deoxynucleoside triphosphate dntp mixture  (TaKaRa)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    TaKaRa deoxynucleoside triphosphate dntp mixture
    Deoxynucleoside Triphosphate Dntp Mixture, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleoside triphosphate dntp mixture/product/TaKaRa
    Average 90 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    deoxynucleoside triphosphate dntp mixture - by Bioz Stars, 2020-02
    90/100 stars

    Images

    Related Articles

    DNA Extraction:

    Article Title: Up to Species-level Community Analysis of Human Gut Microbiota by 16S rRNA Amplicon Pyrosequencing
    Article Snippet: Feces were collected in individual sterile Faeces Containers (Sarstedt, Nümbrecht, Germany) containing 2 mL RNAlater ® (Ambion, Inc., Austin, TX, USA) and stored at room temperature, taken to the laboratory within 12 hr, and then stored at 4°C until DNA extraction. .. The PCR was performed in a 50 µL volume containing 10 ng to 100 ng extracted DNA as a template, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , 200 µM deoxynucleoside triphosphate (dNTP) mixture, 10 pmol of each primer and 1.25 U TaKaRa Ex Taq HS (Takara Bio, Otsu, Shiga, Japan).

    Multiplex Assay:

    Article Title: Microbial Diversity Analysis of Fermented Mung Beans (Lu-Doh-Huang) by Using Pyrosequencing and Culture Methods
    Article Snippet: Each reaction (volume, 25 µL) contained 10 ng of extracted DNA, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , 200 µM deoxynucleoside triphosphate (dNTP) mixture, 5 pmol of each primer, and 0.625 U ExTaq HS (Takara Bio, Shiga, Japan). .. The underlined sequences are dictated by the requirements of the 454 Sequencing System (Roche Diagnostics, Branford, CT, USA); the nucleotides in lowercase letters are sequence keys used for amplicon sequencing; italicized sequences are those for universal primers for bacteria; and N10 designates the 10-base multiplex identifiers (Roche Diagnostics) used to label each PCR product.

    Amplification:

    Article Title: Diagnostic Limitations to Accurate Diagnosis of Cholera ▿
    Article Snippet: .. Amplification with the three primer pairs for genes (O1 rfb , O139 rfb , and ctxA ) was performed simultaneously in 0.2-ml microcentrifuge tubes, and 3-μl samples were added to the PCR mixture to achieve a 30-μl final volume containing 0.21 mM each deoxynucleoside triphosphate (dNTP) mixture, 50 mM KCl, 1.5 mM MgCl2 , 10 mM Tris-HCl (pH 8.3), 0.17 μM ctxA primer pair, 0.27 μM (each) O1 and O139 rfb primer pairs, and 0.75 units of Taq polymerase (Takara, Kyoto, Japan). .. The conditions for amplification were 5 min at 94°C for initial denaturation of DNA and 35 cycles, each consisting of 1 min at 94°C, 1 min at 55°C, and 1 min at 72°C, with a final round of extension for 7 min at 72°C in a DNA Robo Cycler Gradient Temperature Cycler (Strategene, La Jolla, CA).

    Article Title: Specific Gene Responses of Rhodococcus jostii RHA1 during Growth in Soil
    Article Snippet: PCR was performed in a 25-μl reaction mixture containing 1 μl of a cDNA sample (about 0.4 μg), 25 pmol of specific primers , 2.5 μl of deoxynucleoside triphosphate (dNTP) mixture (2.5 mM each), 2.5 μl of 10× Ex Taq buffer, and 0.5 U of Ex Taq DNA polymerase (TaKaRa Bio Inc.). .. The amplified DNA was subjected to 1.5% agarose gel electrophoresis and visualized with ethidium bromide.

    Article Title: Microbial Diversity Analysis of Fermented Mung Beans (Lu-Doh-Huang) by Using Pyrosequencing and Culture Methods
    Article Snippet: Paragraph title: PCR Amplification for Pyrosequencing ... Each reaction (volume, 25 µL) contained 10 ng of extracted DNA, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , 200 µM deoxynucleoside triphosphate (dNTP) mixture, 5 pmol of each primer, and 0.625 U ExTaq HS (Takara Bio, Shiga, Japan).

    Article Title: Up to Species-level Community Analysis of Human Gut Microbiota by 16S rRNA Amplicon Pyrosequencing
    Article Snippet: The V6-V8 regions of bacterial 16S rRNA genes were amplified by PCR with a bacterial universal primer set, Q-968F-# (5′- CWSWSWWSHT WACGCGARGAACCTTACC-3′) and Q-1390R-# (5′- CWSWSWWSHT TGACGGGC GGTGWGTAC-3′) (# indicates a series of 128 barcode sequence tags underlined in the sequence). .. The PCR was performed in a 50 µL volume containing 10 ng to 100 ng extracted DNA as a template, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , 200 µM deoxynucleoside triphosphate (dNTP) mixture, 10 pmol of each primer and 1.25 U TaKaRa Ex Taq HS (Takara Bio, Otsu, Shiga, Japan).

    Article Title: Direct Assessment of Viral Diversity in Soils by Random PCR Amplification of Polymorphic DNA
    Article Snippet: RAPD-PCR assay mixtures of a 25-μl total volume contained 2.5 μl of 10× Ex Taq buffer (Mg2+ plus), 1.60 μl of a deoxynucleoside triphosphate (dNTP) mixture (0.16 mM each), 2 μl of a 50-pmol μl−1 primer stock (final concentration, 4 μM), 0.50 μl of TaKaRa Ex Taq Hot-Start DNA polymerase (5 units/μl; Hot-Start version; TaKaRa Bio Inc., Otsu, Shiga, Japan), and 1 μl of template (104 , 105 , 106 , or 107 viruses). .. Single primers served as both forward and reverse primers in the PCR amplification.

    Agarose Gel Electrophoresis:

    Article Title: Diagnostic Limitations to Accurate Diagnosis of Cholera ▿
    Article Snippet: Amplification with the three primer pairs for genes (O1 rfb , O139 rfb , and ctxA ) was performed simultaneously in 0.2-ml microcentrifuge tubes, and 3-μl samples were added to the PCR mixture to achieve a 30-μl final volume containing 0.21 mM each deoxynucleoside triphosphate (dNTP) mixture, 50 mM KCl, 1.5 mM MgCl2 , 10 mM Tris-HCl (pH 8.3), 0.17 μM ctxA primer pair, 0.27 μM (each) O1 and O139 rfb primer pairs, and 0.75 units of Taq polymerase (Takara, Kyoto, Japan). .. After amplification, 6 μl of each reaction mixture was subjected to electrophoresis on a 3% agarose gel (11 cm by 14 cm) using a horizontal electrophoresis apparatus (Horizon 11.14; Life Technologies, Gibco BRL).

    Article Title: Specific Gene Responses of Rhodococcus jostii RHA1 during Growth in Soil
    Article Snippet: PCR was performed in a 25-μl reaction mixture containing 1 μl of a cDNA sample (about 0.4 μg), 25 pmol of specific primers , 2.5 μl of deoxynucleoside triphosphate (dNTP) mixture (2.5 mM each), 2.5 μl of 10× Ex Taq buffer, and 0.5 U of Ex Taq DNA polymerase (TaKaRa Bio Inc.). .. The amplified DNA was subjected to 1.5% agarose gel electrophoresis and visualized with ethidium bromide.

    Article Title: Isolation of an Intron-Containing Partial Sequence of the Gene Encoding Dermatophyte Actin (ACT) and Detection of a Fragment of the Transcript by Reverse Transcription-Nested PCR as a Means of Assessing the Viability of Dermatophytes in Skin Scales
    Article Snippet: The PCR product was separated in a 1.5% (wt/vol) low-melting-point agarose gel incorporating ethidium bromide and was purified from the gel using the Geneclean II kit (Bio 101, Inc., Vista, Calif.) according to the manufacturer's instructions. .. The 20-μl transcription reaction mixture contained less than 1 μg of total RNA, 5 mM MgCl2 , 1× RNA PCR buffer, 1 mM deoxynucleoside triphosphate (dNTP) mixture, 1 U of RNase inhibitor per μl, 0.25 U of AMV reverse transcriptase per ml, and 0.125 μM oligo(dT)-adapter primer (Takara Shuzo).

    Synthesized:

    Article Title: Specific Gene Responses of Rhodococcus jostii RHA1 during Growth in Soil
    Article Snippet: The synthesized cDNA was subjected to phenol-chloroform extraction and ethanol precipitation, and it was dissolved in 20 μl of DNase-free water. .. PCR was performed in a 25-μl reaction mixture containing 1 μl of a cDNA sample (about 0.4 μg), 25 pmol of specific primers , 2.5 μl of deoxynucleoside triphosphate (dNTP) mixture (2.5 mM each), 2.5 μl of 10× Ex Taq buffer, and 0.5 U of Ex Taq DNA polymerase (TaKaRa Bio Inc.).

    Ethanol Precipitation:

    Article Title: Specific Gene Responses of Rhodococcus jostii RHA1 during Growth in Soil
    Article Snippet: The synthesized cDNA was subjected to phenol-chloroform extraction and ethanol precipitation, and it was dissolved in 20 μl of DNase-free water. .. PCR was performed in a 25-μl reaction mixture containing 1 μl of a cDNA sample (about 0.4 μg), 25 pmol of specific primers , 2.5 μl of deoxynucleoside triphosphate (dNTP) mixture (2.5 mM each), 2.5 μl of 10× Ex Taq buffer, and 0.5 U of Ex Taq DNA polymerase (TaKaRa Bio Inc.).

    Size-exclusion Chromatography:

    Article Title: Up to Species-level Community Analysis of Human Gut Microbiota by 16S rRNA Amplicon Pyrosequencing
    Article Snippet: The PCR was performed in a 50 µL volume containing 10 ng to 100 ng extracted DNA as a template, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , 200 µM deoxynucleoside triphosphate (dNTP) mixture, 10 pmol of each primer and 1.25 U TaKaRa Ex Taq HS (Takara Bio, Otsu, Shiga, Japan). .. The PCR condition was as follows: 98°C for 2.5 min; 20 cycles at 98°C for 15 sec, 54°C for 30 sec, and 72°C for 20 sec; and finally 72°C for 5 min.

    Quantitative RT-PCR:

    Article Title: Specific Gene Responses of Rhodococcus jostii RHA1 during Growth in Soil
    Article Snippet: Paragraph title: RT-PCR and qRT-PCR. ... PCR was performed in a 25-μl reaction mixture containing 1 μl of a cDNA sample (about 0.4 μg), 25 pmol of specific primers , 2.5 μl of deoxynucleoside triphosphate (dNTP) mixture (2.5 mM each), 2.5 μl of 10× Ex Taq buffer, and 0.5 U of Ex Taq DNA polymerase (TaKaRa Bio Inc.).

    Purification:

    Article Title: Isolation of an Intron-Containing Partial Sequence of the Gene Encoding Dermatophyte Actin (ACT) and Detection of a Fragment of the Transcript by Reverse Transcription-Nested PCR as a Means of Assessing the Viability of Dermatophytes in Skin Scales
    Article Snippet: The PCR product was separated in a 1.5% (wt/vol) low-melting-point agarose gel incorporating ethidium bromide and was purified from the gel using the Geneclean II kit (Bio 101, Inc., Vista, Calif.) according to the manufacturer's instructions. .. The 20-μl transcription reaction mixture contained less than 1 μg of total RNA, 5 mM MgCl2 , 1× RNA PCR buffer, 1 mM deoxynucleoside triphosphate (dNTP) mixture, 1 U of RNase inhibitor per μl, 0.25 U of AMV reverse transcriptase per ml, and 0.125 μM oligo(dT)-adapter primer (Takara Shuzo).

    Article Title: Up to Species-level Community Analysis of Human Gut Microbiota by 16S rRNA Amplicon Pyrosequencing
    Article Snippet: Bacterial DNA was extracted from samples by the bead-beating method and purified as described previously [ ]. .. The PCR was performed in a 50 µL volume containing 10 ng to 100 ng extracted DNA as a template, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , 200 µM deoxynucleoside triphosphate (dNTP) mixture, 10 pmol of each primer and 1.25 U TaKaRa Ex Taq HS (Takara Bio, Otsu, Shiga, Japan).

    Electrophoresis:

    Article Title: Diagnostic Limitations to Accurate Diagnosis of Cholera ▿
    Article Snippet: Amplification with the three primer pairs for genes (O1 rfb , O139 rfb , and ctxA ) was performed simultaneously in 0.2-ml microcentrifuge tubes, and 3-μl samples were added to the PCR mixture to achieve a 30-μl final volume containing 0.21 mM each deoxynucleoside triphosphate (dNTP) mixture, 50 mM KCl, 1.5 mM MgCl2 , 10 mM Tris-HCl (pH 8.3), 0.17 μM ctxA primer pair, 0.27 μM (each) O1 and O139 rfb primer pairs, and 0.75 units of Taq polymerase (Takara, Kyoto, Japan). .. After amplification, 6 μl of each reaction mixture was subjected to electrophoresis on a 3% agarose gel (11 cm by 14 cm) using a horizontal electrophoresis apparatus (Horizon 11.14; Life Technologies, Gibco BRL).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Specific Gene Responses of Rhodococcus jostii RHA1 during Growth in Soil
    Article Snippet: Paragraph title: RT-PCR and qRT-PCR. ... PCR was performed in a 25-μl reaction mixture containing 1 μl of a cDNA sample (about 0.4 μg), 25 pmol of specific primers , 2.5 μl of deoxynucleoside triphosphate (dNTP) mixture (2.5 mM each), 2.5 μl of 10× Ex Taq buffer, and 0.5 U of Ex Taq DNA polymerase (TaKaRa Bio Inc.).

    Article Title: Isolation of an Intron-Containing Partial Sequence of the Gene Encoding Dermatophyte Actin (ACT) and Detection of a Fragment of the Transcript by Reverse Transcription-Nested PCR as a Means of Assessing the Viability of Dermatophytes in Skin Scales
    Article Snippet: Paragraph title: PCR and RT-PCR isolation of the dermatophyte actin gene ( ACT ) fragment. ... The 20-μl transcription reaction mixture contained less than 1 μg of total RNA, 5 mM MgCl2 , 1× RNA PCR buffer, 1 mM deoxynucleoside triphosphate (dNTP) mixture, 1 U of RNase inhibitor per μl, 0.25 U of AMV reverse transcriptase per ml, and 0.125 μM oligo(dT)-adapter primer (Takara Shuzo).

    Incubation:

    Article Title: Isolation of an Intron-Containing Partial Sequence of the Gene Encoding Dermatophyte Actin (ACT) and Detection of a Fragment of the Transcript by Reverse Transcription-Nested PCR as a Means of Assessing the Viability of Dermatophytes in Skin Scales
    Article Snippet: A denaturation step at 94°C for 5 min was followed by 30 cycles of incubation, each consisting of 94°C for 30 s, 55°C for 30 s, 72°C for 30 s, and terminal polymerization at 72°C for 5 min. .. The 20-μl transcription reaction mixture contained less than 1 μg of total RNA, 5 mM MgCl2 , 1× RNA PCR buffer, 1 mM deoxynucleoside triphosphate (dNTP) mixture, 1 U of RNase inhibitor per μl, 0.25 U of AMV reverse transcriptase per ml, and 0.125 μM oligo(dT)-adapter primer (Takara Shuzo).

    Polymerase Chain Reaction:

    Article Title: Diagnostic Limitations to Accurate Diagnosis of Cholera ▿
    Article Snippet: .. Amplification with the three primer pairs for genes (O1 rfb , O139 rfb , and ctxA ) was performed simultaneously in 0.2-ml microcentrifuge tubes, and 3-μl samples were added to the PCR mixture to achieve a 30-μl final volume containing 0.21 mM each deoxynucleoside triphosphate (dNTP) mixture, 50 mM KCl, 1.5 mM MgCl2 , 10 mM Tris-HCl (pH 8.3), 0.17 μM ctxA primer pair, 0.27 μM (each) O1 and O139 rfb primer pairs, and 0.75 units of Taq polymerase (Takara, Kyoto, Japan). .. The conditions for amplification were 5 min at 94°C for initial denaturation of DNA and 35 cycles, each consisting of 1 min at 94°C, 1 min at 55°C, and 1 min at 72°C, with a final round of extension for 7 min at 72°C in a DNA Robo Cycler Gradient Temperature Cycler (Strategene, La Jolla, CA).

    Article Title: Specific Gene Responses of Rhodococcus jostii RHA1 during Growth in Soil
    Article Snippet: .. PCR was performed in a 25-μl reaction mixture containing 1 μl of a cDNA sample (about 0.4 μg), 25 pmol of specific primers , 2.5 μl of deoxynucleoside triphosphate (dNTP) mixture (2.5 mM each), 2.5 μl of 10× Ex Taq buffer, and 0.5 U of Ex Taq DNA polymerase (TaKaRa Bio Inc.). .. The amplified DNA was subjected to 1.5% agarose gel electrophoresis and visualized with ethidium bromide.

    Article Title: Isolation of an Intron-Containing Partial Sequence of the Gene Encoding Dermatophyte Actin (ACT) and Detection of a Fragment of the Transcript by Reverse Transcription-Nested PCR as a Means of Assessing the Viability of Dermatophytes in Skin Scales
    Article Snippet: .. The 20-μl transcription reaction mixture contained less than 1 μg of total RNA, 5 mM MgCl2 , 1× RNA PCR buffer, 1 mM deoxynucleoside triphosphate (dNTP) mixture, 1 U of RNase inhibitor per μl, 0.25 U of AMV reverse transcriptase per ml, and 0.125 μM oligo(dT)-adapter primer (Takara Shuzo). .. The mixture was incubated in the DNA thermal cycler 480 (Perkin-Elmer) at 30°C for 10 min, 50°C for 30 min, 99°C for 5 min, and 4°C for 5 min. PCR was performed in the same tube.

    Article Title: Microbial Diversity Analysis of Fermented Mung Beans (Lu-Doh-Huang) by Using Pyrosequencing and Culture Methods
    Article Snippet: Paragraph title: PCR Amplification for Pyrosequencing ... Each reaction (volume, 25 µL) contained 10 ng of extracted DNA, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , 200 µM deoxynucleoside triphosphate (dNTP) mixture, 5 pmol of each primer, and 0.625 U ExTaq HS (Takara Bio, Shiga, Japan).

    Article Title: Up to Species-level Community Analysis of Human Gut Microbiota by 16S rRNA Amplicon Pyrosequencing
    Article Snippet: .. The PCR was performed in a 50 µL volume containing 10 ng to 100 ng extracted DNA as a template, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , 200 µM deoxynucleoside triphosphate (dNTP) mixture, 10 pmol of each primer and 1.25 U TaKaRa Ex Taq HS (Takara Bio, Otsu, Shiga, Japan). .. The PCR condition was as follows: 98°C for 2.5 min; 20 cycles at 98°C for 15 sec, 54°C for 30 sec, and 72°C for 20 sec; and finally 72°C for 5 min.

    Article Title: Direct Assessment of Viral Diversity in Soils by Random PCR Amplification of Polymorphic DNA
    Article Snippet: RAPD-PCR assay mixtures of a 25-μl total volume contained 2.5 μl of 10× Ex Taq buffer (Mg2+ plus), 1.60 μl of a deoxynucleoside triphosphate (dNTP) mixture (0.16 mM each), 2 μl of a 50-pmol μl−1 primer stock (final concentration, 4 μM), 0.50 μl of TaKaRa Ex Taq Hot-Start DNA polymerase (5 units/μl; Hot-Start version; TaKaRa Bio Inc., Otsu, Shiga, Japan), and 1 μl of template (104 , 105 , 106 , or 107 viruses). .. Single primers served as both forward and reverse primers in the PCR amplification.

    Isolation:

    Article Title: Isolation of an Intron-Containing Partial Sequence of the Gene Encoding Dermatophyte Actin (ACT) and Detection of a Fragment of the Transcript by Reverse Transcription-Nested PCR as a Means of Assessing the Viability of Dermatophytes in Skin Scales
    Article Snippet: Paragraph title: PCR and RT-PCR isolation of the dermatophyte actin gene ( ACT ) fragment. ... The 20-μl transcription reaction mixture contained less than 1 μg of total RNA, 5 mM MgCl2 , 1× RNA PCR buffer, 1 mM deoxynucleoside triphosphate (dNTP) mixture, 1 U of RNase inhibitor per μl, 0.25 U of AMV reverse transcriptase per ml, and 0.125 μM oligo(dT)-adapter primer (Takara Shuzo).

    Sequencing:

    Article Title: Microbial Diversity Analysis of Fermented Mung Beans (Lu-Doh-Huang) by Using Pyrosequencing and Culture Methods
    Article Snippet: Each reaction (volume, 25 µL) contained 10 ng of extracted DNA, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , 200 µM deoxynucleoside triphosphate (dNTP) mixture, 5 pmol of each primer, and 0.625 U ExTaq HS (Takara Bio, Shiga, Japan). .. The underlined sequences are dictated by the requirements of the 454 Sequencing System (Roche Diagnostics, Branford, CT, USA); the nucleotides in lowercase letters are sequence keys used for amplicon sequencing; italicized sequences are those for universal primers for bacteria; and N10 designates the 10-base multiplex identifiers (Roche Diagnostics) used to label each PCR product.

    Article Title: Up to Species-level Community Analysis of Human Gut Microbiota by 16S rRNA Amplicon Pyrosequencing
    Article Snippet: The V6-V8 regions of bacterial 16S rRNA genes were amplified by PCR with a bacterial universal primer set, Q-968F-# (5′- CWSWSWWSHT WACGCGARGAACCTTACC-3′) and Q-1390R-# (5′- CWSWSWWSHT TGACGGGC GGTGWGTAC-3′) (# indicates a series of 128 barcode sequence tags underlined in the sequence). .. The PCR was performed in a 50 µL volume containing 10 ng to 100 ng extracted DNA as a template, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , 200 µM deoxynucleoside triphosphate (dNTP) mixture, 10 pmol of each primer and 1.25 U TaKaRa Ex Taq HS (Takara Bio, Otsu, Shiga, Japan).

    Staining:

    Article Title: Diagnostic Limitations to Accurate Diagnosis of Cholera ▿
    Article Snippet: Amplification with the three primer pairs for genes (O1 rfb , O139 rfb , and ctxA ) was performed simultaneously in 0.2-ml microcentrifuge tubes, and 3-μl samples were added to the PCR mixture to achieve a 30-μl final volume containing 0.21 mM each deoxynucleoside triphosphate (dNTP) mixture, 50 mM KCl, 1.5 mM MgCl2 , 10 mM Tris-HCl (pH 8.3), 0.17 μM ctxA primer pair, 0.27 μM (each) O1 and O139 rfb primer pairs, and 0.75 units of Taq polymerase (Takara, Kyoto, Japan). .. The gel containing amplified DNA was stained with ethidium bromide and visualized using a UV transilluminator.

    Activated Clotting Time Assay:

    Article Title: Isolation of an Intron-Containing Partial Sequence of the Gene Encoding Dermatophyte Actin (ACT) and Detection of a Fragment of the Transcript by Reverse Transcription-Nested PCR as a Means of Assessing the Viability of Dermatophytes in Skin Scales
    Article Snippet: Paragraph title: PCR and RT-PCR isolation of the dermatophyte actin gene ( ACT ) fragment. ... The 20-μl transcription reaction mixture contained less than 1 μg of total RNA, 5 mM MgCl2 , 1× RNA PCR buffer, 1 mM deoxynucleoside triphosphate (dNTP) mixture, 1 U of RNase inhibitor per μl, 0.25 U of AMV reverse transcriptase per ml, and 0.125 μM oligo(dT)-adapter primer (Takara Shuzo).

    Sampling:

    Article Title: Microbial Diversity Analysis of Fermented Mung Beans (Lu-Doh-Huang) by Using Pyrosequencing and Culture Methods
    Article Snippet: PCR Amplification for Pyrosequencing The V1–V2 hypervariable regions of the 16S rRNA genes of bacteria present at each sampling point were amplified individually by using nested PCR as previously described . .. Each reaction (volume, 25 µL) contained 10 ng of extracted DNA, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , 200 µM deoxynucleoside triphosphate (dNTP) mixture, 5 pmol of each primer, and 0.625 U ExTaq HS (Takara Bio, Shiga, Japan).

    Nested PCR:

    Article Title: Microbial Diversity Analysis of Fermented Mung Beans (Lu-Doh-Huang) by Using Pyrosequencing and Culture Methods
    Article Snippet: PCR Amplification for Pyrosequencing The V1–V2 hypervariable regions of the 16S rRNA genes of bacteria present at each sampling point were amplified individually by using nested PCR as previously described . .. Each reaction (volume, 25 µL) contained 10 ng of extracted DNA, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , 200 µM deoxynucleoside triphosphate (dNTP) mixture, 5 pmol of each primer, and 0.625 U ExTaq HS (Takara Bio, Shiga, Japan).

    Concentration Assay:

    Article Title: Direct Assessment of Viral Diversity in Soils by Random PCR Amplification of Polymorphic DNA
    Article Snippet: .. RAPD-PCR assay mixtures of a 25-μl total volume contained 2.5 μl of 10× Ex Taq buffer (Mg2+ plus), 1.60 μl of a deoxynucleoside triphosphate (dNTP) mixture (0.16 mM each), 2 μl of a 50-pmol μl−1 primer stock (final concentration, 4 μM), 0.50 μl of TaKaRa Ex Taq Hot-Start DNA polymerase (5 units/μl; Hot-Start version; TaKaRa Bio Inc., Otsu, Shiga, Japan), and 1 μl of template (104 , 105 , 106 , or 107 viruses). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    TaKaRa deoxynucleotide triphosphate dntp mixture
    Deoxynucleotide Triphosphate Dntp Mixture, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleotide triphosphate dntp mixture/product/TaKaRa
    Average 90 stars, based on 63 article reviews
    Price from $9.99 to $1999.99
    deoxynucleotide triphosphate dntp mixture - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    Image Search Results