deoxynucleoside triphosphates  (Thermo Fisher)


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    Structured Review

    Thermo Fisher deoxynucleoside triphosphates
    Deoxynucleoside Triphosphates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 708 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphates/product/Thermo Fisher
    Average 94 stars, based on 708 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphates - by Bioz Stars, 2020-07
    94/100 stars

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    Related Articles

    Amplification:

    Article Title: Development of a Rotavirus-Shedding Model in Rhesus Macaques, Using a Homologous Wild-Type Rotavirus of a New P Genotype
    Article Snippet: .. The reaction mixture was cooled, 2 U of RNase H in 1 μl was added, and the reaction mixture was incubated at 37°C for 20 min. PCR was carried out in a 50-μl volume containing 2.0 μl of the RT-PCR products, 5 μl of 10× amplification buffer, 1.5 μl of 10 mM deoxynucleoside triphosphates, 1.0 μl of 50 mM MgSO4 , 1.5 μl of forward and 1.5 μl of reverse primer (200 nM each), and 2.5 U of Platinum Pfx DNA polymerase (Invitrogen Life Technologies). ..

    Polymerase Chain Reaction:

    Article Title: Development of a Rotavirus-Shedding Model in Rhesus Macaques, Using a Homologous Wild-Type Rotavirus of a New P Genotype
    Article Snippet: .. The reaction mixture was cooled, 2 U of RNase H in 1 μl was added, and the reaction mixture was incubated at 37°C for 20 min. PCR was carried out in a 50-μl volume containing 2.0 μl of the RT-PCR products, 5 μl of 10× amplification buffer, 1.5 μl of 10 mM deoxynucleoside triphosphates, 1.0 μl of 50 mM MgSO4 , 1.5 μl of forward and 1.5 μl of reverse primer (200 nM each), and 2.5 U of Platinum Pfx DNA polymerase (Invitrogen Life Technologies). ..

    Article Title: Identification of Streptococcus bovis Biotype I Strains among S. bovis Clinical Isolates by PCR
    Article Snippet: .. All PCRs were performed in a Robocycler 96 machine (Stratagene) and contained, in a 50-μl total volume, 250 ng of S. bovis genomic DNA, 2.5 mM MgCl2 , 1× PCR buffer, a 200 μM concentration of each of the deoxynucleoside triphosphates, a 0.25 μM concentration of each of the forward and reverse primers, and 1 U of Taq DNA polymerase (Gibco-BRL). .. The PCR conditions for the clone 40 and 51 biotyping PCRs were as follows: 1 cycle of 94°C for 2 min, 60°C for 1 min, and 72°C for 1 min; followed by 24 cycles of 94°C for 1 min, 60°C for 1 min, and 72°C for 1 min; followed by a final cycle of 72°C for 2 min.

    Article Title: Detection and Genotyping of Varicella-Zoster Virus by TaqMan Allelic Discrimination Real-Time PCR
    Article Snippet: .. GeneAmp PCR gold buffer, deoxynucleoside triphosphates, MgCl2 , and AmpliTaq Gold DNA polymerase were purchased from Applied Biosystems (Foster City, Calif.). .. PCR products were subjected to electrophoresis in either 2% (ORF 38 and 54) or 4% (ORF 62) agarose gels and visualized by ethidium bromide staining.

    Article Title: Free-living bacterial communities associated with tubeworm (Ridgeia piscesae) aggregations in contrasting diffuse flow hydrothermal vent habitats at the Main Endeavour Field, Juan de Fuca Ridge
    Article Snippet: .. The final reaction mixture used (20 μL) contained 1 μL template DNA, 1.875 mmol/L MgCl2 , 0.8 mmol/L deoxynucleoside triphosphates, 0.25 μmol/L (each) primer, 1X PCR buffer (Invitrogen, Valencia, CA), and 1 U of Taq DNA polymerase (Invitrogen). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Development of a Rotavirus-Shedding Model in Rhesus Macaques, Using a Homologous Wild-Type Rotavirus of a New P Genotype
    Article Snippet: .. The reaction mixture was cooled, 2 U of RNase H in 1 μl was added, and the reaction mixture was incubated at 37°C for 20 min. PCR was carried out in a 50-μl volume containing 2.0 μl of the RT-PCR products, 5 μl of 10× amplification buffer, 1.5 μl of 10 mM deoxynucleoside triphosphates, 1.0 μl of 50 mM MgSO4 , 1.5 μl of forward and 1.5 μl of reverse primer (200 nM each), and 2.5 U of Platinum Pfx DNA polymerase (Invitrogen Life Technologies). ..

    Concentration Assay:

    Article Title: Identification of Streptococcus bovis Biotype I Strains among S. bovis Clinical Isolates by PCR
    Article Snippet: .. All PCRs were performed in a Robocycler 96 machine (Stratagene) and contained, in a 50-μl total volume, 250 ng of S. bovis genomic DNA, 2.5 mM MgCl2 , 1× PCR buffer, a 200 μM concentration of each of the deoxynucleoside triphosphates, a 0.25 μM concentration of each of the forward and reverse primers, and 1 U of Taq DNA polymerase (Gibco-BRL). .. The PCR conditions for the clone 40 and 51 biotyping PCRs were as follows: 1 cycle of 94°C for 2 min, 60°C for 1 min, and 72°C for 1 min; followed by 24 cycles of 94°C for 1 min, 60°C for 1 min, and 72°C for 1 min; followed by a final cycle of 72°C for 2 min.

    Incubation:

    Article Title: Development of a Rotavirus-Shedding Model in Rhesus Macaques, Using a Homologous Wild-Type Rotavirus of a New P Genotype
    Article Snippet: .. The reaction mixture was cooled, 2 U of RNase H in 1 μl was added, and the reaction mixture was incubated at 37°C for 20 min. PCR was carried out in a 50-μl volume containing 2.0 μl of the RT-PCR products, 5 μl of 10× amplification buffer, 1.5 μl of 10 mM deoxynucleoside triphosphates, 1.0 μl of 50 mM MgSO4 , 1.5 μl of forward and 1.5 μl of reverse primer (200 nM each), and 2.5 U of Platinum Pfx DNA polymerase (Invitrogen Life Technologies). ..

    Article Title: Regulation of LiaRS-Dependent Gene Expression in Bacillus subtilis: Identification of Inhibitor Proteins, Regulator Binding Sites, and Target Genes of a Conserved Cell Envelope Stress-Sensing Two-Component System
    Article Snippet: .. Following hybridization, 50 μl extension buffer (72 mM NaCl, 50 mM Tris-HCl [pH 7.9], 10 mM dithiothreitol, 20 mM MgCl2 ), deoxynucleoside triphosphates (10 mM), and 2 μl SuperScript II reverse transcriptase (Invitrogen) were added to the mixture and incubation continued at 37°C for 30 min. .. The primer extension products were precipitated with ethanol, resuspended in sequence loading buffer, and loaded onto 6% polyacrylamide sequencing gels.

    Modification:

    Article Title: Development of PCRSeqTyping—a novel molecular assay for typing of Streptococcus pneumoniae
    Article Snippet: .. The reaction mixture was modified to contain 50–100 ng of genomic DNA, 0.75 units XT-5 polymerase (3 U/μl, Merck, which is a mixture of thermo stable enzymes Taq DNA polymerase and proof-reading [PR] polymerase), 1X XT5A-Assay buffer, 1 μl deoxynucleoside triphosphates (dNTPs, 2.5 mM each [Fermentas, United States]), 1 μl forward primer (100 ng/μl), 1 μl of reverse primer mix (100 ng/μl). .. The final reaction volume was made up to 25 μl with DNase/RNase-free distilled water (Gibco, United States).

    Hybridization:

    Article Title: Regulation of LiaRS-Dependent Gene Expression in Bacillus subtilis: Identification of Inhibitor Proteins, Regulator Binding Sites, and Target Genes of a Conserved Cell Envelope Stress-Sensing Two-Component System
    Article Snippet: .. Following hybridization, 50 μl extension buffer (72 mM NaCl, 50 mM Tris-HCl [pH 7.9], 10 mM dithiothreitol, 20 mM MgCl2 ), deoxynucleoside triphosphates (10 mM), and 2 μl SuperScript II reverse transcriptase (Invitrogen) were added to the mixture and incubation continued at 37°C for 30 min. .. The primer extension products were precipitated with ethanol, resuspended in sequence loading buffer, and loaded onto 6% polyacrylamide sequencing gels.

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  • 94
    Thermo Fisher deoxynucleoside triphosphates
    Deoxynucleoside Triphosphates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 708 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphates/product/Thermo Fisher
    Average 94 stars, based on 708 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphates - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    93
    Thermo Fisher deoxynucleoside triphosphates dntps
    Deoxynucleoside Triphosphates Dntps, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphates dntps/product/Thermo Fisher
    Average 93 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphates dntps - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

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