Structured Review

PerkinElmer deoxynucleoside triphosphate
Deoxynucleoside Triphosphate, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 263 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxynucleoside triphosphate/product/PerkinElmer
Average 92 stars, based on 263 article reviews
Price from $9.99 to $1999.99
deoxynucleoside triphosphate - by Bioz Stars, 2020-07
92/100 stars

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Polymerase Chain Reaction:

Article Title: Dose-Dependent Activation of Lymphocytes in Endotoxin-Induced Airway Inflammation
Article Snippet: .. Two microliters of cDNA was added to a 23-μl PCR mixture consisting of 1× GenAmp buffer (Perkin-Elmer, Norwalk, Conn.), 1.5 mM MgCl2 , a 0.2 mM concentration of each deoxynucleoside triphosphate, 0.4 μM sense and antisense primers, and 0.025 U of Taq polymerase (AmpliTaq Gold; Perkin-Elmer) per μl. .. The PCR profile used was the following: denaturation at 94°C for 30 s, annealing at 60°C for 30 s (annealing for IL-17 was at 50°C), and extension at 72°C for 60 s. The optimal number of amplification cycles for each primer pair was carefully monitored to ensure that the reaction was not run to completion (TNF-α, 28 cycles; IL-1α, 28 cycles; IL-1β, 26 to 28 cycles; IL-2, 35 cycles; IL-5, 32 cycles; IL-6, 30 cycles; IL-10, 35 cycles; IL-12, 30 cycles; IL-17, 35 cycles; IL-18, 24 cycles; IFN-γ, 30 cycles; RANTES, 22 cycles; MIP-1α, 26 cycles; MIP-2, 28 cycles; MCP-1, 28 cycles; MCP-3, 28 cycles; eotaxin, 26 cycles; IL-2Rα, 30 cycles; and GAPDH, 20 to 21 cycles).

Article Title: Hepatitis C Virus Genotyping: Interrogation of the 5? Untranslated Region Cannot Accurately Distinguish Genotypes 1a and 1b
Article Snippet: .. PCR amplification was performed with the primers described below in a model 9600 thermocycler (Perkin-Elmer) with 10 μl of cDNA in a total reaction volume of 50 μl containing each primer at 10 pmol, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , each deoxynucleoside triphosphate at a concentration of 200 μM, and 1.0 U of Taq DNA polymerase (Perkin-Elmer). .. After an initial denaturation step at 95°C for 1 min, 40 cycles of PCR at 95°C for 20 s, 56°C for 30 s, and 72°C for 1 min were carried out, with a final extension of 72°C for 10 min. Nested PCR of the NS-5b region was performed with 1 μl of the first-round amplification product if the amplification product was not sufficient for sequence analysis.

Article Title: PCR-Based DNA Amplification and Presumptive Detection of Escherichia coli O157:H7 with an Internal Fluorogenic Probe and the 5? Nuclease (TaqMan) Assay †
Article Snippet: .. Briefly, 5 μl of sample containing the DNA template to be evaluated was added to 45 μl of PCR master mix (5 μl of 1× PCR buffer II [Perkin-Elmer], 1.5 to 4.0 mM MgCl2 , 200 nM each primer [SZ-I and SZ-II], 200 μM deoxynucleoside triphosphate, 0.025 U of AmpliTaq DNA polymerase [Perkin-Elmer], 25 to 50 nM fluorogenic probe, 26 μl of water) in 200-μl capacity MOT or in individual wells of a MORP. ..

Article Title: Molecular Characterization and Identification of Biocontrol Isolates of Trichoderma spp.
Article Snippet: .. PCR amplifications were performed in a total volume of 50 μl by mixing 40 ng of the template DNA with 0.2 μM concentrations of each primer, 200 μM concentrations of each deoxynucleoside triphosphate, and 2.5 U of Taq DNA polymerase in GeneAmp 10× PCR Buffer II (100 mM Tris-HCl, pH 8.3; 500 mM KCl) (Perkin-Elmer). .. These reactions were subjected to an initial denaturation of 5 min at 95°C, followed by 35 cycles of 1.5 min at 94°C, 2 min at 55°C, and 3 min at 72°C, with a final extension of 5 min at 72°C in a Perkin-Elmer Cetus thermal cycler.

Concentration Assay:

Article Title: Dose-Dependent Activation of Lymphocytes in Endotoxin-Induced Airway Inflammation
Article Snippet: .. Two microliters of cDNA was added to a 23-μl PCR mixture consisting of 1× GenAmp buffer (Perkin-Elmer, Norwalk, Conn.), 1.5 mM MgCl2 , a 0.2 mM concentration of each deoxynucleoside triphosphate, 0.4 μM sense and antisense primers, and 0.025 U of Taq polymerase (AmpliTaq Gold; Perkin-Elmer) per μl. .. The PCR profile used was the following: denaturation at 94°C for 30 s, annealing at 60°C for 30 s (annealing for IL-17 was at 50°C), and extension at 72°C for 60 s. The optimal number of amplification cycles for each primer pair was carefully monitored to ensure that the reaction was not run to completion (TNF-α, 28 cycles; IL-1α, 28 cycles; IL-1β, 26 to 28 cycles; IL-2, 35 cycles; IL-5, 32 cycles; IL-6, 30 cycles; IL-10, 35 cycles; IL-12, 30 cycles; IL-17, 35 cycles; IL-18, 24 cycles; IFN-γ, 30 cycles; RANTES, 22 cycles; MIP-1α, 26 cycles; MIP-2, 28 cycles; MCP-1, 28 cycles; MCP-3, 28 cycles; eotaxin, 26 cycles; IL-2Rα, 30 cycles; and GAPDH, 20 to 21 cycles).

Article Title: Hepatitis C Virus Genotyping: Interrogation of the 5? Untranslated Region Cannot Accurately Distinguish Genotypes 1a and 1b
Article Snippet: .. PCR amplification was performed with the primers described below in a model 9600 thermocycler (Perkin-Elmer) with 10 μl of cDNA in a total reaction volume of 50 μl containing each primer at 10 pmol, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , each deoxynucleoside triphosphate at a concentration of 200 μM, and 1.0 U of Taq DNA polymerase (Perkin-Elmer). .. After an initial denaturation step at 95°C for 1 min, 40 cycles of PCR at 95°C for 20 s, 56°C for 30 s, and 72°C for 1 min were carried out, with a final extension of 72°C for 10 min. Nested PCR of the NS-5b region was performed with 1 μl of the first-round amplification product if the amplification product was not sufficient for sequence analysis.

Article Title: Possible Horizontal Transfer of the vanB2 Gene among Genetically Diverse Strains of Vancomycin-Resistant Enterococcus faecium in a Korean Hospital
Article Snippet: .. The reactions were carried out in a 100-μl reaction volume including 1× Tricine buffer, 1.5 mM Mg acetate, a 0.2 mM concentration of each deoxynucleoside triphosphate, a 0.25 μM concentration of each primer, 2 U of r Tth DNA polymerase (Perkin-Elmer, Norwalk, Conn.) and 1 μg of bacterial DNA. .. Using hot-start PCR, amplification conditions were 94°C for 1 min; 94°C for 15 s, and 68°C for 6 min for 16 cycles; 94°C for 15 s and 68°C for 6 min (with increments of 15 s per cycle for the 68°C step only) for 12 cycles; and a final 15-min extension period at 72°C.

Article Title: Adhesion-Aggregation and Inactivation of Poliovirus 1 in Groundwater Stored in a Hydrophobic Container
Article Snippet: .. cDNA was synthesized from the extracted RNA by use of the reverse primer (Ent-r) at a final concentration of 0.5 μM in a mix of 20 μl containing 4 μl of 5× reverse transcription (RT) buffer (250 mM Tris-HCl [pH 8.4], 50 mM MgCl2 , 350 mM KCl, 15 mM dithiothreitol, 2.5 mM spermidine), 40 U of RNase inhibitor (catalog no. N2111; Promega), 0.25 μM of each deoxynucleoside triphosphate (catalog no. N8080260; Perkin Elmer), 10 U of reverse transcriptase (catalog no. M5108; Promega), 6 μl of free DNase, RNase water (catalog no. W4502; Sigma), and 5 μl of extracted product heated 3 min at 95°C. .. RT was performed at 42°C for 60 min. RNA-DNA hybrids were denatured, and reverse transcriptase was inactivated by heating to 95°C for 5 min.

Article Title: Development of a Novel, Rapid Integrated Cryptosporidium parvum Detection Assay
Article Snippet: .. The reagents used in the nested set reaction mixture were (final concentrations) 1× EZ buffer (Perkin-Elmer, Foster City, Calif.), 1.25 mM manganese acetate, each deoxynucleoside triphosphate at a concentration of 160 μM, 500 nM CPOP716F, 500 nM CPOP992R, 100 nM CPOP805F-FITC, 100 nM CPOP948R-Biotin, and 5 U of rTth polymerase (Perkin-Elmer). .. The following PCR thermal profile was used: 95°C for 1 min; 60°C for 15 min; 40 cycles consisting of 95°C for 15 s and 65°C for 1 min; and then five cycles consisting of 95°C for 1 s and 55°C for 1 min.

Amplification:

Article Title: Hepatitis C Virus Genotyping: Interrogation of the 5? Untranslated Region Cannot Accurately Distinguish Genotypes 1a and 1b
Article Snippet: .. PCR amplification was performed with the primers described below in a model 9600 thermocycler (Perkin-Elmer) with 10 μl of cDNA in a total reaction volume of 50 μl containing each primer at 10 pmol, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , each deoxynucleoside triphosphate at a concentration of 200 μM, and 1.0 U of Taq DNA polymerase (Perkin-Elmer). .. After an initial denaturation step at 95°C for 1 min, 40 cycles of PCR at 95°C for 20 s, 56°C for 30 s, and 72°C for 1 min were carried out, with a final extension of 72°C for 10 min. Nested PCR of the NS-5b region was performed with 1 μl of the first-round amplification product if the amplification product was not sufficient for sequence analysis.

Synthesized:

Article Title: Adhesion-Aggregation and Inactivation of Poliovirus 1 in Groundwater Stored in a Hydrophobic Container
Article Snippet: .. cDNA was synthesized from the extracted RNA by use of the reverse primer (Ent-r) at a final concentration of 0.5 μM in a mix of 20 μl containing 4 μl of 5× reverse transcription (RT) buffer (250 mM Tris-HCl [pH 8.4], 50 mM MgCl2 , 350 mM KCl, 15 mM dithiothreitol, 2.5 mM spermidine), 40 U of RNase inhibitor (catalog no. N2111; Promega), 0.25 μM of each deoxynucleoside triphosphate (catalog no. N8080260; Perkin Elmer), 10 U of reverse transcriptase (catalog no. M5108; Promega), 6 μl of free DNase, RNase water (catalog no. W4502; Sigma), and 5 μl of extracted product heated 3 min at 95°C. .. RT was performed at 42°C for 60 min. RNA-DNA hybrids were denatured, and reverse transcriptase was inactivated by heating to 95°C for 5 min.

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  • 92
    PerkinElmer deoxynucleoside triphosphate mix
    Deoxynucleoside Triphosphate Mix, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 284 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphate mix/product/PerkinElmer
    Average 92 stars, based on 284 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphate mix - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    PerkinElmer deoxynucleotide triphosphate
    Deoxynucleotide Triphosphate, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleotide triphosphate/product/PerkinElmer
    Average 92 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    deoxynucleotide triphosphate - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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