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Meridian Life Science deoxynucleotide triphosphate
Deoxynucleotide Triphosphate, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 92/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxynucleotide triphosphate/product/Meridian Life Science
Average 92 stars, based on 25 article reviews
Price from $9.99 to $1999.99
deoxynucleotide triphosphate - by Bioz Stars, 2020-09
92/100 stars

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Polymerase Chain Reaction:

Article Title: Arabidopsis Annexin1 Mediates the Radical-Activated Plasma Membrane Ca2+- and K+-Permeable Conductance in Root Cells [W]
Article Snippet: .. The PCR reaction consisted of 100 ng cDNA, 250 nM primers, 250 μM deoxynucleotide triphosphate, 2 mM MgCl2 , 0.625 units of Biotaq DNA polymerase (Bioline), and 17 mM NH4 SO4 , pH 8.8. ..

Article Title: Coniosporium epidermidis sp. nov., a new species from human skin
Article Snippet: .. PCR was performed in 50 μL volumes of a reaction mixture containing 10 mM Tris HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 ·6H2 O, 0.01 % gelatin, 200 mM of each deoxynucleotide triphosphate, 25 pmol of each primer, 10–100 ng rDNA, and 0.5 U Taq DNA polymerase (Bioline, GC Biotech, Alphen a/d Rijn, The Netherlands), as follows: 95 °C for 4 min, followed by 35 cycles consisting of 94 °C for 45 s, 52 °C for 30 s, and 72 °C for 2 min. Amplicons were cleaned with GFX columns (GE Healthcare, Sweden). .. Sequence PCR was performed as follows: 95 °C for one min, followed by 30 cycles consisting of 95 °C for 10 s, 50 °C for five s, and 60 °C for two min. DNA was purified with Sephadex G-50 Superfine.

Article Title: Isolates of Cryptococcus neoformans from Infected Animals Reveal Genetic Exchange in Unisexual, ? Mating Type Populations ▿ from Infected Animals Reveal Genetic Exchange in Unisexual, ? Mating Type Populations ▿ †
Article Snippet: .. PCR amplifications were performed with 50-μl reaction mixtures containing 1× PCR buffer (10 mM Tris-HCl, pH 8.3, 50 mM KCl, 1.5 mM MgCl2 , 0.001% gelatin), 5% glycerol, 0.2 mM deoxynucleotide triphosphate (Bioline, United Kingdom), 0.5 μM concentrations of each primer, 1 μl of Taq DNA polymerase, and 1 μl of template DNA. .. Amplification conditions for PCR were 94°C for 5 min followed by 30 cycles of 94°C for 1 min, 55°C for 1 min, and 72°C for 1 min and a final extension step at 72°C for 7 min. All amplifications were carried out in a PerkinElmer 2400 thermal cycler.

Article Title: Rapid Detection and Identification of Candida, Aspergillus, and Fusarium Species in Ocular Samples Using Nested PCR
Article Snippet: .. Panfungal and nested PCRs were performed in 50-μl reaction volumes containing PCR buffer (50 mM KCl, 10 mM Tris HCl [pH 8.3], 60 ng of each primer, 200 μm (each) deoxynucleotide triphosphate (Bioline, London, United Kingdom), 2.5 mM magnesium chloride , and 2 U of Taq polymerase (Perkin-Elmer, Buckinghamshire, United Kingdom). ..

Article Title: Lack of Association between Genetic Polymorphisms in Enzymes Associated with Folate Metabolism and Unexplained Reduced Sperm Counts
Article Snippet: .. PCR was carried out in a volume of 25 µl containing 30 ng genomic DNA, 1.5 mM MgCl2 , 200 mM each deoxynucleotide triphosphate, 2 µM each primer, 0.5 U Taq DNA polymerase (Bioline, London, U.K.) and 10× reaction buffer. ..

Article Title: Association of Glutathione-S-Transferase (GSTM1 and GSTT1) and FTO Gene Polymorphisms with Type 2 Diabetes Mellitus Cases in Northern India
Article Snippet: .. The PCR reaction was carried out in a total volume of 25 µL containing 10 pmol of each primer, 2.5 mmol/L of MgCl2 , 0.2 mmol/L of each deoxynucleotide triphosphate, 1 unit of Taq polymerase (Bioline Ltd., London, UK) and 100 ng of genomic DNA. .. The amplified products were identified by electrophoresis in a 1.5% agarose gel and stained with 0.5 mg/mL (concentration) ethidium bromide.

Article Title: Isolates of Cryptococcus neoformans from Infected Animals Reveal Genetic Exchange in Unisexual, ? Mating Type Populations ▿ from Infected Animals Reveal Genetic Exchange in Unisexual, ? Mating Type Populations ▿ †
Article Snippet: .. Reactions were done in 100-μl volumes containing 1× PCR buffer, 5% glycerol, 0.2 mM deoxynucleotide triphosphate (Bioline), 0.25 μM concentrations of each primer, 1 μl of Taq DNA polymerase (Roche), and 1 μl of template DNA. .. Amplification conditions for PCR were 94°C for 5 min followed by 35 cycles of 94°C for 1 min, 55°C for 1 min, and 72°C for 1 min, with a final extension step at 72°C for 7 min. All amplifications were carried out in a PerkinElmer 2400 thermal cycler.

Article Title: Laboratory Diagnosis of Common Viral Infections of the Central Nervous System by Using a Single Multiplex PCR Screening Assay
Article Snippet: .. Screen 1 and HSV type-specific primary PCR amplifications were performed in a solution with a total volume of 50 μl containing 20 μl of extracted nucleic acid solution, 16 mM (NH4 )2 SO4 , 67 mM Tris-HCl (pH 8.8 at 25°C), 0.01% (wt/vol) Tween 20 (ammonium sulfate buffer; Bioline Ltd., London, United Kingdom), 1.5 mM MgCl2 , each deoxynucleotide triphosphate (Bioline Ltd.) at a concentration of 0.25 mM, 0.1 μM each oligonucleotide primer (PE Applied Biosystems, Warrington, United Kingdom), and 0.625 U of Taq polymerase (manufacturer’s units; Bioline Ltd.). .. In the screen 1 primary reaction, 0.1 U of Moloney murine leukemia virus reverse transcriptase (manufacturer’s units; Advanced Biotechnologies Ltd., Epsom, United Kingdom) was included for the specific antisense oligonucleotide primed reverse transcription of enterovirus RNA.

Concentration Assay:

Article Title: Laboratory Diagnosis of Common Viral Infections of the Central Nervous System by Using a Single Multiplex PCR Screening Assay
Article Snippet: .. Screen 1 and HSV type-specific primary PCR amplifications were performed in a solution with a total volume of 50 μl containing 20 μl of extracted nucleic acid solution, 16 mM (NH4 )2 SO4 , 67 mM Tris-HCl (pH 8.8 at 25°C), 0.01% (wt/vol) Tween 20 (ammonium sulfate buffer; Bioline Ltd., London, United Kingdom), 1.5 mM MgCl2 , each deoxynucleotide triphosphate (Bioline Ltd.) at a concentration of 0.25 mM, 0.1 μM each oligonucleotide primer (PE Applied Biosystems, Warrington, United Kingdom), and 0.625 U of Taq polymerase (manufacturer’s units; Bioline Ltd.). .. In the screen 1 primary reaction, 0.1 U of Moloney murine leukemia virus reverse transcriptase (manufacturer’s units; Advanced Biotechnologies Ltd., Epsom, United Kingdom) was included for the specific antisense oligonucleotide primed reverse transcription of enterovirus RNA.

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    Meridian Life Science monoclonal antibody against dt
    Monoclonal Antibody Against Dt, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal antibody against dt/product/Meridian Life Science
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal antibody against dt - by Bioz Stars, 2020-09
    85/100 stars
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