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Promega deoxynucleoside triphosphates
Deoxynucleoside Triphosphates, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 342 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxynucleoside triphosphates/product/Promega
Average 92 stars, based on 342 article reviews
Price from $9.99 to $1999.99
deoxynucleoside triphosphates - by Bioz Stars, 2020-07
92/100 stars

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Amplification:

Article Title: Yeast Diversity and Persistence in Botrytis-Affected Wine Fermentations
Article Snippet: .. DNA was amplified in a 50-μl final volume containing 10 mM Tris-HCl (pH 8), 50 mM KCl, 1.5 mM MgCl2 , 0.2 mM deoxynucleoside triphosphates, 0.2 μM primers, 1.25 U of Taq polymerase (Promega), and 5 μl of extracted Candida sp. strain EJ1 DNA (about 10 to 50 ng of total DNA). .. The PCR cycle parameters were 30 cycles of denaturation at 95°C for 1 min, annealing at 60°C for 45 s, and extension at 72°C for 1 min. An initial denaturation at 95°C for 5 min and a final extension at 72°C for 7 min were also used.

Article Title: Immunogenicity of Seven New Recombinant Yellow Fever Viruses 17D Expressing Fragments of SIVmac239 Gag, Nef, and Vif in Indian Rhesus Macaques
Article Snippet: .. The final reaction mixtures (100-µl total volume) contained 0.2 mM (each) deoxynucleoside triphosphates, 3.5 mM MgS04 , 750 ng of random hexamer primers (Promega, Madison, WI), 4.0 µl of SuperScript III reverse transcriptase and Platinum Taq DNA polymerase in a single enzyme mix, 600 nM (each) amplification primers (forward [YF-17D 10188], 5′-GCGGATCACTGATTGGAATGAC-3′ , and reverse [YF-17D 10264], 5′-CGTTCGGATACGATGGATGACTA-3′ ), and 100 nM probe [6Fam]5′-AATAGGGCCACCTGGGCCTCCC-3′[TamraQ]. ..

Synthesized:

Article Title: CCAAT/Enhancer Binding Protein ? Binds to the Epstein-Barr Virus (EBV) ZTA Protein through Oligomeric Interactions and Contributes to Cooperative Transcriptional Activation of the ZTA Promoter through Direct Binding to the ZII and ZIIIB Motifs during Induction of the EBV Lytic Cycle
Article Snippet: .. The synthesized cDNAs were used as templates for ZTA PCRs with primers LGH2617 (5′-ACATCTGCTTCAACAGGAGG-3′) and LGH2618 (5′-AGCAGACATTGGTGTTCCAC-3′); the PCR mixtures contained 2 μl of cDNA template, 0.25 μg of each primer, 3 μl of 25 mM MgCl2 , 4 μl of 2.5 mM deoxynucleoside triphosphates, 3.5 μl of dimethyl sulfoxide, 2.5 U of Taq DNA polymerase (Promega), 5 μl of 10× buffer (Promega), and H2 O to a final volume of 50 μl. ..

Incubation:

Article Title: Prevalence of Human Parechovirus in The Netherlands in 2000 to 2007 ▿
Article Snippet: .. The samples were subsequently chilled on ice for 2 min and incubated at 42°C for 60 min together with 1.5 μl of 10× PCR buffer (pH 8.3) (100 mM Tris-HCl, 500 mM KCl), 1.8 μl of 25 mM MgCl2 , 1.5 μl of 10 mM deoxynucleoside triphosphates, 0.5 μl of 10-U/μl avian myeloblastosis virus reverse transcriptase (Promega, Leiden, The Netherlands), and 0.7 μl of H2 O. .. PCR amplification of the 5′ UTR and VP1/2A region was performed on a LightCycler instrument.

Polymerase Chain Reaction:

Article Title: Five-Year Follow-Up Study of Mother-to-Child Transmission of Helicobacter pylori Infection Detected by a Random Amplified Polymorphic DNA Fingerprinting Method
Article Snippet: .. PCR was carried out in a final volume of 50 μl containing 20 ng template DNA, 20 pmol primer, 1.5 mM MgCl2 , 1.25 U of Taq DNA polymerase (Promega, WI, USA), 0.1 mM deoxynucleoside triphosphates (Promega) in 19 mM Tris-HCl (pH 8.3) containing 50 mM KCl and Triton X-100 (1%, vol/vol). .. For the analysis of the RAPD fingerprinting, random primers from DNA Oligomer ( ) set A-1 (NIPPON GENE, Toyama, Japan) were screened for amplification of H. pylori DNA.

Article Title: Kodamaea ohmeri Isolates from Patients in a University Hospital: Identification, Antifungal Susceptibility, and Pulsed-Field Gel Electrophoresis Analysis ▿
Article Snippet: .. The PCR was performed with a total reaction mixture volume of 50 μl consisting of 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , 0.2 mM deoxynucleoside triphosphates, 1.2 U of GoTaq DNA polymerase (Promega Corporation, Madison, WI), 0.4 μM (each) ITS2 region primers (ITS3/ITS4), and 2 μl (1 to 5 ng) of DNA template. .. PCR was carried out using the following conditions: initial denaturation at 94°C for 5 min; 30 cycles of denaturation (94°C for 30 s), annealing (55°C for 30 s), and extension (72°C for 30 s); and a final extension step at 72°C for 5 min.

Article Title: Prevalence of Human Parechovirus in The Netherlands in 2000 to 2007 ▿
Article Snippet: .. The samples were subsequently chilled on ice for 2 min and incubated at 42°C for 60 min together with 1.5 μl of 10× PCR buffer (pH 8.3) (100 mM Tris-HCl, 500 mM KCl), 1.8 μl of 25 mM MgCl2 , 1.5 μl of 10 mM deoxynucleoside triphosphates, 0.5 μl of 10-U/μl avian myeloblastosis virus reverse transcriptase (Promega, Leiden, The Netherlands), and 0.7 μl of H2 O. .. PCR amplification of the 5′ UTR and VP1/2A region was performed on a LightCycler instrument.

Article Title: CCAAT/Enhancer Binding Protein ? Binds to the Epstein-Barr Virus (EBV) ZTA Protein through Oligomeric Interactions and Contributes to Cooperative Transcriptional Activation of the ZTA Promoter through Direct Binding to the ZII and ZIIIB Motifs during Induction of the EBV Lytic Cycle
Article Snippet: .. The synthesized cDNAs were used as templates for ZTA PCRs with primers LGH2617 (5′-ACATCTGCTTCAACAGGAGG-3′) and LGH2618 (5′-AGCAGACATTGGTGTTCCAC-3′); the PCR mixtures contained 2 μl of cDNA template, 0.25 μg of each primer, 3 μl of 25 mM MgCl2 , 4 μl of 2.5 mM deoxynucleoside triphosphates, 3.5 μl of dimethyl sulfoxide, 2.5 U of Taq DNA polymerase (Promega), 5 μl of 10× buffer (Promega), and H2 O to a final volume of 50 μl. ..

Random Hexamer Labeling:

Article Title: Immunogenicity of Seven New Recombinant Yellow Fever Viruses 17D Expressing Fragments of SIVmac239 Gag, Nef, and Vif in Indian Rhesus Macaques
Article Snippet: .. The final reaction mixtures (100-µl total volume) contained 0.2 mM (each) deoxynucleoside triphosphates, 3.5 mM MgS04 , 750 ng of random hexamer primers (Promega, Madison, WI), 4.0 µl of SuperScript III reverse transcriptase and Platinum Taq DNA polymerase in a single enzyme mix, 600 nM (each) amplification primers (forward [YF-17D 10188], 5′-GCGGATCACTGATTGGAATGAC-3′ , and reverse [YF-17D 10264], 5′-CGTTCGGATACGATGGATGACTA-3′ ), and 100 nM probe [6Fam]5′-AATAGGGCCACCTGGGCCTCCC-3′[TamraQ]. ..

Reverse Transcription Polymerase Chain Reaction:

Article Title: Nested Restriction Site-Specific PCR To Detect and Type Hepatitis C Virus (HCV): a Rapid Method To Distinguish HCV Subtype 1b from Other Genotypes
Article Snippet: .. The RT-PCR assay was performed with 5 μl of the extracted RNA added to 20 μl of an RT-PCR mixture consisting of 50 mM potassium chloride, 10 mM Tris-HCl (pH 8.5), 3.0 mM magnesium chloride, 0.01% gelatin, 200 μM concentrations of each of the four deoxynucleoside triphosphates, 10 mM dithiothreitol, 0.25 μM concentrations each of the primers Bukh-E1 and RSS-E2, 0.63 U of avian myeloblastosis virus reverse transcriptase (Promega), 10 U of RNAsin (Promega), and 0.63 U of Taq DNA polymerase (AmpliTaq; Perkin-Elmer Corp., Foster City, Calif.). ..

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  • 92
    Promega deoxynucleoside triphosphates
    Deoxynucleoside Triphosphates, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 342 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphates/product/Promega
    Average 92 stars, based on 342 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphates - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    88
    Promega buffer conditions
    Buffer Conditions, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/buffer conditions/product/Promega
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    buffer conditions - by Bioz Stars, 2020-07
    88/100 stars
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    85
    Promega deoxynucleoside triphosphates pharmacia
    Deoxynucleoside Triphosphates Pharmacia, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphates pharmacia/product/Promega
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphates pharmacia - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

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