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Promega dde i
The restriction enzyme analysis on a predicted multiple infection O. tsutsugamushi strain. The DNA pattern on O. tsutsugamushi strain no. 37 undigested PCR product (1) and products after digestion with <t>Dde</t> I enzyme (2). M represents a 100 bp-DNA ladder marker.
Dde I, supplied by Promega, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: High Rates of Homologous Recombination in the Mite Endosymbiont and Opportunistic Human Pathogen Orientia tsutsugamushi

Journal: PLoS Neglected Tropical Diseases

doi: 10.1371/journal.pntd.0000752

The restriction enzyme analysis on a predicted multiple infection O. tsutsugamushi strain. The DNA pattern on O. tsutsugamushi strain no. 37 undigested PCR product (1) and products after digestion with Dde I enzyme (2). M represents a 100 bp-DNA ladder marker.
Figure Legend Snippet: The restriction enzyme analysis on a predicted multiple infection O. tsutsugamushi strain. The DNA pattern on O. tsutsugamushi strain no. 37 undigested PCR product (1) and products after digestion with Dde I enzyme (2). M represents a 100 bp-DNA ladder marker.

Techniques Used: Infection, Polymerase Chain Reaction, Marker

Related Articles

Nucleic Acid Electrophoresis:

Article Title: High Rates of Homologous Recombination in the Mite Endosymbiont and Opportunistic Human Pathogen Orientia tsutsugamushi
Article Snippet: PCR products of locus gpsA from 2 strains (no. 37 and 70) were digested with Dde I (Promega, USA) and Nco I (NEB, England) respectively, as recommended by the manufacturer. .. The restriction enzyme pattern was analysed by gel electrophoresis.

Amplification:

Article Title: Development and Application of a New Scheme for Typing Campylobacter jejuni and Campylobacter coli by PCR-Based Restriction Fragment Length Polymorphism Analysis
Article Snippet: .. After amplification, 10 μl of PCR product was digested with 10 U of restriction enzymes ( Hha I and Nla III [New England Biolabs] and Dde I and Hin dIII [Promega]) in a total volume of 20 μl with 2 μg of bovine serum albumin for more than 3 h at 37°C. .. The digest was analyzed by electrophoresis by using a 1.5% agarose gel and stained with ethidium bromide.

Article Title: A survey of the mycobiota associated with larvae of the black soldier fly (Hermetia illucens) reared for feed production
Article Snippet: .. PCR was performed in 30μl of reaction mixture containing 1X Green GoTaq Reaction Buffer, 0.2mM dNTPs, 0.5μM of each primer, 1.25U GoTaq G2 (Promega Corp., Madison, WI, U.S.A.) and 50ng of template DNA extracted as above, under the following cycling conditions: 94°C for 7 min, 35 cycles at 94°C for 45 sec, 55°C for 45 sec and 72°C for 1 min with a final extension at 72°C for 10 min. Aliquots of 5μl of each amplified product were electrophoretically separated on 1.5% agarose gels in 1X TAE buffer at 90V constant voltage for 30 min. PCR products of the 5.8S-ITS region were digested with the restriction endonucleases Hae III (10U/μl), Dpn I (10U/μl) and Dde I (10U/μl) (Promega Corp., Madison, WI, U.S.A.), in 20μl of reaction volume according to manufacturer instructions and conditions. .. Restriction fragments (ITS-5.8 RFLP’s) were separated on 2% agarose gel in 0.5X TBE buffer for 2 h. Band sizes were estimated by comparison against Bench Top 100bp DNA ladder (Promega Corp., Madison, WI, U.S.A.).

Article Title: Implementing principles of traditional concentrated grape must fermentation to the production of new generation balsamic vinegars. Starter selection and effectiveness
Article Snippet: For the amplification of ITS region of 5.8S rRNA gene, the primer pairs used were ITS1 and ITS4 (White et al. ). .. PCR products were digested without further purification with restriction enzymes Hae III, Cfo I and Dde I (Promega, Milan, Italy).

Agarose Gel Electrophoresis:

Article Title: Development and Application of a New Scheme for Typing Campylobacter jejuni and Campylobacter coli by PCR-Based Restriction Fragment Length Polymorphism Analysis
Article Snippet: After amplification, 10 μl of PCR product was digested with 10 U of restriction enzymes ( Hha I and Nla III [New England Biolabs] and Dde I and Hin dIII [Promega]) in a total volume of 20 μl with 2 μg of bovine serum albumin for more than 3 h at 37°C. .. The digest was analyzed by electrophoresis by using a 1.5% agarose gel and stained with ethidium bromide.

Article Title: Phenotypic and Genotypic Diversity of Thermophilic Campylobacter spp. in Commercial Turkey Flocks: A Longitudinal Study
Article Snippet: .. For fla A-RFLP typing, the PCR product (1.7-kb fragment of the flaA gene) was digested for 5 h at 37°C using Dde I (Promega, USA) (Nachamkin et al. , ) and the digested products were separated using 4% agarose gel in TAE buffer at 50 V for 5 h at room temperature. .. The flaA -RFLP profiles were analyzed using BioNumerics V5 (Applied Maths, Belgium).

Article Title: A survey of the mycobiota associated with larvae of the black soldier fly (Hermetia illucens) reared for feed production
Article Snippet: PCR was performed in 30μl of reaction mixture containing 1X Green GoTaq Reaction Buffer, 0.2mM dNTPs, 0.5μM of each primer, 1.25U GoTaq G2 (Promega Corp., Madison, WI, U.S.A.) and 50ng of template DNA extracted as above, under the following cycling conditions: 94°C for 7 min, 35 cycles at 94°C for 45 sec, 55°C for 45 sec and 72°C for 1 min with a final extension at 72°C for 10 min. Aliquots of 5μl of each amplified product were electrophoretically separated on 1.5% agarose gels in 1X TAE buffer at 90V constant voltage for 30 min. PCR products of the 5.8S-ITS region were digested with the restriction endonucleases Hae III (10U/μl), Dpn I (10U/μl) and Dde I (10U/μl) (Promega Corp., Madison, WI, U.S.A.), in 20μl of reaction volume according to manufacturer instructions and conditions. .. Restriction fragments (ITS-5.8 RFLP’s) were separated on 2% agarose gel in 0.5X TBE buffer for 2 h. Band sizes were estimated by comparison against Bench Top 100bp DNA ladder (Promega Corp., Madison, WI, U.S.A.).

Article Title: Implementing principles of traditional concentrated grape must fermentation to the production of new generation balsamic vinegars. Starter selection and effectiveness
Article Snippet: PCR products were digested without further purification with restriction enzymes Hae III, Cfo I and Dde I (Promega, Milan, Italy). .. Restriction fragments were run onto a 2.0 % (w/v) agarose gel in a 0.5 TBE buffer, stained with ethidium bromide and visualized under UV light.

Plasmid Preparation:

Article Title: High Rates of Homologous Recombination in the Mite Endosymbiont and Opportunistic Human Pathogen Orientia tsutsugamushi
Article Snippet: PCR products of locus gpsA from 2 strains (no. 37 and 70) were digested with Dde I (Promega, USA) and Nco I (NEB, England) respectively, as recommended by the manufacturer. .. For PCR cloning, the PCR products from locus gpsA of strain no. 37 and 70 were blunt-end cloned to pGEM®T easy vector and transformed into E. coli JM109.

Clone Assay:

Article Title: Community terminal restriction fragment length polymorphisms reveal insights into the diversity and dynamics of leaf endophytic bacteria
Article Snippet: To select the endonuclease with the highest power to resolve leaf endophytic bacterial communities, we cloned 16 s rDNA PCR products and randomly selected and sequenced inserts from 50 colonies. .. Therefore, we chose Dde I (Promega) to perform the mono-digestion T-RFLP to generate T-RFLP profiles from five species of plants.

Article Title: High Rates of Homologous Recombination in the Mite Endosymbiont and Opportunistic Human Pathogen Orientia tsutsugamushi
Article Snippet: Paragraph title: Restriction enzyme analysis and cloning ... PCR products of locus gpsA from 2 strains (no. 37 and 70) were digested with Dde I (Promega, USA) and Nco I (NEB, England) respectively, as recommended by the manufacturer.

Lambda DNA Preparation:

Article Title: Development and Application of a New Scheme for Typing Campylobacter jejuni and Campylobacter coli by PCR-Based Restriction Fragment Length Polymorphism Analysis
Article Snippet: After amplification, 10 μl of PCR product was digested with 10 U of restriction enzymes ( Hha I and Nla III [New England Biolabs] and Dde I and Hin dIII [Promega]) in a total volume of 20 μl with 2 μg of bovine serum albumin for more than 3 h at 37°C. .. Lambda DNA digested with Pst I was used as a reference size marker.

Polymerase Chain Reaction:

Article Title: Development and Application of a New Scheme for Typing Campylobacter jejuni and Campylobacter coli by PCR-Based Restriction Fragment Length Polymorphism Analysis
Article Snippet: .. After amplification, 10 μl of PCR product was digested with 10 U of restriction enzymes ( Hha I and Nla III [New England Biolabs] and Dde I and Hin dIII [Promega]) in a total volume of 20 μl with 2 μg of bovine serum albumin for more than 3 h at 37°C. .. The digest was analyzed by electrophoresis by using a 1.5% agarose gel and stained with ethidium bromide.

Article Title: Phenotypic and Genotypic Diversity of Thermophilic Campylobacter spp. in Commercial Turkey Flocks: A Longitudinal Study
Article Snippet: .. For fla A-RFLP typing, the PCR product (1.7-kb fragment of the flaA gene) was digested for 5 h at 37°C using Dde I (Promega, USA) (Nachamkin et al. , ) and the digested products were separated using 4% agarose gel in TAE buffer at 50 V for 5 h at room temperature. .. The flaA -RFLP profiles were analyzed using BioNumerics V5 (Applied Maths, Belgium).

Article Title: Community terminal restriction fragment length polymorphisms reveal insights into the diversity and dynamics of leaf endophytic bacteria
Article Snippet: To select the endonuclease with the highest power to resolve leaf endophytic bacterial communities, we cloned 16 s rDNA PCR products and randomly selected and sequenced inserts from 50 colonies. .. Therefore, we chose Dde I (Promega) to perform the mono-digestion T-RFLP to generate T-RFLP profiles from five species of plants.

Article Title: PCR-RFLP assay as an option for primary HPV test
Article Snippet: Restriction fragment length polymorphism (RFLP) HPV DNA positive samples were submitted to a PCR reaction with PGMY09/11 primers to perform PCR-RFLP for HPV genotyping. .. Reaction was performed according to Nobre et al. , with the enzymes Pst I (Promega, USA), Hae III (Promega, Madison USA), Dde I (Promega), and Rsa I (Promega).

Article Title: PCR-RFLP assay as an option for primary HPV test
Article Snippet: HPV DNA positive samples were submitted to a PCR reaction with PGMY09/11 primers to perform PCR-RFLP for HPV genotyping. .. Reaction was performed according to Nobre et al. , with the enzymes Pst I (Promega, USA), Hae III (Promega, Madison USA), Dde I (Promega), and Rsa I (Promega).

Article Title: High Rates of Homologous Recombination in the Mite Endosymbiont and Opportunistic Human Pathogen Orientia tsutsugamushi
Article Snippet: .. PCR products of locus gpsA from 2 strains (no. 37 and 70) were digested with Dde I (Promega, USA) and Nco I (NEB, England) respectively, as recommended by the manufacturer. .. The restriction enzyme pattern was analysed by gel electrophoresis.

Article Title: A survey of the mycobiota associated with larvae of the black soldier fly (Hermetia illucens) reared for feed production
Article Snippet: .. PCR was performed in 30μl of reaction mixture containing 1X Green GoTaq Reaction Buffer, 0.2mM dNTPs, 0.5μM of each primer, 1.25U GoTaq G2 (Promega Corp., Madison, WI, U.S.A.) and 50ng of template DNA extracted as above, under the following cycling conditions: 94°C for 7 min, 35 cycles at 94°C for 45 sec, 55°C for 45 sec and 72°C for 1 min with a final extension at 72°C for 10 min. Aliquots of 5μl of each amplified product were electrophoretically separated on 1.5% agarose gels in 1X TAE buffer at 90V constant voltage for 30 min. PCR products of the 5.8S-ITS region were digested with the restriction endonucleases Hae III (10U/μl), Dpn I (10U/μl) and Dde I (10U/μl) (Promega Corp., Madison, WI, U.S.A.), in 20μl of reaction volume according to manufacturer instructions and conditions. .. Restriction fragments (ITS-5.8 RFLP’s) were separated on 2% agarose gel in 0.5X TBE buffer for 2 h. Band sizes were estimated by comparison against Bench Top 100bp DNA ladder (Promega Corp., Madison, WI, U.S.A.).

Article Title: Implementing principles of traditional concentrated grape must fermentation to the production of new generation balsamic vinegars. Starter selection and effectiveness
Article Snippet: .. PCR products were digested without further purification with restriction enzymes Hae III, Cfo I and Dde I (Promega, Milan, Italy). .. Restriction fragments were run onto a 2.0 % (w/v) agarose gel in a 0.5 TBE buffer, stained with ethidium bromide and visualized under UV light.

Article Title: Geographic and voltinism differentiation among North American Ostrinia nubilalis (European corn borer) mitochondrial cytochrome c oxidase haplotypes
Article Snippet: .. Single 25 µl digests with Dde I, Hae III, Msp I, or Sau3 AI including 5.0 µl of PCR product, 2.5 µl 10X buffer (Promega), 0.1 mg/µl bovine serum albumin, and 0.5 U of enzyme (Promega) were incubated at 37° C for 8 to 14 hr. .. Reactions were loaded on 1.0 mm by 16 cm 6% polyacrylamide (29:1 acrylamide: bisacrylamide) 0.5X Tris borate EDTA gels, and separated at 140 V for 4 h with a 25-bp step-ladder (Promega) for size comparison.

Size-exclusion Chromatography:

Article Title: A survey of the mycobiota associated with larvae of the black soldier fly (Hermetia illucens) reared for feed production
Article Snippet: .. PCR was performed in 30μl of reaction mixture containing 1X Green GoTaq Reaction Buffer, 0.2mM dNTPs, 0.5μM of each primer, 1.25U GoTaq G2 (Promega Corp., Madison, WI, U.S.A.) and 50ng of template DNA extracted as above, under the following cycling conditions: 94°C for 7 min, 35 cycles at 94°C for 45 sec, 55°C for 45 sec and 72°C for 1 min with a final extension at 72°C for 10 min. Aliquots of 5μl of each amplified product were electrophoretically separated on 1.5% agarose gels in 1X TAE buffer at 90V constant voltage for 30 min. PCR products of the 5.8S-ITS region were digested with the restriction endonucleases Hae III (10U/μl), Dpn I (10U/μl) and Dde I (10U/μl) (Promega Corp., Madison, WI, U.S.A.), in 20μl of reaction volume according to manufacturer instructions and conditions. .. Restriction fragments (ITS-5.8 RFLP’s) were separated on 2% agarose gel in 0.5X TBE buffer for 2 h. Band sizes were estimated by comparison against Bench Top 100bp DNA ladder (Promega Corp., Madison, WI, U.S.A.).

Article Title: Geographic and voltinism differentiation among North American Ostrinia nubilalis (European corn borer) mitochondrial cytochrome c oxidase haplotypes
Article Snippet: Thermocycler conditions were 94° C for 2 min, followed by 40 cycles of 94° C for 30 sec, 52° C for 30 sec, and 72° C for 20 sec. .. Single 25 µl digests with Dde I, Hae III, Msp I, or Sau3 AI including 5.0 µl of PCR product, 2.5 µl 10X buffer (Promega), 0.1 mg/µl bovine serum albumin, and 0.5 U of enzyme (Promega) were incubated at 37° C for 8 to 14 hr.

Terminal Restriction Fragment Length Polymorphism:

Article Title: Community terminal restriction fragment length polymorphisms reveal insights into the diversity and dynamics of leaf endophytic bacteria
Article Snippet: .. Therefore, we chose Dde I (Promega) to perform the mono-digestion T-RFLP to generate T-RFLP profiles from five species of plants. ..

Purification:

Article Title: Implementing principles of traditional concentrated grape must fermentation to the production of new generation balsamic vinegars. Starter selection and effectiveness
Article Snippet: .. PCR products were digested without further purification with restriction enzymes Hae III, Cfo I and Dde I (Promega, Milan, Italy). .. Restriction fragments were run onto a 2.0 % (w/v) agarose gel in a 0.5 TBE buffer, stained with ethidium bromide and visualized under UV light.

Electrophoresis:

Article Title: Development and Application of a New Scheme for Typing Campylobacter jejuni and Campylobacter coli by PCR-Based Restriction Fragment Length Polymorphism Analysis
Article Snippet: After amplification, 10 μl of PCR product was digested with 10 U of restriction enzymes ( Hha I and Nla III [New England Biolabs] and Dde I and Hin dIII [Promega]) in a total volume of 20 μl with 2 μg of bovine serum albumin for more than 3 h at 37°C. .. The digest was analyzed by electrophoresis by using a 1.5% agarose gel and stained with ethidium bromide.

Sequencing:

Article Title: High Rates of Homologous Recombination in the Mite Endosymbiont and Opportunistic Human Pathogen Orientia tsutsugamushi
Article Snippet: Restriction enzyme analysis and cloning To verify that the double nucleotide peaks seen on sequencing were due to multiple gene products from two or more alleles of polymorphic genes present in the patient sample (indicative of mixed infection with multiple strains of O. tsutsugamushi ), restriction enzyme analysis and PCR cloning were performed. .. PCR products of locus gpsA from 2 strains (no. 37 and 70) were digested with Dde I (Promega, USA) and Nco I (NEB, England) respectively, as recommended by the manufacturer.

Generated:

Article Title: Community terminal restriction fragment length polymorphisms reveal insights into the diversity and dynamics of leaf endophytic bacteria
Article Snippet: Computer-simulated virtual digestions indicated that Dde I generated the most distinct T-RFs and thus had the highest resolution. .. Therefore, we chose Dde I (Promega) to perform the mono-digestion T-RFLP to generate T-RFLP profiles from five species of plants.

Selection:

Article Title: Community terminal restriction fragment length polymorphisms reveal insights into the diversity and dynamics of leaf endophytic bacteria
Article Snippet: Paragraph title: The selection of restriction endonuclease and T-RFLP ... Therefore, we chose Dde I (Promega) to perform the mono-digestion T-RFLP to generate T-RFLP profiles from five species of plants.

Infection:

Article Title: High Rates of Homologous Recombination in the Mite Endosymbiont and Opportunistic Human Pathogen Orientia tsutsugamushi
Article Snippet: Restriction enzyme analysis and cloning To verify that the double nucleotide peaks seen on sequencing were due to multiple gene products from two or more alleles of polymorphic genes present in the patient sample (indicative of mixed infection with multiple strains of O. tsutsugamushi ), restriction enzyme analysis and PCR cloning were performed. .. PCR products of locus gpsA from 2 strains (no. 37 and 70) were digested with Dde I (Promega, USA) and Nco I (NEB, England) respectively, as recommended by the manufacturer.

Marker:

Article Title: Development and Application of a New Scheme for Typing Campylobacter jejuni and Campylobacter coli by PCR-Based Restriction Fragment Length Polymorphism Analysis
Article Snippet: After amplification, 10 μl of PCR product was digested with 10 U of restriction enzymes ( Hha I and Nla III [New England Biolabs] and Dde I and Hin dIII [Promega]) in a total volume of 20 μl with 2 μg of bovine serum albumin for more than 3 h at 37°C. .. Lambda DNA digested with Pst I was used as a reference size marker.

Article Title: Implementing principles of traditional concentrated grape must fermentation to the production of new generation balsamic vinegars. Starter selection and effectiveness
Article Snippet: PCR products were digested without further purification with restriction enzymes Hae III, Cfo I and Dde I (Promega, Milan, Italy). .. DNA fragment sizes were determined by comparison with a molecular marker 100 bp ladder (New England Bio-Labs, Beverly, USA).

Staining:

Article Title: Development and Application of a New Scheme for Typing Campylobacter jejuni and Campylobacter coli by PCR-Based Restriction Fragment Length Polymorphism Analysis
Article Snippet: After amplification, 10 μl of PCR product was digested with 10 U of restriction enzymes ( Hha I and Nla III [New England Biolabs] and Dde I and Hin dIII [Promega]) in a total volume of 20 μl with 2 μg of bovine serum albumin for more than 3 h at 37°C. .. The digest was analyzed by electrophoresis by using a 1.5% agarose gel and stained with ethidium bromide.

Article Title: Implementing principles of traditional concentrated grape must fermentation to the production of new generation balsamic vinegars. Starter selection and effectiveness
Article Snippet: PCR products were digested without further purification with restriction enzymes Hae III, Cfo I and Dde I (Promega, Milan, Italy). .. Restriction fragments were run onto a 2.0 % (w/v) agarose gel in a 0.5 TBE buffer, stained with ethidium bromide and visualized under UV light.

Article Title: Geographic and voltinism differentiation among North American Ostrinia nubilalis (European corn borer) mitochondrial cytochrome c oxidase haplotypes
Article Snippet: Single 25 µl digests with Dde I, Hae III, Msp I, or Sau3 AI including 5.0 µl of PCR product, 2.5 µl 10X buffer (Promega), 0.1 mg/µl bovine serum albumin, and 0.5 U of enzyme (Promega) were incubated at 37° C for 8 to 14 hr. .. Gels were stained with ethidium bromide, and digital images were taken on a PC-FOTO/Eclipse documentation system (Fotodyne, www.fotodyne.com ).

Transformation Assay:

Article Title: High Rates of Homologous Recombination in the Mite Endosymbiont and Opportunistic Human Pathogen Orientia tsutsugamushi
Article Snippet: PCR products of locus gpsA from 2 strains (no. 37 and 70) were digested with Dde I (Promega, USA) and Nco I (NEB, England) respectively, as recommended by the manufacturer. .. For PCR cloning, the PCR products from locus gpsA of strain no. 37 and 70 were blunt-end cloned to pGEM®T easy vector and transformed into E. coli JM109.

Recombinant:

Article Title: Community terminal restriction fragment length polymorphisms reveal insights into the diversity and dynamics of leaf endophytic bacteria
Article Snippet: Therefore, we chose Dde I (Promega) to perform the mono-digestion T-RFLP to generate T-RFLP profiles from five species of plants. .. DNA fragments were scanned on an ABI 3730 automated DNA sequencer at Oklahoma State University’s Recombinant DNA/Protein Core Facility.

Incubation:

Article Title: Geographic and voltinism differentiation among North American Ostrinia nubilalis (European corn borer) mitochondrial cytochrome c oxidase haplotypes
Article Snippet: .. Single 25 µl digests with Dde I, Hae III, Msp I, or Sau3 AI including 5.0 µl of PCR product, 2.5 µl 10X buffer (Promega), 0.1 mg/µl bovine serum albumin, and 0.5 U of enzyme (Promega) were incubated at 37° C for 8 to 14 hr. .. Reactions were loaded on 1.0 mm by 16 cm 6% polyacrylamide (29:1 acrylamide: bisacrylamide) 0.5X Tris borate EDTA gels, and separated at 140 V for 4 h with a 25-bp step-ladder (Promega) for size comparison.

Concentration Assay:

Article Title: Development and Application of a New Scheme for Typing Campylobacter jejuni and Campylobacter coli by PCR-Based Restriction Fragment Length Polymorphism Analysis
Article Snippet: PCRs were performed as stated above except that a MgSO4 concentration of 2.0 mM was used with 2.5 U of Pfu DNA polymerase (Promega) instead of Taq and a chain extension of 15 min at 72°C per cycle. .. After amplification, 10 μl of PCR product was digested with 10 U of restriction enzymes ( Hha I and Nla III [New England Biolabs] and Dde I and Hin dIII [Promega]) in a total volume of 20 μl with 2 μg of bovine serum albumin for more than 3 h at 37°C.

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    Promega dde i
    Diagrams representing restriction patterns of 16S rRNA gene digested with <t>Dde</t> I ( a ), Hha I ( b ) or <t>Rsa</t> I ( c ). First column in each diagram corresponds to the banding pattern for the 1 Kb ladder
    Dde I, supplied by Promega, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dde i/product/Promega
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dde i - by Bioz Stars, 2020-02
    87/100 stars
      Buy from Supplier

    Image Search Results


    Diagrams representing restriction patterns of 16S rRNA gene digested with Dde I ( a ), Hha I ( b ) or Rsa I ( c ). First column in each diagram corresponds to the banding pattern for the 1 Kb ladder

    Journal: Microbial ecology

    Article Title: Characterization of the Bacterial Diversity in Indo-West Pacific Loliginid and Sepiolid Squid Light Organs

    doi: 10.1007/s00248-012-0099-6

    Figure Lengend Snippet: Diagrams representing restriction patterns of 16S rRNA gene digested with Dde I ( a ), Hha I ( b ) or Rsa I ( c ). First column in each diagram corresponds to the banding pattern for the 1 Kb ladder

    Article Snippet: RFLP analysis was completed as described by Urakawa et al. [ , ] using three restriction endonucleases: Rsa I (5′GTAC3′), Hha I (5′GCGC3′) and Dde I (5′CTNAG3′; Promega Corporation, Madison, WI).

    Techniques:

    The restriction enzyme analysis on a predicted multiple infection O. tsutsugamushi strain. The DNA pattern on O. tsutsugamushi strain no. 37 undigested PCR product (1) and products after digestion with Dde I enzyme (2). M represents a 100 bp-DNA ladder marker.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: High Rates of Homologous Recombination in the Mite Endosymbiont and Opportunistic Human Pathogen Orientia tsutsugamushi

    doi: 10.1371/journal.pntd.0000752

    Figure Lengend Snippet: The restriction enzyme analysis on a predicted multiple infection O. tsutsugamushi strain. The DNA pattern on O. tsutsugamushi strain no. 37 undigested PCR product (1) and products after digestion with Dde I enzyme (2). M represents a 100 bp-DNA ladder marker.

    Article Snippet: PCR products of locus gpsA from 2 strains (no. 37 and 70) were digested with Dde I (Promega, USA) and Nco I (NEB, England) respectively, as recommended by the manufacturer.

    Techniques: Infection, Polymerase Chain Reaction, Marker