Structured Review

Kaneka Corp deoxynucleotide triphosphate
Deoxynucleotide Triphosphate, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxynucleotide triphosphate/product/Kaneka Corp
Average 90 stars, based on 7 article reviews
Price from $9.99 to $1999.99
deoxynucleotide triphosphate - by Bioz Stars, 2020-09
90/100 stars

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Polymerase Chain Reaction:

Article Title: Recurrent DNA virus domestication leading to different parasite virulence strategies
Article Snippet: .. A 30-cycle PCR (94°C for 60 s, 58°C for 60 s, and 72°C for 60 s) was performed with 10 pmol of each primer, 0.2 mM deoxynucleotide triphosphate (dNTP; MP Biochemicals), 1.5 mM MgCl2 , 0.5 U of Goldstar (Eurogentec), and 1 μl of total DNA. .. PCR products (8 μl) were run on 2% agarose gels.

Article Title: Discrimination of Psychrotrophic and Mesophilic Strains of the Bacillus cereus Group by PCR Targeting of Major Cold Shock Protein Genes
Article Snippet: .. Reactions were carried out in 50-μl volumes containing 5 μl of 10× PCR buffer (supplied with Taq DNA polymerase; Eurogentec), 2.0 mM MgCl2 , 50 pmol of each oligonucleotide primer (see below), 0.2 mM each deoxynucleotide triphosphate (dATP, dCTP, dGTP, and dTTP; Eurogentec), 1 U of Taq DNA polymerase (Eurogentec), and a pinhead-sized aliquot of bacteria picked from a PEMBA plate. .. Bacterial cells were lysed by heating the mixture at 95°C for 5 min. Amplification of cspA was then attempted, with 30 cycles at 95°C for 15 s, 50°C for 30 s, and 72°C for 30 s, followed by a final extension step at 72°C for 2 min.

Article Title: Pathogenic Yersinia Species Carry a Novel, Cold-Inducible Major Cold Shock Protein Tandem Gene Duplication Producing both Bicistronic and Monocistronic mRNA
Article Snippet: .. Reactions were carried out in 50-μl volumes containing 5 μl of 10× PCR buffer (supplied with Taq DNA polymerase; Eurogentec), 2 mM MgCl2 , 100 pmol of each oligonucleotide primer, 0.2 mM each deoxynucleotide triphosphate (dATP, dCTP, dGTP, dTTP), 1 U of Taq DNA polymerase (Eurogentec), and 1 μl of a ligation mixture or uncut DNA. ..

Article Title: Characterization of a Protocatechuate Catabolic Gene Cluster from Rhodococcus opacus 1CP: Evidence for a Merged Enzyme with 4-Carboxymuconolactone-Decarboxylating and 3-Oxoadipate Enol-Lactone-Hydrolyzing Activity
Article Snippet: .. PCR mixtures (50 μl) for the amplification of genomic DNA contained 50 pmol of each primer, 0.1 to 0.2 μg of genomic template DNA, 0.1 mM each deoxynucleotide triphosphate, Goldstar polymerase reaction buffer [75 mM Tris-HCl (pH 9.0), 20 mM (NH4 )2 SO4 , 0.01% (wt/vol) Tween 20], 1.5 mM MgCl2 , and 0.5 U of Eurogentech Goldstar DNA polymerase. .. The PCR was performed with a touchdown thermocycle program ( ): initial denaturation (94°C; 3 min) before addition of the polymerase; 6 cycles with decreasing annealing temperature (66 to 56°C; 1 min), polymerization (72°C; initially 1 min, plus 1 additional s for each cycle), and denaturation (94°C; 30 s); 28 more cycles with 54°C as the annealing temperature; an additional 14 min of polymerization during the last cycle.

Random Hexamer Labeling:

Article Title: Zoledronic acid up-regulates bone sialoprotein expression in osteoblastic cells through Rho GTPase inhibition
Article Snippet: .. First-strand cDNA was synthesized using 2 μg of total RNA in 20 μl of RT reaction mixture containing 0.2 μg of pd(N)6 random hexamer (Amersham Biosciences, Little Chalfont, Bucks., U.K.), 2 mM of each deoxynucleotide triphosphate (Eurogentec, Seraing, Belgium), 1× first-strand buffer [50 mM Tris/HCl (pH 8.3), 75 mM KCl, 3 mM MgCl2 ] (Invitrogen), 10 mM dithiothreitol (Invitrogen), and 100 units of SuperScript™ II RNase H RT (Invitrogen). ..

Amplification:

Article Title: Insecticide resistance status of the Anopheles funestus population in Central African Republic: a challenge in the war
Article Snippet: .. Briefly, the ITS2 and D3 regions of the rDNA were amplified from 20 ng of template DNA in 50 μl reaction mixture containing 5 μl of 10× reaction buffer (Qiagen), 1.5 mM MgCl2 , 200 μM each deoxynucleotide triphosphate (Eurogentec), 0.5 units of Taq DNA polymerase (Qiagen), and 20 pmol each of forward and reverse primers. ..

Article Title: Characterization of a Protocatechuate Catabolic Gene Cluster from Rhodococcus opacus 1CP: Evidence for a Merged Enzyme with 4-Carboxymuconolactone-Decarboxylating and 3-Oxoadipate Enol-Lactone-Hydrolyzing Activity
Article Snippet: .. PCR mixtures (50 μl) for the amplification of genomic DNA contained 50 pmol of each primer, 0.1 to 0.2 μg of genomic template DNA, 0.1 mM each deoxynucleotide triphosphate, Goldstar polymerase reaction buffer [75 mM Tris-HCl (pH 9.0), 20 mM (NH4 )2 SO4 , 0.01% (wt/vol) Tween 20], 1.5 mM MgCl2 , and 0.5 U of Eurogentech Goldstar DNA polymerase. .. The PCR was performed with a touchdown thermocycle program ( ): initial denaturation (94°C; 3 min) before addition of the polymerase; 6 cycles with decreasing annealing temperature (66 to 56°C; 1 min), polymerization (72°C; initially 1 min, plus 1 additional s for each cycle), and denaturation (94°C; 30 s); 28 more cycles with 54°C as the annealing temperature; an additional 14 min of polymerization during the last cycle.

Synthesized:

Article Title: Zoledronic acid up-regulates bone sialoprotein expression in osteoblastic cells through Rho GTPase inhibition
Article Snippet: .. First-strand cDNA was synthesized using 2 μg of total RNA in 20 μl of RT reaction mixture containing 0.2 μg of pd(N)6 random hexamer (Amersham Biosciences, Little Chalfont, Bucks., U.K.), 2 mM of each deoxynucleotide triphosphate (Eurogentec, Seraing, Belgium), 1× first-strand buffer [50 mM Tris/HCl (pH 8.3), 75 mM KCl, 3 mM MgCl2 ] (Invitrogen), 10 mM dithiothreitol (Invitrogen), and 100 units of SuperScript™ II RNase H RT (Invitrogen). ..

Ligation:

Article Title: Pathogenic Yersinia Species Carry a Novel, Cold-Inducible Major Cold Shock Protein Tandem Gene Duplication Producing both Bicistronic and Monocistronic mRNA
Article Snippet: .. Reactions were carried out in 50-μl volumes containing 5 μl of 10× PCR buffer (supplied with Taq DNA polymerase; Eurogentec), 2 mM MgCl2 , 100 pmol of each oligonucleotide primer, 0.2 mM each deoxynucleotide triphosphate (dATP, dCTP, dGTP, dTTP), 1 U of Taq DNA polymerase (Eurogentec), and 1 μl of a ligation mixture or uncut DNA. ..

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    Kaneka Corp sirna duplex
    Sirna Duplex, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sirna duplex/product/Kaneka Corp
    Average 92 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    sirna duplex - by Bioz Stars, 2020-09
    92/100 stars
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