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Millipore ddh2o
Ddh2o, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 40 article reviews
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ddh2o - by Bioz Stars, 2022-09
99/100 stars

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  • 96
    Millipore brdu
    Defective DNA replication underscores the thymidine sensitivity of MDA-MB-468 CRISPR-Cas9 generated mtp53-depleted cells. ( A ) A direct assessment of the S-phase population was determined using flow <t>cytometry</t> by measuring the percentage of parental MDA-MB-468 mtp53 R273H and CRISPR-generated mtp53 variant mtp53-depleted C4 and C11 R273H fs 387, and mtp53 R273HΔ381-388 cells that incorporate <t>BrdU</t> 5 hours post release from a double Aph-Thy block. After the first synchronization at G1/S with aphidicolin, all cell populations were released into the cell cycle for 10 hours (a period sufficient for bulk genome duplication) before initiation of the second block with thymidine. Following release from either the first or second block, cells were pulse-labeled with BrdU for 30 min and then harvested either immediately thereafter in the case of samples released from a single Aph-block, or after 5 hours in the case of double-block samples labeled post-release from the Thy-block. Histograms represent the percentage of cells within each population that incorporated BrdU. ( B ) Assembly of DNA replication factors onto chromosomes of parental MDA-MB-468 mtp53 R273H and CRISPR-generated mtp53 mtp53-depleted R273H fs 387 cells during S-phase was measured using the chromatin fractionation assay. Cytosolic and chromatin fractions were prepared as described in
    Brdu, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 1 article reviews
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    brdu - by Bioz Stars, 2022-09
    96/100 stars
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    92
    Millipore concentrations of mmc
    The effect of DNAH2 knockdown on the viability of <t>MMC-treated</t> U2OS cells. (A) The U2OS cell line was transfected with the siDNAH2 or siFANCA, respectively. The <t>siControl</t> was utilized as the control. The RT-PCR assay was performed to measure the expression level of DNAH2 and FANCA , respectively. GAPDH gene was loaded as an internal reference gene. (B) The U2OS cell line was transfected with the siControl, siDNAH2, or siFANCA, respectively. Under the treatment of different concentrations of MMC (0, 50, 100, 150, 200, and 250 ng/mL), we measured the U2OS cell viability in the 3 groups, respectively. (C) and (D) In the presence (+) or absence (−) of 100 ng/mL MMC, we detected the status of the cell cycle and calculated the percentage of G2/M phase. The statistical difference between the MHC (−) and MHC (+) was analyzed by student t test. DNAH2 , Dynein Axonemal Heavy Chain 2; MMC: mitomycin C; RT-PCR: reverse transcription-polymerase chain reaction.
    Concentrations Of Mmc, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/concentrations of mmc/product/Millipore
    Average 92 stars, based on 1 article reviews
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    concentrations of mmc - by Bioz Stars, 2022-09
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    94
    Millipore drugs administration solifenacin
    <t>Solifenacin</t> increases bladder compliance. (A) Cystometry before (control; CON) and following intra-arterial solifenacin (SF) injections with increasing concentrations (10 −5 −10 −1 mg/kg, bolus). Red, blue, green, orange, pink, and black bars at the bottom of IVP mark the cycles displayed in the pressure-volume analysis. (B) Pressure-volume loops showing increasing concentrations of solifenacin progressively increases the slope of the regression line of the filling phase. (C) Dose-response analysis showing solifenacin step-wisely increases the mean compliance (Cm) with an ED50 at about 1.4 × 10 −4 mg/kg. (D) While vehicle solution (VEH) displays no statistical difference in parameters, injection of solifenacin (1 × 10 −1 mg/kg; SF1X10 −1 ) significantly decreases the peak pressure and voiding frequency but increases voided volume and Cm (* p
    Drugs Administration Solifenacin, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/drugs administration solifenacin/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    drugs administration solifenacin - by Bioz Stars, 2022-09
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    90
    Millipore millipore filtered ddh2o
    <t>Solifenacin</t> increases bladder compliance. (A) Cystometry before (control; CON) and following intra-arterial solifenacin (SF) injections with increasing concentrations (10 −5 −10 −1 mg/kg, bolus). Red, blue, green, orange, pink, and black bars at the bottom of IVP mark the cycles displayed in the pressure-volume analysis. (B) Pressure-volume loops showing increasing concentrations of solifenacin progressively increases the slope of the regression line of the filling phase. (C) Dose-response analysis showing solifenacin step-wisely increases the mean compliance (Cm) with an ED50 at about 1.4 × 10 −4 mg/kg. (D) While vehicle solution (VEH) displays no statistical difference in parameters, injection of solifenacin (1 × 10 −1 mg/kg; SF1X10 −1 ) significantly decreases the peak pressure and voiding frequency but increases voided volume and Cm (* p
    Millipore Filtered Ddh2o, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/millipore filtered ddh2o/product/Millipore
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    millipore filtered ddh2o - by Bioz Stars, 2022-09
    90/100 stars
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    Defective DNA replication underscores the thymidine sensitivity of MDA-MB-468 CRISPR-Cas9 generated mtp53-depleted cells. ( A ) A direct assessment of the S-phase population was determined using flow cytometry by measuring the percentage of parental MDA-MB-468 mtp53 R273H and CRISPR-generated mtp53 variant mtp53-depleted C4 and C11 R273H fs 387, and mtp53 R273HΔ381-388 cells that incorporate BrdU 5 hours post release from a double Aph-Thy block. After the first synchronization at G1/S with aphidicolin, all cell populations were released into the cell cycle for 10 hours (a period sufficient for bulk genome duplication) before initiation of the second block with thymidine. Following release from either the first or second block, cells were pulse-labeled with BrdU for 30 min and then harvested either immediately thereafter in the case of samples released from a single Aph-block, or after 5 hours in the case of double-block samples labeled post-release from the Thy-block. Histograms represent the percentage of cells within each population that incorporated BrdU. ( B ) Assembly of DNA replication factors onto chromosomes of parental MDA-MB-468 mtp53 R273H and CRISPR-generated mtp53 mtp53-depleted R273H fs 387 cells during S-phase was measured using the chromatin fractionation assay. Cytosolic and chromatin fractions were prepared as described in

    Journal: Oncotarget

    Article Title: Frame-shift mediated reduction of gain-of-function p53 R273H and deletion of the R273H C-terminus in breast cancer cells result in replication-stress sensitivity

    doi: 10.18632/oncotarget.27975

    Figure Lengend Snippet: Defective DNA replication underscores the thymidine sensitivity of MDA-MB-468 CRISPR-Cas9 generated mtp53-depleted cells. ( A ) A direct assessment of the S-phase population was determined using flow cytometry by measuring the percentage of parental MDA-MB-468 mtp53 R273H and CRISPR-generated mtp53 variant mtp53-depleted C4 and C11 R273H fs 387, and mtp53 R273HΔ381-388 cells that incorporate BrdU 5 hours post release from a double Aph-Thy block. After the first synchronization at G1/S with aphidicolin, all cell populations were released into the cell cycle for 10 hours (a period sufficient for bulk genome duplication) before initiation of the second block with thymidine. Following release from either the first or second block, cells were pulse-labeled with BrdU for 30 min and then harvested either immediately thereafter in the case of samples released from a single Aph-block, or after 5 hours in the case of double-block samples labeled post-release from the Thy-block. Histograms represent the percentage of cells within each population that incorporated BrdU. ( B ) Assembly of DNA replication factors onto chromosomes of parental MDA-MB-468 mtp53 R273H and CRISPR-generated mtp53 mtp53-depleted R273H fs 387 cells during S-phase was measured using the chromatin fractionation assay. Cytosolic and chromatin fractions were prepared as described in "Materials and Methods" from mtp53 R273H and mtp53-depleted R273H fs 387 cell populations proliferating asynchronously (samples in lanes represent by (–)) or harvested 0, 1.25, 2.5, 5, and 7.5 hours post release from either HU- or Thy- G1/S synchronization, and then analyzed by SDS-PAGE and western blotting for the indicated proteins. The experiment in (A) was performed twice and that in (B) for two biological replicates with Thy-synchronized cells. Chromatin fractionation often resulted in multiple different migration forms of p53 protein. We think that this occurs due to do with both alternatively posttranslational modified isoforms and degradation products of p53 (but have not verified the reasons). The chromatin bound p53 showed less variation than the cytosolic p53.

    Article Snippet: 5-Bromo-2′-deoxyuridine (BrdU) labeling and measurement of BrdU incorporation by flow cytometry Cell cultures were pulse labeled with 50 μM BrdU (Sigma; 50mM stock prepared in ddH2O) for 30 min, and BrdU incorporated into chromosomal DNA was detected using an Alexa Fluor 488-conjugated anti-BrdU antibody by flow cytometry.

    Techniques: Multiple Displacement Amplification, CRISPR, Generated, Flow Cytometry, Variant Assay, Blocking Assay, Labeling, Fractionation, SDS Page, Western Blot, Migration, Modification

    The effect of DNAH2 knockdown on the viability of MMC-treated U2OS cells. (A) The U2OS cell line was transfected with the siDNAH2 or siFANCA, respectively. The siControl was utilized as the control. The RT-PCR assay was performed to measure the expression level of DNAH2 and FANCA , respectively. GAPDH gene was loaded as an internal reference gene. (B) The U2OS cell line was transfected with the siControl, siDNAH2, or siFANCA, respectively. Under the treatment of different concentrations of MMC (0, 50, 100, 150, 200, and 250 ng/mL), we measured the U2OS cell viability in the 3 groups, respectively. (C) and (D) In the presence (+) or absence (−) of 100 ng/mL MMC, we detected the status of the cell cycle and calculated the percentage of G2/M phase. The statistical difference between the MHC (−) and MHC (+) was analyzed by student t test. DNAH2 , Dynein Axonemal Heavy Chain 2; MMC: mitomycin C; RT-PCR: reverse transcription-polymerase chain reaction.

    Journal: Blood Science

    Article Title: DNAH2 facilitates the homologous recombination repair of Fanconi anemia pathway through modulating FANCD2 ubiquitination

    doi: 10.1097/BS9.0000000000000076

    Figure Lengend Snippet: The effect of DNAH2 knockdown on the viability of MMC-treated U2OS cells. (A) The U2OS cell line was transfected with the siDNAH2 or siFANCA, respectively. The siControl was utilized as the control. The RT-PCR assay was performed to measure the expression level of DNAH2 and FANCA , respectively. GAPDH gene was loaded as an internal reference gene. (B) The U2OS cell line was transfected with the siControl, siDNAH2, or siFANCA, respectively. Under the treatment of different concentrations of MMC (0, 50, 100, 150, 200, and 250 ng/mL), we measured the U2OS cell viability in the 3 groups, respectively. (C) and (D) In the presence (+) or absence (−) of 100 ng/mL MMC, we detected the status of the cell cycle and calculated the percentage of G2/M phase. The statistical difference between the MHC (−) and MHC (+) was analyzed by student t test. DNAH2 , Dynein Axonemal Heavy Chain 2; MMC: mitomycin C; RT-PCR: reverse transcription-polymerase chain reaction.

    Article Snippet: 4.4 MMC sensitivity assay U2OS cell line was transfected with the siControl, siDNAH2, or siFANCA, respectively, and then treated by the different concentrations of MMC (0, 50, 100, 150, 200, 250 ng/mL in ddH2O, Sigma) for 24 h. After the adding of CellTiter 96 Aqueous One Solution Reagent (Promega) for 2 h, cellular viability was measured using an UltraMark Microplate Imaging System (Bio Rad, Hercules, CA) following the manufacturer's protocol.

    Techniques: Transfection, Reverse Transcription Polymerase Chain Reaction, Expressing

    DHAH2 knockdown affects ubiquitination and nuclear localization of FANCD2. (A) U2OS cell line was transfected with the siControl, siDNAH2, or siFANCA, respectively. Under the treatment of MMC for 16 h (+) or not (−), a Western blotting assay was performed using the antibody specific for the FANCD2, DNAH2, FANCA, or Tubulin. (B) An immunofluorescence assay was also performed using the antibody specific for the γ-H2AX and FANCD2. (C) The positive cell rate ( > 5 foci) was measured, and the differences among the groups of siControl, siDNAH2, and siFANCA were analyzed using ANOVA followed by the LSD test. ANOVA: analysis of variance; DNAH2 , Dynein Axonemal Heavy Chain 2; LSD: least significant difference; MMC: mitomycin C.

    Journal: Blood Science

    Article Title: DNAH2 facilitates the homologous recombination repair of Fanconi anemia pathway through modulating FANCD2 ubiquitination

    doi: 10.1097/BS9.0000000000000076

    Figure Lengend Snippet: DHAH2 knockdown affects ubiquitination and nuclear localization of FANCD2. (A) U2OS cell line was transfected with the siControl, siDNAH2, or siFANCA, respectively. Under the treatment of MMC for 16 h (+) or not (−), a Western blotting assay was performed using the antibody specific for the FANCD2, DNAH2, FANCA, or Tubulin. (B) An immunofluorescence assay was also performed using the antibody specific for the γ-H2AX and FANCD2. (C) The positive cell rate ( > 5 foci) was measured, and the differences among the groups of siControl, siDNAH2, and siFANCA were analyzed using ANOVA followed by the LSD test. ANOVA: analysis of variance; DNAH2 , Dynein Axonemal Heavy Chain 2; LSD: least significant difference; MMC: mitomycin C.

    Article Snippet: 4.4 MMC sensitivity assay U2OS cell line was transfected with the siControl, siDNAH2, or siFANCA, respectively, and then treated by the different concentrations of MMC (0, 50, 100, 150, 200, 250 ng/mL in ddH2O, Sigma) for 24 h. After the adding of CellTiter 96 Aqueous One Solution Reagent (Promega) for 2 h, cellular viability was measured using an UltraMark Microplate Imaging System (Bio Rad, Hercules, CA) following the manufacturer's protocol.

    Techniques: Transfection, Western Blot, Immunofluorescence

    Solifenacin increases bladder compliance. (A) Cystometry before (control; CON) and following intra-arterial solifenacin (SF) injections with increasing concentrations (10 −5 −10 −1 mg/kg, bolus). Red, blue, green, orange, pink, and black bars at the bottom of IVP mark the cycles displayed in the pressure-volume analysis. (B) Pressure-volume loops showing increasing concentrations of solifenacin progressively increases the slope of the regression line of the filling phase. (C) Dose-response analysis showing solifenacin step-wisely increases the mean compliance (Cm) with an ED50 at about 1.4 × 10 −4 mg/kg. (D) While vehicle solution (VEH) displays no statistical difference in parameters, injection of solifenacin (1 × 10 −1 mg/kg; SF1X10 −1 ) significantly decreases the peak pressure and voiding frequency but increases voided volume and Cm (* p

    Journal: Frontiers in Pharmacology

    Article Title: Solifenacin/Mirabegron Induces an Acute Compliance Increase in the Filling Phase of the Capacity-Reduced Urinary Bladder: A Pressure-Volume Analysis in Rats

    doi: 10.3389/fphar.2021.657959

    Figure Lengend Snippet: Solifenacin increases bladder compliance. (A) Cystometry before (control; CON) and following intra-arterial solifenacin (SF) injections with increasing concentrations (10 −5 −10 −1 mg/kg, bolus). Red, blue, green, orange, pink, and black bars at the bottom of IVP mark the cycles displayed in the pressure-volume analysis. (B) Pressure-volume loops showing increasing concentrations of solifenacin progressively increases the slope of the regression line of the filling phase. (C) Dose-response analysis showing solifenacin step-wisely increases the mean compliance (Cm) with an ED50 at about 1.4 × 10 −4 mg/kg. (D) While vehicle solution (VEH) displays no statistical difference in parameters, injection of solifenacin (1 × 10 −1 mg/kg; SF1X10 −1 ) significantly decreases the peak pressure and voiding frequency but increases voided volume and Cm (* p

    Article Snippet: Drugs Administration Solifenacin (solifenacin succinate; dissolved in ddH2O) and mirabegron (dissolved in 8% alcohol) were injected into animals (1 × 10−5 −1 × 10−1 mg/kg, bolus; both Sigma-Aldrich, Shanghai, China) via an implanted intra-arterial catheter.

    Techniques: Injection

    Solifenacin ameliorates bladder ligation-reduced compliance. (A) Cystometry before (control; CON) and in response to a partial bladder ligation (PBL) followed by intra-arterial injections of solifenacin (SF; 10 -5 and 10 −1 mg/kg, bolus). Red, blue, and green bars at the bottom of IVP mark the cycles displayed in the pressure-volume analysis. (B) Pressure-volume loops showing a partial bladder ligation reduces the slope of the regression line of the filling phase that is marked reversed by solifenacin with a concentration of 10 −1 mg/kg (PBL + SF10 −1 ). (C) A partial bladder ligation statistically increases the peak pressure, post-void pressure, voiding frequency but decreases voided volume and mean compliance (Cm; * p

    Journal: Frontiers in Pharmacology

    Article Title: Solifenacin/Mirabegron Induces an Acute Compliance Increase in the Filling Phase of the Capacity-Reduced Urinary Bladder: A Pressure-Volume Analysis in Rats

    doi: 10.3389/fphar.2021.657959

    Figure Lengend Snippet: Solifenacin ameliorates bladder ligation-reduced compliance. (A) Cystometry before (control; CON) and in response to a partial bladder ligation (PBL) followed by intra-arterial injections of solifenacin (SF; 10 -5 and 10 −1 mg/kg, bolus). Red, blue, and green bars at the bottom of IVP mark the cycles displayed in the pressure-volume analysis. (B) Pressure-volume loops showing a partial bladder ligation reduces the slope of the regression line of the filling phase that is marked reversed by solifenacin with a concentration of 10 −1 mg/kg (PBL + SF10 −1 ). (C) A partial bladder ligation statistically increases the peak pressure, post-void pressure, voiding frequency but decreases voided volume and mean compliance (Cm; * p

    Article Snippet: Drugs Administration Solifenacin (solifenacin succinate; dissolved in ddH2O) and mirabegron (dissolved in 8% alcohol) were injected into animals (1 × 10−5 −1 × 10−1 mg/kg, bolus; both Sigma-Aldrich, Shanghai, China) via an implanted intra-arterial catheter.

    Techniques: Ligation, Concentration Assay