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Merck KGaA deoxycholate
Deoxycholate, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deoxycholate/product/Merck KGaA
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
deoxycholate - by Bioz Stars, 2021-04
86/100 stars

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Related Articles

Centrifugation:

Article Title: Signal loss due to oligomerization in ELISA analysis of amyloid-beta can be recovered by a novel sample pre-treatment method
Article Snippet: .. • Resuspend the pellet of the first centrifugation step in 750 μl RIPA-buffer [50 mM Tris-base (Thermo Fisher Scientific), 150 mM NaCl, 0.5% Triton X-100, 1 mM ethylenediaminetetraacetic acid, 3% SDS, 1% deoxycholate, 1 mM PMSF, 2 mM 1,10-phenanthroline monohydrate (Merck Millipore), 1× protease inhibitor cocktail (Sigma–Aldrich), pH 7.40]. .. • Vortex 20 s. • Placed on an overhead shaker (Heidolph) for 15 min at 4 °C.

Protease Inhibitor:

Article Title: Signal loss due to oligomerization in ELISA analysis of amyloid-beta can be recovered by a novel sample pre-treatment method
Article Snippet: .. • Resuspend the pellet of the first centrifugation step in 750 μl RIPA-buffer [50 mM Tris-base (Thermo Fisher Scientific), 150 mM NaCl, 0.5% Triton X-100, 1 mM ethylenediaminetetraacetic acid, 3% SDS, 1% deoxycholate, 1 mM PMSF, 2 mM 1,10-phenanthroline monohydrate (Merck Millipore), 1× protease inhibitor cocktail (Sigma–Aldrich), pH 7.40]. .. • Vortex 20 s. • Placed on an overhead shaker (Heidolph) for 15 min at 4 °C.

Article Title: Systematic functional analysis of kinases in the fungal pathogen Cryptococcus neoformans
Article Snippet: TiO2 enrichment-based phosphoproteomics To identify the phosphorylated targets of Vrk1 on a genome-wide scale, the H99S and vrk1 Δ mutant strains were incubated in YPD liquid medium at 30 °C for 16 h, sub-cultured into 1 l of fresh YPD liquid medium, and further incubated at 30 °C until it approximately reached an optical density at 600 nm (OD600 ) of 0.9. .. Each whole-cell lysate was prepared with lysis buffer containing 50 mM Tris-Cl (pH 7.5), 1% sodium deoxycholate, 5 mM sodium pyrophosphate, 0.2 mM sodium orthovanadate, 50 mM sodium fluoride (NaF), 0.1% sodium dodecyl sulphate, 1% Triton X-100, 0.5 mM phenylmethylsulfonyl fluoride and 2.5 × protease inhibitor cocktail solution (Merck Millipore). .. The protein concentration of each cell lysate was estimated using a Pierce BCA protein kit (Life Technologies).

SDS Page:

Article Title: The Impact of a Nitric Oxide Synthase Inhibitor (L-NAME) on Ischemia–Reperfusion Injury of Cholestatic Livers by Pringle Maneuver and Liver Resection after Bile Duct Ligation in Rats
Article Snippet: NOx, Hyaluronic Acid (HA) and Asymmetric dimethylarginine (ADMA) in Liver Tissue NOx, HA, and ADMA in liver tissue at 1 h and 24 h after reperfusion was measured by the Griess reaction assay using ELISA kits (NO: Cayman Chemical Company, Ann Arbor, MI, USA; HA: TECO medical AG, Sissach, Switzerland; ADMA: Immundiagnostik AG, Bensheim, Germany) according to the manufacturer’s instructions. .. SDS-PAGE and Western Blot Analysis Liver protein extracts were prepared in RIPA buffer containing 20 mM Tris-HCl (pH 7.2), 16 mM NaCl, 2% (w/v) NP-40, 0.1% (w/v) SDS, 0.5% (w/v) sodium deoxycholate and the Complete™-mixture of proteinase inhibitors (Merck KGaA, Darmstadt, Germany). .. Equal amounts of tissue protein extracts (40 µg) were diluted with Nu-PAGE™ LDS electrophoresis sample buffer with DTT as a reducing agent, heated at 95 °C for 10 min, and separated in 4–12% Bis-Tris gradient gels, using MOPS or MES running buffers (all from Invitrogen).

Western Blot:

Article Title: The Impact of a Nitric Oxide Synthase Inhibitor (L-NAME) on Ischemia–Reperfusion Injury of Cholestatic Livers by Pringle Maneuver and Liver Resection after Bile Duct Ligation in Rats
Article Snippet: NOx, Hyaluronic Acid (HA) and Asymmetric dimethylarginine (ADMA) in Liver Tissue NOx, HA, and ADMA in liver tissue at 1 h and 24 h after reperfusion was measured by the Griess reaction assay using ELISA kits (NO: Cayman Chemical Company, Ann Arbor, MI, USA; HA: TECO medical AG, Sissach, Switzerland; ADMA: Immundiagnostik AG, Bensheim, Germany) according to the manufacturer’s instructions. .. SDS-PAGE and Western Blot Analysis Liver protein extracts were prepared in RIPA buffer containing 20 mM Tris-HCl (pH 7.2), 16 mM NaCl, 2% (w/v) NP-40, 0.1% (w/v) SDS, 0.5% (w/v) sodium deoxycholate and the Complete™-mixture of proteinase inhibitors (Merck KGaA, Darmstadt, Germany). .. Equal amounts of tissue protein extracts (40 µg) were diluted with Nu-PAGE™ LDS electrophoresis sample buffer with DTT as a reducing agent, heated at 95 °C for 10 min, and separated in 4–12% Bis-Tris gradient gels, using MOPS or MES running buffers (all from Invitrogen).

Thin Layer Chromatography:

Article Title: Safety Evaluation of Oral Care Probiotics Weissella cibaria CMU and CMS1 by Phenotypic and Genotypic Analysis
Article Snippet: Sep-Paks C18 cartridges (Waters Associates, Milford, MA, USA) were used. .. The secondary bile acids such as deoxycholic acid (DCA) and lithocholic acid (LCA) were detected by TLC plate (Merck, Darmstadt, Germany). ..

Lysis:

Article Title: Systematic functional analysis of kinases in the fungal pathogen Cryptococcus neoformans
Article Snippet: TiO2 enrichment-based phosphoproteomics To identify the phosphorylated targets of Vrk1 on a genome-wide scale, the H99S and vrk1 Δ mutant strains were incubated in YPD liquid medium at 30 °C for 16 h, sub-cultured into 1 l of fresh YPD liquid medium, and further incubated at 30 °C until it approximately reached an optical density at 600 nm (OD600 ) of 0.9. .. Each whole-cell lysate was prepared with lysis buffer containing 50 mM Tris-Cl (pH 7.5), 1% sodium deoxycholate, 5 mM sodium pyrophosphate, 0.2 mM sodium orthovanadate, 50 mM sodium fluoride (NaF), 0.1% sodium dodecyl sulphate, 1% Triton X-100, 0.5 mM phenylmethylsulfonyl fluoride and 2.5 × protease inhibitor cocktail solution (Merck Millipore). .. The protein concentration of each cell lysate was estimated using a Pierce BCA protein kit (Life Technologies).

other:

Article Title: PID15, a novel 6 kDa secreted peptide, mediates Naja naja venom phospholipase A2 induced apoptosis in isolated human peripheral lymphocytes
Article Snippet: Agarose, Bovine Serum Albumin (BSA), trifluoro acetic acid (TFA), acetonitrile, dilinoleoyl phosphatidyl choline (DL-PC), lipoxygenase, deoxycholate, NaCl, KCl, MgSO4 , D-Glucose, EDTA, Diphenyl amine (DPA) were purchased from E-Merck India.

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    Merck KGaA deoxycholate
    Deoxycholate, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxycholate/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deoxycholate - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Merck KGaA sodium deoxycholate
    Sodium Deoxycholate, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sodium deoxycholate/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sodium deoxycholate - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Merck KGaA xylose lysine deoxycholate agar
    Xylose Lysine Deoxycholate Agar, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xylose lysine deoxycholate agar/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xylose lysine deoxycholate agar - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

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