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Eurobio deoxynucleoside triphosphate
Deoxynucleoside Triphosphate, supplied by Eurobio, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxynucleoside triphosphate/product/Eurobio
Average 92 stars, based on 17 article reviews
Price from $9.99 to $1999.99
deoxynucleoside triphosphate - by Bioz Stars, 2020-07
92/100 stars

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Polymerase Chain Reaction:

Article Title: Persistence of Mycoplasma hyopneumoniae in Experimentally Infected Pigs after Marbofloxacin Treatment and Detection of Mutations in the parC Gene
Article Snippet: .. PCR was performed with a GeneAmp PCR system 9700 thermal cycler (Applied Biosystems, Courtaboeuf, France) in a total volume of 50 μl containing 0.5 μM of each primer (Table ), 200 μM of each deoxynucleoside triphosphate, 5 μl of 10× Eurobio Taq buffer (Eurobio, Les Ulis, France), and 1 U of Eurobio Taq polymerase. .. Specific PCR products obtained for QRDRs were purified with a Qiaquick PCR purification kit (QIAGEN, Courtaboeuf, France) following manufacturer recommendations.

Article Title: In Vitro Activation of Feline Immunodeficiency Virus in Ramified Microglial Cells from Asymptomatically Infected Cats
Article Snippet: .. The 50-μl PCR mixtures consisted of 5 μl of RT product, 10 mM Tris HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , 200 μM concentrations of each deoxynucleoside triphosphate, 20 pmol of each outer primer, 0.2 U of inorganic pyrophosphatase (Eurobio, Les Ulis, France), and 2 U of Taq polymerase. .. After the initial denaturation step (2 min, 94°C), amplification was carried out by applying 40 cycles of 94°C for 1 min, 44°C for 1 min, and 72°C for 1.5 min and a final extension of 5 min at 72°C.

Article Title: Cyanophage Diversity, Inferred from g20 Gene Analyses, in the Largest Natural Lake in France, Lake Bourget
Article Snippet: .. The 50-μl reaction mix for PCR-DGGE contained 10× Taq reaction buffer (Eurobio), 2.5 mM MgCl2 , 200 μM concentrations of each deoxynucleoside triphosphate, 1 μM concentrations of each primer (CPS1GC and CPS8), 1.25 U of Taq DNA polymerase (Eurobluetaq; Eurobio), and 20 μl of viral lysate. ..

Article Title: Evaluation of FRET real-time PCR assay for rapid detection and differentiation of Plasmodium species in returning travellers and migrants
Article Snippet: .. The PCR reaction was realized in a final volume of 50 μl containing the following reagent mixture: 1× of PCR buffer 10× (500 mM KCl, 100 mM Tris-HCl [pH 8.3], 20 mM MgCl2 ), 5 mM concentration of MgCl2 , 0.5 μM each oligonucleotide primer, 200 μM deoxynucleoside triphosphate (Eurobio), and 0.02 unit Taq DNA polymerase (Eurobio). .. For each PCR tube, 40 μl PCR reagent mixture and 10 μl DNA were used.

Article Title: Reconstitution of Authentic Nanovirus from Multiple Cloned DNAs
Article Snippet: .. Candidate bacteria for harboring recombinant plasmids (i.e., forming white colonies on indicator plates) were boiled in 20 μl of H2 O, and 1 μl thereof was directly subjected to PCR in a total volume of 20 μl containing 10 pmol each of M13 direct and reverse sequencing primers, 67 mM Tris-HCl (pH 8.8), 16 mM (NH4 )2 SO4 , 0.01% Tween 20, 1.5 mM MgCl2 , 50 μM of each deoxynucleoside triphosphate, and 0.5 units of Taq II DNA polymerase (Eurobio). .. To avoid excessive redundant sequencing of FBNSV DNAs, additional PCRs (35 cycles of 95°C for 30 s, 53°C for 30 s, and 72°C for 90 s) using component-specific primers as specified in Table S1 in the supplemental material were run.

Article Title: Genetic Diversity of Small Eukaryotes in Lakes Differing by Their Trophic Status
Article Snippet: .. The PCR mixture (50 μl) contained about 10 ng of environmental DNA, 200 μM of each deoxynucleoside triphosphate, 2 mM MgCl2 , 10 pmol of each primer, 1.5 U of Taq DNA polymerase (Eurobio), and the PCR buffer supplied with the enzyme. .. Reactions were carried out in an automated thermocycler (MJ Research PTC 200-cycler) with the following cycle: initial denaturation at 95°C for 5 min; 30 cycles of denaturation at 95°C for 1 min, annealing at 57°C for 1 min, and extension at 72°C for 1 min, 30 s; and a final extension at 72°C for 10 min. Several PCR products (at least four 50-μl samples) were pooled, precipitated with ethanol-sodium acetate, and resuspended in 50 μl of sterile water.

Article Title: Development of a Multiple-Locus Variable-Number Tandem-Repeat Typing Scheme for Genetic Fingerprinting of Burkholderia cenocepacia and Application to Nationwide Epidemiological Analysis
Article Snippet: .. PCR was performed in a reaction volume of 25 μl containing 1× PCR buffer, 1.5 mM MgCl2 , 1 M betaine (Sigma-Aldrich, Saint-Quentin-Fallavier, France), 1 U of Taq DNA polymerase (Eurobio, Les Ulis, France), 200 μM each deoxynucleoside triphosphate (Eurobio), 0.2 μM each primer (Eurofins MWG Operon, Les Ulis, France), and 2.5 μl of DNA template. .. Amplification was carried out using a VWR Doppio thermal cycler, under the following conditions: initial denaturation at 96°C for 4 min, 30 cycles of denaturation at 96°C for 30 s, annealing at 60°C for 1 min, and elongation at 72°C for 1 min, and a final elongation step at 72°C for 5 min. One microliter of PCR product was separated in 2% (for BcenM01, BcenM02, BcenM03, BcenM04, BcenM07, and BcenM10), 1.5% (for BcenM05 and BcenM09), or 1% (BcenM06 and BcenM08) (wt/vol) agarose gels (Resophor; Eurobio) in Tris-borate-EDTA (TBE) buffer (Euromedex, Souffelweyersheim, France).

Concentration Assay:

Article Title: Evaluation of FRET real-time PCR assay for rapid detection and differentiation of Plasmodium species in returning travellers and migrants
Article Snippet: .. The PCR reaction was realized in a final volume of 50 μl containing the following reagent mixture: 1× of PCR buffer 10× (500 mM KCl, 100 mM Tris-HCl [pH 8.3], 20 mM MgCl2 ), 5 mM concentration of MgCl2 , 0.5 μM each oligonucleotide primer, 200 μM deoxynucleoside triphosphate (Eurobio), and 0.02 unit Taq DNA polymerase (Eurobio). .. For each PCR tube, 40 μl PCR reagent mixture and 10 μl DNA were used.

Recombinant:

Article Title: Reconstitution of Authentic Nanovirus from Multiple Cloned DNAs
Article Snippet: .. Candidate bacteria for harboring recombinant plasmids (i.e., forming white colonies on indicator plates) were boiled in 20 μl of H2 O, and 1 μl thereof was directly subjected to PCR in a total volume of 20 μl containing 10 pmol each of M13 direct and reverse sequencing primers, 67 mM Tris-HCl (pH 8.8), 16 mM (NH4 )2 SO4 , 0.01% Tween 20, 1.5 mM MgCl2 , 50 μM of each deoxynucleoside triphosphate, and 0.5 units of Taq II DNA polymerase (Eurobio). .. To avoid excessive redundant sequencing of FBNSV DNAs, additional PCRs (35 cycles of 95°C for 30 s, 53°C for 30 s, and 72°C for 90 s) using component-specific primers as specified in Table S1 in the supplemental material were run.

Sequencing:

Article Title: Reconstitution of Authentic Nanovirus from Multiple Cloned DNAs
Article Snippet: .. Candidate bacteria for harboring recombinant plasmids (i.e., forming white colonies on indicator plates) were boiled in 20 μl of H2 O, and 1 μl thereof was directly subjected to PCR in a total volume of 20 μl containing 10 pmol each of M13 direct and reverse sequencing primers, 67 mM Tris-HCl (pH 8.8), 16 mM (NH4 )2 SO4 , 0.01% Tween 20, 1.5 mM MgCl2 , 50 μM of each deoxynucleoside triphosphate, and 0.5 units of Taq II DNA polymerase (Eurobio). .. To avoid excessive redundant sequencing of FBNSV DNAs, additional PCRs (35 cycles of 95°C for 30 s, 53°C for 30 s, and 72°C for 90 s) using component-specific primers as specified in Table S1 in the supplemental material were run.

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  • 92
    Eurobio deoxynucleoside triphosphate
    Deoxynucleoside Triphosphate, supplied by Eurobio, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphate/product/Eurobio
    Average 92 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphate - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    91
    Eurobio deoxynucleoside triphosphate dntp solution
    Deoxynucleoside Triphosphate Dntp Solution, supplied by Eurobio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphate dntp solution/product/Eurobio
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphate dntp solution - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

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