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PerkinElmer deoxynucleotide triphosphate
Deoxynucleotide Triphosphate, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxynucleotide triphosphate/product/PerkinElmer
Average 92 stars, based on 32 article reviews
Price from $9.99 to $1999.99
deoxynucleotide triphosphate - by Bioz Stars, 2020-09
92/100 stars

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Polymerase Chain Reaction:

Article Title: Captive Rhesus Monkeys (Macaca mulatta) Are Commonly Infected with Helicobacter cinaedi
Article Snippet: .. Five microliters of supernatant was added to 50-μl reaction volumes containing 5 μl of 10× PCR buffer, 200 μM each deoxynucleotide triphosphate, 0.5 μM each oligonucleotide primer, and 2.5 U of Taq DNA polymerase (Perkin-Elmer Cetus, Norwalk, Conn.). .. Universal 16S ribosomal DNA (rDNA) oligonucleotide primers (5′-AGAGTTTGATCCTGGCTCAG-3′ and 3′-GGTTACCTTGTTACGACTT-5′) were used to amplify an approximately 1.5-kb fragment with a Perkin-Elmer 2400 thermocycler.

Article Title: TNF-? and TNF-? Polymorphisms are Associated with Susceptibility to Osteoarthritis in a Korean Population
Article Snippet: .. We performed each PCR procedure under standard conditions with a 10 µL PCR mixture containing 1 µL of template DNA, 0.5 µM of each primer, 0.2 µM of each deoxynucleotide triphosphate, 1.5 mM of MgCl2 , 0.4 U of the AmpliTaq gold polymerase (Perkin-Elmer), 0.5 µCi of [32 P]dCTP (Amersham, Buckinghamshire, UK), and 1 µL of 10× buffer. ..

Article Title: Subset of Hybrid Eukaryotic Proteins Is Exported by the Type I Secretion System of Erwinia chrysanthemi
Article Snippet: .. PCRs were performed in thin-walled Eppendorf tubes, in a final volume of 100 μl containing 10 μl of 10× PCR buffer (200 mM Tris HCl [pH 8.4], 500 mM KCl), 3 μl of 50 mM MgCl2 , 16 μl of 1.25 mM deoxynucleotide triphosphate, 50 to 75 pmol of each primer, and 2.5 U of AmpliTaq DNA polymerase (Perkin-Elmer). .. Standard conditions were as follows: 30 to 35 cycles of 2 min at 94°C, 1 min at 55°C, and 1 min at 72°C, followed by a terminal elongation step at 72°C for 7 min. Primers for amplification of various fragments are listed in Table .

Article Title: Modulation of Endothelial Cell Function by Normal Polyspecific Human Intravenous Immunoglobulins
Article Snippet: .. All PCRs were carried out in 25 μl of a mixture containing 10 mmol/L deoxynucleotide triphosphate, 1× PCR buffer (10 mmol/L Tris-HCl, pH 8.3, 50 mmol/L KCl, 40% dimethyl sulfoxide, 0.001% gelatin, MgCl2 (see concentrations in Table 1 ), and 2.5 U AmpliTaq polymerase (Perkin-Elmer Corp., Norwalk, CT)). .. Each sample was incubated in a DNA thermal cycler (Perkin-Elmer Corp.) at 52°C to 58°C for 30 to 40 cycles, depending on the primer (Table 1) .

Article Title: No Direct Role for Epstein-Barr Virus in American Hepatocellular Carcinoma
Article Snippet: .. The PCR studies were performed with 2 μl of extracted DNA in a 30-μl mixture containing 50 mmol/L KCl, 10 mmol/L Tris buffer, pH 8.3, 50 μm of each deoxynucleotide triphosphate, 2.5 mmol/L MgCl2 , 1 U of Taq polymerase (Perkin Elmer, Foster City, CA), and 20 pmol of each primer. ..

Concentration Assay:

Article Title: Application of the 5? Fluorogenic Exonuclease Assay (TaqMan) for Quantitative Ribosomal DNA and rRNA Analysis in Sediments
Article Snippet: .. The final reaction conditions were 2 μl of cDNA, 10 mM Tris (pH 8.3), 50 mM KCl, 2.5 mM MgCl2 , 200 μM each deoxynucleotide triphosphate, 0.2 μM forward and reverse primers, and 0.625 U of Taq polymerase (Perkin-Elmer, Foster City, Calif.) which had been pretreated with TaqStart antibody at the recommended concentration (Sigma, St. Louis, Mo.). .. Assembled reaction mixtures were heated to 80°C for 5 min (hot start) and amplified with 5 cycles at 94°C for 40s, 60°C for 10 s, and 72°C for 75 s, followed by 40 cycles at 94°C for 12 s, 65o C for 10 s, and 72°C for 80 s with a 2-s extension per cycle.

Amplification:

Article Title: Nitrate/Nitrite Assimilation System of the Marine Picoplanktonic Cyanobacterium Synechococcus sp. Strain WH 8103: Effect of Nitrogen Source and Availability on Gene Expression
Article Snippet: .. The reaction conditions were as follows: 10 ng of template DNA, 12 pmol of each primer, 1 mM concentrations of each deoxynucleotide triphosphate, and 1 U of AmpliTaq DNA polymerase taken up in 50 μl of amplification buffer containing 1.5 mM Mg2+ (Perkin-Elmer). .. After denaturation at 95°C for 2 min, template DNA was amplified for 25 cycles (94°C for 1 min; 53°C for 1 min; 72°C for 1 min) followed by a final extension at 72°C for 10 min. PCR products were resolved on a 1.0% low-melting-point agarose gel, and fragments of the expected size were ligated into the T-tailed vector pCR2.1 and cloned in the Escherichia coli host strain INVα-F′ supplied with the vector (Invitrogen).

Article Title: Viral Pollution in the Environment and in Shellfish: Human Adenovirus Detection by PCR as an Index of Human Viruses
Article Snippet: .. Amplification was carried out in a 50-μl reaction mixture (100 μl of reaction mixture for shellfish) containing 10 mM Tris-HCl (pH 8.3 at 25°C), 50 mM KCl, 1.5 mM MgCl2 , 200 μM each deoxynucleotide triphosphate, 0.08 μM each adenovirus primer or 0.15 μM the enterovirus or HAV primer, and 2 U of Ampli Taq DNA polymerase (Perkin-Elmer Cetus). .. Thermal cycling of the amplification mixture was performed in a programmable heat block (Gene Amp PCR System 2400; Perkin-Elmer).

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    PerkinElmer deoxynucleoside triphosphate mix
    Deoxynucleoside Triphosphate Mix, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 274 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphate mix/product/PerkinElmer
    Average 92 stars, based on 274 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphate mix - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    92
    PerkinElmer deoxynucleotide triphosphate
    Deoxynucleotide Triphosphate, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleotide triphosphate/product/PerkinElmer
    Average 92 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    deoxynucleotide triphosphate - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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