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GE Healthcare cy5 ap3 dutp
Cy5 Ap3 Dutp, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cy5 ap3 dutp/product/GE Healthcare
Average 88 stars, based on 12 article reviews
Price from $9.99 to $1999.99
cy5 ap3 dutp - by Bioz Stars, 2020-07
88/100 stars

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Amplification:

Article Title: Comparative Fitness Assessment of Anopheles stephensi Transgenic Lines Receptive to Site-Specific Integration
Article Snippet: .. Chromosome marker probes, Ski , PPO1 , and Vitellogenin (Accession nos., and , respectively) were amplified from An. stephensi genomic DNA and labeled with Cy5-AP3-dUTP (GE Healthcare). .. Polytene chromosomes from both transgenic and wild-type half-gravid females were prepared ( ) and hybridized overnight at 37°C ( ).

Modification:

Article Title: A Physical Map for an Asian Malaria Mosquito, Anopheles stephensi
Article Snippet: .. The isolated DNA was labeled with Cy5-AP3-dUTP and Cy3-AP3-dUTP (GE Healthcare UK Ltd., Buckinghamshire, England) using a modified Nick translation labeling protocol or labeled with Biotin-16-dUTP by a modified Nick Translation Mix protocol (Roche Applied Science, Indianapolis, IN). .. The DNA probes were hybridized to the chromosomes at 39°C overnight in hybridization solution (Invitrogen Corp., Carlsbad, CA).

Article Title: Chromosomal localization of actin genes in the malaria mosquito Anopheles darlingi
Article Snippet: .. We labeled the isolated DNA with Cy5-AP3-dUTP (GE Healthcare UK Ltd., Buckinghamshire, England) or with Biotin-16-dUTP using a modified Nick-Translation Mix protocol (Roche Applied Science) and hybridized to the chromosomes at 39°C overnight in hybridization solution (Invitrogen Corporation, Carlsbad, CA, USA). .. We detected fluorescent signals using an ACCORD Automatic Fluorescent Imaging System (BioView (USA), Inc., Billerica, MA, USA) and determined localization within a subdivision, using a standard cytogenetic map for An. darlingi ( ).

Isolation:

Article Title: A Physical Map for an Asian Malaria Mosquito, Anopheles stephensi
Article Snippet: .. The isolated DNA was labeled with Cy5-AP3-dUTP and Cy3-AP3-dUTP (GE Healthcare UK Ltd., Buckinghamshire, England) using a modified Nick translation labeling protocol or labeled with Biotin-16-dUTP by a modified Nick Translation Mix protocol (Roche Applied Science, Indianapolis, IN). .. The DNA probes were hybridized to the chromosomes at 39°C overnight in hybridization solution (Invitrogen Corp., Carlsbad, CA).

Article Title: Chromosomal localization of actin genes in the malaria mosquito Anopheles darlingi
Article Snippet: .. We labeled the isolated DNA with Cy5-AP3-dUTP (GE Healthcare UK Ltd., Buckinghamshire, England) or with Biotin-16-dUTP using a modified Nick-Translation Mix protocol (Roche Applied Science) and hybridized to the chromosomes at 39°C overnight in hybridization solution (Invitrogen Corporation, Carlsbad, CA, USA). .. We detected fluorescent signals using an ACCORD Automatic Fluorescent Imaging System (BioView (USA), Inc., Billerica, MA, USA) and determined localization within a subdivision, using a standard cytogenetic map for An. darlingi ( ).

Nick Translation:

Article Title: A Physical Map for an Asian Malaria Mosquito, Anopheles stephensi
Article Snippet: .. The isolated DNA was labeled with Cy5-AP3-dUTP and Cy3-AP3-dUTP (GE Healthcare UK Ltd., Buckinghamshire, England) using a modified Nick translation labeling protocol or labeled with Biotin-16-dUTP by a modified Nick Translation Mix protocol (Roche Applied Science, Indianapolis, IN). .. The DNA probes were hybridized to the chromosomes at 39°C overnight in hybridization solution (Invitrogen Corp., Carlsbad, CA).

Article Title: Breakpoint structure reveals the unique origin of an interspecific chromosomal inversion (2La) in the Anopheles gambiae complex
Article Snippet: .. Probes were prepared from 1 μg of BAC DNA or 100 ng of PCR product labeled with Cy3-AP3-dUTP or Cy5-AP3-dUTP (where AP3 is 5-amino-propargyl) (Amersham Biosciences) by using the GIBCO/BRL BioPrime DNA labeling system (Life Technologies, Gaithersburg, MD) with dNTPs from the nick translation kit (Amersham Pharmacia) in half-volume reactions. .. The in situ hybridization was performed with the GIBCO/BRL in situ hybridization and detection system, following the manufacturer's recommended protocol.

Article Title: Chromosomal localization of actin genes in the malaria mosquito Anopheles darlingi
Article Snippet: .. We labeled the isolated DNA with Cy5-AP3-dUTP (GE Healthcare UK Ltd., Buckinghamshire, England) or with Biotin-16-dUTP using a modified Nick-Translation Mix protocol (Roche Applied Science) and hybridized to the chromosomes at 39°C overnight in hybridization solution (Invitrogen Corporation, Carlsbad, CA, USA). .. We detected fluorescent signals using an ACCORD Automatic Fluorescent Imaging System (BioView (USA), Inc., Billerica, MA, USA) and determined localization within a subdivision, using a standard cytogenetic map for An. darlingi ( ).

Labeling:

Article Title: A Physical Map for an Asian Malaria Mosquito, Anopheles stephensi
Article Snippet: .. The isolated DNA was labeled with Cy5-AP3-dUTP and Cy3-AP3-dUTP (GE Healthcare UK Ltd., Buckinghamshire, England) using a modified Nick translation labeling protocol or labeled with Biotin-16-dUTP by a modified Nick Translation Mix protocol (Roche Applied Science, Indianapolis, IN). .. The DNA probes were hybridized to the chromosomes at 39°C overnight in hybridization solution (Invitrogen Corp., Carlsbad, CA).

Article Title: Next-Generation Sequencing Reveals Recent Horizontal Transfer of a DNA Transposon between Divergent Mosquitoes
Article Snippet: .. PCR products of an Asi_MJ1 transposon with confirmed sequence were labeled with Cy3 and Cy5-AP3-dUTP (GE Healthcare UK Ltd, Buckinghamshire, England) by Random Primers DNA Labeling System (Invitrogen Corporation, Carlsbad, CA, USA). .. The DNA probes were hybridized to the chromosomes at 39°C overnight in 2x hybridization buffer (Invitrogen Corporation, Carlsbad, CA, USA).

Article Title: Breakpoint structure reveals the unique origin of an interspecific chromosomal inversion (2La) in the Anopheles gambiae complex
Article Snippet: .. Probes were prepared from 1 μg of BAC DNA or 100 ng of PCR product labeled with Cy3-AP3-dUTP or Cy5-AP3-dUTP (where AP3 is 5-amino-propargyl) (Amersham Biosciences) by using the GIBCO/BRL BioPrime DNA labeling system (Life Technologies, Gaithersburg, MD) with dNTPs from the nick translation kit (Amersham Pharmacia) in half-volume reactions. .. The in situ hybridization was performed with the GIBCO/BRL in situ hybridization and detection system, following the manufacturer's recommended protocol.

Article Title: Improving the population genetics toolbox for the study of the African malaria vector Anopheles nili: microsatellite mapping to chromosomes
Article Snippet: .. Probes were labeled using Cy3-AP3-dUTP or Cy5-AP3-dUTP (GE Healthcare UK Ltd., Buckinghmashire, UK) fluorophores by a Random Primer DNA Labelling System (Invitrogen Corporation, Carlsbad, CA, USA). .. Chromosome preparation and FISH To obtain chromosomal slides, follicles of ovaries were separated in 50% propionic acid.

Article Title: Transgenic Anopheles stephensi coexpressing single-chain antibodies resist Plasmodium falciparum development
Article Snippet: .. Probes specific to each copy of the attP docking site were labeled with Cy5-AP3-dUTP (GE Healthcare). ..

Article Title: Chromosomal localization of actin genes in the malaria mosquito Anopheles darlingi
Article Snippet: .. We labeled the isolated DNA with Cy5-AP3-dUTP (GE Healthcare UK Ltd., Buckinghamshire, England) or with Biotin-16-dUTP using a modified Nick-Translation Mix protocol (Roche Applied Science) and hybridized to the chromosomes at 39°C overnight in hybridization solution (Invitrogen Corporation, Carlsbad, CA, USA). .. We detected fluorescent signals using an ACCORD Automatic Fluorescent Imaging System (BioView (USA), Inc., Billerica, MA, USA) and determined localization within a subdivision, using a standard cytogenetic map for An. darlingi ( ).

Article Title: An Integrated Chromosome Map of Microsatellite Markers and Inversion Breakpoints for an Asian Malaria Mosquito, Anopheles stephensi
Article Snippet: .. Probes were labeled using Cy3-AP3-dUTP or Cy5-AP3-dUTP (GE Healthcare UK Ltd.) fluorophores by a Random Primer DNA Labeling System (Invitrogen Corporation, Carlsbad, CA). .. Labeled probes were hybridized at 42 °C to A . stephensi polytene chromosome slides overnight.

Article Title: Comparative Fitness Assessment of Anopheles stephensi Transgenic Lines Receptive to Site-Specific Integration
Article Snippet: .. Chromosome marker probes, Ski , PPO1 , and Vitellogenin (Accession nos., and , respectively) were amplified from An. stephensi genomic DNA and labeled with Cy5-AP3-dUTP (GE Healthcare). .. Polytene chromosomes from both transgenic and wild-type half-gravid females were prepared ( ) and hybridized overnight at 37°C ( ).

Sequencing:

Article Title: Next-Generation Sequencing Reveals Recent Horizontal Transfer of a DNA Transposon between Divergent Mosquitoes
Article Snippet: .. PCR products of an Asi_MJ1 transposon with confirmed sequence were labeled with Cy3 and Cy5-AP3-dUTP (GE Healthcare UK Ltd, Buckinghamshire, England) by Random Primers DNA Labeling System (Invitrogen Corporation, Carlsbad, CA, USA). .. The DNA probes were hybridized to the chromosomes at 39°C overnight in 2x hybridization buffer (Invitrogen Corporation, Carlsbad, CA, USA).

Polymerase Chain Reaction:

Article Title: Next-Generation Sequencing Reveals Recent Horizontal Transfer of a DNA Transposon between Divergent Mosquitoes
Article Snippet: .. PCR products of an Asi_MJ1 transposon with confirmed sequence were labeled with Cy3 and Cy5-AP3-dUTP (GE Healthcare UK Ltd, Buckinghamshire, England) by Random Primers DNA Labeling System (Invitrogen Corporation, Carlsbad, CA, USA). .. The DNA probes were hybridized to the chromosomes at 39°C overnight in 2x hybridization buffer (Invitrogen Corporation, Carlsbad, CA, USA).

Article Title: Breakpoint structure reveals the unique origin of an interspecific chromosomal inversion (2La) in the Anopheles gambiae complex
Article Snippet: .. Probes were prepared from 1 μg of BAC DNA or 100 ng of PCR product labeled with Cy3-AP3-dUTP or Cy5-AP3-dUTP (where AP3 is 5-amino-propargyl) (Amersham Biosciences) by using the GIBCO/BRL BioPrime DNA labeling system (Life Technologies, Gaithersburg, MD) with dNTPs from the nick translation kit (Amersham Pharmacia) in half-volume reactions. .. The in situ hybridization was performed with the GIBCO/BRL in situ hybridization and detection system, following the manufacturer's recommended protocol.

Random Primer DNA Labeling:

Article Title: An Integrated Chromosome Map of Microsatellite Markers and Inversion Breakpoints for an Asian Malaria Mosquito, Anopheles stephensi
Article Snippet: .. Probes were labeled using Cy3-AP3-dUTP or Cy5-AP3-dUTP (GE Healthcare UK Ltd.) fluorophores by a Random Primer DNA Labeling System (Invitrogen Corporation, Carlsbad, CA). .. Labeled probes were hybridized at 42 °C to A . stephensi polytene chromosome slides overnight.

BAC Assay:

Article Title: Breakpoint structure reveals the unique origin of an interspecific chromosomal inversion (2La) in the Anopheles gambiae complex
Article Snippet: .. Probes were prepared from 1 μg of BAC DNA or 100 ng of PCR product labeled with Cy3-AP3-dUTP or Cy5-AP3-dUTP (where AP3 is 5-amino-propargyl) (Amersham Biosciences) by using the GIBCO/BRL BioPrime DNA labeling system (Life Technologies, Gaithersburg, MD) with dNTPs from the nick translation kit (Amersham Pharmacia) in half-volume reactions. .. The in situ hybridization was performed with the GIBCO/BRL in situ hybridization and detection system, following the manufacturer's recommended protocol.

DNA Labeling:

Article Title: Next-Generation Sequencing Reveals Recent Horizontal Transfer of a DNA Transposon between Divergent Mosquitoes
Article Snippet: .. PCR products of an Asi_MJ1 transposon with confirmed sequence were labeled with Cy3 and Cy5-AP3-dUTP (GE Healthcare UK Ltd, Buckinghamshire, England) by Random Primers DNA Labeling System (Invitrogen Corporation, Carlsbad, CA, USA). .. The DNA probes were hybridized to the chromosomes at 39°C overnight in 2x hybridization buffer (Invitrogen Corporation, Carlsbad, CA, USA).

Article Title: Breakpoint structure reveals the unique origin of an interspecific chromosomal inversion (2La) in the Anopheles gambiae complex
Article Snippet: .. Probes were prepared from 1 μg of BAC DNA or 100 ng of PCR product labeled with Cy3-AP3-dUTP or Cy5-AP3-dUTP (where AP3 is 5-amino-propargyl) (Amersham Biosciences) by using the GIBCO/BRL BioPrime DNA labeling system (Life Technologies, Gaithersburg, MD) with dNTPs from the nick translation kit (Amersham Pharmacia) in half-volume reactions. .. The in situ hybridization was performed with the GIBCO/BRL in situ hybridization and detection system, following the manufacturer's recommended protocol.

Marker:

Article Title: Comparative Fitness Assessment of Anopheles stephensi Transgenic Lines Receptive to Site-Specific Integration
Article Snippet: .. Chromosome marker probes, Ski , PPO1 , and Vitellogenin (Accession nos., and , respectively) were amplified from An. stephensi genomic DNA and labeled with Cy5-AP3-dUTP (GE Healthcare). .. Polytene chromosomes from both transgenic and wild-type half-gravid females were prepared ( ) and hybridized overnight at 37°C ( ).

Hybridization:

Article Title: Chromosomal localization of actin genes in the malaria mosquito Anopheles darlingi
Article Snippet: .. We labeled the isolated DNA with Cy5-AP3-dUTP (GE Healthcare UK Ltd., Buckinghamshire, England) or with Biotin-16-dUTP using a modified Nick-Translation Mix protocol (Roche Applied Science) and hybridized to the chromosomes at 39°C overnight in hybridization solution (Invitrogen Corporation, Carlsbad, CA, USA). .. We detected fluorescent signals using an ACCORD Automatic Fluorescent Imaging System (BioView (USA), Inc., Billerica, MA, USA) and determined localization within a subdivision, using a standard cytogenetic map for An. darlingi ( ).

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    GE Healthcare cy5 ap3 dutp
    Cy5 Ap3 Dutp, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cy5 ap3 dutp/product/GE Healthcare
    Average 88 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    cy5 ap3 dutp - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

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