Structured Review

TaKaRa deoxynucleoside triphosphate
Deoxynucleoside Triphosphate, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 315 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxynucleoside triphosphate/product/TaKaRa
Average 99 stars, based on 315 article reviews
Price from $9.99 to $1999.99
deoxynucleoside triphosphate - by Bioz Stars, 2020-09
99/100 stars

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Amplification:

Article Title: Essential Domains for Ribonucleoprotein Complex Formation Required for Retrotransposition of Telomere-Specific Non-Long Terminal Repeat Retrotransposon SART1 †
Article Snippet: .. After heat inactivation at 80°C for 20 min, 1 μl of the reaction mixture was amplified by PCR for 35 cycles of 98°C for 20 s, 60°C for 30 s, and 72°C for 30 s, with an initial denaturation at 96°C for 2 min and a final extension at 72°C for 5 min. PCR mixtures contained 200 μM concentrations of each dNTP, 2 mM MgCl2 , 50 mM KCl, 0.5 U of Ex- Taq (TaKaRa), and 0.5 μM concentrations of each primer (see Table S1 in the supplemental material). .. Purified SART1 complex was overlaid onto 3.3 ml of 10 to 40% glycerol gradient in the HG500 buffer.

DNA Synthesis:

Article Title: Chemical Incorporation of Chain-Terminating Nucleoside Analogs as 3′-Blocking DNA Damage and Their Removal by Human ERCC1-XPF Endonuclease
Article Snippet: .. Resumption of DNA Synthesis by DNA Polymerase after the Removal of CTNAs The hybridized oligonucleotides (400 fmol) were first treated with ERCC1-XPF (230 fmol) in 10 µL of 50 mM Tris-HCl buffer (pH 8.0), containing 2 mM MgCl2 , 0.5 mM DTT and 0.1 mg·mL−1 BSA, at 25 °C for 16 h. To the reaction mixture was added 5 µL of 30 mM Tris-HCl buffer (pH 7.9), containing 150 mM NaCl, 30 mM MgCl2 , 30 mM DTT, 300 µM dNTPs and the Klenow fragment of Escherichia coli DNA polymerase I, lacking the 3′–5′ exonuclease activity (KF− , 0.1 unit, Takara Bio). ..

Purification:

Article Title: Failure of PCR to Detect Treponema pallidum ssp. pertenue DNA in Blood in Latent Yaws
Article Snippet: .. Reactions were carried out in a 50 μL volume containing 200 μM of dNTPs, 1x PCR buffer, 1.25 U of Takara Ex Taq polymerase (Clontech Laboratories, Inc., Mountain View, CA), and 20 μL of DNA in an Applied Biosystems GeneAmp PCR system using the following conditions: initial hold at 94°C for 3 min followed by 45 cycles of 94°C for 1 min, 63°C for 1 min, and 68°C for 2 min, with a final extension cycle at 68°C for 10 min. A no-template control and purified genomic DNAs from T . pallidum ssp. pallidum strain Nichols and T . pallidum ssp. pertenue strain CDC2575 were included in each run. .. The inner real-time PCR which amplifies a 185-bp fragment of the outer PCR amplicon above was performed using primers and TaqMan probes as described previously [ ].

Polymerase Chain Reaction:

Article Title: Failure of PCR to Detect Treponema pallidum ssp. pertenue DNA in Blood in Latent Yaws
Article Snippet: .. Reactions were carried out in a 50 μL volume containing 200 μM of dNTPs, 1x PCR buffer, 1.25 U of Takara Ex Taq polymerase (Clontech Laboratories, Inc., Mountain View, CA), and 20 μL of DNA in an Applied Biosystems GeneAmp PCR system using the following conditions: initial hold at 94°C for 3 min followed by 45 cycles of 94°C for 1 min, 63°C for 1 min, and 68°C for 2 min, with a final extension cycle at 68°C for 10 min. A no-template control and purified genomic DNAs from T . pallidum ssp. pallidum strain Nichols and T . pallidum ssp. pertenue strain CDC2575 were included in each run. .. The inner real-time PCR which amplifies a 185-bp fragment of the outer PCR amplicon above was performed using primers and TaqMan probes as described previously [ ].

Article Title: Essential Domains for Ribonucleoprotein Complex Formation Required for Retrotransposition of Telomere-Specific Non-Long Terminal Repeat Retrotransposon SART1 †
Article Snippet: .. After heat inactivation at 80°C for 20 min, 1 μl of the reaction mixture was amplified by PCR for 35 cycles of 98°C for 20 s, 60°C for 30 s, and 72°C for 30 s, with an initial denaturation at 96°C for 2 min and a final extension at 72°C for 5 min. PCR mixtures contained 200 μM concentrations of each dNTP, 2 mM MgCl2 , 50 mM KCl, 0.5 U of Ex- Taq (TaKaRa), and 0.5 μM concentrations of each primer (see Table S1 in the supplemental material). .. Purified SART1 complex was overlaid onto 3.3 ml of 10 to 40% glycerol gradient in the HG500 buffer.

Article Title: Gnathostoma spinigerum Mitochondrial Genome Sequence: a Novel Gene Arrangement and its Phylogenetic Position within the Class Chromadorea
Article Snippet: .. PCR was conducted in 25 μl using 2 mM MgCl2 , 0.2 mM each of dNTPs, 2.5 μl 10 × Taq buffer, 2.5 μM of each primer and 0.5 μl LA Taq DNA polymerase (5 U/μl, Takara) in a thermocycler (Biometra). .. The cycling conditions were: 92 °C for 2 min (initial denaturation), then 92 °C for 10 s (denaturation), 56 °C (10 kb) or 54 °C (4 kb) for 30 s (annealing) and 60 °C for 10 min (extension) for 10 cycles, followed by 92 °C for 10 s, 56 °C (~10 kb) or 54 °C (~4 kb) for 30 s (annealing), and 60 °C for 10 min for 20 cycles, with a cycle elongation of 10 s for each cycle and a final extension at 60 °C for 10 min. PCR products were sequenced at Sangon Company (Shanghai, China) using a primer walking strategy .

Activity Assay:

Article Title: Chemical Incorporation of Chain-Terminating Nucleoside Analogs as 3′-Blocking DNA Damage and Their Removal by Human ERCC1-XPF Endonuclease
Article Snippet: .. Resumption of DNA Synthesis by DNA Polymerase after the Removal of CTNAs The hybridized oligonucleotides (400 fmol) were first treated with ERCC1-XPF (230 fmol) in 10 µL of 50 mM Tris-HCl buffer (pH 8.0), containing 2 mM MgCl2 , 0.5 mM DTT and 0.1 mg·mL−1 BSA, at 25 °C for 16 h. To the reaction mixture was added 5 µL of 30 mM Tris-HCl buffer (pH 7.9), containing 150 mM NaCl, 30 mM MgCl2 , 30 mM DTT, 300 µM dNTPs and the Klenow fragment of Escherichia coli DNA polymerase I, lacking the 3′–5′ exonuclease activity (KF− , 0.1 unit, Takara Bio). ..

Marker:

Article Title: Small Molecular Contaminant and Microorganism Can Be Simultaneously Detected Based on Nanobody-Phage: Using Carcinogen Aflatoxin and Its Main Fungal Aspergillus Section Flavi spp. in Stored Maize for Demonstration
Article Snippet: .. DNA polymerase (iTaq), Mg2+ , dNTPs, 6× loading buffer, and DNA marker were bought from Takara Bio (Beijing, China). .. The anti-aflatoxins monoclonal antibody 1C11 (mAb 1C11) and V2–5 phage displaying nanobody specific for 1C11 were produced by our team ( ; ).

Plasmid Preparation:

Article Title: A transcription and translation-coupled DNA replication system using rolling-circle replication
Article Snippet: .. Assay of the TTcDR reaction The optimized composition of the TTcDR system was as follows: template plasmid DNA (1 ng/μl), dNTPs (0.3 mM each, Takara), [32 -P] dCTP (3.3 μM, PerkinElmer), magnesium acetate (7.9 mM, Wako), potassium glutamate (70 mM, Wako), spermidine (0.375 mM, Nakarai), dithiothreitol (6 mM, Nakarai), ATP (0.375 mM, GE Healthcare), GTP (0.25 mM, GE Healthcare), CTP (0.125 mM, GE Healthcare), UTP (0.125 mM, GE Healthcare), creatine phosphate (25 mM, Nakarai), E. coli tRNA mixture (0.518 μg/μl, Roche), 10-formyl-5,6,7,8-tetrahydrofolic acid (10 ng/μl), Cys (0.3 mM, Wako), Tyr (0.3 mM, Wako), the other 18 amino acids except for Cys and Tyr (0.36 mM, Wako), 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid (100 mM, pH 7.6, Sigma), ribosomes (1 μM), IF1 (25 μM), IF2 (1 μM), IF3 (4.9 μM), EF-G (1.1 μM), EF-Tu (80 μM), EF-Ts (3.3 μM), RF1 (0.05 μM), RF2 (0.05 μM), RF3 (0.17 μM), RRF (3.9 μM), AlaRS (730 nM), ArgRS (30 nM), AsnRS (420 nM), AspRS (120 nM), CysRS (20 nM), GlnRS (60 nM), GluRS (230 nM), GlyRS (90 nM), HisRS (90 nM), IleRS (370 nM), LeuRS (40 nM), LysRS (120 nM), MetRS (110 nM), PheRS (130 nM), ProRS (170 nM), SerRS (80 nM), ThrRS (80 nM), TrpRS (30 nM), TyrRS (150 nM), ValRS (20 nM), MTF (590 nM), creatine kinase (0.25 μM), myokinase (1.4 μM), nucleoside-diphosphate kinase (20 nM), pyrophosphatase (40 nM), yeast inorganic pyrophosphatase (0.2 mU/μl, New England BioLabs (NEB)), ribonuclease inhibitor (0.1 U/μl; Promega), and T7 RNA polymerase (0.42 U/μl; Takara). .. Most of the proteins described above were purified according to a previously described method except for yeast inorganic pyrophosphatase, ribonuclease inhibitor, and T7 RNA polymerase.

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    TaKaRa deoxynucleotide triphosphate dntp
    Deoxynucleotide Triphosphate Dntp, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 754 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleotide triphosphate dntp/product/TaKaRa
    Average 99 stars, based on 754 article reviews
    Price from $9.99 to $1999.99
    deoxynucleotide triphosphate dntp - by Bioz Stars, 2020-09
    99/100 stars
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