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Toyobo deoxynucleoside triphosphates dntps
Deoxynucleoside Triphosphates Dntps, supplied by Toyobo, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxynucleoside triphosphates dntps/product/Toyobo
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
deoxynucleoside triphosphates dntps - by Bioz Stars, 2020-08
92/100 stars

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Polymerase Chain Reaction:

Article Title: Application of a Euryarchaeota-Specific Helicase from Thermococcus kodakarensis for Noise Reduction in PCR
Article Snippet: .. PCR was performed in a mixture (50 μl) containing 120 mM Tris HCl (pH 8.0), 10 mM KCl, 6 mM (NH4 )2 SO4 , 0.1% Triton X-100, 10 μg · ml−1 bovine serum albumin (BSA), 0.2 mM deoxynucleoside triphosphates (dNTPs), 1 mM MgSO4 , 0.2 μM (each) primers, 50 ng of template DNA, and 1 U of KOD-Plus DNA polymerase (Toyobo, Osaka, Japan) in a thermal cycler as follows: 2 min at 98°C, followed by 17 cycles of 15 s at 98°C, 30 s at 55°C, and 8.5 min at 68°C. .. These amplified fragments of the Tk0566 and Tk0928 genes were separately cloned into the NdeI/EcoRI sites of pET28a and in the NdeI/SalI sites of pET28a, yielding the plasmids pHisTK0566 and pHisTK0928, respectively.

Article Title: The broad bacterial blight resistance of rice line CBB23 is triggered by a novel transcription activator‐like ( TAL) effector of Xanthomonas oryzae pv. oryzae
Article Snippet: .. The reaction (20 μL) contained 50 ng of template DNA, 1 × PCR buffer, 0.6 mmol/L deoxynucleoside triphosphates (dNTPs), 0.15 mmol/L of each primer and 0.75 U KOD Taq polymerase (TOYOBO, Osaka, Japan). .. PCR was initiated at 95 °C for 3 min, followed by 35 cycles of amplification at 94 °C for 40 s, 60 °C for 40 s, 72 °C for 1 min, and a final extension at 72 °C for 10 min. For Southern blot analysis, 1 μg of bacterial genomic DNA was digested with Sph I alone or together with Sau3 AI, separated on a 1.2% (w/v) agarose gel and transferred to a nylon membrane (Hybond‐N+, Amersham Pharmacia Bio‐tech, Amersham, UK).

Labeling:

Article Title: Pmr, a Histone-Like Protein H1 (H-NS) Family Protein Encoded by the IncP-7 Plasmid pCAR1, Is a Key Global Regulator That Alters Host Function ▿Pmr, a Histone-Like Protein H1 (H-NS) Family Protein Encoded by the IncP-7 Plasmid pCAR1, Is a Key Global Regulator That Alters Host Function ▿ ¶
Article Snippet: .. The extension reaction was performed with 4 μl of 5× First Strand buffer containing 10 μg of total RNA, 2 pmol of the labeled primer, 100 U of SuperScript III reverse transcriptase (Invitrogen, Carlsbad, CA), 40 U of RNaseOUT (Invitrogen), 10 mM dithiothreitol (DTT), and 0.5 mM deoxynucleoside triphosphates (dNTPs) (Toyobo). .. After denaturation of the RNA and the labeled primer at 65°C for 5 min, the remaining reagents were added, and then the mixture was incubated at 50°C for 30 min.

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    Toyobo deoxynucleoside triphosphates dntps
    Deoxynucleoside Triphosphates Dntps, supplied by Toyobo, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphates dntps/product/Toyobo
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphates dntps - by Bioz Stars, 2020-08
    92/100 stars
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