da tail step  (New England Biolabs)


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    Structured Review

    New England Biolabs da tail step
    Da Tail Step, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/da tail step/product/New England Biolabs
    Average 87 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    da tail step - by Bioz Stars, 2019-10
    87/100 stars

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    dna repair New England Biolabs

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    Related Articles

    Sequencing:

    Article Title: The Genomic Basis of Color Pattern Polymorphism in the Harlequin Ladybird
    Article Snippet: One of the 25 kb sheared DNA sample was additionally size-selected prior to library preparation using the BluePippin system (Sage Science, Beverly, USA) to remove fragments smaller than 10 kb. .. A DNA Repair (NEBNext FFPE Repair Mix M6630) step as well as an End-repair and dA-tail step (NEBNext End repair / dA-tailing Module E7546) were then processed on 0.34 pmols of the sheared DNA sample, followed by ligation of sequencing adapters. .. Then 0.07 pmols of library were loaded onto an R9.5 flow cell containing at least 800 active pores and run for 48 hr, on a MinION sequencer (ONT) and sequenced by the Minknow ONT software (v1.7.10 or v1.7.14).

    Formalin-fixed Paraffin-Embedded:

    Article Title: The Genomic Basis of Color Pattern Polymorphism in the Harlequin Ladybird
    Article Snippet: One of the 25 kb sheared DNA sample was additionally size-selected prior to library preparation using the BluePippin system (Sage Science, Beverly, USA) to remove fragments smaller than 10 kb. .. A DNA Repair (NEBNext FFPE Repair Mix M6630) step as well as an End-repair and dA-tail step (NEBNext End repair / dA-tailing Module E7546) were then processed on 0.34 pmols of the sheared DNA sample, followed by ligation of sequencing adapters. .. Then 0.07 pmols of library were loaded onto an R9.5 flow cell containing at least 800 active pores and run for 48 hr, on a MinION sequencer (ONT) and sequenced by the Minknow ONT software (v1.7.10 or v1.7.14).

    DNA Ligation:

    Article Title: The Genomic Basis of Color Pattern Polymorphism in the Harlequin Ladybird
    Article Snippet: Four Oxford Nanopore Technologies (ONT) libraries were prepared using the Ligation Sequencing Kit 1D (SQK-LSK108 ONT), according to the manufacturer’s protocol 1D Genomic DNA by ligation (SQK-LSK108). .. A DNA Repair (NEBNext FFPE Repair Mix M6630) step as well as an End-repair and dA-tail step (NEBNext End repair / dA-tailing Module E7546) were then processed on 0.34 pmols of the sheared DNA sample, followed by ligation of sequencing adapters.

    Ligation:

    Article Title: The Genomic Basis of Color Pattern Polymorphism in the Harlequin Ladybird
    Article Snippet: One of the 25 kb sheared DNA sample was additionally size-selected prior to library preparation using the BluePippin system (Sage Science, Beverly, USA) to remove fragments smaller than 10 kb. .. A DNA Repair (NEBNext FFPE Repair Mix M6630) step as well as an End-repair and dA-tail step (NEBNext End repair / dA-tailing Module E7546) were then processed on 0.34 pmols of the sheared DNA sample, followed by ligation of sequencing adapters. .. Then 0.07 pmols of library were loaded onto an R9.5 flow cell containing at least 800 active pores and run for 48 hr, on a MinION sequencer (ONT) and sequenced by the Minknow ONT software (v1.7.10 or v1.7.14).

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  • Team
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  • 87
    New England Biolabs da tail step
    Da Tail Step, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/da tail step/product/New England Biolabs
    Average 87 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    da tail step - by Bioz Stars, 2019-10
    87/100 stars
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