Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Inflammation-induced LncRNA SNHG1 orchestrates spermatogonium development in non-obstructive azoospermia via IL-17 A signaling pathway

doi: 10.1007/s00018-025-06055-3

Figure Lengend Snippet: Effect of inflammation on spermatogonia in LPS-induced mouse testes. ( A ) Testes from LPS-induced mice (Orchitis) and the Sham group. n = 6 mice per group ( B ) Expression levels of SNHG1 and SP1 at the transcriptional level were detected using qPCR. n = 6 mice per group ( C ) Expression of SP1 at the protein level as detected by Western blot. n = 6 mice per group ( D ) H&E staining was used to analyze the histopathology of the testes. n = 3 mice per group ( E ) Apoptosis in testes was analyzed using TUNEL staining. n = 3 mice per group (F-I) Expression levels of Oct4, C-kit, IL-17 A and ZO-1 as detected by IHC assay. n = 3 mice per group. Statistical comparisons between the two groups were performed using an unpaired two-tailed Student’s t-test. *** P < 0.001

Article Snippet: For cell lines, the one-step TUNEL apoptosis assay kit with Cy3 (Beyotime, China) was used according to the manufacturer’s instructions.

Techniques: Expressing, Western Blot, Staining, Histopathology, TUNEL Assay, Two Tailed Test

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Inflammation-induced LncRNA SNHG1 orchestrates spermatogonium development in non-obstructive azoospermia via IL-17 A signaling pathway

doi: 10.1007/s00018-025-06055-3

Figure Lengend Snippet: Effects of LPS-induced inflammation on spermatogonia in vitro. (A) Cell proliferation was detected by EdU staining. (B-C) Apoptosis was detected using flow cytometry and the TUNEL assay. (D) SP1 expression at the protein level was detected using a Western blot. (E) Expression levels of SNHG1 and SP1 at the transcriptional level were detected using qPCR. (F–H) IF analysis of SP1 , C-kit, and Oct4 expression. All experiments were performed in at least triplicate. Statistical comparisons between the two groups were performed using an unpaired two-tailed Student’s t-test. ** P < 0.01, *** P < 0.001

Article Snippet: For cell lines, the one-step TUNEL apoptosis assay kit with Cy3 (Beyotime, China) was used according to the manufacturer’s instructions.

Techniques: In Vitro, Staining, Flow Cytometry, TUNEL Assay, Expressing, Western Blot, Two Tailed Test

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Inflammation-induced LncRNA SNHG1 orchestrates spermatogonium development in non-obstructive azoospermia via IL-17 A signaling pathway

doi: 10.1007/s00018-025-06055-3

Figure Lengend Snippet: SP1 knockdown reduces the LPS-induced spermatogonia dysfunction. ( A ) Cell proliferation was detected using EdU staining. ( B - C ) Cell apoptosis was detected using flow cytometry and the TUNEL assay. ( D ) Expression levels of SNHG1 and SP1 at the transcriptional level were detected using qPCR. ( E ) SP1 expression at the protein level was detected by Western blot. All experiments were performed in at least triplicate. Statistical significance was determined by one-way ANOVA with Tukey’s post hoc test. ns: not significant, * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: For cell lines, the one-step TUNEL apoptosis assay kit with Cy3 (Beyotime, China) was used according to the manufacturer’s instructions.

Techniques: Knockdown, Staining, Flow Cytometry, TUNEL Assay, Expressing, Western Blot

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Inflammation-induced LncRNA SNHG1 orchestrates spermatogonium development in non-obstructive azoospermia via IL-17 A signaling pathway

doi: 10.1007/s00018-025-06055-3

Figure Lengend Snippet: SNHG1 knockdown reduces LPS-induced spermatogonial dysfunction. ( A ) Cell proliferation was detected by EdU staining. ( B - C ) Cell apoptosis was detected by flow cytometry and TUNEL assay. ( D ) Expression levels of SNHG1 and SP1 at the transcriptional level were detected by qPCR. ( E ) SP1 expression at the protein level was detected by Western blot. ( F – H ) IF analysis of SP1 , C-kit, and Oct4 expression. All experiments were performed in at least triplicate. Statistical significance was determined by one-way ANOVA with Tukey’s post hoc test. ns: not significant, * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: For cell lines, the one-step TUNEL apoptosis assay kit with Cy3 (Beyotime, China) was used according to the manufacturer’s instructions.

Techniques: Knockdown, Staining, Flow Cytometry, TUNEL Assay, Expressing, Western Blot

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Inflammation-induced LncRNA SNHG1 orchestrates spermatogonium development in non-obstructive azoospermia via IL-17 A signaling pathway

doi: 10.1007/s00018-025-06055-3

Figure Lengend Snippet: IL-17A signaling pathway blockade rescued the SNHG1 -induced cell dysfunction. ( A ) Cell proliferation was detected using EdU staining. ( B - C ) Cell apoptosis detected by flow cytometry and TUNEL assay. ( D ) Expression levels of SNHG1 and SP1 at the transcriptional level were detected by qPCR. ( E ) SP1 expression at the protein level was detected using a Western blot. ( F – H ) IF analysis of SP1 , C-kit, and Oct4 expression. All experiments were performed in at least triplicate. Statistical significance was determined by one-way ANOVA with Tukey’s post hoc test. ns: not significant, ** P < 0.01, *** P < 0.001

Article Snippet: For cell lines, the one-step TUNEL apoptosis assay kit with Cy3 (Beyotime, China) was used according to the manufacturer’s instructions.

Techniques: Staining, Flow Cytometry, TUNEL Assay, Expressing, Western Blot

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Inflammation-induced LncRNA SNHG1 orchestrates spermatogonium development in non-obstructive azoospermia via IL-17 A signaling pathway

doi: 10.1007/s00018-025-06055-3

Figure Lengend Snippet: Re-expressing SNHGI in SP1-knock-down cells protect cells from inflammatory damage. ( A - C ) Expression levels of SP1 , SNHG1 , and IL-17 A were detected by QPCR assay. ( D ) The WB method was employed to assess the protein expression levels of SP1 and IL-17 A . ( E ) EdU staining was utilized to evaluate cell proliferation. ( F ) Flow cytometry was applied to detect cell apoptosis. All experiments were performed in at least triplicate. Statistical significance was determined by one-way ANOVA with Tukey’s post hoc test. ns: not significant, ** P < 0.01, *** P < 0.001

Article Snippet: For cell lines, the one-step TUNEL apoptosis assay kit with Cy3 (Beyotime, China) was used according to the manufacturer’s instructions.

Techniques: Expressing, Knockdown, Staining, Flow Cytometry