Structured Review

GE Healthcare deoxynucleotide triphosphate
Deoxynucleotide Triphosphate, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxynucleotide triphosphate/product/GE Healthcare
Average 92 stars, based on 25 article reviews
Price from $9.99 to $1999.99
deoxynucleotide triphosphate - by Bioz Stars, 2020-07
92/100 stars

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Polymerase Chain Reaction:

Article Title: Uncoupling of Myelin Assembly and Schwann Cell Differentiation by Transgenic Overexpression of Peripheral Myelin Protein 22
Article Snippet: .. All PCR amplifications were semiquantitative, performed in 4 × 20 μl reaction buffer, containing 2 ng of cDNA, 400 n m of each primer, 200 μ m of each deoxynucleotide triphosphate, 3 μ m MgCl, and 1 U of Taq polymerase (Amersham Pharmacia Biotech, Uppsala, Sweden) from the same master mix. .. To amplify SCIP cDNA, PCR was performed in the presence of 6 μ m MgCl and 10% DMSO.

Article Title: Mutations in the ST7/RAY1/HELG locus rarely occur in primary colorectal, gastric, and hepatocellular carcinomas
Article Snippet: .. The genomic DNA template (50 ng) was incubated in a total volume of 10 μ l PCR buffer that contained 10 mM Tris-HCl (pH 9.0), 50 mM KCl, 1.5 mM MgCl2 , 100 nM of each primer, 200 μ M of each deoxynucleotide triphosphate, 1.5 Ci of alpha-[32 P]dCTP (Amersham Pharmacia), and 0.5 U of rTaq DNA polymerase (TaKaRa). .. The following PCR conditions were employed: 30 s at 95°C, 30 s at 58°C or 62°C, and 90 s at 72°C for 35 cycles, followed by 10 min at 72°C in a thermal cycler (GeneAmp 9700; Applied Biosystems).

Article Title: Emergence of Reduced Susceptibility and Resistance to Fluoroquinolones in Escherichia coli in Taiwan and Contributions of Distinct Selective Pressures
Article Snippet: .. Amplifications were carried out with a Peltier Thermal Cycler PTC-200 PCR System (MJ Research Inc., Waltham, Mass.) in 50-μl volumes containing 50 pmol of each primer, 200 μM deoxynucleotide triphosphate, 1× reaction buffer (10 mM Tris-HCl [pH 9], 50 mM KCl, 1.5 mM MgCl2 ), 1 U of Taq polymerase (Amersham Pharmacia Biotech Inc., Uppsala, Sweden), and 10 μl of diluted cell lysate. .. An initial 5-min period of denaturation at 95°C was followed by 35 cycles of denaturation (1 min at 94°C), annealing (1 min at 60°C), and extension (1 min at 72°C), followed by a final cycle of 72°C for 15 min. Agarose gel electrophoresis and ethidium bromide staining to confirm the sizes of the gene fragments were used to visualize the amplification products.

Article Title: Loss of CDX2 Expression and Microsatellite Instability Are Prominent Features of Large Cell Minimally Differentiated Carcinomas of the Colon
Article Snippet: .. PCR was performed in 20-μl reaction mixtures containing 20 mmol/L Tris-HCl (pH 8.4), 50 mmol/L KCl, 1.5 mmol/L MgCl2 , 200 nmol/L of each primer, 200 μmol/L of each deoxynucleotide triphosphate, 1.5 μCi of[α-32 P]dCTP (Amersham Pharmacia Biotech), and 0.25 U of Taq DNA polymerase. .. DNA fragments for each microsatellite locus were amplified for 35 cycles of 94°C for 60 seconds, 55°C for 60 seconds, and 72°C for 90 seconds followed by a final extension for 10 minutes at 72°C.

Concentration Assay:

Article Title: Bioluminescence imaging of estrogen receptor activity during breast cancer progression
Article Snippet: .. Deoxynucleotide triphosphate (GE Healthcare) and Moloney murine leukemia virus reverse transcriptase (RT; Promega) were added at 0.5 mM and 8 U/µl final concentration, respectively, in a final volume of 25 µl. ..

Incubation:

Article Title: Mutations in the ST7/RAY1/HELG locus rarely occur in primary colorectal, gastric, and hepatocellular carcinomas
Article Snippet: .. The genomic DNA template (50 ng) was incubated in a total volume of 10 μ l PCR buffer that contained 10 mM Tris-HCl (pH 9.0), 50 mM KCl, 1.5 mM MgCl2 , 100 nM of each primer, 200 μ M of each deoxynucleotide triphosphate, 1.5 Ci of alpha-[32 P]dCTP (Amersham Pharmacia), and 0.5 U of rTaq DNA polymerase (TaKaRa). .. The following PCR conditions were employed: 30 s at 95°C, 30 s at 58°C or 62°C, and 90 s at 72°C for 35 cycles, followed by 10 min at 72°C in a thermal cycler (GeneAmp 9700; Applied Biosystems).

Synthesized:

Article Title: Arabidopsis RTM2 Gene Is Necessary for Specific Restriction of Tobacco Etch Virus and Encodes an Unusual Small Heat Shock-like Protein
Article Snippet: .. First-strand cDNA was synthesized for 1 hr at 42°C in reactions (10 μL) containing ∼200 ng of poly(A)+ RNA, 0.5 μg/mL oligo dT primer, 5 pmol/μL RTM2-RTR primer (5′-CTCCTCTAGCTTCGCCGCCTTCTC-3′), buffer (50 mM Tris-HCl, pH 8.3, 75 mM KCl, and 3 mM MgCl2 ), 20 mM DTT, 1 mM each deoxynucleotide triphosphate, 2 μCi 32 P-dATP (3000 Ci/mM; Amersham), and 200 units of Superscript II reverse transcriptase (Gibco BRL). .. Unincorporated nucleotides were removed by filtration on Sephadex G50 centrifuge columns (Boehringer Mannheim).

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    GE Healthcare deoxynucleoside triphosphate
    Deoxynucleoside Triphosphate, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphate/product/GE Healthcare
    Average 92 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphate - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    GE Healthcare deoxynucleotide triphosphate
    Deoxynucleotide Triphosphate, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleotide triphosphate/product/GE Healthcare
    Average 92 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    deoxynucleotide triphosphate - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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