Journal: Osteoarthritis and Cartilage Open

Article Title: PIEZO mechanosensitivity in chondrocytes is differentially modulated by L-type and T-type voltage-sensitive ion channels

doi: 10.1016/j.ocarto.2025.100710

Figure Lengend Snippet: Effect of verapamil on PIEZO1 channel sensitivity. A) Applied deformation after AFM loading. ANOVA testing was significant ( p ​= ​0.0344) but Tukey’s post-hoc testing revealed no significant differences between any groups. B) Magnitude of the intracellular Ca 2+ response to mechanical compression. C) Percentage of cells that responded to AFM compression. D) Magnitude of the intracellular Ca 2+ response to Yoda1 addition. E) Percentage of cells that responded Yoda1 addition. Data presented as mean ​± ​95 ​% confidence interval. One way ANOVA with Tukey’s post-hoc test or Kruskal-Wallis testing with Dunn’s post-hoc test was used for group comparisons A-B and D-E. Different letters indicate statistical significance ( p ​< ​0.05). If the same letter is assigned to multiple groups, those groups are not significantly different from each other. Conversely, if two groups are denoted with completely different letters, the two groups are significantly different. N ​= ​11–48.

Article Snippet: Based on the measured height, the target deformation corresponding to 40, 50, 60, 70, or 80 % of the total thickness was calculated and entered into the Instron software.

Techniques:

Journal: Osteoarthritis and Cartilage Open

Article Title: PIEZO mechanosensitivity in chondrocytes is differentially modulated by L-type and T-type voltage-sensitive ion channels

doi: 10.1016/j.ocarto.2025.100710

Figure Lengend Snippet: Effect of diltiazem hydrochloride on PIEZO1 channel sensitivity. A) Applied deformation after AFM loading. B) Magnitude of the intracellular Ca 2+ response to mechanical compression. C) Percentage of cells that responded to AFM compression. D) Magnitude of the intracellular Ca 2+ response to Yoda1 addition. E) Percentage of cells that responded Yoda1 addition. Data presented as mean ​± ​95 ​% confidence interval. Kruskal-Wallis testing was significant ( p ​= ​0.0343) but Dunn’s test revealed no significant differences between any groups. One way ANOVA with Tukey’s post-hoc test or Kruskal-Wallis testing with Dunn’s post-hoc test was used for group comparisons A-B and D-E. Different letters indicate statistical significance ( p ​< ​0.05). If the same letter is assigned to multiple groups, those groups are not significantly different from each other. Conversely, if two groups are denoted with completely different letters, the two groups are significantly different. N ​= ​14–80.

Article Snippet: Based on the measured height, the target deformation corresponding to 40, 50, 60, 70, or 80 % of the total thickness was calculated and entered into the Instron software.

Techniques:

Journal: Osteoarthritis and Cartilage Open

Article Title: PIEZO mechanosensitivity in chondrocytes is differentially modulated by L-type and T-type voltage-sensitive ion channels

doi: 10.1016/j.ocarto.2025.100710

Figure Lengend Snippet: Effect of nifedipine on PIEZO1 channel sensitivity. A) Applied deformation after AFM loading. B) Magnitude of the intracellular Ca 2+ response to mechanical compression. C) Percentage of cells that responded to AFM compression. D) Magnitude of the intracellular Ca 2+ response to Yoda1 addition. E) Percentage of cells that responded Yoda1 addition. Data presented as mean ​± ​95 ​% confidence interval. One way ANOVA with Tukey’s post-hoc test or Kruskal-Wallis testing with Dunn’s post-hoc test was used for group comparisons A-B and D-E. Different letters indicate statistical significance ( p ​< ​0.05). If the same letter is assigned to multiple groups, those groups are not significantly different from each other. Conversely, if two groups are denoted with completely different letters, the two groups are significantly different. N ​= ​13–40.

Article Snippet: Based on the measured height, the target deformation corresponding to 40, 50, 60, 70, or 80 % of the total thickness was calculated and entered into the Instron software.

Techniques:

Journal: Osteoarthritis and Cartilage Open

Article Title: PIEZO mechanosensitivity in chondrocytes is differentially modulated by L-type and T-type voltage-sensitive ion channels

doi: 10.1016/j.ocarto.2025.100710

Figure Lengend Snippet: Effect of NNC-55 on PIEZO1 channel sensitivity. A) Applied deformation after AFM loading. B) Magnitude of the intracellular Ca 2+ response to mechanical compression. C) Percentage of cells that responded to AFM compression. D) Magnitude of the intracellular Ca 2+ response to Yoda1 addition. E) Percentage of cells that responded Yoda1 addition. Data presented as mean ​± ​95 ​% confidence interval. One way ANOVA with Tukey’s post-hoc test or Kruskal-Wallis testing with Dunn’s post-hoc test was used for group comparisons A-B and D-E. Different letters indicate statistical significance ( p ​< ​0.05). If the same letter is assigned to multiple groups, those groups are not significantly different from each other. Conversely, if two groups are denoted with completely different letters, the two groups are significantly different. N ​= ​10–43.

Article Snippet: Based on the measured height, the target deformation corresponding to 40, 50, 60, 70, or 80 % of the total thickness was calculated and entered into the Instron software.

Techniques:

Journal: Osteoarthritis and Cartilage Open

Article Title: PIEZO mechanosensitivity in chondrocytes is differentially modulated by L-type and T-type voltage-sensitive ion channels

doi: 10.1016/j.ocarto.2025.100710

Figure Lengend Snippet: Live/dead images of the cartilage explants after being deformed to 80 ​% of their height with a loading rate of 0.1 ​mm/s, 0.5 ​mm/s, or 1 ​mm/s revealed extensive surface fractures, indicative of explant destruction. These findings suggested that reducing the deformation magnitude was necessary to preserve explant integrity.

Article Snippet: Based on the measured height, the target deformation corresponding to 40, 50, 60, 70, or 80 % of the total thickness was calculated and entered into the Instron software.

Techniques: