deoxynucleoside triphosphate  (Roche)


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    Structured Review

    Roche deoxynucleoside triphosphate
    Deoxynucleoside Triphosphate, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 196 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphate/product/Roche
    Average 92 stars, based on 196 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphate - by Bioz Stars, 2020-09
    92/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: First Detection and Genotyping of Human-Associated Microsporidia in Pigeons from Urban Parks
    Article Snippet: .. PCR amplification was done by using a 25-μl reaction mix containing 50 ng of DNA, 0.2 mM of each deoxynucleoside triphosphate, 0.2 μM each of the A and B primers, 1 unit of Taq DNA polymerase (Roche Molecular Biochemicals), and the PCR buffer, including a final concentration of 1.5 mM MgCl2 . ..

    Article Title: Cytotoxicity of Mycoplasma mycoides subsp. mycoides Small Colony Type to Bovine Epithelial Cells ▿
    Article Snippet: .. PCR was performed using a Gene Amp 9600 DNA thermal cycler (Applied Biosystems) with a 50-μl reaction mixture [50 mM Tris-HCl, pH 9.2, 1.75 mM MgCl2 , 16 mM (NH4 )2 SO4 , and 350 μM of each deoxynucleoside triphosphate] containing 300 nM of each primer (Table ), 1.75 U of Taq DNA polymerase (Roche Diagnostics), and approximately 50 ng of genomic DNA. .. The mixtures were subjected to 2 min of denaturation at 94°C, followed by 35 cycles of amplification with the following parameters: 30 s at 94°C, 30 s at 48°C, and 1.5 min of extension at 72°C for fragments of 1.2 kb or 2.5 min of extension at 72°C for fragments of 2.5 kb.

    Article Title: Mitochondrial Haplogroup N9a Confers Resistance against Type 2 Diabetes in Asians
    Article Snippet: .. The reaction mixture (25 μl) contained 1 ng of genomic DNA, 5 pmol of each primer, 0.2 mmol/liter of each deoxynucleoside triphosphate, 2 mmol/liter MgCl2 , and 1 U of DNA polymerase (FastStart Taq DNA Polymerase [Roche Diagnostics]) in the PCR buffer supplied by the manufacturer. .. The amplification protocol consisted of an initial denaturation at 95°C for 10 min followed by 40 cycles of denaturation at 94°C for 20 s, annealing at 60°C for 30 s, and extension at 72°C for 30 s, with a final extension at 72°C for 7 min.

    Article Title: Simple Method for Determination of the Number of Helicobacter pylori CagA Variable-Region EPIYA Tyrosine Phosphorylation Motifs by PCR
    Article Snippet: .. A reaction mixture containing 0.2 mM concentrations of each deoxynucleoside triphosphate, a 0.4 nM concentration of the forward primer, a 0.08 nM concentration of the reverse primer, 0.05 U of Taq DNA polymerase (Roche Diagnostics, Penzberg, Germany)/μl, and 1 μl of genomic DNA in buffer (10 mM Tris-HCl [pH 8.3], 1.5 mM magnesium chloride, 50 mM potassium chloride) was incubated at 95°C for 90 s, followed by 35 cycles at 95°C for 30 s, 57°C for 60 s, and 72°C for 30 s and a final extension at 72°C for 5 min. PCR products were separated on a 1.5% (wt/vol) agarose gel. .. CagA proteins differ in size due to the presence of repeat regions containing EPIYA motifs, and we have previously shown that H. pylori strains isolated from South Africa possess three to six TPMs within this variable region ( ).

    Concentration Assay:

    Article Title: First Detection and Genotyping of Human-Associated Microsporidia in Pigeons from Urban Parks
    Article Snippet: .. PCR amplification was done by using a 25-μl reaction mix containing 50 ng of DNA, 0.2 mM of each deoxynucleoside triphosphate, 0.2 μM each of the A and B primers, 1 unit of Taq DNA polymerase (Roche Molecular Biochemicals), and the PCR buffer, including a final concentration of 1.5 mM MgCl2 . ..

    Article Title: Simple Method for Determination of the Number of Helicobacter pylori CagA Variable-Region EPIYA Tyrosine Phosphorylation Motifs by PCR
    Article Snippet: .. A reaction mixture containing 0.2 mM concentrations of each deoxynucleoside triphosphate, a 0.4 nM concentration of the forward primer, a 0.08 nM concentration of the reverse primer, 0.05 U of Taq DNA polymerase (Roche Diagnostics, Penzberg, Germany)/μl, and 1 μl of genomic DNA in buffer (10 mM Tris-HCl [pH 8.3], 1.5 mM magnesium chloride, 50 mM potassium chloride) was incubated at 95°C for 90 s, followed by 35 cycles at 95°C for 30 s, 57°C for 60 s, and 72°C for 30 s and a final extension at 72°C for 5 min. PCR products were separated on a 1.5% (wt/vol) agarose gel. .. CagA proteins differ in size due to the presence of repeat regions containing EPIYA motifs, and we have previously shown that H. pylori strains isolated from South Africa possess three to six TPMs within this variable region ( ).

    Article Title: Detection of Carp Interstitial Nephritis and Gill Necrosis Virus in Fish Droppings
    Article Snippet: .. DNA preparations (20 to 50 ng) were used as templates in reaction mixtures containing 0.5 μM forward primer, 0.5 μM reverse primer, 1 mM MgCl2 , each of deoxynucleoside triphosphate at a concentration of 0.1 mM, 1 U of FastStart Taq DNA polymerase (Roche), and 10× reaction buffer containing 500 mM Tris-HCl, 100 mM KCl, 50 mM (NH4 )2 SO4 (pH 8.3) (Roche). .. Cycling was performed as follows: 10 min at 95°C and then 30 cycles of 94°C for 45 s, 55°C for 30 s, and 72°C for 45 s. PCR products were resolved on a 1% (wt/vol) agarose gel in 0.5× TAE (40 mM Tris-acetate, 1 mM EDTA).

    Article Title: Reversible Inhibition of Spreading of In Vitro Infection and Imbalance of Viral Protein Accumulation at Low pH in Viral Hemorrhagic Septicemia Rhabdovirus, a Salmonid Rhabdovirus
    Article Snippet: .. Reaction samples (50 μl) contained a 200 μM concentration of each deoxynucleoside triphosphate, a 0.1 μM concentration of the 5′ and 3′ primers, and 5 U of Taq DNA polymerase (Roche, Mannheim, Germany). .. Temperature profiles of the PCR assays consisted of 30 cycles of denaturation for 1 min at 94°C, annealing for 1 min at 52°C, and elongation for 2 min at 72°C.

    Incubation:

    Article Title: Simple Method for Determination of the Number of Helicobacter pylori CagA Variable-Region EPIYA Tyrosine Phosphorylation Motifs by PCR
    Article Snippet: .. A reaction mixture containing 0.2 mM concentrations of each deoxynucleoside triphosphate, a 0.4 nM concentration of the forward primer, a 0.08 nM concentration of the reverse primer, 0.05 U of Taq DNA polymerase (Roche Diagnostics, Penzberg, Germany)/μl, and 1 μl of genomic DNA in buffer (10 mM Tris-HCl [pH 8.3], 1.5 mM magnesium chloride, 50 mM potassium chloride) was incubated at 95°C for 90 s, followed by 35 cycles at 95°C for 30 s, 57°C for 60 s, and 72°C for 30 s and a final extension at 72°C for 5 min. PCR products were separated on a 1.5% (wt/vol) agarose gel. .. CagA proteins differ in size due to the presence of repeat regions containing EPIYA motifs, and we have previously shown that H. pylori strains isolated from South Africa possess three to six TPMs within this variable region ( ).

    Amplification:

    Article Title: First Detection and Genotyping of Human-Associated Microsporidia in Pigeons from Urban Parks
    Article Snippet: .. PCR amplification was done by using a 25-μl reaction mix containing 50 ng of DNA, 0.2 mM of each deoxynucleoside triphosphate, 0.2 μM each of the A and B primers, 1 unit of Taq DNA polymerase (Roche Molecular Biochemicals), and the PCR buffer, including a final concentration of 1.5 mM MgCl2 . ..

    Agarose Gel Electrophoresis:

    Article Title: Simple Method for Determination of the Number of Helicobacter pylori CagA Variable-Region EPIYA Tyrosine Phosphorylation Motifs by PCR
    Article Snippet: .. A reaction mixture containing 0.2 mM concentrations of each deoxynucleoside triphosphate, a 0.4 nM concentration of the forward primer, a 0.08 nM concentration of the reverse primer, 0.05 U of Taq DNA polymerase (Roche Diagnostics, Penzberg, Germany)/μl, and 1 μl of genomic DNA in buffer (10 mM Tris-HCl [pH 8.3], 1.5 mM magnesium chloride, 50 mM potassium chloride) was incubated at 95°C for 90 s, followed by 35 cycles at 95°C for 30 s, 57°C for 60 s, and 72°C for 30 s and a final extension at 72°C for 5 min. PCR products were separated on a 1.5% (wt/vol) agarose gel. .. CagA proteins differ in size due to the presence of repeat regions containing EPIYA motifs, and we have previously shown that H. pylori strains isolated from South Africa possess three to six TPMs within this variable region ( ).

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    Roche deoxynucleoside triphosphate
    Deoxynucleoside Triphosphate, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 211 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphate/product/Roche
    Average 92 stars, based on 211 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphate - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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