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d 17 ccl 183 cell line  (ATCC)


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    ATCC d 17 ccl 183 cell line
    D 17 Ccl 183 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/d 17 ccl 183 cell line/product/ATCC
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    d 17 ccl 183 cell line - by Bioz Stars, 2024-11
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    Effect of LEAS on canine osteosarcoma <t>D-17</t> cell line viability. Cells were treated with LEAS (1, 5, 10, 20, 50, 75, and 100 μg/mL) and cell viability was evaluated by MTT assays at 24 h ( A ) and 48 h ( B ). Cell viability is shown with respect to cells treated with vehicle (DMSO 0.1%). Actinomycin D (ActD) was used as a positive control (80 μg/mL). Data represent the mean of three independent experiments performed in triplicate. Different letters denote significant differences within the treatments (one-way ANOVA and Tukey’s pairwise comparison, p < 0.05). ( C ) Linear regression analysis of the concentration-response to calculate the half-maximal inhibitory concentration (IC 50 ) of LEAS on the D-17 cell line at 48 h. ( D ) In vitro assessment of LEAS IC 50 (15.5 μg/mL) by flow cytometry at 48 h using SYTO TM 9 green-fluorescent nucleic acid stain and propidium iodide. Representative plots of different treatments are shown.
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    ATCC canine osteosarcoma cell line d 17
    Effect of LEAS on canine osteosarcoma <t>D-17</t> cell line viability. Cells were treated with LEAS (1, 5, 10, 20, 50, 75, and 100 μg/mL) and cell viability was evaluated by MTT assays at 24 h ( A ) and 48 h ( B ). Cell viability is shown with respect to cells treated with vehicle (DMSO 0.1%). Actinomycin D (ActD) was used as a positive control (80 μg/mL). Data represent the mean of three independent experiments performed in triplicate. Different letters denote significant differences within the treatments (one-way ANOVA and Tukey’s pairwise comparison, p < 0.05). ( C ) Linear regression analysis of the concentration-response to calculate the half-maximal inhibitory concentration (IC 50 ) of LEAS on the D-17 cell line at 48 h. ( D ) In vitro assessment of LEAS IC 50 (15.5 μg/mL) by flow cytometry at 48 h using SYTO TM 9 green-fluorescent nucleic acid stain and propidium iodide. Representative plots of different treatments are shown.
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    Effect of LEAS on canine osteosarcoma D-17 cell line viability. Cells were treated with LEAS (1, 5, 10, 20, 50, 75, and 100 μg/mL) and cell viability was evaluated by MTT assays at 24 h ( A ) and 48 h ( B ). Cell viability is shown with respect to cells treated with vehicle (DMSO 0.1%). Actinomycin D (ActD) was used as a positive control (80 μg/mL). Data represent the mean of three independent experiments performed in triplicate. Different letters denote significant differences within the treatments (one-way ANOVA and Tukey’s pairwise comparison, p < 0.05). ( C ) Linear regression analysis of the concentration-response to calculate the half-maximal inhibitory concentration (IC 50 ) of LEAS on the D-17 cell line at 48 h. ( D ) In vitro assessment of LEAS IC 50 (15.5 μg/mL) by flow cytometry at 48 h using SYTO TM 9 green-fluorescent nucleic acid stain and propidium iodide. Representative plots of different treatments are shown.

    Journal: Molecules

    Article Title: Cytotoxicity of a Lipid-Rich Extract from Native Mexican Avocado Seed ( Persea americana var. drymifolia) on Canine Osteosarcoma D-17 Cells and Synergistic Activity with Cytostatic Drugs

    doi: 10.3390/molecules26144178

    Figure Lengend Snippet: Effect of LEAS on canine osteosarcoma D-17 cell line viability. Cells were treated with LEAS (1, 5, 10, 20, 50, 75, and 100 μg/mL) and cell viability was evaluated by MTT assays at 24 h ( A ) and 48 h ( B ). Cell viability is shown with respect to cells treated with vehicle (DMSO 0.1%). Actinomycin D (ActD) was used as a positive control (80 μg/mL). Data represent the mean of three independent experiments performed in triplicate. Different letters denote significant differences within the treatments (one-way ANOVA and Tukey’s pairwise comparison, p < 0.05). ( C ) Linear regression analysis of the concentration-response to calculate the half-maximal inhibitory concentration (IC 50 ) of LEAS on the D-17 cell line at 48 h. ( D ) In vitro assessment of LEAS IC 50 (15.5 μg/mL) by flow cytometry at 48 h using SYTO TM 9 green-fluorescent nucleic acid stain and propidium iodide. Representative plots of different treatments are shown.

    Article Snippet: Cell cycle analysis was performed in D-17 cell line treated with LEAS using the BD cycle test Plus DNA kit (BD biosciences, San Jose, CA, USA) according to manufacturer’s specifications.

    Techniques: Positive Control, Concentration Assay, In Vitro, Flow Cytometry, Staining

    LEAS does not affect the cytoplasmic membrane integrity in D-17 cells. ( A ) Changes in the cytoplasmic membrane potential of D-17 cells were evaluated using membrane potential-sensitive dye. Cells were previously incubated with 200 μM of the dye DiSC3(5) for 30 min at 37 °C and then treated with LEAS IC 50 (15.5 µg/mL). Valinomycin (0.2 mM) was used as a positive control. ( B ) The efflux of cytosolic calcium was analyzed by flow cytometry. Measurements were assayed by 3 min, and at minute one the cells were treated with LEAS IC 50 (15.5 µg/mL), vehicle, or PMA 3 μM (acetate phorbol myristate) as a positive control. Representative plots are shown. Arrows indicate the time at which the treatments were added. Vehicle = DMSO (0.1%).

    Journal: Molecules

    Article Title: Cytotoxicity of a Lipid-Rich Extract from Native Mexican Avocado Seed ( Persea americana var. drymifolia) on Canine Osteosarcoma D-17 Cells and Synergistic Activity with Cytostatic Drugs

    doi: 10.3390/molecules26144178

    Figure Lengend Snippet: LEAS does not affect the cytoplasmic membrane integrity in D-17 cells. ( A ) Changes in the cytoplasmic membrane potential of D-17 cells were evaluated using membrane potential-sensitive dye. Cells were previously incubated with 200 μM of the dye DiSC3(5) for 30 min at 37 °C and then treated with LEAS IC 50 (15.5 µg/mL). Valinomycin (0.2 mM) was used as a positive control. ( B ) The efflux of cytosolic calcium was analyzed by flow cytometry. Measurements were assayed by 3 min, and at minute one the cells were treated with LEAS IC 50 (15.5 µg/mL), vehicle, or PMA 3 μM (acetate phorbol myristate) as a positive control. Representative plots are shown. Arrows indicate the time at which the treatments were added. Vehicle = DMSO (0.1%).

    Article Snippet: Cell cycle analysis was performed in D-17 cell line treated with LEAS using the BD cycle test Plus DNA kit (BD biosciences, San Jose, CA, USA) according to manufacturer’s specifications.

    Techniques: Incubation, Positive Control, Flow Cytometry

    Apoptosis induced by LEAS in D-17 cells. ( A ) Cells treated with LEAS IC 50 (15.5 µg/mL) for 24 h and 48 h were analyzed by flow cytometry using Annexin-V and /AAD staining to establish the apoptotic rate. Cells sorted into quadrants Q1, Q2, Q3 and, Q4 represent necrotic, late apoptotic, early apoptotic, and viable (live) populations, respectively. Representative plots are shown. ( B ) The graphic shows the apoptosis fold-change in the treatments at times evaluated. Each bar shows the mean of triplicates ± SE. A minimum of 10,000 events per sample was collected. * and ** indicate statistically significant differences with respect to vehicles ( p < 0.05) at the different times evaluated. ActD = Actinomycin D (80 μg/mL). Veh = vehicle (DMSO 0.1%).

    Journal: Molecules

    Article Title: Cytotoxicity of a Lipid-Rich Extract from Native Mexican Avocado Seed ( Persea americana var. drymifolia) on Canine Osteosarcoma D-17 Cells and Synergistic Activity with Cytostatic Drugs

    doi: 10.3390/molecules26144178

    Figure Lengend Snippet: Apoptosis induced by LEAS in D-17 cells. ( A ) Cells treated with LEAS IC 50 (15.5 µg/mL) for 24 h and 48 h were analyzed by flow cytometry using Annexin-V and /AAD staining to establish the apoptotic rate. Cells sorted into quadrants Q1, Q2, Q3 and, Q4 represent necrotic, late apoptotic, early apoptotic, and viable (live) populations, respectively. Representative plots are shown. ( B ) The graphic shows the apoptosis fold-change in the treatments at times evaluated. Each bar shows the mean of triplicates ± SE. A minimum of 10,000 events per sample was collected. * and ** indicate statistically significant differences with respect to vehicles ( p < 0.05) at the different times evaluated. ActD = Actinomycin D (80 μg/mL). Veh = vehicle (DMSO 0.1%).

    Article Snippet: Cell cycle analysis was performed in D-17 cell line treated with LEAS using the BD cycle test Plus DNA kit (BD biosciences, San Jose, CA, USA) according to manufacturer’s specifications.

    Techniques: Flow Cytometry, Staining

    Caspase-8 and caspase-9 activation by LEAS in D-17 cells. Cells treated with LEAS IC 50 (15.5 μg/mL) for 24 h and 48 h were analyzed by flow cytometry to caspase-8 ( A ) and caspase-9 activation ( B ). Representative plots are shown. The graphic shows percentage of cells with caspase-activated for each time of treatment. Each bar shows the mean of triplicates ± SE of two independent experiments. Different letters denote significant differences within the treatments at the time evaluated (one-way ANOVA and Tukey’s pairwise comparison, p < 0.05). ActD = Actinomycin D (80 μg/mL). Veh = vehicle (DMSO 0.1%).

    Journal: Molecules

    Article Title: Cytotoxicity of a Lipid-Rich Extract from Native Mexican Avocado Seed ( Persea americana var. drymifolia) on Canine Osteosarcoma D-17 Cells and Synergistic Activity with Cytostatic Drugs

    doi: 10.3390/molecules26144178

    Figure Lengend Snippet: Caspase-8 and caspase-9 activation by LEAS in D-17 cells. Cells treated with LEAS IC 50 (15.5 μg/mL) for 24 h and 48 h were analyzed by flow cytometry to caspase-8 ( A ) and caspase-9 activation ( B ). Representative plots are shown. The graphic shows percentage of cells with caspase-activated for each time of treatment. Each bar shows the mean of triplicates ± SE of two independent experiments. Different letters denote significant differences within the treatments at the time evaluated (one-way ANOVA and Tukey’s pairwise comparison, p < 0.05). ActD = Actinomycin D (80 μg/mL). Veh = vehicle (DMSO 0.1%).

    Article Snippet: Cell cycle analysis was performed in D-17 cell line treated with LEAS using the BD cycle test Plus DNA kit (BD biosciences, San Jose, CA, USA) according to manufacturer’s specifications.

    Techniques: Activation Assay, Flow Cytometry

    LEAS generates the loss of mitochondrial membrane potential (ΔΨm) in D-17 cells. ( A ) The mitochondrial membrane potential was evaluated by flow cytometry using the JC-1 dye. Cells were treated for 12 h and 24 h with LEAS IC 50 (15.5 μg/mL), vehicle, or Act D. Representative plots are shown. ( B ) The graphic shows fold-change values for each time of treatment. Each bar shows the mean of triplicates ± SE of two independent experiments. “*” and “**” Indicates statistically significant differences with relation to the vehicle at the time evaluated ( p < 0.05). ActD = Actinomycin D (80 μg/mL). Veh = vehicle (DMSO 0.1%).

    Journal: Molecules

    Article Title: Cytotoxicity of a Lipid-Rich Extract from Native Mexican Avocado Seed ( Persea americana var. drymifolia) on Canine Osteosarcoma D-17 Cells and Synergistic Activity with Cytostatic Drugs

    doi: 10.3390/molecules26144178

    Figure Lengend Snippet: LEAS generates the loss of mitochondrial membrane potential (ΔΨm) in D-17 cells. ( A ) The mitochondrial membrane potential was evaluated by flow cytometry using the JC-1 dye. Cells were treated for 12 h and 24 h with LEAS IC 50 (15.5 μg/mL), vehicle, or Act D. Representative plots are shown. ( B ) The graphic shows fold-change values for each time of treatment. Each bar shows the mean of triplicates ± SE of two independent experiments. “*” and “**” Indicates statistically significant differences with relation to the vehicle at the time evaluated ( p < 0.05). ActD = Actinomycin D (80 μg/mL). Veh = vehicle (DMSO 0.1%).

    Article Snippet: Cell cycle analysis was performed in D-17 cell line treated with LEAS using the BD cycle test Plus DNA kit (BD biosciences, San Jose, CA, USA) according to manufacturer’s specifications.

    Techniques: Flow Cytometry

    LEAS increased the production of ROS in D-17 cells. The production of mitochondrial ROS ( A ) and superoxide anion ( B ) were assessed by flow cytometry using the dyes DHE (5 μM) and DHR (10 μM), respectively. Cells were treated for 48 h with LEAS IC 50 (15.5 μg/mL), vehicle (DMSO 0.1%), or ethanol (12%) as a positive control. The graphic shows fold-change values for each treatment. Each bar shows the mean of triplicates ± SE of two independent experiments. Different letters indicate statistically significant differences in relation to the vehicle ( p < 0.05).

    Journal: Molecules

    Article Title: Cytotoxicity of a Lipid-Rich Extract from Native Mexican Avocado Seed ( Persea americana var. drymifolia) on Canine Osteosarcoma D-17 Cells and Synergistic Activity with Cytostatic Drugs

    doi: 10.3390/molecules26144178

    Figure Lengend Snippet: LEAS increased the production of ROS in D-17 cells. The production of mitochondrial ROS ( A ) and superoxide anion ( B ) were assessed by flow cytometry using the dyes DHE (5 μM) and DHR (10 μM), respectively. Cells were treated for 48 h with LEAS IC 50 (15.5 μg/mL), vehicle (DMSO 0.1%), or ethanol (12%) as a positive control. The graphic shows fold-change values for each treatment. Each bar shows the mean of triplicates ± SE of two independent experiments. Different letters indicate statistically significant differences in relation to the vehicle ( p < 0.05).

    Article Snippet: Cell cycle analysis was performed in D-17 cell line treated with LEAS using the BD cycle test Plus DNA kit (BD biosciences, San Jose, CA, USA) according to manufacturer’s specifications.

    Techniques: Flow Cytometry, Positive Control

    Synergistic effect of LEAS with cytostatic drugs on cell viability of D-17 cells. ( A ) Cisplatin, ( B ) Carboplatin, and ( C ) Doxorubicin. D-17 cells were subjected to the simultaneous treatment with LEAS (5, 10, 20, 50 µg/mL), cisplatin (3.34, 16.7, 33.4, 66.8 μM), carboplatin (50, 100, 200, 400 μM) or doxorubicin (46.8, 97.7, 187, 350 nM) and incubated for 72 h. The viable cells were determined by MTT assay and the IC 50 was estimated. Controls consisting of cells treated with the individual compounds were included. All treatments were evaluated in two independent experiments performed by triplicate.

    Journal: Molecules

    Article Title: Cytotoxicity of a Lipid-Rich Extract from Native Mexican Avocado Seed ( Persea americana var. drymifolia) on Canine Osteosarcoma D-17 Cells and Synergistic Activity with Cytostatic Drugs

    doi: 10.3390/molecules26144178

    Figure Lengend Snippet: Synergistic effect of LEAS with cytostatic drugs on cell viability of D-17 cells. ( A ) Cisplatin, ( B ) Carboplatin, and ( C ) Doxorubicin. D-17 cells were subjected to the simultaneous treatment with LEAS (5, 10, 20, 50 µg/mL), cisplatin (3.34, 16.7, 33.4, 66.8 μM), carboplatin (50, 100, 200, 400 μM) or doxorubicin (46.8, 97.7, 187, 350 nM) and incubated for 72 h. The viable cells were determined by MTT assay and the IC 50 was estimated. Controls consisting of cells treated with the individual compounds were included. All treatments were evaluated in two independent experiments performed by triplicate.

    Article Snippet: Cell cycle analysis was performed in D-17 cell line treated with LEAS using the BD cycle test Plus DNA kit (BD biosciences, San Jose, CA, USA) according to manufacturer’s specifications.

    Techniques: Incubation, MTT Assay