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GE Healthcare deoxynucleoside triphosphates
Deoxynucleoside Triphosphates, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxynucleoside triphosphates/product/GE Healthcare
Average 92 stars, based on 108 article reviews
Price from $9.99 to $1999.99
deoxynucleoside triphosphates - by Bioz Stars, 2020-07
92/100 stars

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Amplification:

Article Title: Comparative Evaluation of Three Different Genotyping Methods for Investigation of Nosocomial Outbreaks of Legionnaires' Disease in Hospitals
Article Snippet: .. The amplification reaction was performed in a final volume of 25 μl containing 1 μl of the diluted DNA, 75 ng of the Cy-5 fluorescently labeled Pst I-G primer (5′-GAC TGC GTA CAT GCA GG), 0.2 mM deoxynucleoside triphosphates (both from Amersham Pharmacia Biotech), 5 mM MgCl2 , 10 mM Tris-HCl (pH 9.0), 50 mM KCl, and 1 U of Taq DNA polymerase (Boehringer Mannheim). .. The PCR comprised 33 cycles of 60 s at 94°C, 60 s at 60°C, and 150 s at 72°C and a final extension step at 72°C for 5 min.

Isolation:

Article Title: Identification of a Disulfide Isomerase Protein of Leishmania major as a Putative Virulence Factor
Article Snippet: .. Poly(A)+ mRNA was purified from total RNA by using a poly(A)+ RNA isolation kit (Amersham-Pharmacia Biotech, Glylada, Greece) according to the manufacturer's recommendations. mRNA (200 ng) was used to synthesize first-strand cDNAs in a 20-μl reaction mix containing 1 μM specific anchored primer oligo(dT)11 MN (where M is A, C, or G and N is A, C, G, or T) (Genset, Paris, France), 1× first-strand buffer (Gibco-BRL), 5 μM deoxynucleoside triphosphates (Amersham-Pharmacia), 10 U of RNasin (Promega, Charbonnières, France), and 200 U of reverse transcriptase (Gibco-BRL). .. The cDNAs were PCR amplified using a combination of the 12 specific anchored primers and 10 arbitrary 5′ primers (Table ).

Labeling:

Article Title: Comparative Evaluation of Three Different Genotyping Methods for Investigation of Nosocomial Outbreaks of Legionnaires' Disease in Hospitals
Article Snippet: .. The amplification reaction was performed in a final volume of 25 μl containing 1 μl of the diluted DNA, 75 ng of the Cy-5 fluorescently labeled Pst I-G primer (5′-GAC TGC GTA CAT GCA GG), 0.2 mM deoxynucleoside triphosphates (both from Amersham Pharmacia Biotech), 5 mM MgCl2 , 10 mM Tris-HCl (pH 9.0), 50 mM KCl, and 1 U of Taq DNA polymerase (Boehringer Mannheim). .. The PCR comprised 33 cycles of 60 s at 94°C, 60 s at 60°C, and 150 s at 72°C and a final extension step at 72°C for 5 min.

Purification:

Article Title: Identification of a Disulfide Isomerase Protein of Leishmania major as a Putative Virulence Factor
Article Snippet: .. Poly(A)+ mRNA was purified from total RNA by using a poly(A)+ RNA isolation kit (Amersham-Pharmacia Biotech, Glylada, Greece) according to the manufacturer's recommendations. mRNA (200 ng) was used to synthesize first-strand cDNAs in a 20-μl reaction mix containing 1 μM specific anchored primer oligo(dT)11 MN (where M is A, C, or G and N is A, C, G, or T) (Genset, Paris, France), 1× first-strand buffer (Gibco-BRL), 5 μM deoxynucleoside triphosphates (Amersham-Pharmacia), 10 U of RNasin (Promega, Charbonnières, France), and 200 U of reverse transcriptase (Gibco-BRL). .. The cDNAs were PCR amplified using a combination of the 12 specific anchored primers and 10 arbitrary 5′ primers (Table ).

Concentration Assay:

Article Title: CDC25A Phosphatase Is a Target of E2F and Is Required for Efficient E2F-Induced S Phase
Article Snippet: .. In addition, a PCR sample contained deoxynucleoside triphosphates 50 μM each, buffer with MgCl2 (1.5 mM final concentration), primers (0.2 μM each), [32 P]dCTP (0.1 μl of 3,000 Ci/mM; Amersham Life Science), Taq polymerase (1 U; Perkin-Elmer), and water (to a final volume of 30 μl). .. To perform semiquantitative PCR, for each couple of primers we determined on cDNA from cycling Rat1 RNA (at the best annealing temperature) how many cycles were required to detect a clear signal in the linear range.

Article Title: High-Level Expression of AmpC ?-Lactamase Due to Insertion of Nucleotides between -10 and -35 Promoter Sequences in Escherichia coli Clinical Isolates: Cases Not Responsive to Extended-Spectrum-Cephalosporin Treatment
Article Snippet: .. The extension reaction was performed at 42°C for 1 h after the addition of sodium pyrophosphate (final concentration, 4 mM; Merck & Co., Inc., Rahway, N.J.), deoxynucleoside triphosphates (1 mM; Amersham-Pharmacia Biotech Inc.), and 30 U of avian myeloblastosis virus reverse transcriptase (Promega). .. The marker was generated by PCR with the same primer used for extension in the sequencing reaction (Sequenase [version 2.0] DNA sequencing kit; United States Biochemical Corp., Cleveland, Ohio) of a complete ampC gene (bp 10180 to 5316 of the sequence with GenBank accession no. AE000487 ) cloned into the pGEMT-EASY vector (Promega).

Polymerase Chain Reaction:

Article Title: New Real-Time Quantitative PCR Procedure for Quantification of Bifidobacteria in Human Fecal Samples
Article Snippet: .. The PCR mixture was composed of 1× PCR buffer II (Applied Biosystems, Foster City, Calif.) with 2.5 mM MgCl2 , 0.2 μM each primer, 200 μM each of the four deoxynucleoside triphosphates (Amersham Biosciences, Helsinki, Finland), and 1.25 U of AmpliTaq Gold DNA polymerase (Applied Biosystems). .. The thermal cycle program consisted of an initial cycle of 95°C for 10 min for denaturation and polymerase activation, 30 cycles of 95°C for 15 s, 60°C for 1 min, and 72°C for 45 s, and a final extension step of 10 min at 72°C.

Article Title: Detection and Enumeration of Aromatic Oxygenase Genes by Multiplex and Real-Time PCR
Article Snippet: .. All PCR mixtures contained 1× PCR buffer (Promega, Madison, Wis.), 0.2 mM each of the four deoxynucleoside triphosphates (Amersham Pharmacia, Piscataway, N.J.), and 1 U of Taq polymerase. ..

Article Title: CDC25A Phosphatase Is a Target of E2F and Is Required for Efficient E2F-Induced S Phase
Article Snippet: .. In addition, a PCR sample contained deoxynucleoside triphosphates 50 μM each, buffer with MgCl2 (1.5 mM final concentration), primers (0.2 μM each), [32 P]dCTP (0.1 μl of 3,000 Ci/mM; Amersham Life Science), Taq polymerase (1 U; Perkin-Elmer), and water (to a final volume of 30 μl). .. To perform semiquantitative PCR, for each couple of primers we determined on cDNA from cycling Rat1 RNA (at the best annealing temperature) how many cycles were required to detect a clear signal in the linear range.

Article Title: Desulfitobacterium hafniense Is Present in a High Proportion within the Biofilms of a High-Performance Pentachlorophenol-Degrading, Methanogenic Fixed-Film Reactor
Article Snippet: .. PCR mixtures of 50 μl contained 10 mM Tris-HCl buffer (pH 9.0), 1.5 mM MgCl2 , 50 mM KCl, 200 μM deoxynucleoside triphosphates, 2.5 U of Taq polymerase (Amersham Biosciences, Baie d'Urfé, Québec, Canada), 10 pmol of each primer (Table ), 0.5 μg of bovine serum albumin per μl, and approximately 10 ng of DNA. .. Amplifications were carried out with a model 480 Thermal Cycler (Perkin-Elmer) starting with a 5-min step at 80°C during which DNA was added, and then the reaction went to 94°C for 5 min and 55°C for 5 min, followed by 30 to 35 cycles at 72°C for 2 min, 94°C for 40 s, and 55°C for 1 min and a final extension at 72°C for 10 min.

Chloramphenicol Acetyltransferase Assay:

Article Title: Comparative Evaluation of Three Different Genotyping Methods for Investigation of Nosocomial Outbreaks of Legionnaires' Disease in Hospitals
Article Snippet: .. The amplification reaction was performed in a final volume of 25 μl containing 1 μl of the diluted DNA, 75 ng of the Cy-5 fluorescently labeled Pst I-G primer (5′-GAC TGC GTA CAT GCA GG), 0.2 mM deoxynucleoside triphosphates (both from Amersham Pharmacia Biotech), 5 mM MgCl2 , 10 mM Tris-HCl (pH 9.0), 50 mM KCl, and 1 U of Taq DNA polymerase (Boehringer Mannheim). .. The PCR comprised 33 cycles of 60 s at 94°C, 60 s at 60°C, and 150 s at 72°C and a final extension step at 72°C for 5 min.

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    GE Healthcare deoxynucleoside triphosphates dntps
    Deoxynucleoside Triphosphates Dntps, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphates dntps/product/GE Healthcare
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphates dntps - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    GE Healthcare deoxynucleoside triphosphates
    Deoxynucleoside Triphosphates, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphates/product/GE Healthcare
    Average 92 stars, based on 108 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphates - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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