cyclin d1  (Danaher Inc)

Journal: Molecular Medicine Reports

Article Title: Triptolide reverses cis‑diamminedichloroplatinum resistance in esophageal squamous cell carcinoma by suppressing glycolysis and causing mitochondrial malfunction

doi: 10.3892/mmr.2025.13439

Figure Lengend Snippet: TPL enhances CDDP-promoted CDDP-resistant ESCC cell cycle blockade. (A-C) Cell cycle arrest in TE-1/CDDP and KYSE30/CDDP cells was observed using a flow cytometric assay under various treatment conditions. (D-F) Differences in the level of cell cycle proteins in TE-1/CDDP and KYSE30/CDDP cells under various treatment conditions. *P<0.05. TPL, triptolide; CDDP, cis-diamminedichloro-platinum; ESCC, esophageal squamous cell carcinoma; TE-1/CDDP, cisplatin-resistant TE-1 cells; KYSE30/CDDP, cisplatin-resistant KYSE30 cells; CDK4, cyclin-dependent kinase 4.

Article Snippet: Afterwards, rabbit anti-cyclin-dependent kinase 4 (CDK4; 1:2,000; cat. no. ab199728), cyclin D1 (1:100; cat. no. ab16663), Bcl-2 (1:2,000; cat. no. ab182858), cleaved caspase-3 (1:500; cat. no. ab32042), cleaved caspase-9 (1:1,000; cat. no. 9505; CST, MA, USA), E-cadherin (1:1,000; cat. no. ab212059), Vimentin (1:1,000; cat. no. ab137321), Snail (1:1,000; cat. no. ab216347), glucose transporter protein 1 (GLUT1; 1:100,000; cat. no. ab115730), HK2 (1:1,000; cat. no. ab209847), lactate dehydrogenase A (LDHA; 1:1,000; cat. no. 2012S; CST), cytochrome c (Cytc; 1:5,000; cat. no. ab133504), COX IV (1:2,000; cat. no. ab202554) and β-actin (1:1,000; cat. no. ab8227) primary antibodies were added according to the experimental design and incubated at 4°C overnight.

Techniques: Flow Cytometry

Journal: Discover Oncology

Article Title: Inhibition of hemangioma development by regulating the VEGF/VEGFR autocrine loop via the miR-494/PTEN pathway

doi: 10.1007/s12672-025-01802-1

Figure Lengend Snippet: Effect of OMT on the expression of proteins associated with proliferation and apoptosis. Western blot was used to detect the protein expression of Cyclin D1, Bcl-2, Bax and Cleaved caspase-3. Gray value analysis of the target protein relative to β-actin. Data were expressed as mean ± standard error, P < 0.05, P < 0.01

Article Snippet: The cells were blocked with 5% skim milk powder for 1 h, followed by the addition of primary antibodies Cyclin D1 (Catalog No. 2926, 1:1000), Bcl-2 (Catalog No. 2870, 1:1000), Bax (Catalog No. 2772, 1:1000), Cleaved Caspase-3 (Catalog No. 9661, 1:1000), PTEN (Catalog No. 9188, 1:1000), VEGFR-2 (Catalog No. 2479, 1:1000), p-VEGFR-2 (Catalog No. 2478, 1:1000), ERK (Catalog No. 9102, 1:1000), p-ERK (Catalog No. 4370, 1:1000), and β-actin (Catalog No. 4970, 1:1000), purchased from Cell Signaling Technology) and incubated overnight at 4° C. The corresponding HRP-labeled secondary antibody was added and incubated for 1 h at room temperature.

Techniques: Expressing, Western Blot