag(Streptavidin(Matrix((Beaver,(22305)( at(4oC(for(1.5(hours. (After(washing(with(PBS,(the(SARS_CoV_2(S(protein(coupled(beads( were( next( incubated( with( diluted( patient( sera( at( 4oC( for( 1.5( hours. ( Then,( the( supernatants(were(harvested(and(quality(controlled(by(ELISA(assays(for(SARS_CoV_ 2(S(proteins. ( Statistics. (The(SARS_CoV_2(antibody(titers(or(virus(neutralizing(function(of(the(sera( belonging(to(patients(with(different(severity(were(compared(with(the(one_way(ANOVA( test( (Tukey’s( multiple( comparisons( test). ( The( cutoff( value( in( each( pseudovirus( neutralizing(function(assay(was(determined(by(the(ROC(curve(analysis(and(was(of(the( All rights reserved. .. No reuse allowed without permission. (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. highest(likelihood(ratio. (Correlations(between(different(SARS_CoV_2(antibody(titers(or( between(SARS_CoV_2(antibody(titers(and(pseudovirus(titers(or(between(SARS_CoV_2( antibody(titers(and(SARS_CoV_2(virus(titers(were(analyzed(using(Pearson’s(correlation( coefficient. (P( values( less( than( 0.05(were( defied( as( statistically( significant. ( Prism( 6( software(was(used(for(statistical(analysis.

    Article Title: Disease severity dictates SARS-CoV-2-specific neutralizing antibody responses in COVID-19
    Article Snippet: No reuse allowed without permission. (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. strictly(RBD(dependent(and(deletion(of(RBD_specific(antibodies( led( to( the( failure( in( neutralization(in(S1_specific(sera. (These(results(highlighted(the(importance(of(S1_RBD( itself,(but(not(other(parts(of(S1(protein,(in(inducing(competent(NAbs. ( ( ( In(conclusion,(we(have(demonstrated(the(positive(correlation(between(the(magnitude( of(NAb(responses(and(disease(severity(in(patients(recovered(from(COVID_19. (We(have( also( found( that( disease( severity( also( influences( the( neutralization( heterogeneity( of( SARS_CoV2_specific(antibodies. (Our(results(highlight(the(needs(to(include(mild_illness( and(asymptomatic(patients(for( future(vaccination,(and(also(suggest( the(collection(of( plasma(from(COVID_19(recovered(patients(should(be(restricted(to(those(with(moderate( to( severe( symptoms( for( passive( antibody( therapy. (Our( data( also( provide( important( rationale( for( exclusively( using( SARS_CoV_2( RBD( as( S1_immunogen( in( COVID_19( vaccine(regime. ( . .. MaterialsHuman(The(59(COVID_19( recovered(patients(enrolled( in( the(study(were( provided(written(informed(consent(and(from(different(sources. (The(sera(of(the(severe,( moderate(and(mild(patients(were(obtained(from(Guangzhou(Eighth(People’s(Hospital. ( The(sera(of(the(asymptomatic(patients(were(obtained(from(Chongqing(Public(Health( Medical(Center. (Healthy(control(subjects(were(4(adult(participants( in( the(study. (The( study(received(IRB(approvals(at(Guangzhou(Eighth(People’s(Hospital((KE202001134)( and(Chongqing(Public(Health(Medical(Center((2020_023_01_KY). ( ELISA. (As(previously(described15,(50(ng(of(SARS_CoV_2(S1(protein((Sino(Biological,( 40591_V08H)(or(SARS_CoV_2(RBD(protein((Sino(Biological,(40592_V08B)(or(SARS_ CoV_2(S2(protein((Sino(Biological,(40590_V08B)(in(100(μl(PBS(per(well(was(coated(on( ELISA(plates((Costar,(42592)(overnight(at(4oC. (The(ELISA(plates(were(blocked(for(1( hour( with( 100( μl( blocking( buffer( (5%( FBS( and( 0.1%( Tween( 20( in( PBS)( and( then( incubated(with(diluted(patient(or(healthy(control(sera(in(100(μl(blocking(buffer(for(1(hour. ( All rights reserved. ..

    Blocking Assay:

    Article Title: Disease severity dictates SARS-CoV-2-specific neutralizing antibody responses in COVID-19
    Article Snippet: No reuse allowed without permission. (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. strictly(RBD(dependent(and(deletion(of(RBD_specific(antibodies( led( to( the( failure( in( neutralization(in(S1_specific(sera. (These(results(highlighted(the(importance(of(S1_RBD( itself,(but(not(other(parts(of(S1(protein,(in(inducing(competent(NAbs. ( ( ( In(conclusion,(we(have(demonstrated(the(positive(correlation(between(the(magnitude( of(NAb(responses(and(disease(severity(in(patients(recovered(from(COVID_19. (We(have( also( found( that( disease( severity( also( influences( the( neutralization( heterogeneity( of( SARS_CoV2_specific(antibodies. (Our(results(highlight(the(needs(to(include(mild_illness( and(asymptomatic(patients(for( future(vaccination,(and(also(suggest( the(collection(of( plasma(from(COVID_19(recovered(patients(should(be(restricted(to(those(with(moderate( to( severe( symptoms( for( passive( antibody( therapy. (Our( data( also( provide( important( rationale( for( exclusively( using( SARS_CoV_2( RBD( as( S1_immunogen( in( COVID_19( vaccine(regime. ( . .. MaterialsHuman(The(59(COVID_19( recovered(patients(enrolled( in( the(study(were( provided(written(informed(consent(and(from(different(sources. (The(sera(of(the(severe,( moderate(and(mild(patients(were(obtained(from(Guangzhou(Eighth(People’s(Hospital. ( The(sera(of(the(asymptomatic(patients(were(obtained(from(Chongqing(Public(Health( Medical(Center. (Healthy(control(subjects(were(4(adult(participants( in( the(study. (The( study(received(IRB(approvals(at(Guangzhou(Eighth(People’s(Hospital((KE202001134)( and(Chongqing(Public(Health(Medical(Center((2020_023_01_KY). ( ELISA. (As(previously(described15,(50(ng(of(SARS_CoV_2(S1(protein((Sino(Biological,( 40591_V08H)(or(SARS_CoV_2(RBD(protein((Sino(Biological,(40592_V08B)(or(SARS_ CoV_2(S2(protein((Sino(Biological,(40590_V08B)(in(100(μl(PBS(per(well(was(coated(on( ELISA(plates((Costar,(42592)(overnight(at(4oC. (The(ELISA(plates(were(blocked(for(1( hour( with( 100( μl( blocking( buffer( (5%( FBS( and( 0.1%( Tween( 20( in( PBS)( and( then( incubated(with(diluted(patient(or(healthy(control(sera(in(100(μl(blocking(buffer(for(1(hour. ( All rights reserved. ..

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  • 94
    Sino Biological depletion
    Depletion, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/depletion/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    depletion - by Bioz Stars, 2021-04
    94/100 stars
      Buy from Supplier

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