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Boehringer Mannheim deoxynucleoside triphosphate
Deoxynucleoside Triphosphate, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxynucleoside triphosphate/product/Boehringer Mannheim
Average 92 stars, based on 107 article reviews
Price from $9.99 to $1999.99
deoxynucleoside triphosphate - by Bioz Stars, 2020-07
92/100 stars

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Polymerase Chain Reaction:

Article Title: Invasive Streptococcus iniae Infections Outside North America
Article Snippet: .. The PCR mixture (50 μl) contained bacterial DNA, PCR buffer (10 mM Tris-HCl [pH 8.3], 50 mM KCl, 2 mM MgCl2 , 0.01% gelatin), a 200 μM concentration of each deoxynucleoside triphosphate, and 1.0 U of Taq polymerase (Boehringer, Mannheim, Germany). .. The mixtures were amplified in 40 cycles of 94°C for 1 min, 60°C for 1 min and 72°C for 2 min, and a final extension at 72°C for 10 min in an automated thermal cycler (Perkin-Elmer Cetus, Gouda, The Netherlands).

Article Title: Diversity of Endophytic Bacterial Populations and Their Interaction with Xylella fastidiosa in Citrus Plants
Article Snippet: .. The PCR mixture was made up of 2 μl of extracted DNA, 5 μl of 10× Stoffel buffer (10 mM Tris-HCl [pH 8.3], 10 mM KCl), 20 pmol of each primer (Table ), 200 μmol of each deoxynucleoside triphosphate, 3.75 mM MgCl2 , 1% (vol/vol) formamide, 0.25 μg of T4 gene 32 protein (Boehringer Mannheim, Ingelheim, Germany), and 5 U of Taq DNA polymerase Stoffel fragment (Perkin-Elmer Cetus, Nieuwerkerk, The Netherlands) in a 50-μl final volume. ..

Article Title: Molecular Epidemiology of Scedosporium apiospermum Infection Determined by PCR Amplification of Ribosomal Intergenic Spacer Sequences in Patients with Chronic Lung Disease
Article Snippet: .. Each PCR mixture contained 1 U of Taq polymerase (Amplitaq; Perkin-Elmer, Knoxfield, Australia), PCR buffer [67 mM Tris-HCl (pH 8.8), 16.6 mM (NH4)2 SO4 , 0.45% Triton X-100, 0.2 mg of gelatin per ml] (Biotech and Fisher Biotec, Belmont, Australia), 3 mM MgCl2 (Biotech), a 200 μM concentration of each deoxynucleoside triphosphate (Boehringer Mannheim and Roche Diagnostics, Castle View, Australia), a 0.5 μM concentration of each primer, and 4 μl of a 1-in-10 dilution of DNA extract (approximately 80 ng of DNA). .. Each reaction mixture was made up to 20 μl with deionized water treated with diethyl pyrocarbonate (catalog no. D5758; Sigma) and autoclaved.

Article Title: Population Dynamics of a Naturally Occurring Heterogeneous Mixture of Borrelia burgdorferi Clones
Article Snippet: .. Amplifications were performed in a 100-μl reaction mixture containing 1× PCR buffer (Boehringer Mannheim Corp., Indianapolis, Ind.), 10% (vol/vol) glycerol, 1.5 mM MgCl2 , 0.2 mM (each) deoxynucleoside triphosphate, 2.5 U of Taq DNA polymerase (Boehringer Mannheim), and 100 pmol each of primers PC-1 and PC-2. .. For PCR, spirochetes were grown in culture, harvested by centrifugation, and washed 1× in PBS.

Concentration Assay:

Article Title: Invasive Streptococcus iniae Infections Outside North America
Article Snippet: .. The PCR mixture (50 μl) contained bacterial DNA, PCR buffer (10 mM Tris-HCl [pH 8.3], 50 mM KCl, 2 mM MgCl2 , 0.01% gelatin), a 200 μM concentration of each deoxynucleoside triphosphate, and 1.0 U of Taq polymerase (Boehringer, Mannheim, Germany). .. The mixtures were amplified in 40 cycles of 94°C for 1 min, 60°C for 1 min and 72°C for 2 min, and a final extension at 72°C for 10 min in an automated thermal cycler (Perkin-Elmer Cetus, Gouda, The Netherlands).

Article Title: Pre-AIDS Era Isolates of Pneumocystis carinii f. sp. hominis: High Genotypic Similarity with Contemporary Isolates
Article Snippet: .. DNA amplification was carried out on the samples in a reaction mixture containing a final concentration of 10 mM Tris-HCl (pH 8.8), 10 mM KCl, 0.002% Tween 20 (vol/vol), 3 mM MgCl2 , 400 mM (each) deoxynucleoside triphosphate (Boehringer Mannheim), 1 mM (each) oligonucleotide primer, and 0.025 U of Ultma Taq polymerase (Perkin-Elmer Cetus) per μl. .. Nested PCR was used when the amount of P. carinii f. sp. hominis DNA in the samples was low and was insufficient to produce a visible band of amplification product on an ethidium bromide-stained gel after a single round of amplification (Table ).

Article Title: Molecular Epidemiology of Scedosporium apiospermum Infection Determined by PCR Amplification of Ribosomal Intergenic Spacer Sequences in Patients with Chronic Lung Disease
Article Snippet: .. Each PCR mixture contained 1 U of Taq polymerase (Amplitaq; Perkin-Elmer, Knoxfield, Australia), PCR buffer [67 mM Tris-HCl (pH 8.8), 16.6 mM (NH4)2 SO4 , 0.45% Triton X-100, 0.2 mg of gelatin per ml] (Biotech and Fisher Biotec, Belmont, Australia), 3 mM MgCl2 (Biotech), a 200 μM concentration of each deoxynucleoside triphosphate (Boehringer Mannheim and Roche Diagnostics, Castle View, Australia), a 0.5 μM concentration of each primer, and 4 μl of a 1-in-10 dilution of DNA extract (approximately 80 ng of DNA). .. Each reaction mixture was made up to 20 μl with deionized water treated with diethyl pyrocarbonate (catalog no. D5758; Sigma) and autoclaved.

Article Title: Analysis of the Fecal Microflora of Human Subjects Consuming a Probiotic Product Containing Lactobacillus rhamnosus DR20
Article Snippet: .. Each reaction mixture (50 μl) contained reaction buffer (10 mM [final concentration] Tris-HCl, 2.5 mM [final concentration] MgCl2 , 50 mM [final concentration] KCl [pH 8.3]), each deoxynucleoside triphosphate at a concentration of 200 μM, 20 pmol of each primer, 1 μl of fecal DNA, and 2.5 U of Taq DNA polymerase (Boehringer, Mannheim, Germany). .. The following amplification program was used: 94°C for 3 min, 30 cycles consisting of 94°C for 30 s, 56°C for 30 s, and 68°C for 60 s, and then 7 min at 68°C.

Article Title: Usefulness of Multilocus Sequence Typing for Characterization of Clinical Isolates of Candida albicans
Article Snippet: .. PCRs were carried out in 100-μl reaction volumes containing about 0.1 μg of extracted DNA, 100 pmol of each primer, 2.5 U of Taq DNA polymerase (Amersham Pharmacia Biotech, Orsay, France), 10 μl of 10× buffer (supplied with the Taq polymerase), and a 200 μM concentration of each deoxynucleoside triphosphate (Boehringer Mannheim, Meylan, France). ..

Amplification:

Article Title: Pre-AIDS Era Isolates of Pneumocystis carinii f. sp. hominis: High Genotypic Similarity with Contemporary Isolates
Article Snippet: .. DNA amplification was carried out on the samples in a reaction mixture containing a final concentration of 10 mM Tris-HCl (pH 8.8), 10 mM KCl, 0.002% Tween 20 (vol/vol), 3 mM MgCl2 , 400 mM (each) deoxynucleoside triphosphate (Boehringer Mannheim), 1 mM (each) oligonucleotide primer, and 0.025 U of Ultma Taq polymerase (Perkin-Elmer Cetus) per μl. .. Nested PCR was used when the amount of P. carinii f. sp. hominis DNA in the samples was low and was insufficient to produce a visible band of amplification product on an ethidium bromide-stained gel after a single round of amplification (Table ).

Article Title: Proviral Structure, Chromosomal Location, and Expression of HERV-K-T47D, a Novel Human Endogenous Retrovirus Derived from T47D Particles
Article Snippet: .. Amplification of HERV fragments was carried out in a reaction mixture (total volume, 100 μl) containing 10 mM Tris (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , 0.001% gelatin, 0.25 mM each deoxynucleoside triphosphate, 2.5 U of Taq DNA polymerase (Boehringer, Mannheim, Germany), and 50 pmol of each mixed oligonucleotide primer pair. .. Using a Perkin-Elmer Cetus DNA thermal cycler, PCR was performed with the following cycle parameters: a hot start at 94°C for 5 min; 30 cycles of 1 min at 94°C, 2 min at 55 to 65°C, and 2 min at 72°C; and a final extension step of 7 min at 72°C.

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  • 92
    Boehringer Mannheim deoxynucleoside triphosphate
    Deoxynucleoside Triphosphate, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphate/product/Boehringer Mannheim
    Average 92 stars, based on 107 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphate - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    85
    Boehringer Mannheim deoxynucleotide triphosphate labeling mix
    Deoxynucleotide Triphosphate Labeling Mix, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleotide triphosphate labeling mix/product/Boehringer Mannheim
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deoxynucleotide triphosphate labeling mix - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

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