Structured Review

Thermo Fisher dde i
Agarose gel electrophoresis band patterns after digestion with <t>Dde</t> I endonuclease within the exon 6 and its flanking region sequence of the sheep POU1F1 gene. Lane M: 100 bp DNA marker. Lane 2-7: D1D1 genotype (199 bp, 118 bp, 83 bp and 58 bp). As one
Dde I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 82/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dde i/product/Thermo Fisher
Average 82 stars, based on 3 article reviews
Price from $9.99 to $1999.99
dde i - by Bioz Stars, 2020-01
82/100 stars

Images

1) Product Images from "Polymorphism of sheep POU1F1 gene exon 6 and 3'UTR region and their association with milk production traits"

Article Title: Polymorphism of sheep POU1F1 gene exon 6 and 3'UTR region and their association with milk production traits

Journal:

doi:

Agarose gel electrophoresis band patterns after digestion with Dde I endonuclease within the exon 6 and its flanking region sequence of the sheep POU1F1 gene. Lane M: 100 bp DNA marker. Lane 2-7: D1D1 genotype (199 bp, 118 bp, 83 bp and 58 bp). As one
Figure Legend Snippet: Agarose gel electrophoresis band patterns after digestion with Dde I endonuclease within the exon 6 and its flanking region sequence of the sheep POU1F1 gene. Lane M: 100 bp DNA marker. Lane 2-7: D1D1 genotype (199 bp, 118 bp, 83 bp and 58 bp). As one

Techniques Used: Agarose Gel Electrophoresis, Sequencing, Marker

The structure of sheep POU1F1 gene and SNPs location for exon 6 and 3'UTR and Alu I, Dde I enzyme restriction sites. Black dots (●) represent mutation points
Figure Legend Snippet: The structure of sheep POU1F1 gene and SNPs location for exon 6 and 3'UTR and Alu I, Dde I enzyme restriction sites. Black dots (●) represent mutation points

Techniques Used: Mutagenesis

2) Product Images from "Association Among SNAP-25 Gene DdeI and MnlI Polymorphisms and Hemodynamic Changes During Methylphenidate Use: A Functional Near-Infrared Spectroscopy Study"

Article Title: Association Among SNAP-25 Gene DdeI and MnlI Polymorphisms and Hemodynamic Changes During Methylphenidate Use: A Functional Near-Infrared Spectroscopy Study

Journal:

doi: 10.1177/1087054710374597

Change of left and right HHb with methylphenidate. Effect of SNAP-25 Dde I polymorphism. Y -axis: HHb during Stroop neutral stimuli minus HHb during Stroop incongruent stimuli.
Figure Legend Snippet: Change of left and right HHb with methylphenidate. Effect of SNAP-25 Dde I polymorphism. Y -axis: HHb during Stroop neutral stimuli minus HHb during Stroop incongruent stimuli.

Techniques Used:

Change of left and right HHb with methylphenidate. Effect of SNAP-25 Mnl I and Dde I polymorphisms combined. Y -axis: HHb during Stroop neutral stimuli minus HHb during Stroop incongruent stimuli.
Figure Legend Snippet: Change of left and right HHb with methylphenidate. Effect of SNAP-25 Mnl I and Dde I polymorphisms combined. Y -axis: HHb during Stroop neutral stimuli minus HHb during Stroop incongruent stimuli.

Techniques Used:

Related Articles

Diagnostic Assay:

Article Title: Beta-Globin Gene Haplotypes Among Cameroonians and Review of the Global Distribution: Is There a Case for a Single Sickle Mutation Origin in Africa?
Article Snippet: Paragraph title: Molecular diagnostic testing for sickle cell anemia (SCA; HbSS) ... A thermocycler (Biorad®, USA) was used to amplify a 770 bp segment of the β-globin gene, followed by Dde I (Gibco-BRL®, USA) restriction analysis of the PCR product, as previously reported (Saiki et al., ).

Nucleic Acid Electrophoresis:

Article Title: Association Among SNAP-25 Gene DdeI and MnlI Polymorphisms and Hemodynamic Changes During Methylphenidate Use: A Functional Near-Infrared Spectroscopy Study
Article Snippet: After amplification PCR products were digested by restriction endonuclease 10 U Dde I (Fermentas, Vilnius, Lithuania) or 10 U Mnl I (Fermentas, Vilnius, Lithuania) for 14 h at 37°C. .. A 100-bp marker (50-bp DNA Ladder, Fermentas) was used as a size standard for each gel lane.

Amplification:

Article Title: Polymorphism of sheep POU1F1 gene exon 6 and 3'UTR region and their association with milk production traits
Article Snippet: PCR amplification For this study according to the ovine ( ) sequence of exon 4-6 of POU1F1 gene, the following PCR primers were designed: Forward: 5'- GTATTGCTG CTAAAGACGCC-3' and Reverse: 5'-GAGGGAAAGA TATAGTGAAAGGG-3'. .. Genotyping 20 μl of PCR product was digested separately with 10 U Dde I and 10 U AIu I (Fermentas GmbH, St. Leon-Rot, Germany) at 37°C overnight.

Article Title: Association Among SNAP-25 Gene DdeI and MnlI Polymorphisms and Hemodynamic Changes During Methylphenidate Use: A Functional Near-Infrared Spectroscopy Study
Article Snippet: PCR conditions were 2 min for initial denaturation at 95°C, 35 cycles at 95°C for 45 s for denaturation, 1 min at 58°C for annealing, and 2 min at 72°C for extension, followed by 7 min at 72°C for final extension. .. After amplification PCR products were digested by restriction endonuclease 10 U Dde I (Fermentas, Vilnius, Lithuania) or 10 U Mnl I (Fermentas, Vilnius, Lithuania) for 14 h at 37°C. .. The genotyping of the SNAP-25 gene Dde I or Mnl I polymorphisms was determined by fragment separation at 120 V for 40 to 50 min on a 3.5% Agarose gel containing 0.5 μg/ml ethidium bromide.

Article Title: Optimization of PCR--RFLP Directly from the Skin and Nails in Cases of Dermatophytosis, Targeting the ITS and the 18S Ribosomal DNA Regions
Article Snippet: Both the PCR amplified products were digested separately by using the Hae III (Bangalore Genei Pvt. .. Ltd, Bangalore, India), Mva I and the Dde I (Fermentas Inc. USA) restriction enzymes and they were incubated at 370C for 2 hours.

In Vitro:

Article Title: DNA Packaging Motor Assembly Intermediate of Bacteriophage ?29
Article Snippet: Calibration was done from micrographs of a carbon-grating replica (Fullam, NY). .. 120-base and 106-base pRNA forms were generated by in vitro T7 transcription from pRT72 plasmid linearized with Dde I and Apa LI and (Invitrogen), respectively, as described. .. Alternatively, 120-base pRNA was produced from in vitro transcription of the Bam HI-linearized pH120RNAH plasmid (Atz et al., unpublished results).

Southern Blot:

Article Title: Isolation and Characterization of Polymorphic DNA from Entamoeba histolytica
Article Snippet: Paragraph title: Southern blot analysis. ... Genomic DNA of isolate HM-1:IMSS clone 9 was digested overnight with 10 U each of restriction enzymes Alu I, Dde I, Dra I, Eco RI, and Rsa I (Gibco-BRL or MBI Fermentas), and fragments were separated by electrophoresis using 0.8% agarose gels in 1× TBE buffer and transferred to BiodyneA membranes (Gibco-BRL) using standard methods.

Clone Assay:

Article Title: DNA Packaging Motor Assembly Intermediate of Bacteriophage ?29
Article Snippet: 120-base and 106-base pRNA forms were generated by in vitro T7 transcription from pRT72 plasmid linearized with Dde I and Apa LI and (Invitrogen), respectively, as described. .. The 71-base pRNA expression construct pH71RNAH was generated by two rounds of PCR from the pRNA gene and by adding hammerhead ribozyme sequences to both ends of the template to generate uniform pRNA ends.

Electrophoresis:

Article Title: Polymorphism of sheep POU1F1 gene exon 6 and 3'UTR region and their association with milk production traits
Article Snippet: The PCR products were separated by electrophoresis on 2% agarose gels (Promega, Madison, WI, United States) in parallel with a 100 bp DNA marker. .. Genotyping 20 μl of PCR product was digested separately with 10 U Dde I and 10 U AIu I (Fermentas GmbH, St. Leon-Rot, Germany) at 37°C overnight.

Article Title: Isolation and Characterization of Polymorphic DNA from Entamoeba histolytica
Article Snippet: PCR products from all five axenic isolates of E. histolytica were cloned pGEM-T Easy vector (Promega) and sequenced as described above. .. Genomic DNA of isolate HM-1:IMSS clone 9 was digested overnight with 10 U each of restriction enzymes Alu I, Dde I, Dra I, Eco RI, and Rsa I (Gibco-BRL or MBI Fermentas), and fragments were separated by electrophoresis using 0.8% agarose gels in 1× TBE buffer and transferred to BiodyneA membranes (Gibco-BRL) using standard methods. .. [α-32 P]dCTP-labeled double-stranded DNA probes were prepared by using the Rediprime II random prime labeling system (Amersham Pharmacia Biotech).

Isolation:

Article Title: A Novel, Essential Control for Clonality Analysis with Human Androgen Receptor Gene Polymerase Chain Reaction
Article Snippet: DNA was isolated with the Puregene DNA isolation kit (Gentra Systems, Minneapolis, MN), both for the hematopoietic samples and the paraffin-embedded samples according to the protocols of the manufacturer. .. Digestion of 0.5 μg of DNA was performed for at least 16 hours at 37°C in a reaction mixture of 25 μl, containing 5 U Dde I (Life Technologies, Gaithersburg, MD) and with or without 25 U of concentrated Hpa II (New England Biolabs, Hitchin, UK) in 1× one-phor-all buffer PLUS (Amersham Pharmacia, Uppsala, Sweden).

Autoradiography:

Article Title: Isolation and Characterization of Polymorphic DNA from Entamoeba histolytica
Article Snippet: Genomic DNA of isolate HM-1:IMSS clone 9 was digested overnight with 10 U each of restriction enzymes Alu I, Dde I, Dra I, Eco RI, and Rsa I (Gibco-BRL or MBI Fermentas), and fragments were separated by electrophoresis using 0.8% agarose gels in 1× TBE buffer and transferred to BiodyneA membranes (Gibco-BRL) using standard methods. .. [α-32 P]dCTP-labeled double-stranded DNA probes were prepared by using the Rediprime II random prime labeling system (Amersham Pharmacia Biotech).

Incubation:

Article Title: Optimization of PCR--RFLP Directly from the Skin and Nails in Cases of Dermatophytosis, Targeting the ITS and the 18S Ribosomal DNA Regions
Article Snippet: Both the PCR amplified products were digested separately by using the Hae III (Bangalore Genei Pvt. .. Ltd, Bangalore, India), Mva I and the Dde I (Fermentas Inc. USA) restriction enzymes and they were incubated at 370C for 2 hours. .. The digested products were electrophoresed in a 2 % agarose gel, stained with ethidium bromide and observed under a UV trans-illuminator.

Construct:

Article Title: DNA Packaging Motor Assembly Intermediate of Bacteriophage ?29
Article Snippet: 120-base and 106-base pRNA forms were generated by in vitro T7 transcription from pRT72 plasmid linearized with Dde I and Apa LI and (Invitrogen), respectively, as described. .. Alternatively, 120-base pRNA was produced from in vitro transcription of the Bam HI-linearized pH120RNAH plasmid (Atz et al., unpublished results).

Purification:

Article Title: DNA Packaging Motor Assembly Intermediate of Bacteriophage ?29
Article Snippet: 120-base and 106-base pRNA forms were generated by in vitro T7 transcription from pRT72 plasmid linearized with Dde I and Apa LI and (Invitrogen), respectively, as described. .. The 71-base pRNA expression construct pH71RNAH was generated by two rounds of PCR from the pRNA gene and by adding hammerhead ribozyme sequences to both ends of the template to generate uniform pRNA ends.

DNA Extraction:

Article Title: A Novel, Essential Control for Clonality Analysis with Human Androgen Receptor Gene Polymerase Chain Reaction
Article Snippet: Paragraph title: DNA Isolation, Digestion, and HUMARA PCR ... Digestion of 0.5 μg of DNA was performed for at least 16 hours at 37°C in a reaction mixture of 25 μl, containing 5 U Dde I (Life Technologies, Gaithersburg, MD) and with or without 25 U of concentrated Hpa II (New England Biolabs, Hitchin, UK) in 1× one-phor-all buffer PLUS (Amersham Pharmacia, Uppsala, Sweden).

Marker:

Article Title: Polymorphism of sheep POU1F1 gene exon 6 and 3'UTR region and their association with milk production traits
Article Snippet: The PCR products were separated by electrophoresis on 2% agarose gels (Promega, Madison, WI, United States) in parallel with a 100 bp DNA marker. .. Genotyping 20 μl of PCR product was digested separately with 10 U Dde I and 10 U AIu I (Fermentas GmbH, St. Leon-Rot, Germany) at 37°C overnight.

Article Title: Association Among SNAP-25 Gene DdeI and MnlI Polymorphisms and Hemodynamic Changes During Methylphenidate Use: A Functional Near-Infrared Spectroscopy Study
Article Snippet: After amplification PCR products were digested by restriction endonuclease 10 U Dde I (Fermentas, Vilnius, Lithuania) or 10 U Mnl I (Fermentas, Vilnius, Lithuania) for 14 h at 37°C. .. The genotyping of the SNAP-25 gene Dde I or Mnl I polymorphisms was determined by fragment separation at 120 V for 40 to 50 min on a 3.5% Agarose gel containing 0.5 μg/ml ethidium bromide.

Generated:

Article Title: DNA Packaging Motor Assembly Intermediate of Bacteriophage ?29
Article Snippet: Calibration was done from micrographs of a carbon-grating replica (Fullam, NY). .. 120-base and 106-base pRNA forms were generated by in vitro T7 transcription from pRT72 plasmid linearized with Dde I and Apa LI and (Invitrogen), respectively, as described. .. Alternatively, 120-base pRNA was produced from in vitro transcription of the Bam HI-linearized pH120RNAH plasmid (Atz et al., unpublished results).

Polymerase Chain Reaction:

Article Title: Polymorphism of sheep POU1F1 gene exon 6 and 3'UTR region and their association with milk production traits
Article Snippet: The PCR products were separated by electrophoresis on 2% agarose gels (Promega, Madison, WI, United States) in parallel with a 100 bp DNA marker. .. Genotyping 20 μl of PCR product was digested separately with 10 U Dde I and 10 U AIu I (Fermentas GmbH, St. Leon-Rot, Germany) at 37°C overnight. .. PCR products of digestion were resolved by electrophoresis on a 4% agarose gel stained with ethidium bromide.

Article Title: Association Among SNAP-25 Gene DdeI and MnlI Polymorphisms and Hemodynamic Changes During Methylphenidate Use: A Functional Near-Infrared Spectroscopy Study
Article Snippet: PCR conditions were 2 min for initial denaturation at 95°C, 35 cycles at 95°C for 45 s for denaturation, 1 min at 58°C for annealing, and 2 min at 72°C for extension, followed by 7 min at 72°C for final extension. .. After amplification PCR products were digested by restriction endonuclease 10 U Dde I (Fermentas, Vilnius, Lithuania) or 10 U Mnl I (Fermentas, Vilnius, Lithuania) for 14 h at 37°C. .. The genotyping of the SNAP-25 gene Dde I or Mnl I polymorphisms was determined by fragment separation at 120 V for 40 to 50 min on a 3.5% Agarose gel containing 0.5 μg/ml ethidium bromide.

Article Title: Optimization of PCR--RFLP Directly from the Skin and Nails in Cases of Dermatophytosis, Targeting the ITS and the 18S Ribosomal DNA Regions
Article Snippet: Paragraph title: PCR – RFLP Analysis ... Ltd, Bangalore, India), Mva I and the Dde I (Fermentas Inc. USA) restriction enzymes and they were incubated at 370C for 2 hours.

Article Title: Beta-Globin Gene Haplotypes Among Cameroonians and Review of the Global Distribution: Is There a Case for a Single Sickle Mutation Origin in Africa?
Article Snippet: SCA diagnosis was carried out using 200 ng of DNA. .. A thermocycler (Biorad®, USA) was used to amplify a 770 bp segment of the β-globin gene, followed by Dde I (Gibco-BRL®, USA) restriction analysis of the PCR product, as previously reported (Saiki et al., ). .. Five restriction fragment length polymorphism (RFLP) regions in the β-gene cluster were amplified using published methods designed to analyze the XmnI (5'Gγ), HindIII (Gγ), HindIII (Aγ), HincII (3?

Article Title: DNA Packaging Motor Assembly Intermediate of Bacteriophage ?29
Article Snippet: 120-base and 106-base pRNA forms were generated by in vitro T7 transcription from pRT72 plasmid linearized with Dde I and Apa LI and (Invitrogen), respectively, as described. .. Alternatively, 120-base pRNA was produced from in vitro transcription of the Bam HI-linearized pH120RNAH plasmid (Atz et al., unpublished results).

Article Title: A Novel, Essential Control for Clonality Analysis with Human Androgen Receptor Gene Polymerase Chain Reaction
Article Snippet: Paragraph title: DNA Isolation, Digestion, and HUMARA PCR ... Digestion of 0.5 μg of DNA was performed for at least 16 hours at 37°C in a reaction mixture of 25 μl, containing 5 U Dde I (Life Technologies, Gaithersburg, MD) and with or without 25 U of concentrated Hpa II (New England Biolabs, Hitchin, UK) in 1× one-phor-all buffer PLUS (Amersham Pharmacia, Uppsala, Sweden).

Expressing:

Article Title: DNA Packaging Motor Assembly Intermediate of Bacteriophage ?29
Article Snippet: 120-base and 106-base pRNA forms were generated by in vitro T7 transcription from pRT72 plasmid linearized with Dde I and Apa LI and (Invitrogen), respectively, as described. .. Alternatively, 120-base pRNA was produced from in vitro transcription of the Bam HI-linearized pH120RNAH plasmid (Atz et al., unpublished results).

Sequencing:

Article Title: Polymorphism of sheep POU1F1 gene exon 6 and 3'UTR region and their association with milk production traits
Article Snippet: PCR amplification For this study according to the ovine ( ) sequence of exon 4-6 of POU1F1 gene, the following PCR primers were designed: Forward: 5'- GTATTGCTG CTAAAGACGCC-3' and Reverse: 5'-GAGGGAAAGA TATAGTGAAAGGG-3'. .. Genotyping 20 μl of PCR product was digested separately with 10 U Dde I and 10 U AIu I (Fermentas GmbH, St. Leon-Rot, Germany) at 37°C overnight.

Article Title: Isolation and Characterization of Polymorphic DNA from Entamoeba histolytica
Article Snippet: Genomic DNA of isolate HM-1:IMSS clone 9 was digested overnight with 10 U each of restriction enzymes Alu I, Dde I, Dra I, Eco RI, and Rsa I (Gibco-BRL or MBI Fermentas), and fragments were separated by electrophoresis using 0.8% agarose gels in 1× TBE buffer and transferred to BiodyneA membranes (Gibco-BRL) using standard methods. .. Filters were hybridized overnight at 65°C in a solution of 1 M NaCl–1% sodium dodecyl sulfate–10% dextran sulfate and then washed to a final stringency of 0.2× SSC (1× SSC is 0.15 M NaCl plus 0.015 M sodium citrate)–0.1% sodium dodecyl sulfate at 65°C before autoradiography at −70°C.

Transformation Assay:

Article Title: DNA Packaging Motor Assembly Intermediate of Bacteriophage ?29
Article Snippet: 120-base and 106-base pRNA forms were generated by in vitro T7 transcription from pRT72 plasmid linearized with Dde I and Apa LI and (Invitrogen), respectively, as described. .. The 71-base pRNA expression construct pH71RNAH was generated by two rounds of PCR from the pRNA gene and by adding hammerhead ribozyme sequences to both ends of the template to generate uniform pRNA ends.

Plasmid Preparation:

Article Title: DNA Packaging Motor Assembly Intermediate of Bacteriophage ?29
Article Snippet: Calibration was done from micrographs of a carbon-grating replica (Fullam, NY). .. 120-base and 106-base pRNA forms were generated by in vitro T7 transcription from pRT72 plasmid linearized with Dde I and Apa LI and (Invitrogen), respectively, as described. .. Alternatively, 120-base pRNA was produced from in vitro transcription of the Bam HI-linearized pH120RNAH plasmid (Atz et al., unpublished results).

Software:

Article Title: Polymorphism of sheep POU1F1 gene exon 6 and 3'UTR region and their association with milk production traits
Article Snippet: For primer design, Primer 3 software (Rozen and Skaletsky, 2000 ) was used. .. Genotyping 20 μl of PCR product was digested separately with 10 U Dde I and 10 U AIu I (Fermentas GmbH, St. Leon-Rot, Germany) at 37°C overnight.

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