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TaKaRa deoxynucleoside triphosphates
Deoxynucleoside Triphosphates, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxynucleoside triphosphates/product/TaKaRa
Average 93 stars, based on 26 article reviews
Price from $9.99 to $1999.99
deoxynucleoside triphosphates - by Bioz Stars, 2020-07
93/100 stars

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Polymerase Chain Reaction:

Article Title: Bacterial Communities Changes during Food Waste Spoilage
Article Snippet: .. PCR amplifications were conducted in a total reaction volume of 50 mL containing 1 μL (10 μM) each forward/reverse primer, 1 μL (approximately 30 ng/μL) genomic DNA, 4 μL (2.5 μM) deoxynucleoside triphosphates, and 0.4 μL (2U) Taq DNA polymerase (Takara, Japan). ..

Article Title: Isolation and Characterization of Methanothermobacter crinale sp. nov., a Novel Hydrogenotrophic Methanogen from the Shengli Oil Field ▿ sp. nov., a Novel Hydrogenotrophic Methanogen from the Shengli Oil Field ▿ †
Article Snippet: .. The 16S rRNA and mcrA gene fragments were amplified using primers Ar21F/1492R, Ar109f/Ar915r, and MCRf/MCRr, respectively ( , , ), reaction mixtures of 50 μl consisted of 10 to 50 ng template DNA, 5 μl 10× PCR buffer, 4 μl deoxynucleoside triphosphates (each at 2.5 mM), 3 μl MgCl2 (25 mM), 0.25 μl TaKaRa Taq (5 U/μl), and 1 μl each of the forward and reverse primers (10 μM for the 16S rRNA gene and 50 μM for the mcrA gene). .. Amplification with archaeal primers Ar21F and 1492R was carried out as follows: 94°C for 4 min, followed by 30 cycles of 94°C for 1 min, 52°C for 1 min, and 72°C for 2 min, with a final extension at 72°C for 7 min. Amplification with archaeal primers Ar109f and Ar915r was described previously ( ).

Article Title: Identification of selected respiratory pathogens in endodontic infections
Article Snippet: .. For both single step and nested format assays, amplification mixture of 50 μL contained 10 μL extracted DNA from root canal samples, 5 μL 10X PCR buffer, 0.5 μL (2.5 U) HotStar Taq DNA polymerase (Qiagen), 0.2 mmol/L concentrations of each of the 4 deoxynucleoside triphosphates (Takara, Otsu, Shiga, Japan) and a 0.5 μmol/L concentration of each (sense and antisense) primer. ..

Article Title: Bloom of Filamentous Bacteria in a Mesotrophic Lake: Identity and Potential Controlling Mechanism
Article Snippet: .. For PCR, 5 μl of bovine serum albumin (stock concentration, 3 mg ml−1 ), 5 μl of 10× PCR buffer, 2 μl of deoxynucleoside triphosphates (stock concentration, 2.5 mM), 0.5 μl of the general bacterial primers GM3F and GM4R ( ) (stock concentration, 15 μM), and 0.25 μl of TaKaRa- Taq DNA polymerase (stock concentration, 5 U per reaction mixture; TaKaRa BIO Inc., Shiga, Japan) were adjusted to a final volume of 48 μl with sterile water. ..

Concentration Assay:

Article Title: Identification of selected respiratory pathogens in endodontic infections
Article Snippet: .. For both single step and nested format assays, amplification mixture of 50 μL contained 10 μL extracted DNA from root canal samples, 5 μL 10X PCR buffer, 0.5 μL (2.5 U) HotStar Taq DNA polymerase (Qiagen), 0.2 mmol/L concentrations of each of the 4 deoxynucleoside triphosphates (Takara, Otsu, Shiga, Japan) and a 0.5 μmol/L concentration of each (sense and antisense) primer. ..

Article Title: Bloom of Filamentous Bacteria in a Mesotrophic Lake: Identity and Potential Controlling Mechanism
Article Snippet: .. For PCR, 5 μl of bovine serum albumin (stock concentration, 3 mg ml−1 ), 5 μl of 10× PCR buffer, 2 μl of deoxynucleoside triphosphates (stock concentration, 2.5 mM), 0.5 μl of the general bacterial primers GM3F and GM4R ( ) (stock concentration, 15 μM), and 0.25 μl of TaKaRa- Taq DNA polymerase (stock concentration, 5 U per reaction mixture; TaKaRa BIO Inc., Shiga, Japan) were adjusted to a final volume of 48 μl with sterile water. ..

Amplification:

Article Title: Resolving the cause of recurrent Plasmodium vivax malaria probabilistically
Article Snippet: .. All amplification reactions were performed in a total volume of 10 μL and in the presence of 10 mmol/L Tris-HCl (pH 8.3), 50 mmol/L KCl, 250 nmol/L of each oligonucleotide primer, 2.5 mmol/L MgCl2 , 125 μmol/L of each of the four deoxynucleoside triphosphates, and 0.4 U of TaKaRa polymerase (TaKaRa BIO). .. Primary amplification reactions were initiated with 2 μL of the template genomic DNA prepared from the blood samples, and 1 μL of the product of these reactions was used to initiate the secondary amplification reactions.

Article Title: Isolation and Characterization of Methanothermobacter crinale sp. nov., a Novel Hydrogenotrophic Methanogen from the Shengli Oil Field ▿ sp. nov., a Novel Hydrogenotrophic Methanogen from the Shengli Oil Field ▿ †
Article Snippet: .. The 16S rRNA and mcrA gene fragments were amplified using primers Ar21F/1492R, Ar109f/Ar915r, and MCRf/MCRr, respectively ( , , ), reaction mixtures of 50 μl consisted of 10 to 50 ng template DNA, 5 μl 10× PCR buffer, 4 μl deoxynucleoside triphosphates (each at 2.5 mM), 3 μl MgCl2 (25 mM), 0.25 μl TaKaRa Taq (5 U/μl), and 1 μl each of the forward and reverse primers (10 μM for the 16S rRNA gene and 50 μM for the mcrA gene). .. Amplification with archaeal primers Ar21F and 1492R was carried out as follows: 94°C for 4 min, followed by 30 cycles of 94°C for 1 min, 52°C for 1 min, and 72°C for 2 min, with a final extension at 72°C for 7 min. Amplification with archaeal primers Ar109f and Ar915r was described previously ( ).

Article Title: Identification of selected respiratory pathogens in endodontic infections
Article Snippet: .. For both single step and nested format assays, amplification mixture of 50 μL contained 10 μL extracted DNA from root canal samples, 5 μL 10X PCR buffer, 0.5 μL (2.5 U) HotStar Taq DNA polymerase (Qiagen), 0.2 mmol/L concentrations of each of the 4 deoxynucleoside triphosphates (Takara, Otsu, Shiga, Japan) and a 0.5 μmol/L concentration of each (sense and antisense) primer. ..

Plasmid Preparation:

Article Title: Targeted Nuclear Import of Open Reading Frame 1 Protein Is Required for In Vivo Retrotransposition of a Telomere-Specific Non-Long Terminal Repeat Retrotransposon, SART1
Article Snippet: .. The Bgl II site of the plasmid was removed by Bgl II digestion and T4 DNA polymerase (TaKaRa) treatment in the presence of deoxynucleoside triphosphates (TaKaRa). .. Next, to make a vector adapted for GFP fusion at the C terminus, we introduced a Bgl II site instead of the stop codon (TAA) of the GFP-coding region in the above plasmid.

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    TaKaRa deoxynucleoside triphosphates
    Deoxynucleoside Triphosphates, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphates/product/TaKaRa
    Average 93 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphates - by Bioz Stars, 2020-07
    93/100 stars
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