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Agilent technologies deparaffinization
Deparaffinization, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deparaffinization/product/Agilent technologies
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
deparaffinization - by Bioz Stars, 2021-03
86/100 stars

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Blocking Assay:

Article Title: Analysis of the Potent Prognostic Factors in Luminal-Type Breast Cancer
Article Snippet: IHC and fluorescence in situ hybridization for HER2/neu IHC of HER2/neu protein were performed on 4 µm thick paraffin embedded tissue sections placed on poly-L-Lysine coated slides. .. After deparaffinization and blocking of the endogenous peroxidase, HER2/neu immunostaining was performed using rabbit anti-human c-erbB-2 oncoprotein as the primary antibody (Dako, Copenhagen, Denmark) at 1:100 dilution. .. Binding of the primary antibody was checked by Dako Quick-Staining, Labelled Streptavidin-Biotin System (LSAB; Dako, Carpentaria, USA), followed by the addition of diaminobenzidine (DAB) as a chromogen.

Article Title: Frequency and Pathological Phenotype of Bovine Astrovirus CH13/NeuroS1 Infection in Neurologically-Diseased Cattle: Towards Assessment of Causality
Article Snippet: .. After deparaffinization, dehydration of the FFPE-tissue and peroxidase blocking in 3% H2 O2 we used microwave heating (20 min at 95 °C) in Dako Target Retrieval solution, pH 9 (Agilent Technologies, Santa Clara, California, USA) as antigen retrieval method. .. Blocking was performed with 10% normal goat serum in phosphate-buffered saline (PBS)-Tween solution, after which the ORF2-con immunoserum (1:100) was added overnight at 4 °C.

Immunostaining:

Article Title: Analysis of the Potent Prognostic Factors in Luminal-Type Breast Cancer
Article Snippet: IHC and fluorescence in situ hybridization for HER2/neu IHC of HER2/neu protein were performed on 4 µm thick paraffin embedded tissue sections placed on poly-L-Lysine coated slides. .. After deparaffinization and blocking of the endogenous peroxidase, HER2/neu immunostaining was performed using rabbit anti-human c-erbB-2 oncoprotein as the primary antibody (Dako, Copenhagen, Denmark) at 1:100 dilution. .. Binding of the primary antibody was checked by Dako Quick-Staining, Labelled Streptavidin-Biotin System (LSAB; Dako, Carpentaria, USA), followed by the addition of diaminobenzidine (DAB) as a chromogen.

Immunohistochemistry:

Article Title: Novel circulating tumor cell-based blood test for the assessment of PD-L1 protein expression in treatment-naïve, newly diagnosed patients with non-small cell lung cancer
Article Snippet: Briefly, tissue sections on glass slides (Mutokagaku, Japan) were preheated prior to IHC staining (Fig. ). .. Upon deparaffinization and antigen retrieval, PD-L1 IHC staining was conducted using the 22C3 clone (22C3 PharmDx Kit, DAKO) on a Ventana BenchMark XT instrument, and the tissue sections were counterstained with hematoxylin. ..

Staining:

Article Title: Novel circulating tumor cell-based blood test for the assessment of PD-L1 protein expression in treatment-naïve, newly diagnosed patients with non-small cell lung cancer
Article Snippet: Briefly, tissue sections on glass slides (Mutokagaku, Japan) were preheated prior to IHC staining (Fig. ). .. Upon deparaffinization and antigen retrieval, PD-L1 IHC staining was conducted using the 22C3 clone (22C3 PharmDx Kit, DAKO) on a Ventana BenchMark XT instrument, and the tissue sections were counterstained with hematoxylin. ..

Formalin-fixed Paraffin-Embedded:

Article Title: Frequency and Pathological Phenotype of Bovine Astrovirus CH13/NeuroS1 Infection in Neurologically-Diseased Cattle: Towards Assessment of Causality
Article Snippet: .. After deparaffinization, dehydration of the FFPE-tissue and peroxidase blocking in 3% H2 O2 we used microwave heating (20 min at 95 °C) in Dako Target Retrieval solution, pH 9 (Agilent Technologies, Santa Clara, California, USA) as antigen retrieval method. .. Blocking was performed with 10% normal goat serum in phosphate-buffered saline (PBS)-Tween solution, after which the ORF2-con immunoserum (1:100) was added overnight at 4 °C.

Article Title: SMAD4 Protein Expression Is Downregulated in Ileal Epithelial Cells from Patients with Crohn's Disease with Significant Inverse Correlation to Disease Activity
Article Snippet: .. Sections from the FFPE terminal ileum biopsies were pretreated by deparaffinization, rehydration, and epitope retrieval in one step (high pH) by the use of “Pretreatment Module” (PT-link; Dako, Glostrup, Denmark) according to the manufacturer's instruction. .. IHC staining was performed using the “Dako Autostainer Link” and with the commercially available kit (EnVision Flex; Dako).

Incubation:

Article Title: Combining radiation with autophagy inhibition enhances suppression of tumor growth and angiogenesis in esophageal cancer
Article Snippet: The samples were assayed for DNA fragmentation [terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay] using the in situ Cell Death Detection kit (Roche Molecular Biochemicals, Indianapolis, IN, USA). .. Briefly, following deparaffinization and dehydration, the tissue sections were incubated in proteinase K (DAKO North America, Inc., Carpinteria, CA, USA) for 15 min, washed with PBS, incubated in equilibration buffer and then in terminal deoxynucleotidyl transferase enzyme solution. .. The sections were subsequently rinsed in PBS, incubated with streptavidin-peroxidase conjugate (Sigma-Aldrich) and visualized using diaminobenzidine (Sigma-Aldrich), according to the manufacturer’s instructions.

Article Title: Comparison of protein expression between formalin-fixed core-cut biopsies and surgical excision specimens using a novel multiplex approach
Article Snippet: Commercial protocols from NanoString for hybridization and detection were followed with minor modifications as follows. .. After deparaffinization and rehydration, epitope retrieval was performed with low pH citrate buffer in a Pre-Treatment-link (Agilent Dako, UK) and samples were incubated overnight with the antibody mix at 4 °C. .. To cleave tags from antibodies, slides were exposed to UV for 3 min in UV Stratalinker 1800 (Strategene, USA).The dilution of nCounter® oligonucleotide tags from the bound antibodies was optimized based on the analysis of 12 samples (6 core-cuts; 6 surgical excisions) run with 3 different dilutions.

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    Agilent technologies deparaffinization
    Deparaffinization, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deparaffinization/product/Agilent technologies
    Average 95 stars, based on 1 article reviews
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    Agilent technologies routine deparaffinization
    Routine Deparaffinization, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/routine deparaffinization/product/Agilent technologies
    Average 93 stars, based on 1 article reviews
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    Agilent technologies deparaffinized sections
    Deparaffinized Sections, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deparaffinized sections/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deparaffinized sections - by Bioz Stars, 2021-03
    86/100 stars
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