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Biogenex deparaffinized tissue sections
Deparaffinized Tissue Sections, supplied by Biogenex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deparaffinized tissue sections/product/Biogenex
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
deparaffinized tissue sections - by Bioz Stars, 2021-03
86/100 stars

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Immunohistochemistry:

Article Title: Tumor TP53 expression status, body mass index, and prognosis in colorectal cancer
Article Snippet: Tissue microarrays were constructed as previously described. .. For TP53 immunohistochemistry, antigen retrieval was performed using deparaffinized tissue sections in citrate buffer (BioGenex, San Ramon, CA) that were treated in a microwave for 15 minutes. .. Tissue sections were incubated for 10 minutes with 3% H2 O2 (to block endogenous peroxidase), with 10% normal goat serum (Vector Laboratories, Burlingame, CA) in phosphate-buffered saline for 10 minutes.

Article Title: Cyclooxygenase-2 Expression is an Independent Predictor of Poor Prognosis in Colon Cancer
Article Snippet: Our data also indicated that MSI-high or CIMP-high was uncommon (6.3-8.1%) in tumors with p53 positivity in ≥50% tumor cells, while CIMP-high or MSI-high was more frequent (22-27%) in tumors with p53 positivity in < 50% tumor cells as well as tumors without p53 staining. .. For COX-2 immunohistochemistry, antigen retrieval was performed by incubating deparaffinized tissue sections in citrate buffer (BioGenex) by a microwave for 15 min, and let the sections cool for at least 40 min. Tissue sections were incubated with 3% H2 O2 (20 min) to block endogenous peroxidase, and then incubated with Avidin Block (Vector Laboratories) (15 min), than with Biotin Block (Vector Laboratories) (15 min). .. Primary anti-COX-2 antibody (Cayman Chemical, Ann Arbor, MI) (dilution 1:300) was applied for overnight at 4°C.

Incubation:

Article Title: Sildenafil Transiently Delays Early Alveolar Healing of Tooth Extraction Sockets
Article Snippet: Tissue sections were stained with hematoxylin-eosin for histological evaluation, or used for immunohistochemistry. .. Antigen retrieval of deparaffinized tissue sections was done by immersing slides in DeCal Retrieval Solution (Biogenex), 20 m, followed by incubation with anti-von Willebrand Factor (1:250), anti-Ki67 (1:100), anti-Runx2 (1:1000), or anti-Osteopontin (1:2500), 16 h at 4°C. .. Tissue sections were washed and incubated in Biogenex HRP-conjugated universal secondary antibody for 30 m before reacting with diaminobenzidine and counterstaining with hematoxylin.

Article Title: Cyclooxygenase-2 Expression is an Independent Predictor of Poor Prognosis in Colon Cancer
Article Snippet: Our data also indicated that MSI-high or CIMP-high was uncommon (6.3-8.1%) in tumors with p53 positivity in ≥50% tumor cells, while CIMP-high or MSI-high was more frequent (22-27%) in tumors with p53 positivity in < 50% tumor cells as well as tumors without p53 staining. .. For COX-2 immunohistochemistry, antigen retrieval was performed by incubating deparaffinized tissue sections in citrate buffer (BioGenex) by a microwave for 15 min, and let the sections cool for at least 40 min. Tissue sections were incubated with 3% H2 O2 (20 min) to block endogenous peroxidase, and then incubated with Avidin Block (Vector Laboratories) (15 min), than with Biotin Block (Vector Laboratories) (15 min). .. Primary anti-COX-2 antibody (Cayman Chemical, Ann Arbor, MI) (dilution 1:300) was applied for overnight at 4°C.

Article Title: Cyclooxygenase-2 overexpression is common in serrated and non-serrated colorectal adenoma, but uncommon in hyperplastic polyp and sessile serrated polyp/adenoma
Article Snippet: Immunohistochemistry for COX-2 Methods for COX-2 immunohistochemistry were previously described [ , ]. .. Briefly, antigen retrieval was performed by incubating deparaffinized tissue sections in citrate buffer (BioGenex, San Ramon, CA, USA) by a microwave for 15 min. Tissue sections were incubated with 3% H2 O2 (20 min), then with Avidin Block (Vector Laboratories, Burlingame, CA, USA) (15 min), and then with Biotin Block (Vector Laboratories) (15 min). .. Primary anti-COX-2 antibody (Cayman Chemical, Ann Arbor, MI, USA) (dilution 1:300) was applied overnight at 4°C.

Blocking Assay:

Article Title: Cyclooxygenase-2 Expression is an Independent Predictor of Poor Prognosis in Colon Cancer
Article Snippet: Our data also indicated that MSI-high or CIMP-high was uncommon (6.3-8.1%) in tumors with p53 positivity in ≥50% tumor cells, while CIMP-high or MSI-high was more frequent (22-27%) in tumors with p53 positivity in < 50% tumor cells as well as tumors without p53 staining. .. For COX-2 immunohistochemistry, antigen retrieval was performed by incubating deparaffinized tissue sections in citrate buffer (BioGenex) by a microwave for 15 min, and let the sections cool for at least 40 min. Tissue sections were incubated with 3% H2 O2 (20 min) to block endogenous peroxidase, and then incubated with Avidin Block (Vector Laboratories) (15 min), than with Biotin Block (Vector Laboratories) (15 min). .. Primary anti-COX-2 antibody (Cayman Chemical, Ann Arbor, MI) (dilution 1:300) was applied for overnight at 4°C.

Article Title: Cyclooxygenase-2 overexpression is common in serrated and non-serrated colorectal adenoma, but uncommon in hyperplastic polyp and sessile serrated polyp/adenoma
Article Snippet: Immunohistochemistry for COX-2 Methods for COX-2 immunohistochemistry were previously described [ , ]. .. Briefly, antigen retrieval was performed by incubating deparaffinized tissue sections in citrate buffer (BioGenex, San Ramon, CA, USA) by a microwave for 15 min. Tissue sections were incubated with 3% H2 O2 (20 min), then with Avidin Block (Vector Laboratories, Burlingame, CA, USA) (15 min), and then with Biotin Block (Vector Laboratories) (15 min). .. Primary anti-COX-2 antibody (Cayman Chemical, Ann Arbor, MI, USA) (dilution 1:300) was applied overnight at 4°C.

Avidin-Biotin Assay:

Article Title: Cyclooxygenase-2 Expression is an Independent Predictor of Poor Prognosis in Colon Cancer
Article Snippet: Our data also indicated that MSI-high or CIMP-high was uncommon (6.3-8.1%) in tumors with p53 positivity in ≥50% tumor cells, while CIMP-high or MSI-high was more frequent (22-27%) in tumors with p53 positivity in < 50% tumor cells as well as tumors without p53 staining. .. For COX-2 immunohistochemistry, antigen retrieval was performed by incubating deparaffinized tissue sections in citrate buffer (BioGenex) by a microwave for 15 min, and let the sections cool for at least 40 min. Tissue sections were incubated with 3% H2 O2 (20 min) to block endogenous peroxidase, and then incubated with Avidin Block (Vector Laboratories) (15 min), than with Biotin Block (Vector Laboratories) (15 min). .. Primary anti-COX-2 antibody (Cayman Chemical, Ann Arbor, MI) (dilution 1:300) was applied for overnight at 4°C.

Article Title: Cyclooxygenase-2 overexpression is common in serrated and non-serrated colorectal adenoma, but uncommon in hyperplastic polyp and sessile serrated polyp/adenoma
Article Snippet: Immunohistochemistry for COX-2 Methods for COX-2 immunohistochemistry were previously described [ , ]. .. Briefly, antigen retrieval was performed by incubating deparaffinized tissue sections in citrate buffer (BioGenex, San Ramon, CA, USA) by a microwave for 15 min. Tissue sections were incubated with 3% H2 O2 (20 min), then with Avidin Block (Vector Laboratories, Burlingame, CA, USA) (15 min), and then with Biotin Block (Vector Laboratories) (15 min). .. Primary anti-COX-2 antibody (Cayman Chemical, Ann Arbor, MI, USA) (dilution 1:300) was applied overnight at 4°C.

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    Biogenex antigen retrieval
    Antigen Retrieval, supplied by Biogenex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antigen retrieval/product/Biogenex
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antigen retrieval - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

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    Biogenex antigen retrieval citra solution
    Antigen Retrieval Citra Solution, supplied by Biogenex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antigen retrieval citra solution/product/Biogenex
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antigen retrieval citra solution - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

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