Structured Review

Roche deoxynucleoside triphosphate
Deoxynucleoside Triphosphate, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 196 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxynucleoside triphosphate/product/Roche
Average 92 stars, based on 196 article reviews
Price from $9.99 to $1999.99
deoxynucleoside triphosphate - by Bioz Stars, 2020-07
92/100 stars

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Amplification:

Article Title: First Detection and Genotyping of Human-Associated Microsporidia in Pigeons from Urban Parks
Article Snippet: .. PCR amplification was done by using a 25-μl reaction mix containing 50 ng of DNA, 0.2 mM of each deoxynucleoside triphosphate, 0.2 μM each of the A and B primers, 1 unit of Taq DNA polymerase (Roche Molecular Biochemicals), and the PCR buffer, including a final concentration of 1.5 mM MgCl2 . ..

Synthesized:

Article Title: Acute and Persistent Infection of Human Neural Cell Lines by Human Coronavirus OC43
Article Snippet: .. For PCR, 1 of 10 or 1 of 5 of the synthesized cDNAs was incubated in the presence of 20 pmol (HuCV-OC43 S1 and N) or 50 pmol (GAPDH) of the sense and antisense primers, 1.5 mM (HuCV-OC43 S1 or GAPDH) or 2.5 mM (HuCV-OC43 N) MgCl2 (Roche Diagnostics), 1× PCR buffer (10 mM Tris-HCl [pH 8.3], 50 mM KCl; Roche Diagnostics) and 0.4 mM (each) deoxynucleoside triphosphate (Li salt; Roche Diagnostics) at 94°C for 5 min and at 60°C (HuCV-OC43 N), 50°C (GAPDH), or 37°C (HuCV-OC43 S1) for another 5 min. After the addition of Expand high-fidelity PCR system DNA polymerase ( Taq and Pwo DNA polymerases) (2.5 U; Roche Diagnostics), 30 cycles of 2 min at 72°C, 1 min at 94°C, and 2 min at 60°C (HuCV-OC43 N), 50°C (GAPDH), or 37°C (HuCV-OC43 S1) were performed, with a final elongation step of 10 min at 72°C. .. The DNA amplicons were separated by electrophoresis in a 1.5% (wt/vol) agarose gel with ethidium bromide (1 μg/ml).

Purification:

Article Title: Bacterial Community Dynamics during Production of Registered Designation of Origin Salers Cheese as Evaluated by 16S rRNA Gene Single-Strand Conformation Polymorphism Analysis
Article Snippet: .. Each reaction tube contained 10 mM Tris-HCl (pH 8.5), 25 mM KCl, 5 mM (NH4 )2 SO4 , 2 mM MgSO4 , 0.1 μM concentrations of each primer, 2 μl of purified DNA, 0.2 mM concentrations of each deoxynucleoside triphosphate, and 1 U of Pwo polymerase (Roche) and was then adjusted to a total volume of 50 μl. .. The reaction mixture was placed in a 9700 thermocycler (Perkin-Elmer).

Concentration Assay:

Article Title: First Detection and Genotyping of Human-Associated Microsporidia in Pigeons from Urban Parks
Article Snippet: .. PCR amplification was done by using a 25-μl reaction mix containing 50 ng of DNA, 0.2 mM of each deoxynucleoside triphosphate, 0.2 μM each of the A and B primers, 1 unit of Taq DNA polymerase (Roche Molecular Biochemicals), and the PCR buffer, including a final concentration of 1.5 mM MgCl2 . ..

Incubation:

Article Title: Acute and Persistent Infection of Human Neural Cell Lines by Human Coronavirus OC43
Article Snippet: .. For PCR, 1 of 10 or 1 of 5 of the synthesized cDNAs was incubated in the presence of 20 pmol (HuCV-OC43 S1 and N) or 50 pmol (GAPDH) of the sense and antisense primers, 1.5 mM (HuCV-OC43 S1 or GAPDH) or 2.5 mM (HuCV-OC43 N) MgCl2 (Roche Diagnostics), 1× PCR buffer (10 mM Tris-HCl [pH 8.3], 50 mM KCl; Roche Diagnostics) and 0.4 mM (each) deoxynucleoside triphosphate (Li salt; Roche Diagnostics) at 94°C for 5 min and at 60°C (HuCV-OC43 N), 50°C (GAPDH), or 37°C (HuCV-OC43 S1) for another 5 min. After the addition of Expand high-fidelity PCR system DNA polymerase ( Taq and Pwo DNA polymerases) (2.5 U; Roche Diagnostics), 30 cycles of 2 min at 72°C, 1 min at 94°C, and 2 min at 60°C (HuCV-OC43 N), 50°C (GAPDH), or 37°C (HuCV-OC43 S1) were performed, with a final elongation step of 10 min at 72°C. .. The DNA amplicons were separated by electrophoresis in a 1.5% (wt/vol) agarose gel with ethidium bromide (1 μg/ml).

Polymerase Chain Reaction:

Article Title: First Detection and Genotyping of Human-Associated Microsporidia in Pigeons from Urban Parks
Article Snippet: .. PCR amplification was done by using a 25-μl reaction mix containing 50 ng of DNA, 0.2 mM of each deoxynucleoside triphosphate, 0.2 μM each of the A and B primers, 1 unit of Taq DNA polymerase (Roche Molecular Biochemicals), and the PCR buffer, including a final concentration of 1.5 mM MgCl2 . ..

Article Title: Acute and Persistent Infection of Human Neural Cell Lines by Human Coronavirus OC43
Article Snippet: .. For PCR, 1 of 10 or 1 of 5 of the synthesized cDNAs was incubated in the presence of 20 pmol (HuCV-OC43 S1 and N) or 50 pmol (GAPDH) of the sense and antisense primers, 1.5 mM (HuCV-OC43 S1 or GAPDH) or 2.5 mM (HuCV-OC43 N) MgCl2 (Roche Diagnostics), 1× PCR buffer (10 mM Tris-HCl [pH 8.3], 50 mM KCl; Roche Diagnostics) and 0.4 mM (each) deoxynucleoside triphosphate (Li salt; Roche Diagnostics) at 94°C for 5 min and at 60°C (HuCV-OC43 N), 50°C (GAPDH), or 37°C (HuCV-OC43 S1) for another 5 min. After the addition of Expand high-fidelity PCR system DNA polymerase ( Taq and Pwo DNA polymerases) (2.5 U; Roche Diagnostics), 30 cycles of 2 min at 72°C, 1 min at 94°C, and 2 min at 60°C (HuCV-OC43 N), 50°C (GAPDH), or 37°C (HuCV-OC43 S1) were performed, with a final elongation step of 10 min at 72°C. .. The DNA amplicons were separated by electrophoresis in a 1.5% (wt/vol) agarose gel with ethidium bromide (1 μg/ml).

Article Title: Detection of Fungal DNA in Human Body Fluids and Tissues during a Multistate Outbreak of Fungal Meningitis and Other Infections
Article Snippet: .. PCRs were performed in 25-μl volumes and contained 2.5 μl of template DNA, 1× PCR buffer (20 mM Tris-HCl [pH 8.4], 50 mM KCl), 0.2 mM each of deoxynucleoside triphosphate, 1.6 mM MgCl2 , 0.2 pmol of each primer, and 0.6 U of Taq DNA polymerase (Roche Diagnostics, Indianapolis, IN). .. The following PCR conditions were used: 4 min at 94°C, 40 cycles consisting of a denaturing step at 94°C for 1 min, an annealing step at 52°C for 1 min, and a polymerization step at 72°C for 1 min, followed by a single incubation step for 2 min at 72°C.

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    Roche deoxynucleotide triphosphate
    Deoxynucleotide Triphosphate, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleotide triphosphate/product/Roche
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    Price from $9.99 to $1999.99
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