Structured Review

Meridian Life Science deoxynucleoside triphosphate
Deoxynucleoside Triphosphate, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxynucleoside triphosphate/product/Meridian Life Science
Average 93 stars, based on 17 article reviews
Price from $9.99 to $1999.99
deoxynucleoside triphosphate - by Bioz Stars, 2020-07
93/100 stars

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Polymerase Chain Reaction:

Article Title: Molecular Epidemiology of Campylobacter coli Strains Isolated from Different Sources in New Zealand between 2005 and 2014
Article Snippet: .. Amplification was performed in a 20-μl reaction volume containing 0.2 μl of Platinum Taq polymerase (Invitrogen, USA), 2 μl of 10× PCR buffer (Invitrogen), 1 μl of deoxynucleoside triphosphate (dNTP; Bioline, United Kingdom) (2 mM), 0.6 μl of MgCl2 , 2 μl of each forward and reverse primer (2 pmol/μl), 6.2 μl of H2 O, and 2 μl of DNA. .. The reactions were carried out in a thermocycler (SensoQuest, Germany) with denaturation at 96°C for 2 min, followed by 35 cycles of 96°C for 30 s, 58°C for 30 s, and 72°C for 30 s and a final extension of 72°C for 2 min. Electrophoresis in a 1% agarose gel plus staining with ethidium bromide was used to visualize PCR products.

Article Title: Bacterial Origin and Community Composition in the Barley Phytosphere as a Function of Habitat and Presowing Conditions
Article Snippet: .. Each PCR mixture contained 10 to 50 ng of DNA template, a 0.4 μM concentration of each primer, a 250 μM concentration of each deoxynucleoside triphosphate, 5 μl of reaction buffer (10×; pH 8.8; Bioline, London, United Kingdom), 1.5 mM MgCl2 , 0.4 mg of bovine serum albumin ml−1 , 1 U of BIOTAQ polymerase (Bioline), and sterile MilliQ water to 50 μl. .. Thirty amplification cycles were carried out as follows: one cycle consisting of an initial denaturation at 95°C for 5 min followed by primer annealing at 50°C for 30 s and primer extension at 72°C for 30 s; 29 cycles of 94°C (92°C for the final 15 cycles) for 30 s, 50°C for 30 s, and 72°C for 45 s; and a final step consisting of 10 min of incubation at 72°C.

Article Title: Roseobacter-Like Bacteria in Red and Mediterranean Sea Aerobic Anoxygenic Photosynthetic Populations
Article Snippet: .. PCR amplification was carried out in a total volume of 25 μl containing 10 ng of template DNA, a 200 μM concentration of each deoxynucleoside triphosphate, 1.5 mM MgCl2 , 2.5 U of BIO-X-ACT DNA polymerase (Bioline) or Ex-Taq (Takara), and a 0.2 μM concentration of each primer. ..

Article Title: Molecular detection of bacteria in plant tissues, using universal 16S ribosomal DNA degenerated primers
Article Snippet: .. PCR amplification The PCR mixtures (50 μL) contained 5 μL of 10× reaction buffer [16 mmol/L (NH4 )2 SO4 ; 70 mmol/L Tris–HCl pH 8.8; 0.1% Tween 20]; 0.06 μL of each deoxynucleoside triphosphate (100 mmol/L); 0.15 μL of BioTaq (5 u/μL; Thermus aquaticus ; Bioline); 1.5 μL MgCl2 (50 mmol/L); 2 μL of each degenerated primer (30 pmol/L); 1 μL template DNA (approximately 10–40 ng); and 38.11 μL of analytical grade water. .. PCR amplification was performed with a thermal cycler (GenAmp In Situ PCR System 1000, PerkinElmer Cetus); cycles consisted of 6 min denaturation at 96 °C followed by 40 cycles of 30 s at 95 °C for further denaturation, 15 s at 59 °C (for DΣβ) primer set annealing, 1 min at 72 °C extension and ended with a 10 min extension at 72 °C.

Article Title: Use of Laser Microdissection for Phylogenetic Characterization of Polyphosphate-Accumulating Bacteria
Article Snippet: .. The reaction mixture contained 250 nM of each primer, 0.5 μg of bovine serum albumin (Roth, Karlsruhe, Germany), a 200 μM concentration of each deoxynucleoside triphosphate (Bioline GmbH, Luckenwalde, Germany), 2 mM MgCl2 , 1 μl of 10× PCR buffer, and 0.2 U HotStarTaq DNA polymerase (Qiagen, Hilden, Germany). ..

Article Title: Distribution of Tetracycline Resistance Genes in Actinobacillus pleuropneumoniae Isolates from Spain †
Article Snippet: .. The PCR mixture consisted of 10 μl of DNA template (10 pg/μl), 4 U of Taq DNA polymerase (Bioline, London, United Kingdom), 1.5 mM MgCl2 , 10 μl of 10× PCR amplification buffer [160 mM (NH4 )2 SO4 , 670 mM Tris-HCl (pH 8.8), 0.1% Tween 20], 40 pmol of each primer, 0.2 mM each deoxynucleoside triphosphate (Bioline, London, United Kingdom), and double-distilled water to a final volume of 100 μl. .. DNA was first denatured at 95°C for 5 min and then subjected to 30 cycles of amplification under the following conditions: denaturation at 95°C for 1 min, annealing for 1 min, and extension at 72°C for 1 min. After the final cycle, the reactions were terminated by an extra run at 72°C for 10 min, and the reaction products were then kept at 4°C until analysis.

Concentration Assay:

Article Title: Bacterial Origin and Community Composition in the Barley Phytosphere as a Function of Habitat and Presowing Conditions
Article Snippet: .. Each PCR mixture contained 10 to 50 ng of DNA template, a 0.4 μM concentration of each primer, a 250 μM concentration of each deoxynucleoside triphosphate, 5 μl of reaction buffer (10×; pH 8.8; Bioline, London, United Kingdom), 1.5 mM MgCl2 , 0.4 mg of bovine serum albumin ml−1 , 1 U of BIOTAQ polymerase (Bioline), and sterile MilliQ water to 50 μl. .. Thirty amplification cycles were carried out as follows: one cycle consisting of an initial denaturation at 95°C for 5 min followed by primer annealing at 50°C for 30 s and primer extension at 72°C for 30 s; 29 cycles of 94°C (92°C for the final 15 cycles) for 30 s, 50°C for 30 s, and 72°C for 45 s; and a final step consisting of 10 min of incubation at 72°C.

Article Title: Roseobacter-Like Bacteria in Red and Mediterranean Sea Aerobic Anoxygenic Photosynthetic Populations
Article Snippet: .. PCR amplification was carried out in a total volume of 25 μl containing 10 ng of template DNA, a 200 μM concentration of each deoxynucleoside triphosphate, 1.5 mM MgCl2 , 2.5 U of BIO-X-ACT DNA polymerase (Bioline) or Ex-Taq (Takara), and a 0.2 μM concentration of each primer. ..

Article Title: Use of Laser Microdissection for Phylogenetic Characterization of Polyphosphate-Accumulating Bacteria
Article Snippet: .. The reaction mixture contained 250 nM of each primer, 0.5 μg of bovine serum albumin (Roth, Karlsruhe, Germany), a 200 μM concentration of each deoxynucleoside triphosphate (Bioline GmbH, Luckenwalde, Germany), 2 mM MgCl2 , 1 μl of 10× PCR buffer, and 0.2 U HotStarTaq DNA polymerase (Qiagen, Hilden, Germany). ..

Activated Clotting Time Assay:

Article Title: Development of Three PCR Assays for the Differentiation between Echinococcus shiquicus, E. granulosus (G1 genotype), and E. multilocularis DNA in the Co-Endemic Region of Qinghai-Tibet plateau, China
Article Snippet: .. Amplification was performed in a 50 μL reaction volume with 5× manufacturers Flexi reaction buffer (Promega Ltd.), 200 μM of each deoxynucleoside triphosphate (dNTPs; Bioline), 0.3 μM of each primer (Eg1F81, 5′ GTT TTT GGC TGC CGC CAG AAC 3′ and Eg1R83, 5′ AAT TAA TGG AAA TAA TAA CAA ACT TAA TCA ACA AT 3′), 2 mM MgCl2 and 2.5 U GoTaq polymerase (Promega Ltd.). .. The mastermix was overlaid with mineral oil and the reaction was subjected to an initial incubation of 5 min at 94°C for 1 cycle, followed by 36 cycles each consisting of 30 s at 94°C, 50 s at 62°C, and 30 s at 72°C to amplify a species-specific 226 bp fragment.

Amplification:

Article Title: Molecular Epidemiology of Campylobacter coli Strains Isolated from Different Sources in New Zealand between 2005 and 2014
Article Snippet: .. Amplification was performed in a 20-μl reaction volume containing 0.2 μl of Platinum Taq polymerase (Invitrogen, USA), 2 μl of 10× PCR buffer (Invitrogen), 1 μl of deoxynucleoside triphosphate (dNTP; Bioline, United Kingdom) (2 mM), 0.6 μl of MgCl2 , 2 μl of each forward and reverse primer (2 pmol/μl), 6.2 μl of H2 O, and 2 μl of DNA. .. The reactions were carried out in a thermocycler (SensoQuest, Germany) with denaturation at 96°C for 2 min, followed by 35 cycles of 96°C for 30 s, 58°C for 30 s, and 72°C for 30 s and a final extension of 72°C for 2 min. Electrophoresis in a 1% agarose gel plus staining with ethidium bromide was used to visualize PCR products.

Article Title: Development of Three PCR Assays for the Differentiation between Echinococcus shiquicus, E. granulosus (G1 genotype), and E. multilocularis DNA in the Co-Endemic Region of Qinghai-Tibet plateau, China
Article Snippet: .. Amplification was performed in a 50 μL reaction volume with 5× manufacturers Flexi reaction buffer (Promega Ltd.), 200 μM of each deoxynucleoside triphosphate (dNTPs; Bioline), 0.3 μM of each primer (Eg1F81, 5′ GTT TTT GGC TGC CGC CAG AAC 3′ and Eg1R83, 5′ AAT TAA TGG AAA TAA TAA CAA ACT TAA TCA ACA AT 3′), 2 mM MgCl2 and 2.5 U GoTaq polymerase (Promega Ltd.). .. The mastermix was overlaid with mineral oil and the reaction was subjected to an initial incubation of 5 min at 94°C for 1 cycle, followed by 36 cycles each consisting of 30 s at 94°C, 50 s at 62°C, and 30 s at 72°C to amplify a species-specific 226 bp fragment.

Article Title: Roseobacter-Like Bacteria in Red and Mediterranean Sea Aerobic Anoxygenic Photosynthetic Populations
Article Snippet: .. PCR amplification was carried out in a total volume of 25 μl containing 10 ng of template DNA, a 200 μM concentration of each deoxynucleoside triphosphate, 1.5 mM MgCl2 , 2.5 U of BIO-X-ACT DNA polymerase (Bioline) or Ex-Taq (Takara), and a 0.2 μM concentration of each primer. ..

Article Title: Molecular detection of bacteria in plant tissues, using universal 16S ribosomal DNA degenerated primers
Article Snippet: .. PCR amplification The PCR mixtures (50 μL) contained 5 μL of 10× reaction buffer [16 mmol/L (NH4 )2 SO4 ; 70 mmol/L Tris–HCl pH 8.8; 0.1% Tween 20]; 0.06 μL of each deoxynucleoside triphosphate (100 mmol/L); 0.15 μL of BioTaq (5 u/μL; Thermus aquaticus ; Bioline); 1.5 μL MgCl2 (50 mmol/L); 2 μL of each degenerated primer (30 pmol/L); 1 μL template DNA (approximately 10–40 ng); and 38.11 μL of analytical grade water. .. PCR amplification was performed with a thermal cycler (GenAmp In Situ PCR System 1000, PerkinElmer Cetus); cycles consisted of 6 min denaturation at 96 °C followed by 40 cycles of 30 s at 95 °C for further denaturation, 15 s at 59 °C (for DΣβ) primer set annealing, 1 min at 72 °C extension and ended with a 10 min extension at 72 °C.

Article Title: Distribution of Tetracycline Resistance Genes in Actinobacillus pleuropneumoniae Isolates from Spain †
Article Snippet: .. The PCR mixture consisted of 10 μl of DNA template (10 pg/μl), 4 U of Taq DNA polymerase (Bioline, London, United Kingdom), 1.5 mM MgCl2 , 10 μl of 10× PCR amplification buffer [160 mM (NH4 )2 SO4 , 670 mM Tris-HCl (pH 8.8), 0.1% Tween 20], 40 pmol of each primer, 0.2 mM each deoxynucleoside triphosphate (Bioline, London, United Kingdom), and double-distilled water to a final volume of 100 μl. .. DNA was first denatured at 95°C for 5 min and then subjected to 30 cycles of amplification under the following conditions: denaturation at 95°C for 1 min, annealing for 1 min, and extension at 72°C for 1 min. After the final cycle, the reactions were terminated by an extra run at 72°C for 10 min, and the reaction products were then kept at 4°C until analysis.

Cellular Antioxidant Activity Assay:

Article Title: Development of Three PCR Assays for the Differentiation between Echinococcus shiquicus, E. granulosus (G1 genotype), and E. multilocularis DNA in the Co-Endemic Region of Qinghai-Tibet plateau, China
Article Snippet: .. Amplification was performed in a 50 μL reaction volume with 5× manufacturers Flexi reaction buffer (Promega Ltd.), 200 μM of each deoxynucleoside triphosphate (dNTPs; Bioline), 0.3 μM of each primer (Eg1F81, 5′ GTT TTT GGC TGC CGC CAG AAC 3′ and Eg1R83, 5′ AAT TAA TGG AAA TAA TAA CAA ACT TAA TCA ACA AT 3′), 2 mM MgCl2 and 2.5 U GoTaq polymerase (Promega Ltd.). .. The mastermix was overlaid with mineral oil and the reaction was subjected to an initial incubation of 5 min at 94°C for 1 cycle, followed by 36 cycles each consisting of 30 s at 94°C, 50 s at 62°C, and 30 s at 72°C to amplify a species-specific 226 bp fragment.