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Agilent technologies cy5 labeled crna
Cy5 Labeled Crna, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cy5 labeled crna/product/Agilent technologies
Average 91 stars, based on 26 article reviews
Price from $9.99 to $1999.99
cy5 labeled crna - by Bioz Stars, 2020-08
91/100 stars

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Amplification:

Article Title: Mammalian E4 Is Required for Cardiac Development and Maintenance of the Nervous System
Article Snippet: .. Linearly amplified Cy3- or Cy5-labeled cRNA was purified, fragmented, and subjected to hybridization with Agilent Mouse Development Oligonucleotide Microarrays with the use of an in situ hybridization kit (Agilent). .. The arrays were then washed first with nonstringent buffer (6× standard saline citrate and 0.005% Triton X-102) and then with stringent buffer (0.1× standard saline citrate and 0.005% Triton X-102) and were then scanned with an Agilent DNA Microarray scanner.

Blocking Assay:

Article Title: Beta-carotene affects gene expression in lungs of male and female Bcmo1−/− mice in opposite directions
Article Snippet: .. Individual 825 ng Cy5-labeled cRNA and 825 ng pooled Cy3-labeled cRNA were fragmented in 1× fragmentation and 1× blocking agent (Agilent Technologies) at 60°C for 30 min and thereafter mixed with GEx Hybridization Buffer HI-RPM (Agilent Technologies) and hybridized in a 1:1 ratio at 65°C for 17 h in an Agilent Microarray hybridization Chamber rotating at 4 rpm. ..

Purification:

Article Title: Mammalian E4 Is Required for Cardiac Development and Maintenance of the Nervous System
Article Snippet: .. Linearly amplified Cy3- or Cy5-labeled cRNA was purified, fragmented, and subjected to hybridization with Agilent Mouse Development Oligonucleotide Microarrays with the use of an in situ hybridization kit (Agilent). .. The arrays were then washed first with nonstringent buffer (6× standard saline citrate and 0.005% Triton X-102) and then with stringent buffer (0.1× standard saline citrate and 0.005% Triton X-102) and were then scanned with an Agilent DNA Microarray scanner.

Microarray:

Article Title: Beta-carotene affects gene expression in lungs of male and female Bcmo1−/− mice in opposite directions
Article Snippet: .. Individual 825 ng Cy5-labeled cRNA and 825 ng pooled Cy3-labeled cRNA were fragmented in 1× fragmentation and 1× blocking agent (Agilent Technologies) at 60°C for 30 min and thereafter mixed with GEx Hybridization Buffer HI-RPM (Agilent Technologies) and hybridized in a 1:1 ratio at 65°C for 17 h in an Agilent Microarray hybridization Chamber rotating at 4 rpm. ..

Incubation:

Article Title: Interaction Transcriptome Analysis Identifies Magnaporthe oryzae BAS1-4 as Biotrophy-Associated Secreted Proteins in Rice Blast Disease [W] BAS1-4 as Biotrophy-Associated Secreted Proteins in Rice Blast Disease [W] [OA]
Article Snippet: .. For hybridization, the labeled samples (1 μg each Cy3- and Cy5-labeled cRNA) were fragmented by the addition of 25× Agilent Fragmentation buffer and incubated for 30 min at 60°C. .. The sample was adjusted to a final volume of 450 μL with formamide-containing hybridization buffer ( ) and then added to the microarray slides.

Article Title: Gene Expression Profiling of Multiple Sclerosis Pathology Identifies Early Patterns of Demyelination Surrounding Chronic Active Lesions
Article Snippet: .. Prior to hybridization, 825 ng Cy3- and Cy5-labeled cRNA samples were fragmented by 30 min incubation at 60°C in 1× fragmentation buffer (Agilent Technologies). .. Each time, one Cy5-labeled experimental sample and one Cy3-labeled reference pool sample were hybridized to an Agilent Human Gene Expression 4 × 44K v2 Microarray (Part Number G4845A) for 17 h at 65°C in a rotating hybridization chamber.

Labeling:

Article Title: Interaction Transcriptome Analysis Identifies Magnaporthe oryzae BAS1-4 as Biotrophy-Associated Secreted Proteins in Rice Blast Disease [W] BAS1-4 as Biotrophy-Associated Secreted Proteins in Rice Blast Disease [W] [OA]
Article Snippet: .. For hybridization, the labeled samples (1 μg each Cy3- and Cy5-labeled cRNA) were fragmented by the addition of 25× Agilent Fragmentation buffer and incubated for 30 min at 60°C. .. The sample was adjusted to a final volume of 450 μL with formamide-containing hybridization buffer ( ) and then added to the microarray slides.

Article Title: Identification of Prognostic Genes for Recurrent Risk Prediction in Triple Negative Breast Cancer Patients in Taiwan
Article Snippet: .. Total RNA (500 ng) was first reverse transcribed into cDNA by incorporating a T7 oligo-dT promoter primer prior to the generation of fluorescent Cy5-labeled cRNA using an Agilent Quick Amp Labeling Kit (Agilent Technologies). .. The labeled cRNA was purified using an RNeasy Mini Kit (Qiagen) and quantified using a NanoDrop ND-1000 instrument (Thermo Fisher Scientific).

In Situ Hybridization:

Article Title: Transcriptional profiling of the leaves of near-isogenic rice lines with contrasting drought tolerance at the reproductive stage in response to water deficit
Article Snippet: .. The hybridization solution was prepared with Cy3- and Cy5-labeled cRNA (825 ng each) using an in situ Hybridization Kit Plus (Agilent Technologies). .. The hybridization and washing of the microarray slides were performed according to the manufacturer’s protocols.

Article Title: Mammalian E4 Is Required for Cardiac Development and Maintenance of the Nervous System
Article Snippet: .. Linearly amplified Cy3- or Cy5-labeled cRNA was purified, fragmented, and subjected to hybridization with Agilent Mouse Development Oligonucleotide Microarrays with the use of an in situ hybridization kit (Agilent). .. The arrays were then washed first with nonstringent buffer (6× standard saline citrate and 0.005% Triton X-102) and then with stringent buffer (0.1× standard saline citrate and 0.005% Triton X-102) and were then scanned with an Agilent DNA Microarray scanner.

Hybridization:

Article Title: Beta-carotene affects gene expression in lungs of male and female Bcmo1−/− mice in opposite directions
Article Snippet: .. Individual 825 ng Cy5-labeled cRNA and 825 ng pooled Cy3-labeled cRNA were fragmented in 1× fragmentation and 1× blocking agent (Agilent Technologies) at 60°C for 30 min and thereafter mixed with GEx Hybridization Buffer HI-RPM (Agilent Technologies) and hybridized in a 1:1 ratio at 65°C for 17 h in an Agilent Microarray hybridization Chamber rotating at 4 rpm. ..

Article Title: Gene Expression Profiling of Gastric Cancer
Article Snippet: .. Hybridization, scanning and data analysis Fragmented Cy3-labeled cRNA of the control sample was mixed with equal amounts of Cy5-labeled cRNA from the gastric tumor sample and the mixtures hybridized onto 44K whole human genome DNA microarrays (G4112F, 4x44K, Agilent Technologies) for 17 hrs at 65°C with constant rotation (10 rpm). .. The slides were scanned using an Agilent microarray scanner (G2505B), and the images processed and analyzed using the Agilent feature extraction software AFE 9.5.

Article Title: Transcriptional profiling of the leaves of near-isogenic rice lines with contrasting drought tolerance at the reproductive stage in response to water deficit
Article Snippet: .. The hybridization solution was prepared with Cy3- and Cy5-labeled cRNA (825 ng each) using an in situ Hybridization Kit Plus (Agilent Technologies). .. The hybridization and washing of the microarray slides were performed according to the manufacturer’s protocols.

Article Title: Interaction Transcriptome Analysis Identifies Magnaporthe oryzae BAS1-4 as Biotrophy-Associated Secreted Proteins in Rice Blast Disease [W] BAS1-4 as Biotrophy-Associated Secreted Proteins in Rice Blast Disease [W] [OA]
Article Snippet: .. For hybridization, the labeled samples (1 μg each Cy3- and Cy5-labeled cRNA) were fragmented by the addition of 25× Agilent Fragmentation buffer and incubated for 30 min at 60°C. .. The sample was adjusted to a final volume of 450 μL with formamide-containing hybridization buffer ( ) and then added to the microarray slides.

Article Title: Mammalian E4 Is Required for Cardiac Development and Maintenance of the Nervous System
Article Snippet: .. Linearly amplified Cy3- or Cy5-labeled cRNA was purified, fragmented, and subjected to hybridization with Agilent Mouse Development Oligonucleotide Microarrays with the use of an in situ hybridization kit (Agilent). .. The arrays were then washed first with nonstringent buffer (6× standard saline citrate and 0.005% Triton X-102) and then with stringent buffer (0.1× standard saline citrate and 0.005% Triton X-102) and were then scanned with an Agilent DNA Microarray scanner.

Article Title: Gene Expression Profiling of Multiple Sclerosis Pathology Identifies Early Patterns of Demyelination Surrounding Chronic Active Lesions
Article Snippet: .. Prior to hybridization, 825 ng Cy3- and Cy5-labeled cRNA samples were fragmented by 30 min incubation at 60°C in 1× fragmentation buffer (Agilent Technologies). .. Each time, one Cy5-labeled experimental sample and one Cy3-labeled reference pool sample were hybridized to an Agilent Human Gene Expression 4 × 44K v2 Microarray (Part Number G4845A) for 17 h at 65°C in a rotating hybridization chamber.

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