Structured Review

GE Healthcare de81 paper
RT-F155V-H can incorporate rUTP or rGTP into products, using poly(rA)/oligo(dT) or poly(dC)/oligo(dG) as template/primer. Reactions were performed with the indicated template/primer, enzymes, and labeled substrates, and elongated products were assayed by spotting on <t>DE81</t> paper, washing, and autoradiography. Mutant RT-F155V-H was uniquely able to incorporate ribonucleotides.
De81 Paper, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/de81 paper/product/GE Healthcare
Average 90 stars, based on 7 article reviews
Price from $9.99 to $1999.99
de81 paper - by Bioz Stars, 2020-07
90/100 stars

Images

1) Product Images from "Conferring RNA polymerase Activity to a DNA polymerase: A single residue in reverse transcriptase controls substrate selection"

Article Title: Conferring RNA polymerase Activity to a DNA polymerase: A single residue in reverse transcriptase controls substrate selection

Journal: Proceedings of the National Academy of Sciences of the United States of America

doi:

RT-F155V-H can incorporate rUTP or rGTP into products, using poly(rA)/oligo(dT) or poly(dC)/oligo(dG) as template/primer. Reactions were performed with the indicated template/primer, enzymes, and labeled substrates, and elongated products were assayed by spotting on DE81 paper, washing, and autoradiography. Mutant RT-F155V-H was uniquely able to incorporate ribonucleotides.
Figure Legend Snippet: RT-F155V-H can incorporate rUTP or rGTP into products, using poly(rA)/oligo(dT) or poly(dC)/oligo(dG) as template/primer. Reactions were performed with the indicated template/primer, enzymes, and labeled substrates, and elongated products were assayed by spotting on DE81 paper, washing, and autoradiography. Mutant RT-F155V-H was uniquely able to incorporate ribonucleotides.

Techniques Used: Labeling, Autoradiography, Mutagenesis

2) Product Images from "PARP is involved in replicative aging in Neurospora crassa"

Article Title: PARP is involved in replicative aging in Neurospora crassa

Journal: Fungal genetics and biology : FG & B

doi: 10.1016/j.fgb.2009.12.012

Verification of NPO PARylation activity (A) Autoradiogram of a 20% polyacrylamide gel showing 32 P-PAR ladder. PARylation reactions with extracts from wild type N.crassa cells treated (+) or not treated (−) with MMS were run alongside reactions with extracts from npo KO cells treated (+) or not treated (−) with MMS. The lane labeled DW is a negative control reaction using distilled water in place of extract. The right most lane contains a positive control reaction with recombinant human PARP-1 expressed in E. coli . The boxed regions labeled a and b were excised and the radioactivity was eluted for analysis by HPLC.(B)An autoradiogram (BAS2500) of fractions from HPLC blotted onto DE81 paper (Whatman) with retention times indicated above and sample designations on the left. Eluents of 32 P-PAR from these gel slices were either treated with recombinant PARG, or not, and fractionated by HPLC as described in Materials and Methods. The boxed regions show the peak signals eludated at the retention time for ADP-ribose.
Figure Legend Snippet: Verification of NPO PARylation activity (A) Autoradiogram of a 20% polyacrylamide gel showing 32 P-PAR ladder. PARylation reactions with extracts from wild type N.crassa cells treated (+) or not treated (−) with MMS were run alongside reactions with extracts from npo KO cells treated (+) or not treated (−) with MMS. The lane labeled DW is a negative control reaction using distilled water in place of extract. The right most lane contains a positive control reaction with recombinant human PARP-1 expressed in E. coli . The boxed regions labeled a and b were excised and the radioactivity was eluted for analysis by HPLC.(B)An autoradiogram (BAS2500) of fractions from HPLC blotted onto DE81 paper (Whatman) with retention times indicated above and sample designations on the left. Eluents of 32 P-PAR from these gel slices were either treated with recombinant PARG, or not, and fractionated by HPLC as described in Materials and Methods. The boxed regions show the peak signals eludated at the retention time for ADP-ribose.

Techniques Used: Activity Assay, Labeling, Negative Control, Positive Control, Recombinant, Radioactivity, High Performance Liquid Chromatography

Related Articles

Electrophoresis:

Article Title: Saccharomyces cerevisiae Dmc1 and Rad51 Proteins Preferentially Function with Tid1 and Rad54 Proteins, Respectively, to Promote DNA Strand Invasion during Genetic Recombination *
Article Snippet: .. Following electrophoresis, the gels were dried on DE81 paper (Whatman) and exposed to a storage phosphor screen. .. The reaction products were visualized and quantified with a GE Healthcare Storm 860 using ImageQuant version 5.2.

Agarose Gel Electrophoresis:

Article Title: Interaction of T4 UvsW helicase and single-stranded DNA binding protein gp32 through its carboxy terminal acidic tail
Article Snippet: .. Upon completion of the agarose gel electrophoresis, the gel was transferred onto a DE81 paper and vacuum dried on the DE81 (Whatman) paper for 1 h at 60 °C. ..

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