Structured Review

Eppendorf AG deoxynucleoside triphosphate
Deoxynucleoside Triphosphate, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxynucleoside triphosphate/product/Eppendorf AG
Average 92 stars, based on 11 article reviews
Price from $9.99 to $1999.99
deoxynucleoside triphosphate - by Bioz Stars, 2020-07
92/100 stars

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Polymerase Chain Reaction:

Article Title: Use of Bifidobacterium dentium as an Indicator of the Origin of Fecal Water Pollution
Article Snippet: .. Each PCR mixture (50 μl) was composed of 50 mM KCl, 10 mM Tris-HCl (pH 8.3), 2 mM MgCl2 , 0.1% Triton X-100, each deoxynucleoside triphosphate at a concentration of 200 μM, the Bifidobacterium genus-specific primers lm26 and lm3 ( ) at a concentration of 0.3 μM, 2 μl of bacterial DNA extraction, and 2 U of Taq DNA polymerase (Eppendorf, Hamburg, Germany). .. The PCR was carried out in a Perkin-Elmer thermal cycler (Perkin-Elmer, Norwalk, Conn.).

Article Title: Detection, characterization, and antibiogram of extended-spectrum beta-lactamase Escherichia coli isolated from bovine milk samples in West Bengal, India
Article Snippet: .. Amplification reaction mixture containing 3 µl DNA templates, 50 pmol the primer set [540 bp] (forwardCTX-M -F 5’-CAATGTGCAGCACCAGTAA-3’ and reverseCTX-M -R 5’-CGCGATATCATTGGTGGTG-3’), 1U GoTaq DNA polymerase (Promega, USA), 200 mM deoxynucleoside triphosphate, 10% dimethyl sulfoxide, and 2 mM MgCl2 was prepared in a 25 µl reaction mixture and used in PCR amplification conducted in a thermocycler (Eppendorf, Germany). .. The PCR amplification was done in the following cycle condition with an initial denaturation at 94°C for 10 min, followed by 30 cycles of denaturation at 94°C for 30 s, annealing at 53°C for 30 s, and elongation at 72°C for 60 s with a 10 min final extension period at 72°C.

Article Title: Molecular identification of bacteria in bronchoalveolar lavage fluid from children with cystic fibrosis
Article Snippet: .. Culture microbiology was performed in the clinical microbiology lab at The Children's Hospital by using standard techniques developed for CF ( ). rRNA gene sequences were amplified in 50 μl of PCRs, which included (final concentrations): 1–50 ng of DNA in reaction mixtures, 1× Hotmaster Taq PCR buffer (Eppendorf), 2.5 mM MgCl2 , 100 μM each deoxynucleoside triphosphate, 300 μM each forward (27F) and reverse (907R) primer ( , ), and 1.25 units of Hotmaster Taq (Eppendorf). .. Reaction mixtures were incubated in a PTC-100 Programmable Thermal Cycler (MJ Research) using a touch-down PCR (TD-PCR) protocol with a 2-min initial denaturation step at 94°C followed by 20 cycles at 92°C for 15 s, 65°C annealing for 15 s, and 65°C extension for 1 min.

Article Title: Monitoring of gene knockouts: genome-wide profiling of conditionally essential genes
Article Snippet: .. Linear amplification of single-stranded DNA flanking the gene-inactivation cassette or the site of transposon insertion For the defined library, the primer extension reactions were carried out in 100 μl of 1× HotMaster PCR reaction buffer (Eppendorf, Hamburg, Germany) containing 2.5 mmol/l MgCl2 , 0.4 mmol/l of each deoxynucleoside triphosphate, 40 μg of genomic DNA (the equivalent of 8 × 109 E. coli genomes), 2 pmol of each of outward-facing biotinylated primers (Up-BIO and Dn-BIO in Table , corresponding to upstream and downstream ends of the gene disruption cassette, respectively), and 10 U HotMasterTaq DNA polymerase (Eppendorf). ..

Article Title: NpdR, a Repressor Involved in 2,4,6-Trinitrophenol Degradation in Rhodococcus opacus HL PM-1
Article Snippet: .. Reaction mixtures (total volume, 25 μl) contained 25 pmol of each primer, 1.5 mM MgCl2 , 4% dimethyl sulfoxide (DMSO), 0.2 mM each deoxynucleoside triphosphate, 0.5 to 1 U of Taq polymerase (Eppendorf-Netheler-Hinz GmbH, Hamburg, Germany), PCR buffer, and 10 ng of genomic DNA. .. Reactions performed with Pwo DNA polymerase (Peqlab Biotechnologie GmbH, Erlangen, Germany) were carried out as described above, except that 1 U of enzyme and 2.0 mM Mg2 SO4 were used.

Article Title: Multiplex PCR with 16S rRNA Gene-Targeted Primers of Bifidobacterium spp. To Identify Sources of Fecal Pollution
Article Snippet: .. The ADO-DEN multiplex PCR was performed in 25-μl volumes composed of 50 mM KCl, 10 mM Tris-HCl (pH 8.3), 1.5 mM MgCl2 , 0.1% Triton X-100, each deoxynucleoside triphosphate at a 200 μM concentration, the species-specific primers for B. adolescentis at an 0.25 μM concentration , the species-specific primer for B. dentium at an 0.50 μM concentration, 1 μl of the extracted bacterial DNA, and 0.9 U of Taq DNA polymerase (Eppendorf, Hamburg, Germany). .. The PCR was carried out in a Perkin-Elmer thermal cycler (Perkin-Elmer, Norwalk, Conn.).

Concentration Assay:

Article Title: Use of Bifidobacterium dentium as an Indicator of the Origin of Fecal Water Pollution
Article Snippet: .. Each PCR mixture (50 μl) was composed of 50 mM KCl, 10 mM Tris-HCl (pH 8.3), 2 mM MgCl2 , 0.1% Triton X-100, each deoxynucleoside triphosphate at a concentration of 200 μM, the Bifidobacterium genus-specific primers lm26 and lm3 ( ) at a concentration of 0.3 μM, 2 μl of bacterial DNA extraction, and 2 U of Taq DNA polymerase (Eppendorf, Hamburg, Germany). .. The PCR was carried out in a Perkin-Elmer thermal cycler (Perkin-Elmer, Norwalk, Conn.).

Article Title: Recombination Activity of a Distinctive Integron-Gene Cassette System Associated with Pseudomonas stutzeri Populations in Soil
Article Snippet: .. The amplification mixtures consisted of 10 μl of Genereleaser (Bioventures Inc., Murfreesboro, Tenn.), 50 pmol of each primer, each deoxynucleoside triphosphate at a concentration of 200 nM, 1.5 mM MgCl2 , and 1.5 U of Taq DNA polymerase (Eppendorf) in the buffer supplied with the enzyme. ..

Article Title: Multiplex PCR with 16S rRNA Gene-Targeted Primers of Bifidobacterium spp. To Identify Sources of Fecal Pollution
Article Snippet: .. The ADO-DEN multiplex PCR was performed in 25-μl volumes composed of 50 mM KCl, 10 mM Tris-HCl (pH 8.3), 1.5 mM MgCl2 , 0.1% Triton X-100, each deoxynucleoside triphosphate at a 200 μM concentration, the species-specific primers for B. adolescentis at an 0.25 μM concentration , the species-specific primer for B. dentium at an 0.50 μM concentration, 1 μl of the extracted bacterial DNA, and 0.9 U of Taq DNA polymerase (Eppendorf, Hamburg, Germany). .. The PCR was carried out in a Perkin-Elmer thermal cycler (Perkin-Elmer, Norwalk, Conn.).

Multiplex Assay:

Article Title: Multiplex PCR with 16S rRNA Gene-Targeted Primers of Bifidobacterium spp. To Identify Sources of Fecal Pollution
Article Snippet: .. The ADO-DEN multiplex PCR was performed in 25-μl volumes composed of 50 mM KCl, 10 mM Tris-HCl (pH 8.3), 1.5 mM MgCl2 , 0.1% Triton X-100, each deoxynucleoside triphosphate at a 200 μM concentration, the species-specific primers for B. adolescentis at an 0.25 μM concentration , the species-specific primer for B. dentium at an 0.50 μM concentration, 1 μl of the extracted bacterial DNA, and 0.9 U of Taq DNA polymerase (Eppendorf, Hamburg, Germany). .. The PCR was carried out in a Perkin-Elmer thermal cycler (Perkin-Elmer, Norwalk, Conn.).

Amplification:

Article Title: Detection, characterization, and antibiogram of extended-spectrum beta-lactamase Escherichia coli isolated from bovine milk samples in West Bengal, India
Article Snippet: .. Amplification reaction mixture containing 3 µl DNA templates, 50 pmol the primer set [540 bp] (forwardCTX-M -F 5’-CAATGTGCAGCACCAGTAA-3’ and reverseCTX-M -R 5’-CGCGATATCATTGGTGGTG-3’), 1U GoTaq DNA polymerase (Promega, USA), 200 mM deoxynucleoside triphosphate, 10% dimethyl sulfoxide, and 2 mM MgCl2 was prepared in a 25 µl reaction mixture and used in PCR amplification conducted in a thermocycler (Eppendorf, Germany). .. The PCR amplification was done in the following cycle condition with an initial denaturation at 94°C for 10 min, followed by 30 cycles of denaturation at 94°C for 30 s, annealing at 53°C for 30 s, and elongation at 72°C for 60 s with a 10 min final extension period at 72°C.

Article Title: Molecular identification of bacteria in bronchoalveolar lavage fluid from children with cystic fibrosis
Article Snippet: .. Culture microbiology was performed in the clinical microbiology lab at The Children's Hospital by using standard techniques developed for CF ( ). rRNA gene sequences were amplified in 50 μl of PCRs, which included (final concentrations): 1–50 ng of DNA in reaction mixtures, 1× Hotmaster Taq PCR buffer (Eppendorf), 2.5 mM MgCl2 , 100 μM each deoxynucleoside triphosphate, 300 μM each forward (27F) and reverse (907R) primer ( , ), and 1.25 units of Hotmaster Taq (Eppendorf). .. Reaction mixtures were incubated in a PTC-100 Programmable Thermal Cycler (MJ Research) using a touch-down PCR (TD-PCR) protocol with a 2-min initial denaturation step at 94°C followed by 20 cycles at 92°C for 15 s, 65°C annealing for 15 s, and 65°C extension for 1 min.

Article Title: Monitoring of gene knockouts: genome-wide profiling of conditionally essential genes
Article Snippet: .. Linear amplification of single-stranded DNA flanking the gene-inactivation cassette or the site of transposon insertion For the defined library, the primer extension reactions were carried out in 100 μl of 1× HotMaster PCR reaction buffer (Eppendorf, Hamburg, Germany) containing 2.5 mmol/l MgCl2 , 0.4 mmol/l of each deoxynucleoside triphosphate, 40 μg of genomic DNA (the equivalent of 8 × 109 E. coli genomes), 2 pmol of each of outward-facing biotinylated primers (Up-BIO and Dn-BIO in Table , corresponding to upstream and downstream ends of the gene disruption cassette, respectively), and 10 U HotMasterTaq DNA polymerase (Eppendorf). ..

Article Title: Recombination Activity of a Distinctive Integron-Gene Cassette System Associated with Pseudomonas stutzeri Populations in Soil
Article Snippet: .. The amplification mixtures consisted of 10 μl of Genereleaser (Bioventures Inc., Murfreesboro, Tenn.), 50 pmol of each primer, each deoxynucleoside triphosphate at a concentration of 200 nM, 1.5 mM MgCl2 , and 1.5 U of Taq DNA polymerase (Eppendorf) in the buffer supplied with the enzyme. ..

DNA Extraction:

Article Title: Use of Bifidobacterium dentium as an Indicator of the Origin of Fecal Water Pollution
Article Snippet: .. Each PCR mixture (50 μl) was composed of 50 mM KCl, 10 mM Tris-HCl (pH 8.3), 2 mM MgCl2 , 0.1% Triton X-100, each deoxynucleoside triphosphate at a concentration of 200 μM, the Bifidobacterium genus-specific primers lm26 and lm3 ( ) at a concentration of 0.3 μM, 2 μl of bacterial DNA extraction, and 2 U of Taq DNA polymerase (Eppendorf, Hamburg, Germany). .. The PCR was carried out in a Perkin-Elmer thermal cycler (Perkin-Elmer, Norwalk, Conn.).

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  • 92
    Eppendorf AG deoxynucleotide triphosphate
    Deoxynucleotide Triphosphate, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleotide triphosphate/product/Eppendorf AG
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    deoxynucleotide triphosphate - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    89
    Eppendorf AG deoxynucleotide triphosphate mixture
    Deoxynucleotide Triphosphate Mixture, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleotide triphosphate mixture/product/Eppendorf AG
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deoxynucleotide triphosphate mixture - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

    92
    Eppendorf AG deoxynucleoside triphosphate mixture
    Deoxynucleoside Triphosphate Mixture, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphate mixture/product/Eppendorf AG
    Average 92 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphate mixture - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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