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Roche deoxynucleoside triphosphates
Deoxynucleoside Triphosphates, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxynucleoside triphosphates/product/Roche
Average 92 stars, based on 131 article reviews
Price from $9.99 to $1999.99
deoxynucleoside triphosphates - by Bioz Stars, 2020-07
92/100 stars

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Amplification:

Article Title: Use of a Novel Panel of Nine Short Tandem Repeats for Exact and High-Resolution Fingerprinting of Aspergillus fumigatus Isolates
Article Snippet: .. Each PCR mixture contained 1 μM of all amplification primers, 0.2 mM deoxynucleoside triphosphates, 1 U of FastStart Taq DNA polymerase (Roche Diagnostics), and 1 ng of genomic DNA in 1× reaction buffer. ..

Isolation:

Article Title: The K5 Capsule of Escherichia coli Strain Nissle 1917 Is Important in Mediating Interactions with Intestinal Epithelial Cells and Chemokine Induction ▿
Article Snippet: .. Two micrograms of each isolated RNA then was reverse transcribed for 60 min at 42°C in 25-μl assays containing 25 pM oligo(dT) primer, 12.5 mM deoxynucleoside triphosphates, 40 U avian myeloblastosis virus reverse transcriptase (Roche Diagnostics, Mannheim, Germany), and 20 U of RNase inhibitor (Roche Diagnostics, Mannheim, Germany). .. For quantitative real-time PCR, equivalent amounts of cDNAs (2 μl) and 300 nM each of the corresponding forward and reverse primers were used with a SYBR green PCR kit from Eurogentic.

Purification:

Article Title: Novel 5-Flucytosine-Resistant Clade of Candida dubliniensis from Saudi Arabia and Egypt Identified by Cd25 Fingerprinting
Article Snippet: .. Reaction mixtures contained 100 ng of purified template DNA, 1× Expand High Fidelity buffer (Roche, Lewes, East Sussex, United Kingdom), deoxynucleoside triphosphates at concentrations of 0.2 mM each, 2.5 U of Expand High Fidelity PCR system enzyme mix (Roche), and 0.2 μM concentrations of either FCY1F1/FCY1R1 or FUR1F1/FUR1R1. ..

Concentration Assay:

Article Title: Polysaccharide Capsule Composition of Pneumococcal Serotype 19A Subtypes Is Unaltered among Subtypes and Independent of the Nutritional Environment
Article Snippet: .. The reaction mixture contained 2.5 μl of FastStart Taq reaction buffer without MgCl2 , 2.7 μl of 25 mM MgCl2 stock solution, 4 μl of 1.25 mM deoxynucleoside triphosphates (0.2 mM final concentration), 0.2 μl (1 U) of FastStart Taq polymerase (all from Roche Molecular Biochemicals, Rotkreuz, Switzerland), and 0.25 μl of each primer (100 μM stock resulting in 1 μM final concentration, Microsynth AG, Balgach, Switzerland) in a total volume of 25 μl. .. PCR products were visualized on 1% agarose gels.

Random Hexamer Labeling:

Article Title: Genetic Diversity of Norovirus and Sapovirus in Hospitalized Infants with Sporadic Cases of Acute Gastroenteritis in Chiang Mai, Thailand
Article Snippet: .. Reverse transcription was carried out in a final volume of 20 μl with 10 μl of RNA in 50 pmol of random hexamer (Takara), 1× Superscript II reverse transcription buffer (Invitrogen), 10 mM dithiothreitol (Invitrogen), 0.4 mM each of the four deoxynucleoside triphosphates (Roche), 1 U of RNase inhibitor (Toyobo), and 10 U of Superscript II reverse transcriptase (Invitrogen). .. The norovirus PCR primers were selected from three reports that described detection of a broad range of strains ( , , ).

Polymerase Chain Reaction:

Article Title: Use of a Novel Panel of Nine Short Tandem Repeats for Exact and High-Resolution Fingerprinting of Aspergillus fumigatus Isolates
Article Snippet: .. Each PCR mixture contained 1 μM of all amplification primers, 0.2 mM deoxynucleoside triphosphates, 1 U of FastStart Taq DNA polymerase (Roche Diagnostics), and 1 ng of genomic DNA in 1× reaction buffer. ..

Article Title: Genetic characterization and improved genotyping of the dysferlin-deficient mouse strain Dysftm1Kcam
Article Snippet: .. The PCR was performed in 20 μl containing the following: 4 μl Expand Long Range buffer with MgCl2 (Roche 04829034001), 0.5 μl of 10 mM deoxynucleoside triphosphates from the Expand Long Range dNTPack (Roche 04829034001), 0.25 μl Expand Long Range Enzyme Mix (Roche 04829034001), 0.5 μl of each wild-type specific primer (10 μM), 0.25 μl of each neo primer (10 μM), and 2 μl DMSO (Roche 04829034001). .. Thirty-five cycles of denaturation (92 °C, 10 s), annealing (56 °C, 15 s), and primer extension (68 °C, 105 s) were performed, followed by a single extension step (68 °C, 2 min), in a thermocycler (StepOnePlus, Applied Biosystems).

Article Title: A single gene for lycopene cyclase, phytoene synthase, and regulation of carotene biosynthesis in Phycomyces
Article Snippet: .. The PCR mixtures contained about 5 ng genomic DNA, 0.2 mM of each of the four deoxynucleoside triphosphates, 1 μM of each primer, and 0.5 μl of the DNA polymerase mixture Expand High Fidelity (Roche) in a final volume of 50 μl. .. After overlaying them with light mineral oil, they were incubated once at 95°C for 3 min, 35 times at 95°C for 1 min, 55°C for 1 min, and 68°C for 3 min, and once at 68°C for 10 min in a GeneAmp 2400 programmable thermocycler (Perkin–Elmer/Cetus).

Article Title: Novel 5-Flucytosine-Resistant Clade of Candida dubliniensis from Saudi Arabia and Egypt Identified by Cd25 Fingerprinting
Article Snippet: .. Reaction mixtures contained 100 ng of purified template DNA, 1× Expand High Fidelity buffer (Roche, Lewes, East Sussex, United Kingdom), deoxynucleoside triphosphates at concentrations of 0.2 mM each, 2.5 U of Expand High Fidelity PCR system enzyme mix (Roche), and 0.2 μM concentrations of either FCY1F1/FCY1R1 or FUR1F1/FUR1R1. ..

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  • 92
    Roche deoxynucleoside triphosphates
    Deoxynucleoside Triphosphates, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphates/product/Roche
    Average 92 stars, based on 131 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphates - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    88
    Roche rt cocktail
    Rt Cocktail, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rt cocktail/product/Roche
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rt cocktail - by Bioz Stars, 2020-07
    88/100 stars
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