deoxynucleoside triphosphates  (Millipore)


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    Structured Review

    Millipore deoxynucleoside triphosphates
    Deoxynucleoside Triphosphates, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphates/product/Millipore
    Average 93 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphates - by Bioz Stars, 2020-07
    93/100 stars

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    Amplification:

    Article Title: Human Immunodeficiency Virus Type 1-Induced Macrophage Gene Expression Includes the p21 Gene, a Target for Viral Regulation
    Article Snippet: .. PCRs were performed with 1.5 mM MgCl2 , 200 μM deoxynucleoside triphosphates, a 0.6 μM concentration of each primer, and 1 U of Taq polymerase (Sigma). cDNAs were amplified for 25 cycles with the following settings: 94°C for 30 s, 55°C for 30 s, and 72°C for 30 s. .. For analyses of newly synthesized viral DNAs , DNase-treated HIV-1BaL (200 μl; 104 /ml) was added for 2 h to macrophages (6 × 106 cells/well) that had been pretreated with a p21 small interfering RNA (siRNA) or a control siRNA.

    Mutagenesis:

    Article Title: Development and Evaluation of a Sensitive and Specific Assay for Diagnosis of Human Toxocariasis by Use of Three Recombinant Antigens (TES-26, TES-30USM, and TES-120) ▿
    Article Snippet: .. Plasmid DNA template (4 μl) was added to a PCR mixture consisting of 5 μl of 10× mutagenesis buffer, 2 μl each of 1 μM forward and reverse mutagenic primers, 1 μl of 10 mM deoxynucleoside triphosphates, 1 μl of Pfu DNA polymerase (2.5 U/μl), and water (DNase and RNase free; Sigma) to a final volume of 50 μl. ..

    Concentration Assay:

    Article Title: Human Immunodeficiency Virus Type 1-Induced Macrophage Gene Expression Includes the p21 Gene, a Target for Viral Regulation
    Article Snippet: .. PCRs were performed with 1.5 mM MgCl2 , 200 μM deoxynucleoside triphosphates, a 0.6 μM concentration of each primer, and 1 U of Taq polymerase (Sigma). cDNAs were amplified for 25 cycles with the following settings: 94°C for 30 s, 55°C for 30 s, and 72°C for 30 s. .. For analyses of newly synthesized viral DNAs , DNase-treated HIV-1BaL (200 μl; 104 /ml) was added for 2 h to macrophages (6 × 106 cells/well) that had been pretreated with a p21 small interfering RNA (siRNA) or a control siRNA.

    other:

    Article Title: Loop-Mediated Isothermal Amplification Assay for Detection of Haemophilus influenzae Type b in Cerebrospinal Fluid ▿
    Article Snippet: The reaction mixture (25 μl) contained 1.6 μM concentrations (each) of FIP and BIP, 0.2 μM concentrations (each) of F3 and B3, 0.4 μM LF, 8 U of Bst DNA polymerase large fragment (New England Biolabs, Ipswich, MA), 1.4 mM deoxynucleoside triphosphates, 0.8 M betaine (Sigma, St. Louis, MO), 20 mM Tris-HCl (pH 8.8), 10 mM KCl, 10 mM (NH4 )2 SO4 , 8 mM MgSO4 , 0.1% Tween 20, and template DNA (2 μl).

    Polymerase Chain Reaction:

    Article Title: Development and Evaluation of a Sensitive and Specific Assay for Diagnosis of Human Toxocariasis by Use of Three Recombinant Antigens (TES-26, TES-30USM, and TES-120) ▿
    Article Snippet: .. Plasmid DNA template (4 μl) was added to a PCR mixture consisting of 5 μl of 10× mutagenesis buffer, 2 μl each of 1 μM forward and reverse mutagenic primers, 1 μl of 10 mM deoxynucleoside triphosphates, 1 μl of Pfu DNA polymerase (2.5 U/μl), and water (DNase and RNase free; Sigma) to a final volume of 50 μl. ..

    Article Title: Insertion or Deletion of the Cheo Box Modifies Radiation Inducibility of Clostridium Promoters
    Article Snippet: .. Five microliters of the RT mixture was added to a mixture containing 8.5 μl of reverse primer (10 pmol/μl), 7 μl of forward primer (10 pmol/μl), 2.5 μl of deoxynucleoside triphosphates (5 mM each), 0.5 U of JumpStart Taq DNA polymerase (Sigma Chemical Co., St. Louis, Mo.), and 4.5 μl of 10× PCR buffer in a total volume of 50 μl. .. After 40 PCR cycles (10 min at 95°C, 30 s at 95°C, 2 min at 40°C, 30 s at 72°C, and 5 min at 72°C), 1-μl aliquots were run on 1% agarose gels.

    Article Title: Persisting PET-CT lesion activity and M. tuberculosis mRNA after pulmonary tuberculosis cure
    Article Snippet: .. The 10 μl PCR reaction mixture consisted of 1X PCR buffer, 250 μM deoxynucleoside triphosphates, 4 mM MgCl2 , 0.5 μM each primer, 4ng/μl molecular beacon and 0.03U/μl Jumpstart Taq polymerase (Sigma-Aldrich). ..

    Plasmid Preparation:

    Article Title: Development and Evaluation of a Sensitive and Specific Assay for Diagnosis of Human Toxocariasis by Use of Three Recombinant Antigens (TES-26, TES-30USM, and TES-120) ▿
    Article Snippet: .. Plasmid DNA template (4 μl) was added to a PCR mixture consisting of 5 μl of 10× mutagenesis buffer, 2 μl each of 1 μM forward and reverse mutagenic primers, 1 μl of 10 mM deoxynucleoside triphosphates, 1 μl of Pfu DNA polymerase (2.5 U/μl), and water (DNase and RNase free; Sigma) to a final volume of 50 μl. ..

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    Millipore unincorporated amino allyl deoxynucleoside triphosphates
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    Millipore deoxynucleoside triphosphates dntps
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    https://www.bioz.com/result/deoxynucleoside triphosphates dntps/product/Millipore
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    Millipore biotinylated deoxynucleoside triphosphates
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    https://www.bioz.com/result/biotinylated deoxynucleoside triphosphates/product/Millipore
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