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Eppendorf AG deoxynucleotide triphosphate
Deoxynucleotide Triphosphate, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxynucleotide triphosphate/product/Eppendorf AG
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
deoxynucleotide triphosphate - by Bioz Stars, 2020-09
92/100 stars

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High Performance Liquid Chromatography:

Article Title: Human Cytomegalovirus: detection of congenital and perinatal infection in Argentina
Article Snippet: .. Reaction mixtures consisted of 5 μl of target DNA, 10 pmol of each oligonucleotide primer, for LA primers 2.5 U/μl of enzyme Taq polymerase (Perkin-Elmer Cetus, Nowalk) and 2.0 U/μl for gB and IE primers, 200 μM (each) of deoxynucleotide triphosphate, 5 μl of 10X reaction buffer (500 mM KCl, 10 mM tris-HCl, pH 8.3, 1.5 mM MgCl2 , 0.01% gelatin), and high-pressure liquid chromatography distilled water to a total volume of 50 μl in an Eppendorf tube. ..

Amplification:

Article Title: Hemoculture and Polymerase Chain Reaction Using Primers TCZ1/TCZ2 for the Diagnosis of Canine and Feline Trypanosomiasis
Article Snippet: .. All reactions were performed in duplicate, containing 2.5 μ L of PCR buffer (50 mmol KCl, 10 mmol of Tris-HCl, and 1.5 mM MgCl2 ), 0.2 mM of each deoxynucleotide triphosphate, 1.0 U of Taq -polymerase, 10 pmol of each primer, 2 μ L of DNA, and 17.8 μ L of ultrapure water for a final volume of 25 μ L. The conditions of amplification in a thermocycler (Mastercycler Personal, Eppendorf) occurred according to Virreira et al. [ ] as follows: one cycle for initial denaturation at 94°C for 5 minutes; 35 amplification cycles (denaturation) at 94°C for 20 seconds, annealing at 57°C for 10 seconds and extension at 72°C for 30 seconds, and one cycle of 72°C for seven minutes for terminal extension, respectively. .. The 10 μ L aliquots of the samples amplified by using primers TCZ1/TCZ2 were homogenized with 2 μ L of bromophenol blue solution, submitted to horizontal agarose gel electrophoresis at 10% in 10x tris-borate-EDTA (TBE) buffer and stained by Gelred (Biotium, Inc.) for identification of the amplified products.

Article Title: Celecoxib promotes c-FLIP degradation through Akt-independent inhibition of GSK3
Article Snippet: .. The 25-µL amplification mixture contained 1 µL of the cDNA, 0.5 µL of deoxynucleotide triphosphate (25 mM each), 1 µL each of the sense and antisense primers (20 µM each), 5 µL of TaqMaster PCR enhancer, 1 µL of Taq DNA polymerase (5 units/µL; Eppendorf), 2.5 µL 10 × reaction buffer, and sterile H2 O. PCR was performed for 35 cycles. ..

Synthesized:

Article Title: NPM1 Silencing Reduces Tumour Growth and MAPK Signalling in Prostate Cancer Cells
Article Snippet: .. Preparation of RNA and real-time quantitative PCR Total cellular RNA was extracted using TRIzol reagent (Life Technologies) and cDNA was synthesized with 200 U of Moloney murine leukemia virus-reverse transcriptase (Promega), 5 pmol of random primers (C1181, Promega), 40 U RNAsin (Promega), and 2.5 mM deoxynucleotide triphosphate. mRNA levels were quantitated on a Mastercycler ep Realplex (MasterCycler2, Eppendorf, Le Pecq, France) with Mesagreen QPCR Mastermix Plus for SYBR (Promega). .. Sequences of the primers are the following: NPM1, 5′-ATGGAAGATTCGATGGACATGG-3′ (Forward) and 5′-CGAGAAGAGACTTCCTCCACTGC -3′ (Reverse); PCNA, 5′-TGCCTTCTGGTGAATTTGCACGT-3′ (Forward) and 5′- ACCGTTGAAGAGAGTGGAGTGGC-3′ (Reverse), Actin, 5′-CGCGAGAAGATGACCCAGATC-3′ (Forward) and 5′TCACCGGAGTCCATCACGA-3′ (Reverse) (Eurogentec, Angers, France).

Real-time Polymerase Chain Reaction:

Article Title: NPM1 Silencing Reduces Tumour Growth and MAPK Signalling in Prostate Cancer Cells
Article Snippet: .. Preparation of RNA and real-time quantitative PCR Total cellular RNA was extracted using TRIzol reagent (Life Technologies) and cDNA was synthesized with 200 U of Moloney murine leukemia virus-reverse transcriptase (Promega), 5 pmol of random primers (C1181, Promega), 40 U RNAsin (Promega), and 2.5 mM deoxynucleotide triphosphate. mRNA levels were quantitated on a Mastercycler ep Realplex (MasterCycler2, Eppendorf, Le Pecq, France) with Mesagreen QPCR Mastermix Plus for SYBR (Promega). .. Sequences of the primers are the following: NPM1, 5′-ATGGAAGATTCGATGGACATGG-3′ (Forward) and 5′-CGAGAAGAGACTTCCTCCACTGC -3′ (Reverse); PCNA, 5′-TGCCTTCTGGTGAATTTGCACGT-3′ (Forward) and 5′- ACCGTTGAAGAGAGTGGAGTGGC-3′ (Reverse), Actin, 5′-CGCGAGAAGATGACCCAGATC-3′ (Forward) and 5′TCACCGGAGTCCATCACGA-3′ (Reverse) (Eurogentec, Angers, France).

Polymerase Chain Reaction:

Article Title: Diversity, Abundance, and Potential Activity of Nitrifying and Nitrate-Reducing Microbial Assemblages in a Subglacial Ecosystem ▿Diversity, Abundance, and Potential Activity of Nitrifying and Nitrate-Reducing Microbial Assemblages in a Subglacial Ecosystem ▿ †
Article Snippet: .. The final reaction mixture (50 μl) contained 2 mM MgCl2 (Invitrogen), 200 μM each deoxynucleotide triphosphate (Eppendorf, Hamburg, Germany), 0.5 μM each forward and reverse primer, 0.4 mg ml−1 molecular-grade bovine serum albumin (Roche, Indianapolis, IN), and 0.25 units of Taq DNA polymerase (Invitrogen) in 1× PCR buffer (Invitrogen). .. An equal volume of each replicate PCR amplification using DNA from RE ( ) sampled in July 2008 was pooled, purified, and subjected to sequence analysis according to previously described protocols ( ).

Article Title: Hemoculture and Polymerase Chain Reaction Using Primers TCZ1/TCZ2 for the Diagnosis of Canine and Feline Trypanosomiasis
Article Snippet: .. All reactions were performed in duplicate, containing 2.5 μ L of PCR buffer (50 mmol KCl, 10 mmol of Tris-HCl, and 1.5 mM MgCl2 ), 0.2 mM of each deoxynucleotide triphosphate, 1.0 U of Taq -polymerase, 10 pmol of each primer, 2 μ L of DNA, and 17.8 μ L of ultrapure water for a final volume of 25 μ L. The conditions of amplification in a thermocycler (Mastercycler Personal, Eppendorf) occurred according to Virreira et al. [ ] as follows: one cycle for initial denaturation at 94°C for 5 minutes; 35 amplification cycles (denaturation) at 94°C for 20 seconds, annealing at 57°C for 10 seconds and extension at 72°C for 30 seconds, and one cycle of 72°C for seven minutes for terminal extension, respectively. .. The 10 μ L aliquots of the samples amplified by using primers TCZ1/TCZ2 were homogenized with 2 μ L of bromophenol blue solution, submitted to horizontal agarose gel electrophoresis at 10% in 10x tris-borate-EDTA (TBE) buffer and stained by Gelred (Biotium, Inc.) for identification of the amplified products.

Article Title: Celecoxib promotes c-FLIP degradation through Akt-independent inhibition of GSK3
Article Snippet: .. The 25-µL amplification mixture contained 1 µL of the cDNA, 0.5 µL of deoxynucleotide triphosphate (25 mM each), 1 µL each of the sense and antisense primers (20 µM each), 5 µL of TaqMaster PCR enhancer, 1 µL of Taq DNA polymerase (5 units/µL; Eppendorf), 2.5 µL 10 × reaction buffer, and sterile H2 O. PCR was performed for 35 cycles. ..

IA:

Article Title: The Role of Tetraether Lipid Composition in the Adaptation of Thermophilic Archaea to Acidity
Article Snippet: .. Reactions contained 2 mM MgCl2 (Invitrogen, Carlsbad, CA, USA), 200 μM each deoxynucleotide triphosphate (Eppendorf, Hamburg, Germany), 0.5 μM forward and reverse primer (Integrated DNA Technologies, Coralville, IA, USA), 0.4 mg mL−1 molecular-grade bovine serum albumin (Roche, Indianapolis, IN, USA) and 0.25 units Taq DNA polymerase (Invitrogen, Carlsbad, CA, USA) in a final reaction volume of 50 μL. .. An equal volume of each triplicate reaction was pooled and purified using a QIA quick PCR Purification Kit (Qiagen, Valencia, CA, USA).

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  • 89
    Eppendorf AG deoxynucleotide triphosphate mixture
    Deoxynucleotide Triphosphate Mixture, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleotide triphosphate mixture/product/Eppendorf AG
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deoxynucleotide triphosphate mixture - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

    92
    Eppendorf AG deoxynucleotide triphosphate
    Deoxynucleotide Triphosphate, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleotide triphosphate/product/Eppendorf AG
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    deoxynucleotide triphosphate - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    92
    Eppendorf AG deoxynucleoside triphosphate mixture
    Deoxynucleoside Triphosphate Mixture, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphate mixture/product/Eppendorf AG
    Average 92 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphate mixture - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    92
    Eppendorf AG deoxynucleoside triphosphate
    Deoxynucleoside Triphosphate, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphate/product/Eppendorf AG
    Average 92 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphate - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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