Structured Review

Stratagene deoxynucleoside triphosphate
Deoxynucleoside Triphosphate, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxynucleoside triphosphate/product/Stratagene
Average 92 stars, based on 22 article reviews
Price from $9.99 to $1999.99
deoxynucleoside triphosphate - by Bioz Stars, 2020-07
92/100 stars

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Polymerase Chain Reaction:

Article Title: Genetically Controlled Self-Aggregation of Cell-Surface-Engineered Yeast Responding to Glucose Concentration
Article Snippet: .. The PCR mixture (100 μl) was as follows: 0.8 μg of genomic DNA, 100 pmol of each primer, 0.2 mM each deoxynucleoside triphosphate, the reaction buffer supplied by the vendor, and 5 U of Pfu DNA polymerase (Stratagene, La Jolla, Calif.). ..

Article Title: Mitochondrial DNA Mutation Detection by Electrospray Mass Spectrometry
Article Snippet: .. The PCR mixture consisted of 1× PCR buffer II (Applied Biosystems), 1.5 mmol/L Mg2+ (Applied Biosystems), 0.4 mol/L betaine (Sigma-Aldrich), 0.2 mmol/L of each deoxynucleoside triphosphate (Stratagene), 250 nmol/L of each primer, and 4 U AmpliTaq Gold (Applied Biosystems). .. Total cellular DNA from each sample (1–1.5 ng) was used as the template in the PCR.

Article Title: Expression of hurP, a Gene Encoding a Prospective Site 2 Protease, Is Essential for Heme-Dependent Induction of bhuR in Bordetella bronchiseptica ▿
Article Snippet: .. The components for the PCR using RB50 genomic DNA as a template were the following: 1× EasyA buffer, 800 μM deoxynucleoside triphosphate [dNTP] mix, 200 nM each primer, 10% dimethylsulfoxide [DMSO], and 2.5 U of EasyA polymerase (Stratagene, La Jolla, CA). ..

Concentration Assay:

Article Title: Amylomaltase of Pyrobaculum aerophilum IM2 Produces Thermoreversible Starch Gels
Article Snippet: .. The reaction mixture of 100 ml contained 50 ng P. aerophilum IM2 genomic DNA, 10 pmol of each primer, a 0.2 mM concentration of each deoxynucleoside triphosphate, and 2.5 U Pfu turbo DNA polymerase (Stratagene, La Jolla, CA) in the supplied buffer. .. The PCR product was purified using a PCR purification spin kit (Sigma-Aldrich), digested by NdeI and BamHI, and ligated in equivalently digested pET15b vector (T7 promoter, N-terminal His6 tag, Ampr ; Novagen, Madison, WI).

Amplification:

Article Title: Stability of the Human Fragile X (CGG)n Triplet Repeat Array in Saccharomyces cerevisiae Deficient in Aspects of DNA Metabolism
Article Snippet: .. Amplification conditions were as follows: an initial denaturation at 95°C for 5 min followed by 35 cycles of 95°C for 30 s, 55°C for 45 s, and 70°C for 5 min. Each 20-μl reaction mixture contained 20 mM Tris-HCl (pH 8.8), 10 mM KCl, 1.5 mM MgCl2 , 10 μM (NH4 )2 SO4 , 0.1% Triton, 100 μg of bovine serum albumin per ml, 0.5 μM each oligonucleotide, 200 μM each deoxynucleoside triphosphate, 5% dimethyl sulfoxide, and 1 U of wild-type Pfu polymerase (Stratagene). .. Transformation was carried out as described above, except that cells were incubated overnight in YPD medium at 4°C to allow expression of G418 resistance.

other:

Article Title: Identification of Bacteriocin-Like Inhibitors from Rumen Streptococcus spp. and Isolation and Characterization of Bovicin 255 †
Article Snippet: Reactions (100 μl) were set up in thin-walled tubes (Gordon Technologies, Mississauga, Ontario, Canada) containing 100 ng of template DNA, 1× Pfu buffer, 50 pmol of each primer, 0.1 mM concentrations of each deoxynucleoside triphosphate, and 2.5 U of Pfu DNA polymerase (Stratagene, La Jolla, Calif.).

Article Title: Role of CCAA Nucleotide Repeats in Regulation of Hemoglobin and Hemoglobin-Haptoglobin Binding Protein Genes of Haemophilus influenzae
Article Snippet: The reaction was performed in 50 μl containing 2 mM MgCl2 , 0.2 mM (each) deoxynucleoside triphosphate, 10 pM (each) primer, and 2 U of Pfu DNA polymerase (Stratagene, La Jolla, Calif.).

Article Title: Prevalent Bacterial Species and Novel Phylotypes in Advanced Noma Lesions
Article Snippet: One microliter of the DNA template was added to a reaction mixture (50-μl final volume) containing 20 pmol of each primer, 40 nmol of each deoxynucleoside triphosphate, and 1 U of Taq 2000 polymerase (Stratagene, La Jolla, Calif.) in buffer containing Taqstart Antibody (Sigma Chemical Co.).

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    Stratagene deoxynucleoside triphosphate dntp
    Deoxynucleoside Triphosphate Dntp, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphate dntp/product/Stratagene
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphate dntp - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    88
    Stratagene 2 deoxynucleoside 5 triphosphate
    2 Deoxynucleoside 5 Triphosphate, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2 deoxynucleoside 5 triphosphate/product/Stratagene
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    2 deoxynucleoside 5 triphosphate - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

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