ddh2 o abi 7500 quantitative pcr system  (Thermo Fisher)


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    Thermo Fisher ddh2 o abi 7500 quantitative pcr system
    Ddh2 O Abi 7500 Quantitative Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ddh2 o abi 7500 quantitative pcr system/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ddh2 o abi 7500 quantitative pcr system - by Bioz Stars, 2022-10
    99/100 stars

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    Thermo Fisher propidium iodide pi
    Smac mimetic LCL161 cooperates with vincristine to induce activation of extrinsic and intrinsic apoptosis and caspase-dependent cell death, G2 cell cycle arrest and reduction of migratory potential in neuroblastoma cell lines Neuroblastoma cell lines were treated with the indicated concentrations of vincristine, LCL161, and 50 μmol/L caspase inhibitors. Expression of cIAP-1, XIAP and β-actin was detected by Western Blot analysis 48 h after treatment initiation (A) (representative images of at least three independent Western Blots are shown). The proportions of apoptotic cells (B) (‡; p≤ 0.05 (NC/VCR-treated vs. NC/VCR/LCL161-treated), * ; p≤ 0.05 (NC/VCR/LCL161-treated vs. caspase inhibitor/VCR/LCL161-treated)), and cells with decreased mitochondrial membrane potential (MMP) (C) were determined by flow cytometry 48 hours after treatment initiation. Activation of caspases-8 (D) and -9 (E) was detected by Caspase-Glo assays 24 h following start of treatment. Flow cytometric cell cycle analysis was performed using <t>propidium</t> iodide DNA staining at 24 h time point (F) . Migrated cells 24 h post treatment start were visualized by fluorescence microscopy (G) and quantified using ImageJ and nucleus counter plugin (H) . Values represent the mean ± SD of three independent experiments. (C-H) * p ≤ 0.05; ** p≤ 0.01 (VCR vs VCR/LCL161).
    Propidium Iodide Pi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher ddh2 o abi 7500 quantitative pcr system
    Smac mimetic LCL161 cooperates with vincristine to induce activation of extrinsic and intrinsic apoptosis and caspase-dependent cell death, G2 cell cycle arrest and reduction of migratory potential in neuroblastoma cell lines Neuroblastoma cell lines were treated with the indicated concentrations of vincristine, LCL161, and 50 μmol/L caspase inhibitors. Expression of cIAP-1, XIAP and β-actin was detected by Western Blot analysis 48 h after treatment initiation (A) (representative images of at least three independent Western Blots are shown). The proportions of apoptotic cells (B) (‡; p≤ 0.05 (NC/VCR-treated vs. NC/VCR/LCL161-treated), * ; p≤ 0.05 (NC/VCR/LCL161-treated vs. caspase inhibitor/VCR/LCL161-treated)), and cells with decreased mitochondrial membrane potential (MMP) (C) were determined by flow cytometry 48 hours after treatment initiation. Activation of caspases-8 (D) and -9 (E) was detected by Caspase-Glo assays 24 h following start of treatment. Flow cytometric cell cycle analysis was performed using <t>propidium</t> iodide DNA staining at 24 h time point (F) . Migrated cells 24 h post treatment start were visualized by fluorescence microscopy (G) and quantified using ImageJ and nucleus counter plugin (H) . Values represent the mean ± SD of three independent experiments. (C-H) * p ≤ 0.05; ** p≤ 0.01 (VCR vs VCR/LCL161).
    Ddh2 O Abi 7500 Quantitative Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ddh2 o abi 7500 quantitative pcr system/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ddh2 o abi 7500 quantitative pcr system - by Bioz Stars, 2022-10
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    Thermo Fisher ultrapure double distilled water
    Smac mimetic LCL161 cooperates with vincristine to induce activation of extrinsic and intrinsic apoptosis and caspase-dependent cell death, G2 cell cycle arrest and reduction of migratory potential in neuroblastoma cell lines Neuroblastoma cell lines were treated with the indicated concentrations of vincristine, LCL161, and 50 μmol/L caspase inhibitors. Expression of cIAP-1, XIAP and β-actin was detected by Western Blot analysis 48 h after treatment initiation (A) (representative images of at least three independent Western Blots are shown). The proportions of apoptotic cells (B) (‡; p≤ 0.05 (NC/VCR-treated vs. NC/VCR/LCL161-treated), * ; p≤ 0.05 (NC/VCR/LCL161-treated vs. caspase inhibitor/VCR/LCL161-treated)), and cells with decreased mitochondrial membrane potential (MMP) (C) were determined by flow cytometry 48 hours after treatment initiation. Activation of caspases-8 (D) and -9 (E) was detected by Caspase-Glo assays 24 h following start of treatment. Flow cytometric cell cycle analysis was performed using <t>propidium</t> iodide DNA staining at 24 h time point (F) . Migrated cells 24 h post treatment start were visualized by fluorescence microscopy (G) and quantified using ImageJ and nucleus counter plugin (H) . Values represent the mean ± SD of three independent experiments. (C-H) * p ≤ 0.05; ** p≤ 0.01 (VCR vs VCR/LCL161).
    Ultrapure Double Distilled Water, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher h2 o
    Smac mimetic LCL161 cooperates with vincristine to induce activation of extrinsic and intrinsic apoptosis and caspase-dependent cell death, G2 cell cycle arrest and reduction of migratory potential in neuroblastoma cell lines Neuroblastoma cell lines were treated with the indicated concentrations of vincristine, LCL161, and 50 μmol/L caspase inhibitors. Expression of cIAP-1, XIAP and β-actin was detected by Western Blot analysis 48 h after treatment initiation (A) (representative images of at least three independent Western Blots are shown). The proportions of apoptotic cells (B) (‡; p≤ 0.05 (NC/VCR-treated vs. NC/VCR/LCL161-treated), * ; p≤ 0.05 (NC/VCR/LCL161-treated vs. caspase inhibitor/VCR/LCL161-treated)), and cells with decreased mitochondrial membrane potential (MMP) (C) were determined by flow cytometry 48 hours after treatment initiation. Activation of caspases-8 (D) and -9 (E) was detected by Caspase-Glo assays 24 h following start of treatment. Flow cytometric cell cycle analysis was performed using <t>propidium</t> iodide DNA staining at 24 h time point (F) . Migrated cells 24 h post treatment start were visualized by fluorescence microscopy (G) and quantified using ImageJ and nucleus counter plugin (H) . Values represent the mean ± SD of three independent experiments. (C-H) * p ≤ 0.05; ** p≤ 0.01 (VCR vs VCR/LCL161).
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    Smac mimetic LCL161 cooperates with vincristine to induce activation of extrinsic and intrinsic apoptosis and caspase-dependent cell death, G2 cell cycle arrest and reduction of migratory potential in neuroblastoma cell lines Neuroblastoma cell lines were treated with the indicated concentrations of vincristine, LCL161, and 50 μmol/L caspase inhibitors. Expression of cIAP-1, XIAP and β-actin was detected by Western Blot analysis 48 h after treatment initiation (A) (representative images of at least three independent Western Blots are shown). The proportions of apoptotic cells (B) (‡; p≤ 0.05 (NC/VCR-treated vs. NC/VCR/LCL161-treated), * ; p≤ 0.05 (NC/VCR/LCL161-treated vs. caspase inhibitor/VCR/LCL161-treated)), and cells with decreased mitochondrial membrane potential (MMP) (C) were determined by flow cytometry 48 hours after treatment initiation. Activation of caspases-8 (D) and -9 (E) was detected by Caspase-Glo assays 24 h following start of treatment. Flow cytometric cell cycle analysis was performed using propidium iodide DNA staining at 24 h time point (F) . Migrated cells 24 h post treatment start were visualized by fluorescence microscopy (G) and quantified using ImageJ and nucleus counter plugin (H) . Values represent the mean ± SD of three independent experiments. (C-H) * p ≤ 0.05; ** p≤ 0.01 (VCR vs VCR/LCL161).

    Journal: Oncotarget

    Article Title: Sensitization of neuroblastoma for vincristine-induced apoptosis by Smac mimetic LCL161 is attended by G2 cell cycle arrest but is independent of NFκB, RIP1 and TNF-α

    doi: 10.18632/oncotarget.21193

    Figure Lengend Snippet: Smac mimetic LCL161 cooperates with vincristine to induce activation of extrinsic and intrinsic apoptosis and caspase-dependent cell death, G2 cell cycle arrest and reduction of migratory potential in neuroblastoma cell lines Neuroblastoma cell lines were treated with the indicated concentrations of vincristine, LCL161, and 50 μmol/L caspase inhibitors. Expression of cIAP-1, XIAP and β-actin was detected by Western Blot analysis 48 h after treatment initiation (A) (representative images of at least three independent Western Blots are shown). The proportions of apoptotic cells (B) (‡; p≤ 0.05 (NC/VCR-treated vs. NC/VCR/LCL161-treated), * ; p≤ 0.05 (NC/VCR/LCL161-treated vs. caspase inhibitor/VCR/LCL161-treated)), and cells with decreased mitochondrial membrane potential (MMP) (C) were determined by flow cytometry 48 hours after treatment initiation. Activation of caspases-8 (D) and -9 (E) was detected by Caspase-Glo assays 24 h following start of treatment. Flow cytometric cell cycle analysis was performed using propidium iodide DNA staining at 24 h time point (F) . Migrated cells 24 h post treatment start were visualized by fluorescence microscopy (G) and quantified using ImageJ and nucleus counter plugin (H) . Values represent the mean ± SD of three independent experiments. (C-H) * p ≤ 0.05; ** p≤ 0.01 (VCR vs VCR/LCL161).

    Article Snippet: Apoptosis was detected by Annexin V-FITC (556419; BD Pharmingen) and propidium iodide (PI) (1 mg/ml in ddH2 O; Invitrogen) staining and flow cytometry.

    Techniques: Activation Assay, Expressing, Western Blot, Flow Cytometry, Cytometry, Cell Cycle Assay, Staining, Fluorescence, Microscopy