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Promega deoxynucleoside triphosphates dntps
Deoxynucleoside Triphosphates Dntps, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxynucleoside triphosphates dntps/product/Promega
Average 92 stars, based on 80 article reviews
Price from $9.99 to $1999.99
deoxynucleoside triphosphates dntps - by Bioz Stars, 2020-07
92/100 stars

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Amplification:

Article Title: Association of Nucleotide Polymorphisms within the O-Antigen Gene Cluster of Escherichia coli O26, O45, O103, O111, O121, and O145 with Serogroups and Genetic Subtypes
Article Snippet: .. Amplification reaction mixtures contained 3.2 μl deoxynucleoside triphosphates (dNTPs) (1.25 mM; Promega, Madison, WI), 2 μl 10× buffer (HotStarTaq DNA polymerase kit; Qiagen), 0.1 μl HotStarTaq DNA polymerase (5 U/μl; Qiagen), 0.2 μl each primer (30 μM), and 1 μl template DNA (5 ng/μl) in a 20-μl reaction volume. .. Amplifications were performed with an S1000 or Peltier Dyad Bio-Rad thermocycler (Hercules, CA) under the following conditions: an initial denaturation step at 95°C for 15 min, followed by 30 s at 94°C, 30 s at 52°C, and 2 min at 72°C for 35 cycles and a final extension step at 72°C for 10 min. Amplification was verified by running PCR products on a 1% agarose gel containing ethidium bromide.

Synthesized:

Article Title: Borrelia burgdorferi RNA Induces Type I and III Interferons via Toll-Like Receptor 7 and Contributes to Production of NF-κB-Dependent Cytokines
Article Snippet: .. RNA samples were stored at −80°C after the addition of 0.8 μl of RNase inhibitor (40 U/μl; Promega). cDNA was synthesized from 1.0 μg of total RNA in 20-μl reaction mixtures containing deoxynucleoside triphosphates (dNTPs) (250 μM each), 0.5 μg random primers, 5 U avian myeloblastosis virus (AMV) reverse transcriptase (RT), 10 U of RNase inhibitor, and 1× RT buffer (all from Promega). ..

Reverse Transcription Polymerase Chain Reaction:

Article Title: Molecular and antigenic characterization of group C orthobunyaviruses isolated in Peru
Article Snippet: .. RT-PCR was performed in a total volume of 50 μl containing 1 μM of each primer (forward and reverse; ), 200 μM of deoxynucleoside triphosphates (dNTPs) (Promega, Madison, WI), 5 μM dithiothreitol (DTT), 5 units of avian myeloblastosis virus reverse transcriptase (AMV RT; Promega, Madison, WI), 2.5 units of GoTaq DNA polymerase (Promega, Madison, WI), 1X green GoTaq reaction buffer (Promega, Madison, WI), and 10 μl of extracted RNA. .. For the partial S segment, reverse transcription was performed for 60 minutes at 42°C and 95°C for 2 minutes, followed by 35 PCR cycles, consisting of 94°C for 40 seconds, 50°C for 40 seconds, and 72°C for 50 seconds, and finally at 72°C for 5 minutes.

Concentration Assay:

Article Title: Amplification ratio control system for copy number variation genotyping
Article Snippet: .. Promega, Madison, WI, USA), 2.5 mM MgCl2 , UP at 1.0 μM, deoxynucleoside triphosphates (dNTPs) at 200 μM each, 0.25 U of ‘Go-Taq Flexi’ polymerase (Promega) per 5 μl reaction, EvaGreen fluorescent dye (Insight Biotechnology) at a final concentration of 0.2× per 5 μl reaction, and 0.7 μl water (to 5 μl total volume) per microplate well. .. Thermal cycling parameters were as follows: 94°C 2 min; three cycles (for the sequence-specific primers) of: 94°C 1 min, 49°C 1 min, 72°C 1 min; 28 cycles (for UP) of: 94°C 30 s, 67°C 30 s, 72°C 30 s; 72°C 2 min.

Incubation:

Article Title: Functional Genotyping of Sulfurospirillum spp. in Mixed Cultures Allowed the Identification of a New Tetrachloroethene Reductive Dehalogenase
Article Snippet: .. RNA (0.2 μg) was mixed with 0.5 μg of random hexamers (Microsynth GmbH, Balgach, Switzerland) in a total volume of 10 μl, incubated for 5 min at 70°C, and cooled on ice for 3 min. From this mixture, 5 μl was mixed with 4 μl of 5× RT buffer, 1 μl of 10 mM (each) deoxynucleoside triphosphates (dNTPs), 2.4 μl of 25 mM MgCl2 , 6.1 μl of RNase-free water, 0.5 μl of RNasin (Promega), and 1 μl of ImProm-II reverse transcriptase (Promega). ..

other:

Article Title: Biosynthesis of Cylindrospermopsin and 7-Epicylindrospermopsin in Oscillatoria sp. Strain PCC 6506: Identification of the cyr Gene Cluster and Toxin Analysis ▿
Article Snippet: Taq polymerase, buffer, and deoxynucleoside triphosphates (dNTPs) were obtained from Promega (Paris, France), and LongAmp polymerase was obtained from New England Biolabs (Ozyme, France).

Polymerase Chain Reaction:

Article Title: Specific Inhibition of Human Immunodeficiency Virus Type 1 (HIV-1) Integration in Cell Culture: Putative Inhibitors of HIV-1 Integrase
Article Snippet: .. PCRs (25 μl) were performed using ≈50 cell-equivalents of chromosomal DNA in 1× PCR Buffer II (Perkin-Elmer), 2 mM MgCl2 , 0.2 mM concentrations of deoxynucleoside triphosphates (dNTPs) (Promega), 25 pmol of β-glo 1 and 25 pmol of β-glo 2 primers (Table ) using 2.5 U of Amplitaq DNA polymerase. .. Reactions were cycled as follows: 94°C for 3 min; 25 cycles of 94°C for 45 s, 58°C for 30 s, 72°C for 45 s; and a final extension of 72°C for 10 min. Mitochondrial DNA was amplified and used to standardize the cell-equivalent amounts of DNA extracted in each HIRT supernatant fraction.

Article Title: Molecular Characterization of Diazotrophic and Denitrifying Bacteria Associated with Mangrove Roots ▿
Article Snippet: .. The PCR mixtures (20 μl) contained 2 μl of 10× buffer, 0.4 μl of 10 mM deoxynucleoside triphosphates (dNTPs), 1.2 μl of MgCl2 (25 mM), 20 pmol of each primer, 0.2 μl of Taq polymerase (Promega), and 100 ng of DNA. .. Colony PCR ( ) was used to amplify genes from the bacterial strains isolated from mangrove roots, increasing the initial temperature time of the PCR to 5 min. Each PCR product was visualized after electrophoresis in 1.5% Tris-borate-EDTA (TBE) agarose gels.

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  • 85
    Promega nmol deoxynucleoside triphosphates dntps
    Nmol Deoxynucleoside Triphosphates Dntps, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nmol deoxynucleoside triphosphates dntps/product/Promega
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nmol deoxynucleoside triphosphates dntps - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    92
    Promega deoxynucleoside triphosphates dntps
    Deoxynucleoside Triphosphates Dntps, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphates dntps/product/Promega
    Average 92 stars, based on 80 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphates dntps - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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