cytokine singleplex assays  (Thermo Fisher)


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    Structured Review

    Thermo Fisher cytokine singleplex assays
    Cytokine Singleplex Assays, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cytokine singleplex assays/product/Thermo Fisher
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cytokine singleplex assays - by Bioz Stars, 2020-04
    84/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Natural killer cells are recruited during pulmonary tuberculosis and their ex vivo responses to mycobacteria vary between healthy human donors in association with KIR haplotype
    Article Snippet: .. Cytokine production analysis Cell culture supernatants were filtered using 0.2 μm 96-well filter plates (Corning) before detection of cytokines and chemokines using either ELISA kits (Peprotech) or combined cytokine singleplex assays (Invitrogen) on a Luminex100 , χMAPTM Technology. .. For intracellular cytokine detection, NK cells were stimulated for 24 h. Brefeldin A (BioLegend) was added during the last 6 h of culture before harvesting cells for antibody staining with anti-CD16-FITC (Miltenyi Biotec) and anti-CD56-PC7 (BD Bioscience), then fixed and permeabilized using BD Cytofix/Cytoperm™ buffer and stained with anti-IFNγ-PE (BD Biosciences).

    Staining:

    Article Title: Natural killer cells are recruited during pulmonary tuberculosis and their ex vivo responses to mycobacteria vary between healthy human donors in association with KIR haplotype
    Article Snippet: Cytokine production analysis Cell culture supernatants were filtered using 0.2 μm 96-well filter plates (Corning) before detection of cytokines and chemokines using either ELISA kits (Peprotech) or combined cytokine singleplex assays (Invitrogen) on a Luminex100 , χMAPTM Technology. .. For intracellular cytokine detection, NK cells were stimulated for 24 h. Brefeldin A (BioLegend) was added during the last 6 h of culture before harvesting cells for antibody staining with anti-CD16-FITC (Miltenyi Biotec) and anti-CD56-PC7 (BD Bioscience), then fixed and permeabilized using BD Cytofix/Cytoperm™ buffer and stained with anti-IFNγ-PE (BD Biosciences).

    Flow Cytometry:

    Article Title: Natural killer cells are recruited during pulmonary tuberculosis and their ex vivo responses to mycobacteria vary between healthy human donors in association with KIR haplotype
    Article Snippet: Cytokine production analysis Cell culture supernatants were filtered using 0.2 μm 96-well filter plates (Corning) before detection of cytokines and chemokines using either ELISA kits (Peprotech) or combined cytokine singleplex assays (Invitrogen) on a Luminex100 , χMAPTM Technology. .. Cells were run on a BD Biosciences FACSCalibur flow cytometer and data analysed using FlowJo 7.6.4.

    Cytometry:

    Article Title: Natural killer cells are recruited during pulmonary tuberculosis and their ex vivo responses to mycobacteria vary between healthy human donors in association with KIR haplotype
    Article Snippet: Cytokine production analysis Cell culture supernatants were filtered using 0.2 μm 96-well filter plates (Corning) before detection of cytokines and chemokines using either ELISA kits (Peprotech) or combined cytokine singleplex assays (Invitrogen) on a Luminex100 , χMAPTM Technology. .. Cells were run on a BD Biosciences FACSCalibur flow cytometer and data analysed using FlowJo 7.6.4.

    Cell Culture:

    Article Title: Natural killer cells are recruited during pulmonary tuberculosis and their ex vivo responses to mycobacteria vary between healthy human donors in association with KIR haplotype
    Article Snippet: .. Cytokine production analysis Cell culture supernatants were filtered using 0.2 μm 96-well filter plates (Corning) before detection of cytokines and chemokines using either ELISA kits (Peprotech) or combined cytokine singleplex assays (Invitrogen) on a Luminex100 , χMAPTM Technology. .. For intracellular cytokine detection, NK cells were stimulated for 24 h. Brefeldin A (BioLegend) was added during the last 6 h of culture before harvesting cells for antibody staining with anti-CD16-FITC (Miltenyi Biotec) and anti-CD56-PC7 (BD Bioscience), then fixed and permeabilized using BD Cytofix/Cytoperm™ buffer and stained with anti-IFNγ-PE (BD Biosciences).