deoxynucleoside triphosphates dntps  (Thermo Fisher)


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    Structured Review

    Thermo Fisher deoxynucleoside triphosphates dntps
    Deoxynucleoside Triphosphates Dntps, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphates dntps/product/Thermo Fisher
    Average 93 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphates dntps - by Bioz Stars, 2020-07
    93/100 stars

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    Related Articles

    SYBR Green Assay:

    Article Title: Carbapenem Resistance in Elizabethkingia meningoseptica Is Mediated by Metallo-?-Lactamase BlaB
    Article Snippet: .. Total RNA was purified from cells with TRIzol reagent (Sigma) according to the manufacturer's instructions, treated with RNase-free DNase (Promega), and then converted to cDNA using a mixture containing SuperScript III reverse transcriptase (Invitrogen), random hexanucleotides, and RNaseOUT inhibitor (Invitrogen). cDNA samples were appropriately diluted and subjected to qRT-PCR with a reaction mixture containing 1× reaction buffer, 0.4× SYBR green, 3 mM MgCl2 , 0.2 mM deoxynucleoside triphosphates (dNTPs), 1 U Platinum Taq (Invitrogen), and 1 μM primers ( , primers 3 to 10). .. The cycling parameters were as follows: 1 cycle of 95°C for 1 min; 40 cycles of 95°C for 15 s, 55°C for 30 s, and 72°C for 40 s; and 1 cycle (melting curve) of 95°C for 15 s, 60°C for 15 s, a gradient for 20 min, and 95°C for 15 s. Changes in gene expression were determined by the comparative threshold cycle ( C T ) method ( ).

    Amplification:

    Article Title: Novel Bacterial Isolate from Permian Groundwater, Capable of Aggregating Potential Biofuel-Producing Microalga Nannochloropsis oceanica IMET1
    Article Snippet: .. The HW001-specific gene was amplified by PCR using a 50-μl reaction mixture that included 37.8 μl of sterilized distilled water, 5 μl of 10× high-fidelity PCR buffer, 2 μl of MgSO4 (50 mM), 1 μl of a mix of deoxynucleoside triphosphates (dNTPs) (2.5 mM each), 1 μl of HW001F and HW001R primer (10 μM each), 0.2 μl of Platinum Taq DNA (Invitrogen Life Technologies, Carlsbad, CA), and 2 μl of template DNA. .. The PCR amplification was conducted as follows.

    Article Title: Hepatitis C Virus Envelope Glycoprotein Fitness Defines Virus Population Composition following Transmission to a New Host
    Article Snippet: .. Amplification reactions were set up in 20-μl volumes containing 4 pmol of primer OAS 1a and primer EOS , 200 mM deoxynucleoside triphosphates (dNTPs), 0.5 U of Platinum Taq High Fidelity polymerase (Invitrogen), 1× High Fidelity polymerase buffer, and 2 mM MgSO4 . .. The PCR cycling parameters were as follows: initial denaturation at 94°C for 2 min, followed by 35 cycles of 94°C for 15 s, 50°C for 30 s, and 68°C for 3 min, with a final extension step at 68°C for 10 min. Two microliters of first-round product was subsequently used as the template in second-round reactions with primers 1ASGT1a and 170gt1, using amplification and cycling conditions identical to the first round but increasing the cycle number to 45.

    Generated:

    Article Title: Lund Human Mesencephalic (LUHMES) Neuronal Cell Line Supports Herpes Simplex Virus 1 Latency In Vitro
    Article Snippet: .. To this mixture, 1 μg RNA was added and heated for 3 min at 80°C, and then primer annealing was performed for 45 min at 50°C. cDNA was generated by adding MMLV RT, deoxynucleoside triphosphates (dNTPs), and RT buffer in a final reaction volume of 25 μl and incubated at 50°C for 45 min. RT enzyme was inactivated by incubation at 92°C for 10 min and qPCR performed using the following Applied Biosystems TaqMan assays: human GAPDH (catalog no. 4331182) and Assay ID s02786624_g1. ..

    Quantitative RT-PCR:

    Article Title: Carbapenem Resistance in Elizabethkingia meningoseptica Is Mediated by Metallo-?-Lactamase BlaB
    Article Snippet: .. Total RNA was purified from cells with TRIzol reagent (Sigma) according to the manufacturer's instructions, treated with RNase-free DNase (Promega), and then converted to cDNA using a mixture containing SuperScript III reverse transcriptase (Invitrogen), random hexanucleotides, and RNaseOUT inhibitor (Invitrogen). cDNA samples were appropriately diluted and subjected to qRT-PCR with a reaction mixture containing 1× reaction buffer, 0.4× SYBR green, 3 mM MgCl2 , 0.2 mM deoxynucleoside triphosphates (dNTPs), 1 U Platinum Taq (Invitrogen), and 1 μM primers ( , primers 3 to 10). .. The cycling parameters were as follows: 1 cycle of 95°C for 1 min; 40 cycles of 95°C for 15 s, 55°C for 30 s, and 72°C for 40 s; and 1 cycle (melting curve) of 95°C for 15 s, 60°C for 15 s, a gradient for 20 min, and 95°C for 15 s. Changes in gene expression were determined by the comparative threshold cycle ( C T ) method ( ).

    Purification:

    Article Title: Carbapenem Resistance in Elizabethkingia meningoseptica Is Mediated by Metallo-?-Lactamase BlaB
    Article Snippet: .. Total RNA was purified from cells with TRIzol reagent (Sigma) according to the manufacturer's instructions, treated with RNase-free DNase (Promega), and then converted to cDNA using a mixture containing SuperScript III reverse transcriptase (Invitrogen), random hexanucleotides, and RNaseOUT inhibitor (Invitrogen). cDNA samples were appropriately diluted and subjected to qRT-PCR with a reaction mixture containing 1× reaction buffer, 0.4× SYBR green, 3 mM MgCl2 , 0.2 mM deoxynucleoside triphosphates (dNTPs), 1 U Platinum Taq (Invitrogen), and 1 μM primers ( , primers 3 to 10). .. The cycling parameters were as follows: 1 cycle of 95°C for 1 min; 40 cycles of 95°C for 15 s, 55°C for 30 s, and 72°C for 40 s; and 1 cycle (melting curve) of 95°C for 15 s, 60°C for 15 s, a gradient for 20 min, and 95°C for 15 s. Changes in gene expression were determined by the comparative threshold cycle ( C T ) method ( ).

    Real-time Polymerase Chain Reaction:

    Article Title: Lund Human Mesencephalic (LUHMES) Neuronal Cell Line Supports Herpes Simplex Virus 1 Latency In Vitro
    Article Snippet: .. To this mixture, 1 μg RNA was added and heated for 3 min at 80°C, and then primer annealing was performed for 45 min at 50°C. cDNA was generated by adding MMLV RT, deoxynucleoside triphosphates (dNTPs), and RT buffer in a final reaction volume of 25 μl and incubated at 50°C for 45 min. RT enzyme was inactivated by incubation at 92°C for 10 min and qPCR performed using the following Applied Biosystems TaqMan assays: human GAPDH (catalog no. 4331182) and Assay ID s02786624_g1. ..

    Concentration Assay:

    Article Title: Comparative Phenotypic, Molecular, and Virulence Characterization of Vibrio parahaemolyticus O3:K6 Isolates
    Article Snippet: .. Each reaction mixture had a final concentration of 1× PCR buffer (Gibco), 1.5 mM MgCl2 , 50 μM deoxynucleoside triphosphates (dNTPs), 1 μM primer set, 1 U of Taq polymerase (PE Applied Biosystems, Foster City, Calif.), and 1 μl of template DNA. .. The following reaction conditions produced a single band of the expected size: preincubation at 94°C for 5 min; amplification for 40 cycles of 94°C for 45 s, 45°C for 45 s, and 72°C for 90 s; and a final hold at 72°C for 7 min. PCR products were purified from primers and nucleotides with a QIAquick PCR purification kit (Qiagen, Inc., Valencia, Calif.).

    Article Title: Use of Acyclovir for Suppression of Human Immunodeficiency Virus Infection Is Not Associated with Genotypic Evidence of Herpes Simplex Virus Type 2 Resistance to Acyclovir: Analysis of Specimens from Three Phase III Trials ▿
    Article Snippet: .. PCR mixtures (50 μl) contained 5 μl High Fidelity Taq buffer, 1 μl of 10 mM deoxynucleoside triphosphates (dNTPs), 2 μl of 50 mM MgSO4 providing a final Mg2+ concentration of 2.2 mM, 0.2 μl High Fidelity Taq Platinum thermostable polymerase compound containing Pyrococcus GB-D polymerase, 20 picomoles of each primer (all from Invitrogen, Carlsbad, CA), 5 to 10 μl of template DNA, 3 μl dimethyl sulfoxide (DMSO), and water as needed. .. PCR products were checked for the predicted molecular weight by gel electrophoresis and gel purified (Qiaquick; Qiagen, Valencia, CA).

    Incubation:

    Article Title: Lund Human Mesencephalic (LUHMES) Neuronal Cell Line Supports Herpes Simplex Virus 1 Latency In Vitro
    Article Snippet: .. To this mixture, 1 μg RNA was added and heated for 3 min at 80°C, and then primer annealing was performed for 45 min at 50°C. cDNA was generated by adding MMLV RT, deoxynucleoside triphosphates (dNTPs), and RT buffer in a final reaction volume of 25 μl and incubated at 50°C for 45 min. RT enzyme was inactivated by incubation at 92°C for 10 min and qPCR performed using the following Applied Biosystems TaqMan assays: human GAPDH (catalog no. 4331182) and Assay ID s02786624_g1. ..

    Article Title: Mutation of a Broadly Conserved Operon (RL3499-RL3502) from Rhizobium leguminosarum Biovar viciae Causes Defects in Cell Morphology and Envelope Integrity ▿ Causes Defects in Cell Morphology and Envelope Integrity ▿ †
    Article Snippet: .. Samples were snap cooled on ice and 2 μl of 10 mM deoxynucleoside triphosphates (dNTPs) was added, followed by incubation at 37°C for 5 min. One microliter of RevertAid Moloney murine leukemia virus (M-MuLV) reverse transcriptase (Fermentas) was added, and samples were incubated at 42°C for 50 min, followed by heat inactivation at 70°C for 15 min. PCR mixtures contained 1× reaction buffer, 2 mM MgSO4, 0.2 mM dNTPs, 2 μM forward primer, 0.12 μM reverse primer, and 1 U Taq DNA polymerase (UBI, Calgary, Alberta, Canada). ..

    Polymerase Chain Reaction:

    Article Title: Comparative Phenotypic, Molecular, and Virulence Characterization of Vibrio parahaemolyticus O3:K6 Isolates
    Article Snippet: .. Each reaction mixture had a final concentration of 1× PCR buffer (Gibco), 1.5 mM MgCl2 , 50 μM deoxynucleoside triphosphates (dNTPs), 1 μM primer set, 1 U of Taq polymerase (PE Applied Biosystems, Foster City, Calif.), and 1 μl of template DNA. .. The following reaction conditions produced a single band of the expected size: preincubation at 94°C for 5 min; amplification for 40 cycles of 94°C for 45 s, 45°C for 45 s, and 72°C for 90 s; and a final hold at 72°C for 7 min. PCR products were purified from primers and nucleotides with a QIAquick PCR purification kit (Qiagen, Inc., Valencia, Calif.).

    Article Title: Novel Bacterial Isolate from Permian Groundwater, Capable of Aggregating Potential Biofuel-Producing Microalga Nannochloropsis oceanica IMET1
    Article Snippet: .. The HW001-specific gene was amplified by PCR using a 50-μl reaction mixture that included 37.8 μl of sterilized distilled water, 5 μl of 10× high-fidelity PCR buffer, 2 μl of MgSO4 (50 mM), 1 μl of a mix of deoxynucleoside triphosphates (dNTPs) (2.5 mM each), 1 μl of HW001F and HW001R primer (10 μM each), 0.2 μl of Platinum Taq DNA (Invitrogen Life Technologies, Carlsbad, CA), and 2 μl of template DNA. .. The PCR amplification was conducted as follows.

    Article Title: Use of Acyclovir for Suppression of Human Immunodeficiency Virus Infection Is Not Associated with Genotypic Evidence of Herpes Simplex Virus Type 2 Resistance to Acyclovir: Analysis of Specimens from Three Phase III Trials ▿
    Article Snippet: .. PCR mixtures (50 μl) contained 5 μl High Fidelity Taq buffer, 1 μl of 10 mM deoxynucleoside triphosphates (dNTPs), 2 μl of 50 mM MgSO4 providing a final Mg2+ concentration of 2.2 mM, 0.2 μl High Fidelity Taq Platinum thermostable polymerase compound containing Pyrococcus GB-D polymerase, 20 picomoles of each primer (all from Invitrogen, Carlsbad, CA), 5 to 10 μl of template DNA, 3 μl dimethyl sulfoxide (DMSO), and water as needed. .. PCR products were checked for the predicted molecular weight by gel electrophoresis and gel purified (Qiaquick; Qiagen, Valencia, CA).

    Article Title: Multilocus Sequence Typing Subtypes of Poultry Clostridium perfringens Isolates Demonstrate Disease Niche Partitioning ▿
    Article Snippet: .. PCR mixtures contained 5 μl 10× PCR buffer, 1.5 μl 50 mM MgCl2 , 1 μl 10 mM deoxynucleoside triphosphates (dNTPs), 10 pmol each primer, 2.5 U Platinum Taq (Invitrogen, Carlsbad, CA), 1 μl template DNA (not quantified), and sterile distilled H2 O to a final volume of 50 μl. ..

    Article Title: Mutation of a Broadly Conserved Operon (RL3499-RL3502) from Rhizobium leguminosarum Biovar viciae Causes Defects in Cell Morphology and Envelope Integrity ▿ Causes Defects in Cell Morphology and Envelope Integrity ▿ †
    Article Snippet: .. Samples were snap cooled on ice and 2 μl of 10 mM deoxynucleoside triphosphates (dNTPs) was added, followed by incubation at 37°C for 5 min. One microliter of RevertAid Moloney murine leukemia virus (M-MuLV) reverse transcriptase (Fermentas) was added, and samples were incubated at 42°C for 50 min, followed by heat inactivation at 70°C for 15 min. PCR mixtures contained 1× reaction buffer, 2 mM MgSO4, 0.2 mM dNTPs, 2 μM forward primer, 0.12 μM reverse primer, and 1 U Taq DNA polymerase (UBI, Calgary, Alberta, Canada). ..

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  • 94
    Thermo Fisher deoxynucleoside triphosphates
    Deoxynucleoside Triphosphates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 708 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphates/product/Thermo Fisher
    Average 94 stars, based on 708 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphates - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    93
    Thermo Fisher deoxynucleoside triphosphates dntps
    Deoxynucleoside Triphosphates Dntps, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphates dntps/product/Thermo Fisher
    Average 93 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphates dntps - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

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