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Eppendorf AG deoxynucleoside triphosphates
Deoxynucleoside Triphosphates, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 92/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxynucleoside triphosphates/product/Eppendorf AG
Average 92 stars, based on 26 article reviews
Price from $9.99 to $1999.99
deoxynucleoside triphosphates - by Bioz Stars, 2020-07
92/100 stars

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Polymerase Chain Reaction:

Article Title: pH Gradient-Induced Heterogeneity of Fe(III)-Reducing Microorganisms in Coal Mining-Associated Lake Sediments ▿pH Gradient-Induced Heterogeneity of Fe(III)-Reducing Microorganisms in Coal Mining-Associated Lake Sediments ▿ †
Article Snippet: .. DNA extracts from zone I and III sediment samples were PCR amplified with primers GM3 and GM4 in a reaction mixture consisting of 20 pmol of each primer, 200 μM deoxynucleoside triphosphates, 300 μg of bovine serum albumin, 1× PCR buffer, and 1 U of Taq DNA polymerase (Eppendorf, Hamburg, Germany). .. Template DNA (4 μl) was preheated to 70°C prior to addition to the PCR mixture.

Article Title: Development and Validation of a Diagnostic DNA Microarray To Detect Quinolone-Resistant Escherichia coli among Clinical Isolates
Article Snippet: .. The 50-μl PCR mixture included approximately 80 ng of template (genomic DNA of E. coli ), a 0.4 pM concentration of each primer, 0.1 mM deoxynucleoside triphosphates (the ratio between dCTP and Cy5-dCTP was 3:2), 1.5 mM Mg2+ , and 2.5 U of Taq polymerase (Eppendorf). .. The same parameters as described above were used for the labeling PCRs.

Article Title: Characterization of primary neurospheres generated from mouse ventral rostral hindbrain
Article Snippet: .. The polymerase chain reaction (PCR) was performed in 30-µl reactions containing 0.2 µM specific primers, 200 µM deoxynucleoside-triphosphates (Eppendorf), 10× reaction buffer, 2 mM MgCl2 , 1 U Taq polymerase (Fermentas), and 1 µl of the RT reaction as template. .. Amplification was carried out under the following conditions: initial denaturation for 10 min at 95°C, denaturation for 1 min at 94°C, primer annealing for 40 s (at the appropriate temperature for each primer pair), and elongation for 1 min at 72°C.

Article Title: Dedifferentiation derived cells exhibit phenotypic and functional characteristics of epidermal stem cells
Article Snippet: .. PCR The PCR reactions were performed in a total volume of 25 μl containing 2 μl DNA template, 10 pmol of each primer, 250 μM deoxynucleoside triphosphates, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 and 0.5 U Super Taq DNA Polymerase (Eppendorf, Hamburg, Germany). .. The amplification profiles were as follows: one step at 94°C for 2 min., then 40 cycles 94°C for 30 sec., 58°C for 40 sec. and 72°C for 1 min. and one final step at 72°C for 10 min.

Article Title: Development and Validation of a Diagnostic DNA Microarray To Detect Quinolone-Resistant Escherichia coli among Clinical Isolates
Article Snippet: .. The 50-μl PCR mixture included approximately 80 ng of template (genomic DNA of E. coli ), a 0.4 pM concentration of each primer, 0.25 mM deoxynucleoside triphosphates, 1.5 mM Mg2+ , and 2.5 U of Taq polymerase (Eppendorf, Hamburg, Germany). .. The PCRs were performed in a thermocycler (Mastercycler gradient) (Eppendorf) with the following parameters: 94°C for 5 min; 30 cycles at 94°C for 1 min, 52°C for 1 min, and 72°C for 1 min; and a final elongation at 72°C for 10 min.

Article Title: CCR4-Associated Factor CAF1 Is an Essential Factor for Spermatogenesis
Article Snippet: .. For PCR, 2 μl of the reverse-transcribed cDNA template (2 μg) was added to a final volume of 20 μl of reaction buffer containing 1.5 mM MgCl2 , 50 mM KCl, 0.2 mM deoxynucleoside triphosphates, 15 pM each primer, and 0.5 U of Taq polymerase in 3 μl of Master Taq polymerase enhancer (Eppendorf). ..

Concentration Assay:

Article Title: Development and Validation of a Diagnostic DNA Microarray To Detect Quinolone-Resistant Escherichia coli among Clinical Isolates
Article Snippet: .. The 50-μl PCR mixture included approximately 80 ng of template (genomic DNA of E. coli ), a 0.4 pM concentration of each primer, 0.1 mM deoxynucleoside triphosphates (the ratio between dCTP and Cy5-dCTP was 3:2), 1.5 mM Mg2+ , and 2.5 U of Taq polymerase (Eppendorf). .. The same parameters as described above were used for the labeling PCRs.

Article Title: Development and Validation of a Diagnostic DNA Microarray To Detect Quinolone-Resistant Escherichia coli among Clinical Isolates
Article Snippet: .. The 50-μl PCR mixture included approximately 80 ng of template (genomic DNA of E. coli ), a 0.4 pM concentration of each primer, 0.25 mM deoxynucleoside triphosphates, 1.5 mM Mg2+ , and 2.5 U of Taq polymerase (Eppendorf, Hamburg, Germany). .. The PCRs were performed in a thermocycler (Mastercycler gradient) (Eppendorf) with the following parameters: 94°C for 5 min; 30 cycles at 94°C for 1 min, 52°C for 1 min, and 72°C for 1 min; and a final elongation at 72°C for 10 min.

Amplification:

Article Title: pH Gradient-Induced Heterogeneity of Fe(III)-Reducing Microorganisms in Coal Mining-Associated Lake Sediments ▿pH Gradient-Induced Heterogeneity of Fe(III)-Reducing Microorganisms in Coal Mining-Associated Lake Sediments ▿ †
Article Snippet: .. DNA extracts from zone I and III sediment samples were PCR amplified with primers GM3 and GM4 in a reaction mixture consisting of 20 pmol of each primer, 200 μM deoxynucleoside triphosphates, 300 μg of bovine serum albumin, 1× PCR buffer, and 1 U of Taq DNA polymerase (Eppendorf, Hamburg, Germany). .. Template DNA (4 μl) was preheated to 70°C prior to addition to the PCR mixture.

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    Eppendorf AG deoxynucleoside triphosphates
    Deoxynucleoside Triphosphates, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 92/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphates/product/Eppendorf AG
    Average 92 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphates - by Bioz Stars, 2020-07
    92/100 stars
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