Structured Review

Sanofi damgo
Activation of PKC promotes a selective <t>MOR</t> phosphorylation at T370. (A) HEK239 cells stably expressing MOR were either not exposed (−) or exposed to 1 nM, 10 nM, 100 nM, 1 μM or 10 μM PMA for 30 min. Cells were lysed and immunoblotted with anti-pS363, anti-pT370, anti-pS375, anti-pT376 and anti-pT379 antibodies. Blots were stripped and reprobed with the phosphorylation-independent anti-MOR antibody {UMB-3} to confirm equal loading of the gel. Note that PMA stimulates a dose-dependent phosphorylation of T370. S363 is constitutively phosphorylated. (B) Cells were stably exposed to 10 μM <t>DAMGO</t> or 1 μM PMA for 0, 2, 5, 10, 20 or 30 min, and then immunoblotted with anti-pS375 or anti-pT370 antibodies. Blots were stripped and reprobed with the phosphorylation-independent anti-MOR antibody {UMB-3}. Representative results from one of four independent experiments per condition are shown. The position of molecular mass markers is indicated on the left (in kDa). (C) MOR-and T370A-expressing HEK293 cells were either not exposed (−) or exposed to 10 μM DAMGO or 1 μM PMA for 30 min. Cells were then stained with anti-HA antibody, processed for immunofluorescence and examined by confocal microscopy. Note that PMA induces internalization of wild-type MOR but not of the T370A mutant. Representative images from one of three independent experiments performed in duplicate are shown. Scale bar: 20 μm.
Damgo, supplied by Sanofi, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/damgo/product/Sanofi
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
damgo - by Bioz Stars, 2020-09
92/100 stars

Images

1) Product Images from "Heterologous regulation of agonist-independent μ-opioid receptor phosphorylation by protein kinase C"

Article Title: Heterologous regulation of agonist-independent μ-opioid receptor phosphorylation by protein kinase C

Journal: British Journal of Pharmacology

doi: 10.1111/bph.12546

Activation of PKC promotes a selective MOR phosphorylation at T370. (A) HEK239 cells stably expressing MOR were either not exposed (−) or exposed to 1 nM, 10 nM, 100 nM, 1 μM or 10 μM PMA for 30 min. Cells were lysed and immunoblotted with anti-pS363, anti-pT370, anti-pS375, anti-pT376 and anti-pT379 antibodies. Blots were stripped and reprobed with the phosphorylation-independent anti-MOR antibody {UMB-3} to confirm equal loading of the gel. Note that PMA stimulates a dose-dependent phosphorylation of T370. S363 is constitutively phosphorylated. (B) Cells were stably exposed to 10 μM DAMGO or 1 μM PMA for 0, 2, 5, 10, 20 or 30 min, and then immunoblotted with anti-pS375 or anti-pT370 antibodies. Blots were stripped and reprobed with the phosphorylation-independent anti-MOR antibody {UMB-3}. Representative results from one of four independent experiments per condition are shown. The position of molecular mass markers is indicated on the left (in kDa). (C) MOR-and T370A-expressing HEK293 cells were either not exposed (−) or exposed to 10 μM DAMGO or 1 μM PMA for 30 min. Cells were then stained with anti-HA antibody, processed for immunofluorescence and examined by confocal microscopy. Note that PMA induces internalization of wild-type MOR but not of the T370A mutant. Representative images from one of three independent experiments performed in duplicate are shown. Scale bar: 20 μm.
Figure Legend Snippet: Activation of PKC promotes a selective MOR phosphorylation at T370. (A) HEK239 cells stably expressing MOR were either not exposed (−) or exposed to 1 nM, 10 nM, 100 nM, 1 μM or 10 μM PMA for 30 min. Cells were lysed and immunoblotted with anti-pS363, anti-pT370, anti-pS375, anti-pT376 and anti-pT379 antibodies. Blots were stripped and reprobed with the phosphorylation-independent anti-MOR antibody {UMB-3} to confirm equal loading of the gel. Note that PMA stimulates a dose-dependent phosphorylation of T370. S363 is constitutively phosphorylated. (B) Cells were stably exposed to 10 μM DAMGO or 1 μM PMA for 0, 2, 5, 10, 20 or 30 min, and then immunoblotted with anti-pS375 or anti-pT370 antibodies. Blots were stripped and reprobed with the phosphorylation-independent anti-MOR antibody {UMB-3}. Representative results from one of four independent experiments per condition are shown. The position of molecular mass markers is indicated on the left (in kDa). (C) MOR-and T370A-expressing HEK293 cells were either not exposed (−) or exposed to 10 μM DAMGO or 1 μM PMA for 30 min. Cells were then stained with anti-HA antibody, processed for immunofluorescence and examined by confocal microscopy. Note that PMA induces internalization of wild-type MOR but not of the T370A mutant. Representative images from one of three independent experiments performed in duplicate are shown. Scale bar: 20 μm.

Techniques Used: Activation Assay, Stable Transfection, Expressing, Staining, Immunofluorescence, Confocal Microscopy, Mutagenesis

Related Articles

other:

Article Title: Heterologous regulation of agonist-independent μ-opioid receptor phosphorylation by protein kinase C
Article Snippet: The following MOR agonists were obtained from commercial suppliers: morphine (Merck Pharma, Darmstadt, Germany), DAMGO (Sanofi-Aventis, Frankfurt, Germany) and etonitazene (Novartis, Basel, Switzerland).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Sanofi damgo
    Activation of PKC promotes a selective <t>MOR</t> phosphorylation at T370. (A) HEK239 cells stably expressing MOR were either not exposed (−) or exposed to 1 nM, 10 nM, 100 nM, 1 μM or 10 μM PMA for 30 min. Cells were lysed and immunoblotted with anti-pS363, anti-pT370, anti-pS375, anti-pT376 and anti-pT379 antibodies. Blots were stripped and reprobed with the phosphorylation-independent anti-MOR antibody {UMB-3} to confirm equal loading of the gel. Note that PMA stimulates a dose-dependent phosphorylation of T370. S363 is constitutively phosphorylated. (B) Cells were stably exposed to 10 μM <t>DAMGO</t> or 1 μM PMA for 0, 2, 5, 10, 20 or 30 min, and then immunoblotted with anti-pS375 or anti-pT370 antibodies. Blots were stripped and reprobed with the phosphorylation-independent anti-MOR antibody {UMB-3}. Representative results from one of four independent experiments per condition are shown. The position of molecular mass markers is indicated on the left (in kDa). (C) MOR-and T370A-expressing HEK293 cells were either not exposed (−) or exposed to 10 μM DAMGO or 1 μM PMA for 30 min. Cells were then stained with anti-HA antibody, processed for immunofluorescence and examined by confocal microscopy. Note that PMA induces internalization of wild-type MOR but not of the T370A mutant. Representative images from one of three independent experiments performed in duplicate are shown. Scale bar: 20 μm.
    Damgo, supplied by Sanofi, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/damgo/product/Sanofi
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    damgo - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    Image Search Results


    Activation of PKC promotes a selective MOR phosphorylation at T370. (A) HEK239 cells stably expressing MOR were either not exposed (−) or exposed to 1 nM, 10 nM, 100 nM, 1 μM or 10 μM PMA for 30 min. Cells were lysed and immunoblotted with anti-pS363, anti-pT370, anti-pS375, anti-pT376 and anti-pT379 antibodies. Blots were stripped and reprobed with the phosphorylation-independent anti-MOR antibody {UMB-3} to confirm equal loading of the gel. Note that PMA stimulates a dose-dependent phosphorylation of T370. S363 is constitutively phosphorylated. (B) Cells were stably exposed to 10 μM DAMGO or 1 μM PMA for 0, 2, 5, 10, 20 or 30 min, and then immunoblotted with anti-pS375 or anti-pT370 antibodies. Blots were stripped and reprobed with the phosphorylation-independent anti-MOR antibody {UMB-3}. Representative results from one of four independent experiments per condition are shown. The position of molecular mass markers is indicated on the left (in kDa). (C) MOR-and T370A-expressing HEK293 cells were either not exposed (−) or exposed to 10 μM DAMGO or 1 μM PMA for 30 min. Cells were then stained with anti-HA antibody, processed for immunofluorescence and examined by confocal microscopy. Note that PMA induces internalization of wild-type MOR but not of the T370A mutant. Representative images from one of three independent experiments performed in duplicate are shown. Scale bar: 20 μm.

    Journal: British Journal of Pharmacology

    Article Title: Heterologous regulation of agonist-independent μ-opioid receptor phosphorylation by protein kinase C

    doi: 10.1111/bph.12546

    Figure Lengend Snippet: Activation of PKC promotes a selective MOR phosphorylation at T370. (A) HEK239 cells stably expressing MOR were either not exposed (−) or exposed to 1 nM, 10 nM, 100 nM, 1 μM or 10 μM PMA for 30 min. Cells were lysed and immunoblotted with anti-pS363, anti-pT370, anti-pS375, anti-pT376 and anti-pT379 antibodies. Blots were stripped and reprobed with the phosphorylation-independent anti-MOR antibody {UMB-3} to confirm equal loading of the gel. Note that PMA stimulates a dose-dependent phosphorylation of T370. S363 is constitutively phosphorylated. (B) Cells were stably exposed to 10 μM DAMGO or 1 μM PMA for 0, 2, 5, 10, 20 or 30 min, and then immunoblotted with anti-pS375 or anti-pT370 antibodies. Blots were stripped and reprobed with the phosphorylation-independent anti-MOR antibody {UMB-3}. Representative results from one of four independent experiments per condition are shown. The position of molecular mass markers is indicated on the left (in kDa). (C) MOR-and T370A-expressing HEK293 cells were either not exposed (−) or exposed to 10 μM DAMGO or 1 μM PMA for 30 min. Cells were then stained with anti-HA antibody, processed for immunofluorescence and examined by confocal microscopy. Note that PMA induces internalization of wild-type MOR but not of the T370A mutant. Representative images from one of three independent experiments performed in duplicate are shown. Scale bar: 20 μm.

    Article Snippet: The following MOR agonists were obtained from commercial suppliers: morphine (Merck Pharma, Darmstadt, Germany), DAMGO (Sanofi-Aventis, Frankfurt, Germany) and etonitazene (Novartis, Basel, Switzerland).

    Techniques: Activation Assay, Stable Transfection, Expressing, Staining, Immunofluorescence, Confocal Microscopy, Mutagenesis