d 17 ccl 183 cell line  (ATCC)

Journal: Veterinary Medicine and Science

Article Title: Enhanced expression of cyclooxygenase‐2 related multi‐drug resistance gene in melanoma and osteosarcoma cell lines by TSG‐6 secreted from canine adipose‐derived mesenchymal stem/stromal cells

doi: 10.1002/vms3.442

Figure Lengend Snippet: cADSCs increased COX‐2 expression in cancer cell lines. Co‐culture with cADSCs increased COX‐2 protein levels of (a) canine melanoma (LMeC) and (b) osteosarcoma (D17) cell lines. The COX‐2 protein level increased as the proportion of cADSC increased. Experiments were performed in triplicate and repeated three times with similar results. Results are shown as mean ± SD (* p < .05, ** p < .01, *** p < .001 by one‐way ANOVA analysis)

Article Snippet: Canine malignant melanoma cell line LMeC (RRID: CVCL_L361) was provided by Professor Nobuo Sasaki, University of Tokyo. (Inoue et al., ( )) Canine osteosarcoma cell line D17 [American Type Culture Collection (ATCC) CCL‐183] was obtained from the Laboratory of Veterinary Pharmacology, Seoul National University.

Techniques: Expressing, Co-Culture Assay

Journal: Veterinary Medicine and Science

Article Title: Enhanced expression of cyclooxygenase‐2 related multi‐drug resistance gene in melanoma and osteosarcoma cell lines by TSG‐6 secreted from canine adipose‐derived mesenchymal stem/stromal cells

doi: 10.1002/vms3.442

Figure Lengend Snippet: Drug resistance gene expression in cancer cell lines increased in the presence of cADSCs, and decreased with interference of COX‐2. COX‐2 protein levels in LMeC (a) and D17 (b) cancer cell lines. mRNA expression levels of COX‐2, MRP1, P‐gp in LMeC (c) and D17 (d) cancer cell lines. COX‐2 and drug resistance gene MRP1, P‐gp expression of cancer cell lines increased significantly with the presence of cADSCs . When COX‐2 was down regulated with COX‐2 targeted siRNA (siCOX‐2) in cancer cell lines, MRP1 and P‐gp expression in LMeC, D17 cells was decreased even when cultured with cADSCs. Experiments were performed in triplicate and repeated three times with similar results. Results are shown as mean ± standard deviation (* p < .05, ** p < .01, *** p < .001 by one‐way ANOVA analysis)

Article Snippet: Canine malignant melanoma cell line LMeC (RRID: CVCL_L361) was provided by Professor Nobuo Sasaki, University of Tokyo. (Inoue et al., ( )) Canine osteosarcoma cell line D17 [American Type Culture Collection (ATCC) CCL‐183] was obtained from the Laboratory of Veterinary Pharmacology, Seoul National University.

Techniques: Expressing, Cell Culture, Standard Deviation

Journal: Veterinary Medicine and Science

Article Title: Enhanced expression of cyclooxygenase‐2 related multi‐drug resistance gene in melanoma and osteosarcoma cell lines by TSG‐6 secreted from canine adipose‐derived mesenchymal stem/stromal cells

doi: 10.1002/vms3.442

Figure Lengend Snippet: TSG‐6 secreted from cADSC elevated the expression of COX‐2 in cancer cell lines. mRNA expression levels of COX‐2 in LMeC (a), and D17 (b) cell lines. Protein levels of COX‐2 in LMeC (c) and D17 (d) cell lines. COX‐2 mRNA and protein expression in cancer cell lines decreased significantly when they were co‐cultured with TSG‐6 targeted siRNA (siTSG‐6) transfected cADSCs. Experiments were performed in triplicate and repeated three times with similar results. Results are shown as mean ± SD (* p < .05, ** p < .01, *** p < .001 by one‐way ANOVA analysis)

Article Snippet: Canine malignant melanoma cell line LMeC (RRID: CVCL_L361) was provided by Professor Nobuo Sasaki, University of Tokyo. (Inoue et al., ( )) Canine osteosarcoma cell line D17 [American Type Culture Collection (ATCC) CCL‐183] was obtained from the Laboratory of Veterinary Pharmacology, Seoul National University.

Techniques: Expressing, Cell Culture, Transfection

Journal: Veterinary Medicine and Science

Article Title: Enhanced expression of cyclooxygenase‐2 related multi‐drug resistance gene in melanoma and osteosarcoma cell lines by TSG‐6 secreted from canine adipose‐derived mesenchymal stem/stromal cells

doi: 10.1002/vms3.442

Figure Lengend Snippet: TSG‐6 secreted from cADSCs affected drug resistance genes in cancer cell lines. mRNA expression levels of MRP1 and P‐gp in LMeC (a) and D17 (b) cell lines. Experiments were performed in triplicate and repeated three times with similar results. Results are shown as mean ± standard deviation (* p < .05, ** p < .01, *** p < .001 by one‐way ANOVA analysis)

Article Snippet: Canine malignant melanoma cell line LMeC (RRID: CVCL_L361) was provided by Professor Nobuo Sasaki, University of Tokyo. (Inoue et al., ( )) Canine osteosarcoma cell line D17 [American Type Culture Collection (ATCC) CCL‐183] was obtained from the Laboratory of Veterinary Pharmacology, Seoul National University.

Techniques: Expressing, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Anticancer Effects of Cold Atmospheric Plasma in Canine Osteosarcoma Cells

doi: 10.3390/ijms21124556

Figure Lengend Snippet: Induction of cell growth arrest by cold atmospheric plasma. ( A ) Representative images of the D-17 and DSN canine osteosarcoma cell lines treated with CAP. Scale bars, 100 μm. ( B ) Cell viability assay. D-17 and DSN cells were exposed to CAP at the indicated time. Cell viability was measured using CellTiter 96 AQ ueous One Solution. Error bars represent the mean ± S.E.M. of three replicates. ( C ). Measurement of de novo DNA synthesis. D-17 cells were stained with 5-ethynyl-2′-deoxyuridine (EdU), which stains the newly synthesized DNA, and the intensity was quantified by high-content screening technology. Green, EdU; and Blue, Hoechest 33342. Error bars represent the mean ± S.E.M. of three replicates. Magnification, 100X. * p < 0.05, ** p < 0.01, *** p < 0.001; N.S. indicates not significant.

Article Snippet: The DSN canine osteosarcoma cell line (ATCC CRL-9939) was generously provided by Dr. Gwonhwa Song [ , ], and the D-17 cell line (ATCC CCL-183) was used as previously reported [ ].

Techniques: Viability Assay, DNA Synthesis, Staining, Synthesized, High Content Screening

Journal: International Journal of Molecular Sciences

Article Title: Anticancer Effects of Cold Atmospheric Plasma in Canine Osteosarcoma Cells

doi: 10.3390/ijms21124556

Figure Lengend Snippet: Reactive oxygen species (ROS) generation and DNA damage after exposure to cold atmospheric plasma (CAP). ( A ) Representative images of the canine osteosarcoma D-17 cell line after exposure to CAP at the indicated time. CAP-treated cells were stained with H 2 DCFDA and photographed under a fluorescence microscope. H 2 DCFDA is converted to DCF by ROS. DCF, 2′,7′-dichlorodihydrofluorescein. Scale bars, 50 μm. ( B ) Quantitative analysis of ROS using high-content screening. CAP-treated D-17 cells stained with H 2 DCFDA and Hoechst 33342 were measured by high-content screening technology. Error bars represent the mean ± S.E.M. of three replicates. Magnification, 100X. ( C ) Western blot analysis of DNA damage. Expression of phospho-histone-H2A.X was measured to assess DNA damage. This result represents two independent experiments. The intensity was normalized to β-actin. *** p < 0.001; N.S. indicates not significant.

Article Snippet: The DSN canine osteosarcoma cell line (ATCC CRL-9939) was generously provided by Dr. Gwonhwa Song [ , ], and the D-17 cell line (ATCC CCL-183) was used as previously reported [ ].

Techniques: Staining, Fluorescence, Microscopy, High Content Screening, Western Blot, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Anticancer Effects of Cold Atmospheric Plasma in Canine Osteosarcoma Cells

doi: 10.3390/ijms21124556

Figure Lengend Snippet: Migration and invasion activity of D-17 cells after exposure to cold atmospheric plasma (CAP). ( A ) Cell migration assay. Representative images of cells before and after exposure to CAP. Magnification, 40X. Quantitative analysis of the area refilled after 48 h. The graph represents three independent experiments. Error bars represent the mean ± S.E.M. ( B ) Three-dimensional tumor spheroid invasion assay. Representative images of spheroids before and after exposure to CAP. Invadopodia were observed in control and gas-treated group (black arrow). Scale bar, 100 μm. Volume of the control or CAP-treated spheroids was measured and is presented as a graph. Error bars represent the mean ± S.E.M. of three replicates. * p < 0.05, ** p < 0.01, *** p < 0.001, compared to control; N.S. indicates not significant.

Article Snippet: The DSN canine osteosarcoma cell line (ATCC CRL-9939) was generously provided by Dr. Gwonhwa Song [ , ], and the D-17 cell line (ATCC CCL-183) was used as previously reported [ ].

Techniques: Migration, Activity Assay, Cell Migration Assay, Invasion Assay