cdna library solution  (New England Biolabs)


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    New England Biolabs cdna library solution
    Cdna Library Solution, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cdna library solution/product/New England Biolabs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cdna library solution - by Bioz Stars, 2020-02
    92/100 stars

    Related Products / Commonly Used Together

    dna lo-bind tube
    da-tailing buffer

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    Related Articles

    Agarose Gel Electrophoresis:

    Article Title: Transcriptome Analysis of Gelatin Seed Treatment as a Biostimulant of Cucumber Plant Growth
    Article Snippet: Another round of size selection was performed with 0.25 volumes of the cDNA library solution and then digested with 1 μ L of NEBNext USER enzyme at 37°C for 15 minutes. .. The purified cDNA library was analyzed along with Quick-Load 1 kb Extended DNA Ladder (New England Biolabs, Ipswich, MA) by electrophoresis (2% agarose gel) followed by ethidium bromide staining to visualize the optimal size range of the cDNA library (250 bp–400 bp).

    Flow Cytometry:

    Article Title: Benchmarking of the Oxford Nanopore MinION sequencing for quantitative and qualitative assessment of cDNA populations
    Article Snippet: For the subsequent dA-tailing step all the eluted DNA was used in the following reaction: cDNA library solution from the previous step ( > 750 ng), 3 ul 10x dA-tailing buffer (from the NEBNext dA-Tailing Module), 2 ul dA-tailing enzyme (from the NEBNext dA-Tailing Module). .. Depending on the protocol the cDNA library was either loaded on the flow cell as is (SQK-MAP0003 kit version) or enriched for sequences that bear the hairpin (SQK-MAP0004, SQK-MAP0005 kit version) as follows.10 ul of His-beads (Dynabeads His-Tag, Thermo) were washed two times with 1x Bead Binding Buffer (SQK-MAP0005 kit), then resuspended in 100 μl of the 2x Bead Binding Buffer (SQK-MAP0005 kit) and added to the adaptor ligated DNA.

    Selection:

    Article Title: Transcriptome Analysis of Gelatin Seed Treatment as a Biostimulant of Cucumber Plant Growth
    Article Snippet: .. Another round of size selection was performed with 0.25 volumes of the cDNA library solution and then digested with 1 μ L of NEBNext USER enzyme at 37°C for 15 minutes. .. The cDNA library was enriched by PCR with NEBNext index primers following conditions, 98°C for 30 seconds; 15 cycles of 98°C for 10 seconds, 65°C for 30 seconds, and 72°C for 30 seconds; 72°C for 5 minutes, and then held at 4°C.

    RNA Sequencing Assay:

    Article Title: Transcriptome Analysis of Gelatin Seed Treatment as a Biostimulant of Cucumber Plant Growth
    Article Snippet: Paragraph title: 2.2. RNA Isolation and Strand-Specific RNA-seq Library Construction and Sequencing ... Another round of size selection was performed with 0.25 volumes of the cDNA library solution and then digested with 1 μ L of NEBNext USER enzyme at 37°C for 15 minutes.

    Synthesized:

    Article Title: Transcriptome Analysis of Gelatin Seed Treatment as a Biostimulant of Cucumber Plant Growth
    Article Snippet: The synthesized first strand cDNA was then used as a template to synthesize double stranded cDNA. .. Another round of size selection was performed with 0.25 volumes of the cDNA library solution and then digested with 1 μ L of NEBNext USER enzyme at 37°C for 15 minutes.

    Isolation:

    Article Title: Benchmarking of the Oxford Nanopore MinION sequencing for quantitative and qualitative assessment of cDNA populations
    Article Snippet: The isolated DNA was quantified on the Qubit fluorimeter. .. For the subsequent dA-tailing step all the eluted DNA was used in the following reaction: cDNA library solution from the previous step ( > 750 ng), 3 ul 10x dA-tailing buffer (from the NEBNext dA-Tailing Module), 2 ul dA-tailing enzyme (from the NEBNext dA-Tailing Module).

    Article Title: Transcriptome Analysis of Gelatin Seed Treatment as a Biostimulant of Cucumber Plant Growth
    Article Snippet: Paragraph title: 2.2. RNA Isolation and Strand-Specific RNA-seq Library Construction and Sequencing ... Another round of size selection was performed with 0.25 volumes of the cDNA library solution and then digested with 1 μ L of NEBNext USER enzyme at 37°C for 15 minutes.

    Ligation:

    Article Title: Benchmarking of the Oxford Nanopore MinION sequencing for quantitative and qualitative assessment of cDNA populations
    Article Snippet: For the subsequent dA-tailing step all the eluted DNA was used in the following reaction: cDNA library solution from the previous step ( > 750 ng), 3 ul 10x dA-tailing buffer (from the NEBNext dA-Tailing Module), 2 ul dA-tailing enzyme (from the NEBNext dA-Tailing Module). .. Subsequently, depending on the protocol version the DNA was cleaned with the Ampure XP beads as described above (SQK-MAP0005 kit version) or used immediately in the ligation reaction (SQK-MAP0004, SQK-MAP0003 kit version).

    Article Title: Transcriptome Analysis of Gelatin Seed Treatment as a Biostimulant of Cucumber Plant Growth
    Article Snippet: Small size (approximately 300 bp) fragments were selected using Agencourt AMPure XP beads (Bechman Coulter, Pasadena, CA) in 0.6 volumes of ligation reaction. .. Another round of size selection was performed with 0.25 volumes of the cDNA library solution and then digested with 1 μ L of NEBNext USER enzyme at 37°C for 15 minutes.

    Purification:

    Article Title: Transcriptome Analysis of Gelatin Seed Treatment as a Biostimulant of Cucumber Plant Growth
    Article Snippet: Another round of size selection was performed with 0.25 volumes of the cDNA library solution and then digested with 1 μ L of NEBNext USER enzyme at 37°C for 15 minutes. .. The PCR-enriched cDNA libraries were purified by 1.4 volumes of Agencourt AMPure XP beads and eluted in 22 μ L Elution Buffer.

    Electrophoresis:

    Article Title: Transcriptome Analysis of Gelatin Seed Treatment as a Biostimulant of Cucumber Plant Growth
    Article Snippet: Another round of size selection was performed with 0.25 volumes of the cDNA library solution and then digested with 1 μ L of NEBNext USER enzyme at 37°C for 15 minutes. .. The purified cDNA library was analyzed along with Quick-Load 1 kb Extended DNA Ladder (New England Biolabs, Ipswich, MA) by electrophoresis (2% agarose gel) followed by ethidium bromide staining to visualize the optimal size range of the cDNA library (250 bp–400 bp).

    Polymerase Chain Reaction:

    Article Title: Transcriptome Analysis of Gelatin Seed Treatment as a Biostimulant of Cucumber Plant Growth
    Article Snippet: Another round of size selection was performed with 0.25 volumes of the cDNA library solution and then digested with 1 μ L of NEBNext USER enzyme at 37°C for 15 minutes. .. The cDNA library was enriched by PCR with NEBNext index primers following conditions, 98°C for 30 seconds; 15 cycles of 98°C for 10 seconds, 65°C for 30 seconds, and 72°C for 30 seconds; 72°C for 5 minutes, and then held at 4°C.

    cDNA Library Assay:

    Article Title: Benchmarking of the Oxford Nanopore MinION sequencing for quantitative and qualitative assessment of cDNA populations
    Article Snippet: .. For the subsequent dA-tailing step all the eluted DNA was used in the following reaction: cDNA library solution from the previous step ( > 750 ng), 3 ul 10x dA-tailing buffer (from the NEBNext dA-Tailing Module), 2 ul dA-tailing enzyme (from the NEBNext dA-Tailing Module). .. Subsequently, depending on the protocol version the DNA was cleaned with the Ampure XP beads as described above (SQK-MAP0005 kit version) or used immediately in the ligation reaction (SQK-MAP0004, SQK-MAP0003 kit version).

    Article Title: Transcriptome Analysis of Gelatin Seed Treatment as a Biostimulant of Cucumber Plant Growth
    Article Snippet: .. Another round of size selection was performed with 0.25 volumes of the cDNA library solution and then digested with 1 μ L of NEBNext USER enzyme at 37°C for 15 minutes. .. The cDNA library was enriched by PCR with NEBNext index primers following conditions, 98°C for 30 seconds; 15 cycles of 98°C for 10 seconds, 65°C for 30 seconds, and 72°C for 30 seconds; 72°C for 5 minutes, and then held at 4°C.

    Sequencing:

    Article Title: Benchmarking of the Oxford Nanopore MinION sequencing for quantitative and qualitative assessment of cDNA populations
    Article Snippet: Paragraph title: ONT MinION sequencing of the ERCC cDNA population ... For the subsequent dA-tailing step all the eluted DNA was used in the following reaction: cDNA library solution from the previous step ( > 750 ng), 3 ul 10x dA-tailing buffer (from the NEBNext dA-Tailing Module), 2 ul dA-tailing enzyme (from the NEBNext dA-Tailing Module).

    Article Title: Transcriptome Analysis of Gelatin Seed Treatment as a Biostimulant of Cucumber Plant Growth
    Article Snippet: Paragraph title: 2.2. RNA Isolation and Strand-Specific RNA-seq Library Construction and Sequencing ... Another round of size selection was performed with 0.25 volumes of the cDNA library solution and then digested with 1 μ L of NEBNext USER enzyme at 37°C for 15 minutes.

    Staining:

    Article Title: Transcriptome Analysis of Gelatin Seed Treatment as a Biostimulant of Cucumber Plant Growth
    Article Snippet: Another round of size selection was performed with 0.25 volumes of the cDNA library solution and then digested with 1 μ L of NEBNext USER enzyme at 37°C for 15 minutes. .. The purified cDNA library was analyzed along with Quick-Load 1 kb Extended DNA Ladder (New England Biolabs, Ipswich, MA) by electrophoresis (2% agarose gel) followed by ethidium bromide staining to visualize the optimal size range of the cDNA library (250 bp–400 bp).

    Binding Assay:

    Article Title: Benchmarking of the Oxford Nanopore MinION sequencing for quantitative and qualitative assessment of cDNA populations
    Article Snippet: For the subsequent dA-tailing step all the eluted DNA was used in the following reaction: cDNA library solution from the previous step ( > 750 ng), 3 ul 10x dA-tailing buffer (from the NEBNext dA-Tailing Module), 2 ul dA-tailing enzyme (from the NEBNext dA-Tailing Module). .. Depending on the protocol the cDNA library was either loaded on the flow cell as is (SQK-MAP0003 kit version) or enriched for sequences that bear the hairpin (SQK-MAP0004, SQK-MAP0005 kit version) as follows.10 ul of His-beads (Dynabeads His-Tag, Thermo) were washed two times with 1x Bead Binding Buffer (SQK-MAP0005 kit), then resuspended in 100 μl of the 2x Bead Binding Buffer (SQK-MAP0005 kit) and added to the adaptor ligated DNA.

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  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    New England Biolabs cdna library solution
    Cdna Library Solution, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cdna library solution/product/New England Biolabs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cdna library solution - by Bioz Stars, 2020-02
    92/100 stars
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