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Fisher Scientific deoxynucleoside triphosphate
Deoxynucleoside Triphosphate, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxynucleoside triphosphate/product/Fisher Scientific
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
deoxynucleoside triphosphate - by Bioz Stars, 2020-07
92/100 stars

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Multiplex Assay:

Article Title: Simultaneous Detection of Marine Fish Pathogens by Using Multiplex PCR and a DNA Microarray
Article Snippet: .. Multiplex PCR mixtures (50-μl volume) each contained 50 to 100 ng of purified genomic DNA, 200 μM each deoxynucleoside triphosphate, 400 nM each primer, 2.5 mM MgCl2 , 1× reaction buffer, and 2 U of Taq polymerase (Fisher Scientific, Pittsburgh, Pa.). .. Thermal cycling was performed with a Mastercycler (Eppendorf, Hamburg, Germany) and included an initial incubation at 95°C for 3 min followed by 30 amplification cycles.

Incubation:

Article Title: Pax3-FKHR Knock-In Mice Show Developmental Aberrations but Do Not Develop Tumors
Article Snippet: .. RNA was mixed with 10 pmol of the specific primer (primer 5 for Pax3 RT, primer 7 for FKHR RT, and primer 10 for the β-actin [Table , Fig. ]) in a total volume of 12 μl, incubated at 65°C for 10 min, and chilled on ice for 5 min. Then 4 μl of 5× first-strand buffer (Life Technologies), 2 μl of 100 mM dithiothreitol (Promega), 1 μl each of 10 mM deoxynucleoside triphosphate (Fisher Scientific), 10 U of RNasin (Promega), and 200 U of Moloney murine leukemia virus RT (Life Technologies) were added. .. The reaction mixture was incubated at 37°C for 1 h, and the reaction was stopped by heat inactivation at 95°C for 5 min. A 2-μl aliquot of each reverse transcription reaction mixture was used for a subsequent PCR.

Purification:

Article Title: Simultaneous Detection of Marine Fish Pathogens by Using Multiplex PCR and a DNA Microarray
Article Snippet: .. Multiplex PCR mixtures (50-μl volume) each contained 50 to 100 ng of purified genomic DNA, 200 μM each deoxynucleoside triphosphate, 400 nM each primer, 2.5 mM MgCl2 , 1× reaction buffer, and 2 U of Taq polymerase (Fisher Scientific, Pittsburgh, Pa.). .. Thermal cycling was performed with a Mastercycler (Eppendorf, Hamburg, Germany) and included an initial incubation at 95°C for 3 min followed by 30 amplification cycles.

Concentration Assay:

Article Title: Isolation and Characterization of Endophytic Colonizing Bacteria from Agronomic Crops and Prairie Plants †
Article Snippet: .. Each reaction mixture contained Taq DNA polymerase (Promega, Madison, Wis.), 1.5 mM magnesium chloride, each deoxynucleoside triphosphate at a concentration of 0.1 mM, 10% (vol/vol) dimethyl sulfoxide (Fisher Scientific, Pittsburgh, Pa.), 0.4 mM spermidine (Sigma-Aldrich Co.), each primer (Integrated DNA Technologies, Coralville, Iowa) at a concentration of 10 pM, and 10 ng of DNA per μl. .. The thermocycling conditions consisted of a denaturation step at 94°C for 3 min, 30 amplification cycles of 94°C for 1 min, 57°C for 1 min, and 72°C for 2 min, and a final polymerization step of 72°C for 4 min with a GeneAmp PCR System 9600 (Perkin-Elmer, Norwalk, Conn.).

Generated:

Article Title: Phenotypic Switching in Candida glabrata Involves Phase-Specific Regulation of the Metallothionein Gene MT-II and the Newly Discovered Hemolysin Gene HLP
Article Snippet: .. PCR products were generated in 100 μl of a reaction mixture containing 10 mM buffer B (Fischer Scientific, St. Louis, Mo.), 1.2 mM MgCl2 , 100 μM deoxynucleoside triphosphate, 50 μM each 5′ primer and 3′ primer, and 2.5 U of Taq polymerase (Fischer Scientific). .. Conditions for PCR cycling of MT-I , MT-IIa , and AMT-I included 40 cycles of denaturation at 92°C for 1 min, annealing at 40°C for 1.5 min, and extension at 68°C for 1.5 min. To amplify SLFI , the annealing temperature was changed to 37°C.

Polymerase Chain Reaction:

Article Title: Simultaneous Detection of Marine Fish Pathogens by Using Multiplex PCR and a DNA Microarray
Article Snippet: .. Multiplex PCR mixtures (50-μl volume) each contained 50 to 100 ng of purified genomic DNA, 200 μM each deoxynucleoside triphosphate, 400 nM each primer, 2.5 mM MgCl2 , 1× reaction buffer, and 2 U of Taq polymerase (Fisher Scientific, Pittsburgh, Pa.). .. Thermal cycling was performed with a Mastercycler (Eppendorf, Hamburg, Germany) and included an initial incubation at 95°C for 3 min followed by 30 amplification cycles.

Article Title: Optimization of terminal restriction fragment polymorphism (TRFLP) analysis of human gut microbiota
Article Snippet: .. Each 50 μl reaction mixture contained 1X PCR buffer, 1.5 mM MgCl2 , 0.1 mM of each deoxynucleoside triphosphate, 0.2 μM forward and reverse primers, 1 U of Taq polymerase (Fisher Scientific), 0.5 mg/ml bovine serum albumin (New England BioLabs, Ipswich, MA) and approximately 200 ng genomic DNA. ..

Article Title: Validation of Mixed-Genome Microarrays as a Method for Genetic Discrimination ▿
Article Snippet: .. PCR was performed in 50-μl volumes with 10 μl of gDNA template (10 ng), 1× reaction buffer (Fisher Scientific, Pittsburgh, PA), 0.2 mM (each) deoxynucleoside triphosphate, 2 mM MgCl2 , 1 U Taq (Fisher Scientific), and 0.4 μM of each primer. ..

Article Title: Reduction of Escherichia coli O157:H7 Populations in Cattle by Addition of Colicin E7-Producing E. coli to Feed
Article Snippet: .. Two microliters of cell templates was mixed with 23 μl of PCR mixture containing 45 pmol of BOX A1R primer (5′-CTACGGCAAGGCGACGCTGACG-3′), 1.25 mM each deoxynucleoside triphosphate (Fisher Scientific, Pittsburgh, Pa.), 1 U of Taq DNA polymerase (Fisher Scientific), 5 μl of Gitschier buffer , 2.5 μl of dimethyl sulfoxide, and 160 μg of bovine serum albumin (Fisher Scientific)/ml. .. The PCR was performed with a Robocycler Gradient 96 Thermocycler (Stratagene, Inc., La Jolla, Calif.) for 2 min at 95°C, followed by 30 cycles of 93°C for 30 s, 50°C for 1 min, and 65°C for 8 min.

Article Title: Phenotypic Switching in Candida glabrata Involves Phase-Specific Regulation of the Metallothionein Gene MT-II and the Newly Discovered Hemolysin Gene HLP
Article Snippet: .. PCR products were generated in 100 μl of a reaction mixture containing 10 mM buffer B (Fischer Scientific, St. Louis, Mo.), 1.2 mM MgCl2 , 100 μM deoxynucleoside triphosphate, 50 μM each 5′ primer and 3′ primer, and 2.5 U of Taq polymerase (Fischer Scientific). .. Conditions for PCR cycling of MT-I , MT-IIa , and AMT-I included 40 cycles of denaturation at 92°C for 1 min, annealing at 40°C for 1.5 min, and extension at 68°C for 1.5 min. To amplify SLFI , the annealing temperature was changed to 37°C.

Article Title: Salmonella SPI-1-mediated neutrophil recruitment during enteric colitis is associated with reduction and alteration in intestinal microbiota
Article Snippet: .. Each 25 µL reaction mixture contained 1x PCR buffer, 2 mM MgCl2 , 0.4 mM of each of the deoxynucleoside triphosphate, 0.4 µM forward and reverse primers, 1.25 U of Taq polymerase (Fisher Scientific), 0.1 mg/ml bovine serum albumin and approximately 50 ng genomic DNA. ..

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    Fisher Scientific 2 deoxynucleoside 5 triphosphate
    2 Deoxynucleoside 5 Triphosphate, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2 deoxynucleoside 5 triphosphate/product/Fisher Scientific
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    2 deoxynucleoside 5 triphosphate - by Bioz Stars, 2020-07
    90/100 stars
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