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GE Healthcare deoxynucleotide triphosphate
Deoxynucleotide Triphosphate, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxynucleotide triphosphate/product/GE Healthcare
Average 92 stars, based on 25 article reviews
Price from $9.99 to $1999.99
deoxynucleotide triphosphate - by Bioz Stars, 2020-10
92/100 stars

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Polymerase Chain Reaction:

Article Title: Uncoupling of Myelin Assembly and Schwann Cell Differentiation by Transgenic Overexpression of Peripheral Myelin Protein 22
Article Snippet: .. All PCR amplifications were semiquantitative, performed in 4 × 20 μl reaction buffer, containing 2 ng of cDNA, 400 n m of each primer, 200 μ m of each deoxynucleotide triphosphate, 3 μ m MgCl, and 1 U of Taq polymerase (Amersham Pharmacia Biotech, Uppsala, Sweden) from the same master mix. .. To amplify SCIP cDNA, PCR was performed in the presence of 6 μ m MgCl and 10% DMSO.

Article Title: Vacuolating encephalitis in mice infected by human coronavirus OC43.
Article Snippet: .. Approximately 5 g of RNA was reverse transcribed with Expand Moloney murine leukemia virus reverse transcriptase (Gibco-BRL) and the cDNA products were incubated in 20 pmol of each sense and antisense primers, 2.5 mM MgCl 2 , 1ϫ PCR buffer (10 mM Tris-HCl, pH 8.3; 50 mM KCl), and 0.4 mM of each deoxynucleotide triphosphate, heated at 94°C for 5 min and 60°C (HCoV-OC43) or 50°C (GAPDH and MHV) for 5 min. After the addition of Expand high-fidelity PCR system DNA polymerase (rTaq, 5000 U/l; Amersham Pharmacia Biotech Inc., Baie d'Urfé, QC), 30 amplification cycles of 2 min at 72°C, 1 min at 95°C, and 2 min at 60°C (HCoV-OC43) or 50°C (GAPDH and MHV) were performed. .. Ten microliters of this reaction mix was loaded onto a 1.2% (w/v) agarose gel containing 5 l ethidium bromide.

Incubation:

Article Title: Vacuolating encephalitis in mice infected by human coronavirus OC43.
Article Snippet: .. Approximately 5 g of RNA was reverse transcribed with Expand Moloney murine leukemia virus reverse transcriptase (Gibco-BRL) and the cDNA products were incubated in 20 pmol of each sense and antisense primers, 2.5 mM MgCl 2 , 1ϫ PCR buffer (10 mM Tris-HCl, pH 8.3; 50 mM KCl), and 0.4 mM of each deoxynucleotide triphosphate, heated at 94°C for 5 min and 60°C (HCoV-OC43) or 50°C (GAPDH and MHV) for 5 min. After the addition of Expand high-fidelity PCR system DNA polymerase (rTaq, 5000 U/l; Amersham Pharmacia Biotech Inc., Baie d'Urfé, QC), 30 amplification cycles of 2 min at 72°C, 1 min at 95°C, and 2 min at 60°C (HCoV-OC43) or 50°C (GAPDH and MHV) were performed. .. Ten microliters of this reaction mix was loaded onto a 1.2% (w/v) agarose gel containing 5 l ethidium bromide.

Amplification:

Article Title: Vacuolating encephalitis in mice infected by human coronavirus OC43.
Article Snippet: .. Approximately 5 g of RNA was reverse transcribed with Expand Moloney murine leukemia virus reverse transcriptase (Gibco-BRL) and the cDNA products were incubated in 20 pmol of each sense and antisense primers, 2.5 mM MgCl 2 , 1ϫ PCR buffer (10 mM Tris-HCl, pH 8.3; 50 mM KCl), and 0.4 mM of each deoxynucleotide triphosphate, heated at 94°C for 5 min and 60°C (HCoV-OC43) or 50°C (GAPDH and MHV) for 5 min. After the addition of Expand high-fidelity PCR system DNA polymerase (rTaq, 5000 U/l; Amersham Pharmacia Biotech Inc., Baie d'Urfé, QC), 30 amplification cycles of 2 min at 72°C, 1 min at 95°C, and 2 min at 60°C (HCoV-OC43) or 50°C (GAPDH and MHV) were performed. .. Ten microliters of this reaction mix was loaded onto a 1.2% (w/v) agarose gel containing 5 l ethidium bromide.

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    GE Healthcare deoxynucleoside triphosphate
    Deoxynucleoside Triphosphate, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphate/product/GE Healthcare
    Average 94 stars, based on 48 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphate - by Bioz Stars, 2020-10
    94/100 stars
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