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Meridian Life Science deoxynucleoside triphosphates
Deoxynucleoside Triphosphates, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxynucleoside triphosphates/product/Meridian Life Science
Average 92 stars, based on 4 article reviews
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deoxynucleoside triphosphates - by Bioz Stars, 2020-07
92/100 stars

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Polymerase Chain Reaction:

Article Title: Contrasting Responses to Nutrient Enrichment of Prokaryotic Communities Collected from Deep Sea Sites in the Southern Ocean
Article Snippet: .. The reactions (50 µL) were prepared on ice and contained 250 µM each of the deoxynucleoside triphosphates, 1× PCR buffer, 7.5 picomoles of each oligonucleotide primer, 2.0–2.5 mM MgCl2 and 1.5 units of Taq DNA polymerase (Bioline, London, UK) in 18 MΩ analytical grade water (Sigma, Dorset, UK). .. PCR was performed in a G-Storm GS1 thermal cycler (Labtech, East Sussex, UK) using the following programs: Bacterial 16S rRNA gene: Denaturation for 4 min at 94 °C, 10 cycles of denaturation (30 s at 94 °C), annealing (1 min at 58 °C) and extension (1 min at 72 °C), followed by 22 cycles of denaturation (30 s at 92 °C), annealing (1 min at 58 °C) and extension (1 min at 72 °C), with a final extension for 10 min at 72 °C.

Article Title: Response of Methanogens in Arctic Sediments to Temperature and Methanogenic Substrate Availability
Article Snippet: .. PCR reactions (50 μl) contained extracted DNA (~5–30 ng), primers (10 pmol of each), deoxynucleoside triphosphates (10 mM each), MgCl2 (1.5 mM), buffer (5 μl of 10 x stock) and Taq DNA polymerase (4U, Bioline, London, UK), and were carried out using an automated thermal cycler (G-storm GS1; GRI Ltd, Essex, UK) or a PCR Sprint (Hybaid Ltd, Hampshire, UK). .. The first PCR reaction comprised an initial denaturation step (94°C for 3 minutes), 30 cycles of denaturation (94°C for 1 minute), annealing (40°C for 1 minute) and extension (72°C for 1 minute) followed by a final extension of 72°C for 10 minutes.

Article Title: No Evidence for a Parent-of-Origin Specific Differentially Methylated Region Linked to RASGRF1
Article Snippet: .. All amplicons were generated in a 50-μl reaction containing 2 μl of bisulfite-treated DNA, 0.2 μM each of the forward and reverse PCR primers (primers are listed in Table ) and 0.02 μM of the universal primer, 0.125 mM deoxynucleoside triphosphates (Bioline, London, UK), 2.0 mM MgCl2 , 1× Buffer (Applied Biosystems, NJ, USA), 1 M Betaine (Sigma-Aldrich, Seelze, Germany), and 1 U of AmpliTaq Gold (Applied Biosystems, NJ, USA). .. For IG-DMR, a nested PCR was performed, first with the outer primer set and the same reaction mix as above.

Article Title: Culture-Independent Analysis of Indomethacin-Induced Alterations in the Rat Gastrointestinal Microbiota
Article Snippet: .. Thirty-microliter PCR mixtures contained 1× PCR buffer (Bioline USA Inc., Randolph, MA), 2.5 mM MgCl2 , deoxynucleoside triphosphates (0.21 mM each), 25 ng of each primer, 1 unit of Taq DNA polymerase (Bioline USA Inc., Randolph, MA), and ∼2 mg (2 μl) of genomic DNA lysate. .. The PCR mixtures were amplified using touchdown cycling ( ).

Article Title: In Vitro Antifungal Susceptibilities and Amplified Fragment Length Polymorphism Genotyping of a Worldwide Collection of 350 Clinical, Veterinary, and Environmental Cryptococcus gattii Isolates ▿
Article Snippet: .. Briefly, 1 μl genomic DNA (100 ng/μl) was added to 24 μl PCR mixture consisting of 17.8 μl double-distilled H2 O, 0.75 μl MgCl2 (50 mM; Bioline, London, United Kingdom), 2.5 μl 10× PCR buffer (Bioline), 1.9 μl deoxynucleoside triphosphates (1 mM; Bioline), 0.1 μl Taq DNA polymerase (5 U/μl; Bioline), and 0.5 μl of the forward and reverse primers (Biolegio, Nijmegen, Netherlands) for either the STE12 α allele (forward primer STE12α-F809 [5′-TTGACCTTTTRTTCCGCAATG-3′] and reverse primer STE12α-R1607 [5′-TTTCTTCTCCCCTGTTTATAGGC-3′]) or the STE12 a allele (forward primer STE12 a -F537 [5′-GTTCTTTGGAATGGCTTATTTCATAT-3′] and reverse primer STE12 a -R1299 [5′-GMCTTGCGTGGATCATATCTA-3′]). ..

Article Title: Detection of Saxitoxin-Producing Cyanobacteria and Anabaena circinalis in Environmental Water Blooms by Quantitative PCR ▿ in Environmental Water Blooms by Quantitative PCR ▿ †
Article Snippet: .. PCR was performed using 0.2 U of Taq DNA polymerase (Bioline, Australia) in a 20-μl reaction mix containing 2.5 mM MgCl2 , PCR buffer (Bioline), 0.2 mM deoxynucleoside triphosphates (dNTPs; Bioline), 10 pmol of each of the 16S rRNA gene primer (Sigma-Genosys), and 1 ng of template DNA. .. Thermal cycling was performed in a GeneAmp PCR system 2400 thermocycler (PerkinElmer Corporation).

Generated:

Article Title: No Evidence for a Parent-of-Origin Specific Differentially Methylated Region Linked to RASGRF1
Article Snippet: .. All amplicons were generated in a 50-μl reaction containing 2 μl of bisulfite-treated DNA, 0.2 μM each of the forward and reverse PCR primers (primers are listed in Table ) and 0.02 μM of the universal primer, 0.125 mM deoxynucleoside triphosphates (Bioline, London, UK), 2.0 mM MgCl2 , 1× Buffer (Applied Biosystems, NJ, USA), 1 M Betaine (Sigma-Aldrich, Seelze, Germany), and 1 U of AmpliTaq Gold (Applied Biosystems, NJ, USA). .. For IG-DMR, a nested PCR was performed, first with the outer primer set and the same reaction mix as above.