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Kaneka Corp deoxynucleoside triphosphate
Deoxynucleoside Triphosphate, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 92/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxynucleoside triphosphate/product/Kaneka Corp
Average 92 stars, based on 32 article reviews
Price from $9.99 to $1999.99
deoxynucleoside triphosphate - by Bioz Stars, 2020-07
92/100 stars

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RNA Extraction:

Article Title: Identification of Encephalomyocarditis Virus in Clinical Samples by Reverse Transcription-PCR Followed by Genetic Typing Using Sequence Analysis
Article Snippet: .. For each RT reaction, 3.8 μl of RNA extraction product was added to 6.2 μl of a RT premixture to obtain a final concentration of 1× first-strand buffer (Gibco BRL), a 0.5 mM each deoxynucleoside triphosphate (Eurogentec), 10 mM dithiothreitol (Gibco BRL), 100 U of Moloney murine leukemia virus reverse transcriptase (Gibco BRL), and 3.5 pmol of random hexamers (Pharmacia Biotech) per μl. ..

Amplification:

Article Title: Human Plasmodium knowlesi infections in young children in central Vietnam
Article Snippet: .. The nested PCR amplification was carried out in a 25 μl reaction mixture containing 1× reaction buffer (10× Qiagen Buffer, Hilden Germany), 3 mM MgCl2 (Qiagen, Hilden Germany), 200 mM of each deoxynucleoside triphosphate (Eurogentec, Belgium), 250 nM of each primers (Pmk8: 5'-GTT AGC GAG AGC CAC AAA AAA GCG AAT-3'; Pmk9r: 5'-ACT CAA AGT AAC AAA ATC TTC CGT A-3') and 2 U HotStarTaq Plus DNA polymerase (Qiagen, Hilden Germany) and 2 μl of the primary PCR products were used as DNA templates. .. All PCR reactions were carried out using PTC 100 thermal cycler (Bio-Rad, California USA).

Article Title: Shared Genotypes of Achromobacter xylosoxidans Strains Isolated from Patients at a Cystic Fibrosis Rehabilitation Center
Article Snippet: .. For the selective amplification of the restriction fragments, five microliters of the diluted restriction-ligation mixture was used for amplification in a volume of 10 μl under the following conditions: 0.4 μM TET-labeled EcoR + 0 primer, 1.2 μM Mse + C primer (Eurogentec, Seraing, Belgium) (E1), 0.2 mM each deoxynucleoside triphosphate, 1.5 mM MgCl2 , 1× reaction buffer, and 1 U of GoldStar DNA polymerase (Eurogentec). .. An overview of PCR primers and adapter sequences is shown in Table .

Article Title: Isolation of Adherent Polycyclic Aromatic Hydrocarbon (PAH)-Degrading Bacteria Using PAH-Sorbing Carriers
Article Snippet: .. PCR amplification was performed in a final volume of 50 μl in which 1 to 7 μl of genomic DNA solution was mixed with a reaction buffer (75 mM Tris HCl [pH 9.0], 20 mM (NH4 )2 SO4 , 0.01% [wt/vol] Tween 20), MgCl2 (2.5 mM), 1 μg of each primer, a 1 μM concentration of each deoxynucleoside triphosphate and 0.5 U of Red Goldstar polymerase (Eurogentec, Seraing, Belgium). ..

Cellular Antioxidant Activity Assay:

Article Title: Human Plasmodium knowlesi infections in young children in central Vietnam
Article Snippet: .. The nested PCR amplification was carried out in a 25 μl reaction mixture containing 1× reaction buffer (10× Qiagen Buffer, Hilden Germany), 3 mM MgCl2 (Qiagen, Hilden Germany), 200 mM of each deoxynucleoside triphosphate (Eurogentec, Belgium), 250 nM of each primers (Pmk8: 5'-GTT AGC GAG AGC CAC AAA AAA GCG AAT-3'; Pmk9r: 5'-ACT CAA AGT AAC AAA ATC TTC CGT A-3') and 2 U HotStarTaq Plus DNA polymerase (Qiagen, Hilden Germany) and 2 μl of the primary PCR products were used as DNA templates. .. All PCR reactions were carried out using PTC 100 thermal cycler (Bio-Rad, California USA).

Concentration Assay:

Article Title: Identification of Encephalomyocarditis Virus in Clinical Samples by Reverse Transcription-PCR Followed by Genetic Typing Using Sequence Analysis
Article Snippet: .. For each RT reaction, 3.8 μl of RNA extraction product was added to 6.2 μl of a RT premixture to obtain a final concentration of 1× first-strand buffer (Gibco BRL), a 0.5 mM each deoxynucleoside triphosphate (Eurogentec), 10 mM dithiothreitol (Gibco BRL), 100 U of Moloney murine leukemia virus reverse transcriptase (Gibco BRL), and 3.5 pmol of random hexamers (Pharmacia Biotech) per μl. ..

Article Title: Isolation of Adherent Polycyclic Aromatic Hydrocarbon (PAH)-Degrading Bacteria Using PAH-Sorbing Carriers
Article Snippet: .. PCR amplification was performed in a final volume of 50 μl in which 1 to 7 μl of genomic DNA solution was mixed with a reaction buffer (75 mM Tris HCl [pH 9.0], 20 mM (NH4 )2 SO4 , 0.01% [wt/vol] Tween 20), MgCl2 (2.5 mM), 1 μg of each primer, a 1 μM concentration of each deoxynucleoside triphosphate and 0.5 U of Red Goldstar polymerase (Eurogentec, Seraing, Belgium). ..

Polymerase Chain Reaction:

Article Title: Human Plasmodium knowlesi infections in young children in central Vietnam
Article Snippet: .. The nested PCR amplification was carried out in a 25 μl reaction mixture containing 1× reaction buffer (10× Qiagen Buffer, Hilden Germany), 3 mM MgCl2 (Qiagen, Hilden Germany), 200 mM of each deoxynucleoside triphosphate (Eurogentec, Belgium), 250 nM of each primers (Pmk8: 5'-GTT AGC GAG AGC CAC AAA AAA GCG AAT-3'; Pmk9r: 5'-ACT CAA AGT AAC AAA ATC TTC CGT A-3') and 2 U HotStarTaq Plus DNA polymerase (Qiagen, Hilden Germany) and 2 μl of the primary PCR products were used as DNA templates. .. All PCR reactions were carried out using PTC 100 thermal cycler (Bio-Rad, California USA).

Article Title: Practical Implementation of a Multiplex PCR for Acute Respiratory Tract Infections in Children
Article Snippet: .. Both the outer and the inner PCR mixtures contained 20 ng of each of the six appropriate primers, 200 μM deoxynucleoside triphosphate, 2.0 mM MgCl2 , 0.5 U of Taq polymerase, 2.5 μl of 10× Taq buffer (Silverstar; Eurogentec, Maastricht, The Netherlands), and water. .. To the outer PCR mixture, 5 μl of nucleic acid solution was added to obtain a final volume of 25 μl.

Article Title: Isolation of Adherent Polycyclic Aromatic Hydrocarbon (PAH)-Degrading Bacteria Using PAH-Sorbing Carriers
Article Snippet: .. PCR amplification was performed in a final volume of 50 μl in which 1 to 7 μl of genomic DNA solution was mixed with a reaction buffer (75 mM Tris HCl [pH 9.0], 20 mM (NH4 )2 SO4 , 0.01% [wt/vol] Tween 20), MgCl2 (2.5 mM), 1 μg of each primer, a 1 μM concentration of each deoxynucleoside triphosphate and 0.5 U of Red Goldstar polymerase (Eurogentec, Seraing, Belgium). ..

Nested PCR:

Article Title: Human Plasmodium knowlesi infections in young children in central Vietnam
Article Snippet: .. The nested PCR amplification was carried out in a 25 μl reaction mixture containing 1× reaction buffer (10× Qiagen Buffer, Hilden Germany), 3 mM MgCl2 (Qiagen, Hilden Germany), 200 mM of each deoxynucleoside triphosphate (Eurogentec, Belgium), 250 nM of each primers (Pmk8: 5'-GTT AGC GAG AGC CAC AAA AAA GCG AAT-3'; Pmk9r: 5'-ACT CAA AGT AAC AAA ATC TTC CGT A-3') and 2 U HotStarTaq Plus DNA polymerase (Qiagen, Hilden Germany) and 2 μl of the primary PCR products were used as DNA templates. .. All PCR reactions were carried out using PTC 100 thermal cycler (Bio-Rad, California USA).