Journal: Epigenomes

Article Title: Genome-Wide Methylation Profiling of Peripheral T–Cell Lymphomas Identifies TRIP13 as a Critical Driver of Tumor Proliferation and Survival

doi: 10.3390/epigenomes8030032

Figure Lengend Snippet: The treatment of T8ML-1 cells with DCZ0415 inhibits cellular growth by inducing G2-M arrest and cell death. ( A ) T8ML-1 cells were treated with either DMSO (vehicle) or DCZ0415 at indicated concentrations for 15 days and counted using Trypan Blue exclusion dye. Bar graphs represent the relative percent count of live cells after 4, 7, 10, and 15 days of continuous drug treatment. Each data point is the average of three measurements taken in parallel and bar graphs represent ±SEM. ( B ) Representative FACS diagrams of the BrdU incorporation assay of T8ML-1 cells treated with either DMSO, 5 or 10 μM of DCZ0415 after 96 and 120 h. Numbers in corners of FACS diagrams represent percentages obtained from quadrant statistics using FlowJo X software. ( C ) Percentage of cells in various phases of the cell cycle in T8ML-1 cells treated with either DMSO, 5 or 10 μM of DCZ0415 for 96 and 120 h. The numbers used to generate stacked graphs were obtained by analysis shown in ( B ) using the FACS data obtained from the BrdU incorporation assay coupled with 7-AAD staining and analyzed by FlowJo X software 10.0.7r2. ( D ) T8ML-1 cells were treated with either DMSO (vehicle) or DCZ0415 at indicated concentrations and counted using Trypan Blue exclusion dye. Bar graphs represent the total cell counts of live cells after 48 h (blue) or 72 h (red) of continuous drug treatment. Each data point is the average of three measurements taken in parallel and bar graphs represent ±SEM. ( E ) Immunoblot analysis of Cyclin B1 and Cyclin D1 protein levels in the T8ML-1 cell line treated with either DMSO (vehicle) or 10 uM DCZ0415 harvested and analyzed after 48 and 72 h of continuous drug treatment. HSC70 served as a loading control.

Article Snippet: Immunoblots were performed as previously described [ ], using the following antibodies detecting: TRIP13 (cat.# TA809737S, Origene; dilution 1:1000), Cyclin B1 (cat.# 4138, Cell Signaling; dilution 1:1000), Cyclin D1 (cat.# 2978, Cell Signaling; dilution 1:1000), Cdc25A (cat.# SC-97, Santa Cruz; dilution 1:500) and HSC70 (cat.# SC-7298, Santa Cruz; dilution 1:10,000).

Techniques: BrdU Incorporation Assay, Software, Staining, Western Blot, Control

Journal: Frontiers in Endocrinology

Article Title: Role of HNF4alpha-cMyc interaction in liver regeneration after partial hepatectomy

doi: 10.3389/fendo.2024.1404318

Figure Lengend Snippet: Primers used in this study.

Article Snippet: HNF4α (Perseus Proteomics, PP-H1415-00) and cMyc (Cell Signaling Technology, 56055), Cyclin D1 (Cell Signaling Technology, 2978), and GAPDH (Cell Signaling Technology, 2118) antibodies were used.

Techniques:

Journal: Frontiers in Endocrinology

Article Title: Role of HNF4alpha-cMyc interaction in liver regeneration after partial hepatectomy

doi: 10.3389/fendo.2024.1404318

Figure Lengend Snippet: DKO mice show disruption in cell proliferation kinetics. (A) Western blot analysis of HNF4α, cMyc and cyclin D1, and (B) qPCR analysis of Ccnd1 in WT, HNF4α-KO, cMyc-KO and DKO mice after 0h, 48h, 7D, and 14D after PHX. (C) Representative photomicrographs immunohistochemistry analysis of HNF4α and CyclinD1 in HNF4α-KO and DKO mice 14 days after PHX. Bars represent means ± SEM. n = 3 to 5. Original magnification 400x. Significant change in comparison with 0h=a, 48h=b, and 7D=c.

Article Snippet: HNF4α (Perseus Proteomics, PP-H1415-00) and cMyc (Cell Signaling Technology, 56055), Cyclin D1 (Cell Signaling Technology, 2978), and GAPDH (Cell Signaling Technology, 2118) antibodies were used.

Techniques: Disruption, Western Blot, Immunohistochemistry, Comparison

Journal: The Prostate

Article Title: Inverse agonism at the Na/K-ATPase receptor reverses EMT in prostate cancer cells

doi: 10.1002/pros.24144

Figure Lengend Snippet: Antibodies and Reagents

Article Snippet: Antibody Property Supplier Catalogue number dilution α1 NKA antibody (α6F) Mouse monoclonal Developmental Studies Hybridoma Bank of University of Iowa (Iowa) a6f 1:1000 PhosphoSrc (Tyr419) antibody Rabbit polyclonal Invitrogen 44–660G 1:1000 c-Src B-12 antibody Mouse monoclonal Santacruz Biotechnology sc-8056 1:1000 Rat α1 NKA antibody Rabbit polyclonal Dr. T.A.Pressley (Texas Tech University, TX) Not applicable 1:1000 Anti-phosphotyrosine antibody, clone 4G10 Mouse monoclonal EMD Millipore 05–321 1:1000 c-Myc antibody Santacruz Biotechnology 1:1000 Anti-tubulin antibody Mouse monoclonal SIGMA T5168 1:2000 Cyclin D1 antibody Rabbit monoclonal Cell Signaling Technology 2978S 1:1000 Cyclin E1 antibody Rabbit monoclonal Cell Signaling Technology 20808S 1:1000 p53 antibody Mouse pantropic Calbiochem OP43 1:1000 p21 antibody Rabbit polyclonal Santacruz Biotechnology sc-397 1:1000 Phospho MAPK antibody Rabbit Cell Signaling Technology 9101 1:1000 ERK1/2 antibody Rabbit polyclonal Santacruz Biotechnology sc-94 1:1000 Phospho-FAK (Tyr576/7) antibody Rabbit polyclonal Cell Signaling Technology 3281S 1:1000 FAK antibody Rabbit polyclonal Cell Signaling Technology 3285S 1:1000 Phospho-FAK (Tyr 397) antibody Rabbit polyclonal Cell Signaling Technology 3283S 1:1000 Anti-Na+/K+-ATPase β1 antibody clone 464.8 Mouse monoclonal EMD Millipore 05–382 1:1000 E-cadherin (24E10) antibody Rabbit monoclonal Cell Signaling Technology 3195S 1:1000 Anti β-catenin antibody Mouse monoclonal BD Bioscience 610153 1:1000 ZO-1 antibody Rabbit polyclonal Thermo-Fisher Scientific 61–7300 1:1000 ZO-2 antibody Rabbit polyclonal Thermo-Fisher Scientific 38–9100 1:1000 Occludin antibody (OC-3F10) Mouse monoclonal Thermo-Fisher Scientific 33–1500 1:1000 SNAIL (C15D3) antibody Rabbit monoclonal Cell Signaling Technology 3879S 1:1000 TCF8/ZEB1 antibody Rabbit monoclonal Cell Signaling Technology 3396S 1:1000 Vimentin (D21H3) antibody Rabbit monoclonal Cell Signaling Technology 5741S 1:1000 MMP-2 antibody Rabbit monoclonal Cell Signaling Technology 87809S 1:1000 MMP-9 antibody Rabbit monoclonal Cell Signaling Technology 13667S 1:1000 PCNA antibody Mouse monoclonal Santacruz Biotechnology sc-56 1:2000 Lamin B antibody Goat polyclonal Santacruz Biotechnology sc-6216 1:1000 β actin antibody Mouse monoclonal Santacruz Biotechnology sc-47778 1:1000 SLUG (C19G7) antibody Rabbit monoclonal Cell Signaling Technology 9585S 1:1000 N cadherin (D4R1H) antibody Rabbit monoclonal Cell Signaling Technology 13116S 1:1000 Open in a separate window Antibodies and Reagents Rat α1 NKA- specific antibody (NASE) was a kind gift from Dr. T. A. Pressley (Texas Tech University, TX) All reagents were obtained from Sigma-Aldrich except FAK inhibitor (Cat. No. 324877; Millipore).

Techniques:

Journal: The Prostate

Article Title: Inverse agonism at the Na/K-ATPase receptor reverses EMT in prostate cancer cells

doi: 10.1002/pros.24144

Figure Lengend Snippet: Antibodies and Reagents

Article Snippet: Antibody Property Supplier Catalogue number dilution α1 NKA antibody (α6F) Mouse monoclonal Developmental Studies Hybridoma Bank of University of Iowa (Iowa) a6f 1:1000 PhosphoSrc (Tyr419) antibody Rabbit polyclonal Invitrogen 44–660G 1:1000 c-Src B-12 antibody Mouse monoclonal Santacruz Biotechnology sc-8056 1:1000 Rat α1 NKA antibody Rabbit polyclonal Dr. T.A.Pressley (Texas Tech University, TX) Not applicable 1:1000 Anti-phosphotyrosine antibody, clone 4G10 Mouse monoclonal EMD Millipore 05–321 1:1000 c-Myc antibody Santacruz Biotechnology 1:1000 Anti-tubulin antibody Mouse monoclonal SIGMA T5168 1:2000 Cyclin D1 antibody Rabbit monoclonal Cell Signaling Technology 2978S 1:1000 Cyclin E1 antibody Rabbit monoclonal Cell Signaling Technology 20808S 1:1000 p53 antibody Mouse pantropic Calbiochem OP43 1:1000 p21 antibody Rabbit polyclonal Santacruz Biotechnology sc-397 1:1000 Phospho MAPK antibody Rabbit Cell Signaling Technology 9101 1:1000 ERK1/2 antibody Rabbit polyclonal Santacruz Biotechnology sc-94 1:1000 Phospho-FAK (Tyr576/7) antibody Rabbit polyclonal Cell Signaling Technology 3281S 1:1000 FAK antibody Rabbit polyclonal Cell Signaling Technology 3285S 1:1000 Phospho-FAK (Tyr 397) antibody Rabbit polyclonal Cell Signaling Technology 3283S 1:1000 Anti-Na+/K+-ATPase β1 antibody clone 464.8 Mouse monoclonal EMD Millipore 05–382 1:1000 E-cadherin (24E10) antibody Rabbit monoclonal Cell Signaling Technology 3195S 1:1000 Anti β-catenin antibody Mouse monoclonal BD Bioscience 610153 1:1000 ZO-1 antibody Rabbit polyclonal Thermo-Fisher Scientific 61–7300 1:1000 ZO-2 antibody Rabbit polyclonal Thermo-Fisher Scientific 38–9100 1:1000 Occludin antibody (OC-3F10) Mouse monoclonal Thermo-Fisher Scientific 33–1500 1:1000 SNAIL (C15D3) antibody Rabbit monoclonal Cell Signaling Technology 3879S 1:1000 TCF8/ZEB1 antibody Rabbit monoclonal Cell Signaling Technology 3396S 1:1000 Vimentin (D21H3) antibody Rabbit monoclonal Cell Signaling Technology 5741S 1:1000 MMP-2 antibody Rabbit monoclonal Cell Signaling Technology 87809S 1:1000 MMP-9 antibody Rabbit monoclonal Cell Signaling Technology 13667S 1:1000 PCNA antibody Mouse monoclonal Santacruz Biotechnology sc-56 1:2000 Lamin B antibody Goat polyclonal Santacruz Biotechnology sc-6216 1:1000 β actin antibody Mouse monoclonal Santacruz Biotechnology sc-47778 1:1000 SLUG (C19G7) antibody Rabbit monoclonal Cell Signaling Technology 9585S 1:1000 N cadherin (D4R1H) antibody Rabbit monoclonal Cell Signaling Technology 13116S 1:1000 Open in a separate window Antibodies and Reagents Rat α1 NKA- specific antibody (NASE) was a kind gift from Dr. T. A. Pressley (Texas Tech University, TX) All reagents were obtained from Sigma-Aldrich except FAK inhibitor (Cat. No. 324877; Millipore).

Techniques: