deoxynucleoside triphosphates (Boehringer Mannheim)

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Boehringer Mannheim deoxynucleoside triphosphates
Deoxynucleoside Triphosphates, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxynucleoside triphosphates/product/Boehringer Mannheim
Average 92 stars, based on 79 article reviews
Price from $9.99 to $1999.99

deoxynucleoside triphosphates - by Bioz Stars, 2021-01

92/100 stars

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Polymerase Chain Reaction:

Article Title: Analysis of vacA, cagA, and IS605 Genotypes and Those Determined by PCR Amplification of DNA between Repetitive Sequences of Helicobacter pylori Strains Isolated from Patients with Nonulcer Dyspepsia or Mucosa-Associated Lymphoid Tissue Lymphoma
Article Snippet: .. Each 25-μl PCR mixture contained 5 pmol of primers IS-5 and IS-1 (Perkin-Elmer Applied Biosystems UK, Warrington, United Kingdom), 0.625 U of AmpliTaq DNA polymerase (Perkin-Elmer Applied Biosystems UK), 200 μM deoxynucleoside triphosphates (Boehringer Mannheim UK Ltd., Lewes, United Kingdom), PCR buffer II with 2.5 mM MgCl2 (Perkin-Elmer Applied Biosystems UK), and approximately 200 ng of genomic DNA. .. A touchdown PCR was performed in a thermal cycler (Cyclogene, Techne Ltd., Cambridge, United Kingdom).

Article Title: Mapping of IS6110 Insertion Sites in Two Epidemic Strains of Mycobacterium tuberculosis
Article Snippet: .. RT mixtures consisted of total RNA, 2 μl of 10× PCR buffer (500 mM KCl, 100 mM Tris [pH 8.3], 0.1% gelatin), 10 pmol of random hexamer oligonucleotides (Amersham Pharmacia Biotech, Piscataway, N.J.), 1 μl of 40 mM deoxynucleoside triphosphates (10 mM [each] dATP, dCTP, dGTP, and dTTP), 0.5 μg of RNase-free bovine serum albumin, 1 μl of Prime RNase inhibitor, and 2.5 U of avian myeloblastosis virus reverse transcriptase (Boehringer-Mannheim, Roche Molecular Biochemicals, Indianapolis, Ind.) in a 20-μl total volume. .. RT was performed at 42°C for 1 h followed by 95°C for 5 min. RT-PCR controls included the above reaction without avian myeloblastosis virus reverse transcriptase to monitor for the presence of contaminating DNA.

Article Title: Characterization and Complete Genome Sequence of a Novel Coronavirus, Coronavirus HKU1, from Patients with Pneumonia
Article Snippet: .. The PCR mixture (50 μl) contained cDNA, PCR buffer (10 mM Tris-HCl [pH 8.3], 50 mM KCl, 3 mM MgCl2 , 0.01% gelatin), 200 μM (each) deoxynucleoside triphosphates, and 1.0 U of Taq polymerase (Boehringer, Mannheim, Germany). .. The mixtures were amplified in 40 cycles of 94°C for 1 min, 48°C for 1 min, and 72°C for 1 min and a final extension at 72°C for 10 min in an automated thermal cycler (Perkin-Elmer Cetus, Gouda, The Netherlands).

Article Title: Incidence and Characterization of Integrons, Genetic Elements Mediating Multiple-Drug Resistance, in Avian Escherichia coli
Article Snippet: .. This reaction mixture consisted of 0.2 mM deoxynucleoside triphosphates, 2.0 mM MgCl2 , 1× PCR buffer (50 mM Tris [pH 7.4]), bovine serum albumin (0.25 mg/ml), 50 pmol (each) of forward and reverse PCR primers, and 0.5 units of Taq DNA polymerase (Boehringer Mannheim, Indianapolis, Ind.). ..

Article Title: Molecular Characterization of Methanotrophic Isolates from Freshwater Lake Sediment
Article Snippet: .. The PCR reactions contained final concentrations of 1× PCR buffer (Gibco-BRL, Rockville, Md.), 1.5 mM MgCl2 (Gibco-BRL), 333 nM A189gc, 333 nM mb661, 0.167 mM deoxynucleoside triphosphates (Boehringer Mannheim), and 2.5 U of Taq polymerase (Gibco-BRL) in a total volume of 30 μl. .. For each LW strain, the pmoA fragments were then cloned into pCR2.1 using the Topo-TA Cloning Kit (Invitrogen, San Diego, Calif.), and 8 to 10 separate transformants with inserts were analyzed for each pmoA. pmoA fragments were reamplified as noted above, except the initial denaturation step was extended to 5 min, and these PCR products were digested with Hha I (Gibco-BRL) and a combination of Msp I (Gibco-BRL) and Hae III (Gibco-BRL) in a total of 5 μl for each digestion.

Random Hexamer Labeling:

Amplification:

Article Title: Comparison of C18-Carboxypropylbetaine and Glass Bead DNA Extraction Methods for Detection of Mycobacterium bovis in Bovine Milk Samples and Analysis of Samples by PCR
Article Snippet: .. Each amplification contained 3 μl of sample in 1× Amplificasa reaction buffer (Biotecnologias Universitarias, México, D.F., México) supplemented with 1.25 mM MgCl2 (Life Technologies, Inc.), 50 μM deoxynucleoside triphosphates (Boehringer, Mannheim, Germany), 0.2 μM (each) IS 6110 primer as described by Eisenach et al. , and 1.25 U of Amplificasa Taq polymerase (Biotecnologias Universitarias). .. The amplification protocol entailed denaturation, annealing, and extension steps at 96, 65, and 72°C, respectively, for 1 min each.