cygb sirna  (Thermo Fisher)


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    Structured Review

    Thermo Fisher cygb sirna
    <t>CYGB</t> -knocked down melanoma cells increase proliferation. (A) Immunoblot data for C32TG and G361 cells transfected with CYGB <t>siRNA</t> or control siRNA. β-actin was used as a loading control. (B) Cellular proliferation pattern for G361 and C32TG cells transfected with CYGB siRNA (si_CYGB) and control siRNA (si_Control). The MTT analysis was performed daily (1d to 4d) post-transfection. The value represents the mean from three independent experiments; OD value, 570 nm. bars, SEM. * P
    Cygb Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cygb sirna/product/Thermo Fisher
    Average 86 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    cygb sirna - by Bioz Stars, 2020-08
    86/100 stars

    Images

    1) Product Images from "Melanoma Transition Is Frequently Accompanied by a Loss of Cytoglobin Expression in Melanocytes: A Novel Expression Site of Cytoglobin"

    Article Title: Melanoma Transition Is Frequently Accompanied by a Loss of Cytoglobin Expression in Melanocytes: A Novel Expression Site of Cytoglobin

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0094772

    CYGB -knocked down melanoma cells increase proliferation. (A) Immunoblot data for C32TG and G361 cells transfected with CYGB siRNA or control siRNA. β-actin was used as a loading control. (B) Cellular proliferation pattern for G361 and C32TG cells transfected with CYGB siRNA (si_CYGB) and control siRNA (si_Control). The MTT analysis was performed daily (1d to 4d) post-transfection. The value represents the mean from three independent experiments; OD value, 570 nm. bars, SEM. * P
    Figure Legend Snippet: CYGB -knocked down melanoma cells increase proliferation. (A) Immunoblot data for C32TG and G361 cells transfected with CYGB siRNA or control siRNA. β-actin was used as a loading control. (B) Cellular proliferation pattern for G361 and C32TG cells transfected with CYGB siRNA (si_CYGB) and control siRNA (si_Control). The MTT analysis was performed daily (1d to 4d) post-transfection. The value represents the mean from three independent experiments; OD value, 570 nm. bars, SEM. * P

    Techniques Used: Transfection, MTT Assay

    CYGB protects G361 cells from H 2 O 2 -induced cell death. A flow cytometric analysis was performed to determine the ROS level (A) and apoptosis (B) in G361 cells transfected with a CYGB-siRNA or a control-siRNA. In (A) , 2′-, 7′-dichlorofluorescein diacetate (DCFH-DA) was used. CYGB-knocked down G361 cells treated with 3 mM N-acetyl-L-cystein (NAC) were also compared. (B) Annexin V and PI staining was done after exposure of the cells to 100 μM H 2 O 2 for 0 h (−) or 24 h (+). At an early stage of apoptosis the cells bind to Annexin V while still excluding PI. At a late stage of apoptosis they bind to Annexin V and stain brightly with PI. (a) Control-siRNA-transfected, and (b) CYGB-siRNA-transfected G361 cells.
    Figure Legend Snippet: CYGB protects G361 cells from H 2 O 2 -induced cell death. A flow cytometric analysis was performed to determine the ROS level (A) and apoptosis (B) in G361 cells transfected with a CYGB-siRNA or a control-siRNA. In (A) , 2′-, 7′-dichlorofluorescein diacetate (DCFH-DA) was used. CYGB-knocked down G361 cells treated with 3 mM N-acetyl-L-cystein (NAC) were also compared. (B) Annexin V and PI staining was done after exposure of the cells to 100 μM H 2 O 2 for 0 h (−) or 24 h (+). At an early stage of apoptosis the cells bind to Annexin V while still excluding PI. At a late stage of apoptosis they bind to Annexin V and stain brightly with PI. (a) Control-siRNA-transfected, and (b) CYGB-siRNA-transfected G361 cells.

    Techniques Used: Flow Cytometry, Transfection, Staining

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Endothelial cell-induced cytoglobin expression in vascular smooth muscle cells contributes to modulation of nitric oxide
    Article Snippet: .. Efficiency of knockdown was assessed using qPCR ( and ) and western blot ( ). siRNAs against NOTCH3 were synthesized by IDT: 5′-AAC UGC GAA GUG AAC AUU G, and was used as previously described , NOTCH2 from Qiagen (Assay ID:GS4853), CYGB siRNA from ThermoFisher (Assay ID:s41571). .. All siRNAs were transfected at 100nM.

    Western Blot:

    Article Title: Endothelial cell-induced cytoglobin expression in vascular smooth muscle cells contributes to modulation of nitric oxide
    Article Snippet: .. Efficiency of knockdown was assessed using qPCR ( and ) and western blot ( ). siRNAs against NOTCH3 were synthesized by IDT: 5′-AAC UGC GAA GUG AAC AUU G, and was used as previously described , NOTCH2 from Qiagen (Assay ID:GS4853), CYGB siRNA from ThermoFisher (Assay ID:s41571). .. All siRNAs were transfected at 100nM.

    Transfection:

    Article Title: Melanoma Transition Is Frequently Accompanied by a Loss of Cytoglobin Expression in Melanocytes: A Novel Expression Site of Cytoglobin
    Article Snippet: .. Measurement of ROS Production Cells were transfected with CYGB siRNA or control siRNA and ROS generation was detected using 2′-, 7′-dichlorofluorescein diacetate (DCFH-DA) (Invitrogen). .. The cells were incubated with 10 μM of DCFH-DA for 30 min at 37°C and washed twice with PBS.

    Synthesized:

    Article Title: Endothelial cell-induced cytoglobin expression in vascular smooth muscle cells contributes to modulation of nitric oxide
    Article Snippet: .. Efficiency of knockdown was assessed using qPCR ( and ) and western blot ( ). siRNAs against NOTCH3 were synthesized by IDT: 5′-AAC UGC GAA GUG AAC AUU G, and was used as previously described , NOTCH2 from Qiagen (Assay ID:GS4853), CYGB siRNA from ThermoFisher (Assay ID:s41571). .. All siRNAs were transfected at 100nM.

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    Thermo Fisher cygb sirna
    Cygb Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cygb sirna/product/Thermo Fisher
    Average 86 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    cygb sirna - by Bioz Stars, 2020-08
    86/100 stars
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