Journal: Molecules
Article Title: Cytotoxicity of a Lipid-Rich Extract from Native Mexican Avocado Seed ( Persea americana var. drymifolia) on Canine Osteosarcoma D-17 Cells and Synergistic Activity with Cytostatic Drugs
doi: 10.3390/molecules26144178
Figure Lengend Snippet: Synergistic effect of LEAS with cytostatic drugs on cell viability of D-17 cells. ( A ) Cisplatin, ( B ) Carboplatin, and ( C ) Doxorubicin. D-17 cells were subjected to the simultaneous treatment with LEAS (5, 10, 20, 50 µg/mL), cisplatin (3.34, 16.7, 33.4, 66.8 μM), carboplatin (50, 100, 200, 400 μM) or doxorubicin (46.8, 97.7, 187, 350 nM) and incubated for 72 h. The viable cells were determined by MTT assay and the IC 50 was estimated. Controls consisting of cells treated with the individual compounds were included. All treatments were evaluated in two independent experiments performed by triplicate.
Article Snippet: Cell cycle analysis was performed in D-17 cell line treated with LEAS using the BD cycle test Plus DNA kit (BD biosciences, San Jose, CA, USA) according to manufacturer’s specifications.
Techniques: Incubation, MTT Assay