Structured Review

Millipore cygb
Cygb, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cygb/product/Millipore
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
cygb - by Bioz Stars, 2020-08
90/100 stars

Images

Related Articles

Negative Control:

Article Title: Putative tumor suppressor cytoglobin promotes aryl hydrocarbon receptor ligand-mediated triple negative breast cancer cell death
Article Snippet: .. Lentiviral particles containing short hairpin RNAs (shRNAs) against CYGB or green fluorescent protein (GFP), which served as negative control (Sigma Aldrich), were used to create shCYGB MDA-MB-468 cells and shGFP MDA-MB-468 respectively as described previously [ ]. .. The shCYGB sequence corresponded with 5’-CCGG-CCACAGTTGCTTTGAGCACAT-CTCGAG-ATGTGCTCAAAGCAACTGTGG-TTTTTG-3’ (Sigma Aldrich).

Multiple Displacement Amplification:

Article Title: Putative tumor suppressor cytoglobin promotes aryl hydrocarbon receptor ligand-mediated triple negative breast cancer cell death
Article Snippet: .. Lentiviral particles containing short hairpin RNAs (shRNAs) against CYGB or green fluorescent protein (GFP), which served as negative control (Sigma Aldrich), were used to create shCYGB MDA-MB-468 cells and shGFP MDA-MB-468 respectively as described previously [ ]. .. The shCYGB sequence corresponded with 5’-CCGG-CCACAGTTGCTTTGAGCACAT-CTCGAG-ATGTGCTCAAAGCAACTGTGG-TTTTTG-3’ (Sigma Aldrich).

Mouse Assay:

Article Title: Absence of cytoglobin promotes multiple organ abnormalities in aged mice
Article Snippet: .. A subgroup of WT and Cygb −/− mice at 12 weeks of age, n = 10 each group, received L-NAME (Sigma-Aldrich, Tokyo, Japan) treatment at the dose of 0.01 mg/mL in drinking water. .. Untreated mice were used as control.

Expressing:

Article Title: Cytoglobin regulates blood pressure and vascular tone through nitric oxide metabolism in the vascular wall
Article Snippet: .. The expression plasmid for Cygb (human Cygb cDNA in pET3ac, Novagen, Merck KGaA, Darmstadt, Germany) was obtained from Thorsten Burmester (Institute of Zoology and Zoological Museum, University of Hamburg, Germany) and transformed into Escherichia coli strain C41(DE3)pLysS. ..

Transformation Assay:

Article Title: Cytoglobin regulates blood pressure and vascular tone through nitric oxide metabolism in the vascular wall
Article Snippet: .. The expression plasmid for Cygb (human Cygb cDNA in pET3ac, Novagen, Merck KGaA, Darmstadt, Germany) was obtained from Thorsten Burmester (Institute of Zoology and Zoological Museum, University of Hamburg, Germany) and transformed into Escherichia coli strain C41(DE3)pLysS. ..

Plasmid Preparation:

Article Title: Cytoglobin regulates blood pressure and vascular tone through nitric oxide metabolism in the vascular wall
Article Snippet: .. The expression plasmid for Cygb (human Cygb cDNA in pET3ac, Novagen, Merck KGaA, Darmstadt, Germany) was obtained from Thorsten Burmester (Institute of Zoology and Zoological Museum, University of Hamburg, Germany) and transformed into Escherichia coli strain C41(DE3)pLysS. ..

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 85
    Millipore cygb transcription
    <t>Calcineurin</t> enhances AP-1 binding to the endogenous cytoglobin promoter. A–C , utilizing EMSA, AP-1 was demonstrated to bind to the endogenous <t>Cygb</t> promoter. The binding affinity of AP-1 to DNA was enhanced in the presence of CnA* and in response
    Cygb Transcription, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cygb transcription/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cygb transcription - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    85
    Millipore human cygb
    <t>Calcineurin</t> enhances AP-1 binding to the endogenous cytoglobin promoter. A–C , utilizing EMSA, AP-1 was demonstrated to bind to the endogenous <t>Cygb</t> promoter. The binding affinity of AP-1 to DNA was enhanced in the presence of CnA* and in response
    Human Cygb, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human cygb/product/Millipore
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    human cygb - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    Image Search Results


    Calcineurin enhances AP-1 binding to the endogenous cytoglobin promoter. A–C , utilizing EMSA, AP-1 was demonstrated to bind to the endogenous Cygb promoter. The binding affinity of AP-1 to DNA was enhanced in the presence of CnA* and in response

    Journal: The Journal of Biological Chemistry

    Article Title: Calcineurin Activates Cytoglobin Transcription in Hypoxic Myocytes

    doi: 10.1074/jbc.M809572200

    Figure Lengend Snippet: Calcineurin enhances AP-1 binding to the endogenous cytoglobin promoter. A–C , utilizing EMSA, AP-1 was demonstrated to bind to the endogenous Cygb promoter. The binding affinity of AP-1 to DNA was enhanced in the presence of CnA* and in response

    Article Snippet: In experiments designed to assess the effect of inhibiting calcineurin activity on Cygb transcription, the cells were either co-transfected with a CMV-driven Rcan1 expression plasmid or treated with cyclosporin A (CsA, 10 μ m ; Calbiochem).

    Techniques: Binding Assay

    Calcineurin regulates the transcriptional activity of the 5′ upstream region of the cytoglobin gene. A , depiction of two regions (4.6- and 0.6-kb fragments) upstream of the 5′-end of the Cygb gene illustrates the evolutionarily conserved

    Journal: The Journal of Biological Chemistry

    Article Title: Calcineurin Activates Cytoglobin Transcription in Hypoxic Myocytes

    doi: 10.1074/jbc.M809572200

    Figure Lengend Snippet: Calcineurin regulates the transcriptional activity of the 5′ upstream region of the cytoglobin gene. A , depiction of two regions (4.6- and 0.6-kb fragments) upstream of the 5′-end of the Cygb gene illustrates the evolutionarily conserved

    Article Snippet: In experiments designed to assess the effect of inhibiting calcineurin activity on Cygb transcription, the cells were either co-transfected with a CMV-driven Rcan1 expression plasmid or treated with cyclosporin A (CsA, 10 μ m ; Calbiochem).

    Techniques: Activity Assay

    Calcineurin activity increases cytoglobin levels in C2C12 cells and transgenic murine hearts. A , under normoxic conditions, Western blot analysis demonstrated an increase in Cygb protein levels in C2C12 myoblasts transfected with a CnA* expression

    Journal: The Journal of Biological Chemistry

    Article Title: Calcineurin Activates Cytoglobin Transcription in Hypoxic Myocytes

    doi: 10.1074/jbc.M809572200

    Figure Lengend Snippet: Calcineurin activity increases cytoglobin levels in C2C12 cells and transgenic murine hearts. A , under normoxic conditions, Western blot analysis demonstrated an increase in Cygb protein levels in C2C12 myoblasts transfected with a CnA* expression

    Article Snippet: In experiments designed to assess the effect of inhibiting calcineurin activity on Cygb transcription, the cells were either co-transfected with a CMV-driven Rcan1 expression plasmid or treated with cyclosporin A (CsA, 10 μ m ; Calbiochem).

    Techniques: Activity Assay, Transgenic Assay, Western Blot, Transfection, Expressing

    Site-directed mutagenesis of the AP-1 site blunts transcriptional activation of cytoglobin in response to activated calcineurin and hypoxia. The ARE within the 0.1-kb upstream fragment of the Cygb gene was either deleted or mutated. Deletion (Δ)

    Journal: The Journal of Biological Chemistry

    Article Title: Calcineurin Activates Cytoglobin Transcription in Hypoxic Myocytes

    doi: 10.1074/jbc.M809572200

    Figure Lengend Snippet: Site-directed mutagenesis of the AP-1 site blunts transcriptional activation of cytoglobin in response to activated calcineurin and hypoxia. The ARE within the 0.1-kb upstream fragment of the Cygb gene was either deleted or mutated. Deletion (Δ)

    Article Snippet: In experiments designed to assess the effect of inhibiting calcineurin activity on Cygb transcription, the cells were either co-transfected with a CMV-driven Rcan1 expression plasmid or treated with cyclosporin A (CsA, 10 μ m ; Calbiochem).

    Techniques: Mutagenesis, Activation Assay